Journal of odor and indoor environment (실내환경 및 냄새 학회지)

Korean Society for Indoor Environment (한국실내환경학회)
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Characterization of odor-associated fungal community in automobile HVAC systems using a high-throughput DNA sequencing method

고속 염기서열 분석법을 이용한 자동차 공조 시스템(HAVC systems)의 악취 연관 곰팡이 군집 특성

  • Lee, Yun-Yeong (Department of Environmental Science and Engineering, Ewha Womans University) ;
  • Choi, Hyungjoo (Department of Environmental Science and Engineering, Ewha Womans University) ;
  • Yun, Jeonghee (Department of Environmental Science and Engineering, Ewha Womans University) ;
  • Ryu, Hee Wook (Department of Chemical Engineering, Soongsil University) ;
  • Cho, Jong Rae (Research & Development Division, Hyundai Motor Group) ;
  • Seong, Kwangmo (Research & Development Division, Hyundai Motor Group) ;
  • Cho, Kyung-Suk (Department of Environmental Science and Engineering, Ewha Womans University)
  • 이윤영 (이화여자대학교 환경공학과) ;
  • 최형주 (이화여자대학교 환경공학과) ;
  • 윤정희 (이화여자대학교 환경공학과) ;
  • 류희욱 (숭실대학교 화학공학과) ;
  • 조종래 (현대자동차 연구개발본부) ;
  • 성광모 (현대자동차 연구개발본부) ;
  • 조경숙 (이화여자대학교 환경공학과)
  • Received : 2017.01.12
  • Accepted : 2017.03.07
  • Published : 2017.03.31
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Abstract

The Automobile HVAC system is a habitat for odor-associated fungal communities. We investigated the odor-associated fungal community in an automobile HVAC system using a high-throughput DNA sequencing method. The fungal community structure was evaluated via metagenome analysis. At the phylum level, Ascomycota and Basidiomycota were detected, accounting for 43.41% and 56.49% of the fungal community in the HVAC system, respectively. Columnosphaeria (8.31%), Didymella (5.60%), Davidiella (5.50%), Microxyphium (4.24%), unclassified Pleosporales (2.90%), and Cladosporium (2.79%) were abundant at phylum of Ascomycota and Christiansenia (36.72%), Rhodotorula (10.48%), and Sporidiobolus (2.34%) were abundant at phylum of Basidiomycota. A total of 22 genera of fungi were isolated and identified from the evaporators of the HVAC systems which support fungal growth and biofilm formation. Among them, Cladosporium, Penicillium, Aspergillus, and Alternaria are the most representative odor-associated fungi in HVAC systems. They were reported to form biofilm on the surface of HVAC systems with other bacteria by hypha. In addition, they produce various mVOCs such as 3-methyl-1-butanol, acetic acid, butanoic acid, and methyl isobutyl ketone. Our findings may be useful for extending the understanding of odor-associated fungal communities in automobile HVAC systems.

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