• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.74-74
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• 2003

In present study, attempts were made to preserve abalone (Haliotis discus hannai) sperm in liquid form at low temperature, to evaluate the effect of various diluents in short-term storage on sperm, and cryopreservation procedures were optimized for the cryoprotectants as well as freezing rates, in terms of the motility and survival rate, and the ultrastructural changes of sperm after short-term storage and cryopreservation were observed. The abalone sperm reached maximum motility until about 4min after activation. The motility was constant for about 16min, after which it dropped gradually, and about 50min later all motility ceased. Threshold activation of sperm was found in 40% artificial seawater (ASW), and motility increased as the concentration of ASW increased. In Hanks balanced salt solution without calcium (Ca-Free HBSS, 300 and 400 mOsmol/kg) and 10%, 20%, and 30% ASW the sperm was immotile, and motility once again restored incompletely only in HBSS of 300 and 400 mOsmol/kg, 20% and 30% ASW after 100% ASW was added. Sperm motility was extended following 20 days of cold storage only in 70% and 100% ASW. A high motility index of 3.5-4.5 was observed for the first 8 days in 70% and 80% ASW. In other diluents sperm motility was constant less than 10 days, and the motility index was obviously lower than that of sperm in 70% and 100% ASW. After 20 days of cold storage survival rates of 10.2%-20.7% were obtained in ASW and 300 mOsmol/kg HBSS, and that in 400 HBSS (65.3%) was significantly higher than others. The constant period of sperm motility stored in 70% ASW was longer obviously than that in 100% ASW after 6 days of storage, and the time to maximum motility of sperm stored in 70% increased gradually, while the difference in which of sperm in 100% ASW was not significant. The sperm plunged into liquid nitrogen all died except that sperm using 15% glycerol as cryoprotectant restored 10.4% of motility. The highest motility index (3.4) was obtained with 5% glycerol and freezing procedure: $50^{\circ}C$/min from $20^{\circ}C$ to $-80^{\circ}C$.

• Journal of Bio-Environment Control
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• v.17 no.3
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• pp.238-245
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• 2008

This study was carried out to investigate the effect of magnesium (Mg) concentrations in nutrient solution on growth of and nutrient uptake by 'Maehyang' strawberry. Tissue analysis based on dry weight and petiole sap were also conducted to determine the threshold level in plants when Mg deficiency disorders developed in strawberry plants. In the Mg deficient plants, the spotted yellowing or yellowing area developed on the interveinal area of mature leaves. The dark brown color also developed on the interveinal area of mature leaves with marginal browning or marginal necrosis. The response in dry weight production of 'Maehyang' strawberry to elevated Mg concentrations in nutrient solution was quadratic and the equation is y=6.84+1.7533x-$0.9278x^2$ $(R^2=0.1081^{***})$. But the Mg contents in tissue increased lineally with the equation of y=0.1764+0.1275x $(R^2=0.8307^{***})$. The trends of fresh weight production and Mg concentrations in petiole sap were also quadratic (y=24.127+7.3565x-$1.125x^2$, $R^2=0.2314^{^***}$) and linear (11.954+5.793x, $R^2=0.6869^{***}$), respectively. To prevent growth suppression, the Mg concentrations based on dry weight of above ground tissue and in petiole sap should be in the range of 0.30 to 0.65% and 19 to $40mg{\cdot}kg^{-1}$, respectively, fer the commercial production of 'Maehyang' strawberry.

• Tunnel and Underground Space
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• v.22 no.3
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• pp.163-172
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• 2012

As more diesel engines have been employed in underground limestone mines with large cross section, underground space environment is worsened by diesel exhausts and heat flow. This paper aims for the ultimate goal to optimize the work place environment through assuring the optimal required ventilation rate based on the analysis of the airflow, diesel exhaust gas concentrations and the effects of mechanization and deepening working face on temperature and humidity. Due to the insufficient capacity of the main exhaust fan and poor airway management, stagnant airflows were observed at various locations, while the flow direction was reversed instantly with passing diesel equipment and the flow reversal was also made by the seasonal variation of the outside surface weather. During the loading operation, CO concentration measurements were found to be frequently higher than the threshold limit of 50 ppm, and most of the $NO_2$ measurements during drilling and loading operations shows even more serious levels surpassing the permissible limit of 3 ppm. The actual ventilation quantity was considerably less than the required quantity estimated by the mine health and safety law, and this shortage problem was less serious in colder winter showing more effectiveness of the natural ventilation.

