• Asian-Australasian Journal of Animal Sciences
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• 제32권11호
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• pp.1715-1724
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• 2019

Objective: As laying hens become aged, laying performance and egg quality are generally impaired. One of the practical methods to rejuvenate production and egg quality of aged laying hens with decreasing productivity is a forced molting. However, the changes in intestinal microbiota after forced molting of aged hens are not clearly known. The aim of the present study was to analyze the changes in excreta bacterial communities after forced molting of aged laying hens. Methods: A total of one hundred 66-wk-old Hy-Line Brown laying hens were induced to molt by a 2-d water removal and an 11-d fasting until egg production completely ceased. The excreta samples of 16 hens with similar body weight were collected before and immediately after molting. Excreta bacterial communities were analyzed by high-throughput sequencing of bacterial 16S rRNA genes. Results: Bacteroidetes, Firmicutes, and Proteobacteria were the three major bacterial phyla in pre-molting and immediate post-molting hens, accounting for more than 98.0%. Lactobacillus genus had relatively high abundance in both group, but decreased by molting (62.3% in premolting and 24.9% in post-molting hens). Moreover, pathogenic bacteria such as Enterococcus cecorum and Escherichia coli were more abundant in immediate post-molting hens than in pre-molting hens. Forced molting influenced the alpha diversity, with higher Chao1 (p = 0.012), phylogenetic diversity whole tree (p = 0.014), observed operational taxonomic unit indices (p = 0.006), and Simpson indices (p<0.001), which indicated that forced molting increased excreta bacterial richness of aged laying hens. Conclusion: This study improves the current knowledge of bacterial community alterations in the excreta by forced molting in aged laying hens, which can provide increasing opportunity to develop novel dietary and management skills for improving the gastrointestinal health of aged laying hens after molting.

• 실내환경 및 냄새 학회지
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• 제16권1호
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• pp.54-63
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• 2017

The Automobile HVAC system is a habitat for odor-associated fungal communities. We investigated the odor-associated fungal community in an automobile HVAC system using a high-throughput DNA sequencing method. The fungal community structure was evaluated via metagenome analysis. At the phylum level, Ascomycota and Basidiomycota were detected, accounting for 43.41% and 56.49% of the fungal community in the HVAC system, respectively. Columnosphaeria (8.31%), Didymella (5.60%), Davidiella (5.50%), Microxyphium (4.24%), unclassified Pleosporales (2.90%), and Cladosporium (2.79%) were abundant at phylum of Ascomycota and Christiansenia (36.72%), Rhodotorula (10.48%), and Sporidiobolus (2.34%) were abundant at phylum of Basidiomycota. A total of 22 genera of fungi were isolated and identified from the evaporators of the HVAC systems which support fungal growth and biofilm formation. Among them, Cladosporium, Penicillium, Aspergillus, and Alternaria are the most representative odor-associated fungi in HVAC systems. They were reported to form biofilm on the surface of HVAC systems with other bacteria by hypha. In addition, they produce various mVOCs such as 3-methyl-1-butanol, acetic acid, butanoic acid, and methyl isobutyl ketone. Our findings may be useful for extending the understanding of odor-associated fungal communities in automobile HVAC systems.

• 정보처리학회논문지C
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• 제16C권1호
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• pp.133-142
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• 2009

이 논문에서는 은닉 노드(hidden node)들이 있는 환경에서 IEEE 802.15.4의 성능을 정확하게 예측할 수 있는 수학적 모델을 제시한다. 기존의 802.15.4 성능 분석을 위한 수학적 모델은 은닉 노드들이 없는, 즉 모든 노드들은 다른 노드들의 전송 상태를 측정할 수 있는 이상적인 상황만을 고려하였다. 그러나 노드들의 배치에 따라서 은닉 노드들이 빈번하게 발생할 수 있어, 기존의 모델들은 현실적 환경에서 802.15.4의 성능을 정확히 측정하지 못하는 문제가 있다. 이러한 문제를 해결하기 위해서 이 논문에서 제안한 모델은 기존의 802.15.4의 성능 모델에 은닉노드의 성능 영향 모델을 추가하였다. 제안한 802.15.4의 수학적 성능 모델에 의하면 작은 은닉 노드의 수에 의해 성능이 급격하게 저하된다. 일례로 전체 노드의 5%가 은닉노드일 때 802.15.4의 성능이 최대 62% 저하된다. 이러한 예측한 결과는 ns-2의 시뮬레이션 결과와 비교할 때, 최대 6%의 오류한도에서 동일하다.