• Journal of fish pathology
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• v.4 no.2
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• pp.71-77
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• 1991

From August to October of 1991, a bacterial disease occurred in cultured flounder, of 0 age Paralichthys olivaceus, in Cheju-do. Typical symptoms of the diseased fish were the hemorrage of the opercle, discoloration of the body, exophthalmus and hemorrhage of the eyes. The causative organism isolated from diseased fish was identified as a $\beta$-hemolytic Streptococcus. This bacterium was not identical with any known Streptococcus species listed in Bergey's Manual of Determinative Bacteriology 8th edition. However there was a close similarity between this species and Streptococcus sp. isolated from flounder and reported by Tashio (1983). The isolated bacteria streptococcus sp. showed very high sensitivity to the SM and EM, however, it resisted to the high concentration of Penicillin. In the pathagenicity analysis, about $1.1{\times}10^9$, $1.1{\times}10^7$, $1.1{\times}10^5$, $1.1{\times}10^3$, $1.1{\times}10^1cells$/100g B.W of isolated bacteria were injected percutaneouly. The results showed that this inoculation size $1.1{\times}10^3cells$/100g B.W was the threshold for the induction of mortality in pathogenicity analysis.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.18 no.2
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• pp.217-225
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• 2020

Concrete waste accounts for approximately 70~80% of the total waste generated during the decommissioning of nuclear power plants (NPPs). Based upon the concentration of each radionuclide, the concrete waste from the decommissioning can be used in the determination of the clearance threshold used to classify waste as radioactive. To reduce the cost of radioactive concrete waste disposal, it is important to perform decontamination before self-disposal or limited recycling. Therefore, it is necessary to estimate the internal radioactivity distribution of radioactive concrete waste to ensure effective decontamination. In this study, the performance metrics of various Compton reconstruction algorithms were compared in order to identify the best strategy to estimate the internal radioactivity distribution in concrete waste during the decommissioning of NPPs. Four reconstruction algorithms, namely, simple back-projection, filtered back-projection, maximum likelihood expectation maximization (MLEM), and energy-deconvolution MLEM (E-MLEM) were used as Compton reconstruction algorithms. Subsequently, the results obtained by using these various reconstruction algorithms were compared with one another and evaluated, using quantitative evaluation methods. The MLEM and E-MLEM reconstruction algorithms exhibited the best performance in maintaining a high image resolution and signal-to-noise ratio (SNR), respectively. The results of this study demonstrate the feasibility of using Compton images in the estimation of the internal radioactive distribution of concrete during the decommissioning of NPPs.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.43 no.11 s.353
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• pp.1-7
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• 2006

3-dimensional(3-D) simulations of ideal double-gate bulk FinFET were performed extensively and the electrical characteristics. were analyzed. In 3-D device simulation, we changed gate length($L_g$), height($H_g$), and channel doping concentration($N_b$) to see the behaviors of the threshold voltage($V_{th}$), DIBL(drain induced barrier lowering), and SS(subthreshold swing) with source/drain junction depth($X_{jSDE}$). When the $H_g$ is changed from 30 nm to 45nm, the variation gives a little change in $V_{th}$(less than 20 mV). The DIBL and SS were degraded rapidly as the $X_{jSDE}$ is deeper than $H_g$ at low fin body doping($1{\times}10^{16}cm^{-3}{\sim}1{\times}10^{17}cm^{-3}$). By adopting local doping at ${\sim}10nm$ under the $H_g$, the degradation could be suppressed significantly. The local doping also alleviated $V_{th}$ lowering by the shallower $X_{jSDE}\;than\;H_g$ at low fin body doping.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.20 no.4
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• pp.159-168
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• 2015

Forty-four surface sediments from Doam Bay were analyzed for total organic carbon (TOC), total nitrogen (TN), total metal (Al, Fe, Mn, Cr, Cu, Ni, Pb, Zn) and further chemical partitioning of metals were carried out in some samples. The TOC (0.32~3.10%) and TN (0.03~0.26%) values of the samples were similar to those of other coastal area. The C/N ratios ranged from 7.9 to 11.9 with an average 9.3 which revealed that contribution of terrestrial organic matters was relatively rare. Contents of analysed metals showed a level lower than threshold effects level (TEL) in sediment quality guidelines. Based on the chemical speciation of metals, the lattice fractions were found in the order Cr > Cu > Ni > Zn > Pb > Mn, while Mn and Pb are the ratio of the non-lattice fractions accounted for more than 50%. The average baseline values were obtained relative cumulative frequency curves and linear regression analysis. The respective baseline concentrations for Cu, Ni, Pb, Zn, Cr and Mn were 11.8, 23.1, 26.8, 76.6, 56.7, 585 mg/kg, respectively. Based on geoaccumulation index ($I_{geo}$) with a baseline values of Mn showed that face the contamination phase from estuarine stations. However, in case of Zn and Pb, although there is no sign of contamination, it could be release from sediment when there is a change in the environment, which is caused from the high ratio of non-lattice fractions.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.25 no.2
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• pp.42-53
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• 2020