• Parasites, Hosts and Diseases
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• 제58권5호
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• pp.537-542
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• 2020

Cockroaches inhabit various habitats, which will influence their microbiome. Although the microbiome can be influenced by the diet and environmental factors, it can also differ between species. Therefore, we conducted 16S rDNA-targeted high-throughput sequencing to evaluate the overall bacterial composition of the microbiomes of 3 cockroach species, Periplaneta americana, P. japonica, and P. fuliginosa, raised in laboratory for several generations under the same conditions. The experiments were conducted using male adult cockroaches. The number of operational taxonomic units (OTUs) was not significantly different among the 3 species. With regard to the Shannon and Pielou indexes, higher microbiome values were noted in P. americana than in P. japonica and P. fuliginosa. Microbiome composition was also evaluated, with endosymbionts accounting for over half of all OTUs in P. japonica and P. fuliginosa. Beta diversity analysis further showed that P. japonica and P. fuliginosa had similar microbiome composition, which differed from that of P. americana. However, we also identified that P. japonica and P. fuliginosa host distinct OTUs. Thus, although microbiome compositions may vary based on multiple conditions, it is possible to identify distinct microbiome compositions among different Periplaneta cockroach species, even when the individuals are reared under the same conditions.

• 한국항만경제학회지
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• 제27권4호
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• pp.73-90
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• 2011

기업들의 국제 분업화와 SCM(supply chain management) 체계 확산을 계기로 SCM과 물류가 통합되었으며, 이러한 패러다임 변화에 의해 항만과 공항의 배후단지가 공급, 제조, 유통 등의 중심지가 되고 있다. 또한, 글로벌 SCM 체계의 확산 과정에서 글로벌 기업들은 항만 및 항만 배후부지의 경쟁력을 평가하는데 있어 입지여건을 매우 중요한 요인으로 평가하였고, 기존의 물동량 창출과 함께 배후부지에서의 부가가치물류(value added logistics)의 중요성이 강화되고 있는 상황이다. 이에 본 연구에서는 항만 배후부지의 경쟁력 평가 모형을 수립하고, 7개 국내외 항만 배후부지를 평가하고자 하였다. AHP 분석을 통해 항만 배후부지 경쟁력 평가요인들의 중요도 분석을 수행한 결과는 물류요인이 62.3%의 중요도를 보이며, 다음으로 배후경제요인(27.3%), 도시 및 정책요인(10.4%)의 순으로 분석되었다. 이러한 결과는 항만 배후부지 경쟁력 향상을 위해서는 상대적으로 중요도가 높은 물류 요소에 대한 활성화방안 마련이 가장 필수적임을 의미한다. 특히, 항만의 서비스노선과 항공 네트워크망 구축이 필요하며, 이를 지원하기 위한 인프라 확충도 뒤따라야 할 것이다. 국내외 7대 항만 배후부지 경쟁력에 대한 부산항의 평가 결과는 싱가포르, 홍콩, 상해에 이어 4위로 평가되었다. 향후 본 연구에서 제시한 항만 배후부지 경쟁력 평가모형을 바탕으로 국내 항만 배후부지의 경쟁력을 평가하여 차별화된 인센티브 제공 등에 대한 연구가 추가되어야 할 것이다.

• Animal Bioscience
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• 제35권11호
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• pp.1698-1710
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• 2022

Objective: Raw potato starch (RPS) is resistant to digestion, escapes absorption, and is metabolized by intestinal microflora in the large intestine and acts as their energy source. In this study, we compared the effect of different concentrations of RPS on the intestinal bacterial community of weaned piglets. Methods: Male weaned piglets (25-days-old, 7.03±0.49 kg) were either fed a corn/soybean-based control diet (CON, n = 6) or two treatment diets supplemented with 5% RPS (RPS5, n = 4) or 10% RPS (RPS10, n = 4) for 20 days and their fecal samples were collected. The day 0 and 20 samples were analyzed using a 16S rRNA gene sequencing technology, followed by total genomic DNA extraction, library construction, and high-throughput sequencing. After statistical analysis, five phyla and 45 genera accounting for over 0.5% of the reads in any of the three groups were further analyzed. Furthermore, short-chain fatty acids (SCFAs) in the day 20 fecal samples were analyzed using gas chromatography. Results: Significant changes were not observed in the bacterial composition at the phylum level even after 20 d post feeding (dpf); however, the abundance of Intestinimonas and Barnesiella decreased in both RPS treatment groups compared to the CON group. Consumption of 5% RPS increased the abundance of Roseburia (p<0.05) and decreased the abundance of Clostridium (p<0.01) and Mediterraneibacter (p< 0.05). In contrast, consumption of 10% RPS increased the abundance of Olsenella (p<0.05) and decreased the abundance of Campylobacter (p<0.05), Kineothrix (p<0.05), Paraprevotella (p<0.05), and Vallitalea (p<0.05). Additionally, acetate (p<0.01), butyrate (p<0.05), valerate (p = 0.01), and total SCFAs (p = 0.01) were upregulated in the RPS5 treatment group Conclusion: Feeding 5% RPS altered bacterial community composition and promoted gut health in weaned piglets. Thus, resistant starch as a feed additive may prevent diarrhea in piglets during weaning.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.25-25
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• 2022

Drought becomes frequent and severe because of continuous global warming, leading to a significant loss of crop yield. In soybean (Glycine max [L.]), most of quantitative trait loci (QTLs) analyses for drought tolerance have conducted by investigating yield changes under water-restricted conditions at the reproductive stages. More recently, the necessity of QTL studies to use physiological indices responding to drought at the early growth stages besides the reproductive ones has arisen due to the unpredictable and prevalent occurrence of drought throughout the soybean growing season. In this study, we thus identified QTLs conferring wilting scores and moisture contents of soybean subjected to drought stress in the early vegetative stage using an recombinant inbred line (RIL) population derived from a cross between Taekwang (drought-sensitive) and SS2-2 (drought-tolerant). For the two traits, the same major QTL was located on chromosome 10, accounting for up to 11.5% of phenotypic variance explained with LOD score of 12.5. This QTL overlaps with a reported QTL for the limited transpiration trait in soybean and harbors an ortholog of the Arabidopsis ABA and drought-induced RING-D UF1117 gene. Meanwhile, one of important features of plant drought tolerance is their ability to limit transpiration rates under high vapor pressure deficiency in response to mitigate water loss. However, monitoring their transpiration rates is time-consuming and laborious. Therefore, only a few population-level studies regarding transpiration rates under the drought condition have been reported so far. Via employing an Arduino-based platform, for the reasons addressed, we are measuring and recording total pot weights of soybean plants every hour from the 1^st day after water restriction to the days when the half of the RILs exhibited permanent tissue damage in at least one trifoliate. Gradual decrease in moisture of soil in pots as time passes refers increase in the severity of drought stress. By tracking changes in the total pot weights of soybean plants, we will infer transpiration rates of the mapping parents and their RILs according to different levels of VPD and drought stress. The profile of transpiration rates from different levels of severity in the stresses facilitates a better understanding of relationship between transpiration-related features, such as limited maximum transpiration rates, to water saving performances, as well as those to other drought-responsive phenotypes. Our findings will provide primary insights on drought tolerance mechanisms in soybean and useful resources for improvement of soybean varieties tolerant to drought stress.