In this study, heavy metal in road-deposited sediments (RDS) and marine sediment around Gwangyang Bay area have been investigated to assess the pollution status of metals and to understand the environmental impact of RDS as a potential source of metal pollution. Zn concentration for <63 ㎛ size fraction was the highest (2,982 mg/kg), followed by Cr, Ni, Pb, Cu, As, Cd, and Hg. Metal concentrations in RDS increased with decreasing particle size and relatively higher concentrations were observed around the metal waste and recycling facilities. For particle size in RDS smaller than 125 ㎛, EF values indicated that Zn was very high enrichment and Cr, Cd, Pb were significant enrichment. The concentrations of metals in marine sediments were mostly below the TEL value of sediment quality guidelines of Korea. However, the Zn concentrations has increased by 30~40% compared to 2010 year. The amounts of Zn, Cd and Pb in less than 125 ㎛ fraction where heavy metals can be easily transported by stormwater runoff accounted for 54% of the total RDS. The study area was greatly affected by Zn pollution due to corrosion of Zn plating materials by traffic activity as well as artificial activities related to the container logistics at Gwangyang container terminal. The fine particles of RDS are not only easily resuspended by wind and vehicle movement, but are also transported to the surrounding environments by runoff. Therefore, further research is needed on the adverse effects on the environment and ecosystem.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.17 no.1
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• pp.9-15
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• 2012

Worldwide development of harmful algal blooms causes serious problem for public health and fisheries industries. To evaluate the algicidal impact on the harmful algae bloom species in aquatic ecosystems of coast, a new algicide thiazolidinedione derivative (TD49) were tentatively examined in the growth stages (i.e., lag, logarithmic and stationary phase) of rapidophyceae $Heterosigma$ $akashiwo$, $Chattonella$ $marina$ and $Chattonella$ sp..Three strains could easily destroy in the lag phase due to relatively weak cell walls than those of the logarithmic and stationary phase. It is thought that inoculation of TD49 substances into initial or developmental natural blooms with a threshold concentration ($2{\mu}M$) can maximize the algicidal activity. Also, bio-chemical assays revealed that the algicidal substances from all culture strains were likely to be extracellular substances because those cells have easily destroyed in cell walls. On the other hand, natural zooplankton communities were influenced within the exposure experiments of $2{\mu}M$, which is showed the maximum algcidal activity of tested organisms. These results indicate that although the TD49 substance is potential agents for the control of $H.$ $akashiwo$, $C.$ $marina$ and $Chattonella$ sp. in the enclosed eutrophic bay and coastal water, more detailed research of acute toxicity effect on high trophic organism in marine ecosystems need to be conducted.

• Korean Journal of Soil Science and Fertilizer
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• v.36 no.6
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• pp.399-406
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• 2003

The application of potassium above the optimum level may cause the inhibition of plant growth, fertilizer loss, and environmental pollution. Therefore, application rate of K fertilizer should be recommended on the basis of soil test. In order to determine critical K content in soils causing growth inhibition of vegetables, $1m^2-pot$ experiments with Chinese cabbage and radish were accomplished with various K-application rates. The threshold concentrations of exchangeable potassium causing the inhibition of plant growth were $0.96cmol_c\;kg^{-1}$ for Chinese cabbage in spring, and $1.28cmol_c\;kg^{-1}$ for radish in autumn. Above those concentration levels, the yields of them were decreased with the increase of potassium levels in soils. Germination rate of Chinese cabbage in spring decreased with increase of the electrical conductivity (EC) of soils due to application of potassium fertilizer. In the harvesting stage, the potassium contents of plant were increased with the increase of K application rate while plant uptake of nutrients was decreased at the K adjustment level of over $2.0cmol_c\;kg^{-1}$.