• KSBB Journal
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• v.20 no.4
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• pp.271-277
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• 2005

The polyunsaturated fatty acids (PUFAs) are mostly synthesized by the marine original microorganisms. In this study, the effect of concentrations of glucose, NaCl, and $MgSO_4{\cdot}7H_2O$ on both the growth and the production of lipid and Docosahexaenoic acid (DHA) was investigated using Thraustochytrium aureum ATCC 34304. $Y_{Lipid/X}\;and\;Y_{DHA/Lipid}$ increased according to the increase of initial glucose concentration until 15 g/L of glucose. The maximum values of $Y_{Lipid/X}\;and\;Y_{DHA/Lipid}$ were 0.18 g/g and 0.41 g/g respectively at 15 g/L of glucose. The biomass yield, $Y_{x/s}$, however, was constant as 0.378 g/g regardless of initial glucose concentrations in the range of 5 g/L to 25 g/L. The specific growth rate of T. aureum increased also with initial glucose concentration from 5 g/L and 15 g/L. The maximum value was $0.79\;day^{-1}$ at 15 g/L of glucose, and the growth rate decreased to $0.57\;day^{-1}$ and remained constant according to initial sugar concentration in the range of higher than 15 g/L of glucose. The concentration of NaCl was changed from 0 g/L to 48 g/L, in order to study the characteristics of the growth and the production of lipid and DHA according to NaCl concentration. The growth and the production of lipid as well as DHA stopped all at 0 g/L of NaCl and the maximum values of all the three variables occurred at 24 g/L. The effect of $MgSO_4{\cdot}7H_2O$ concentration was also investigated not to find the growth and the production of lipid and DHA at 0 g/L. However, the growth and the Production with $MgSO_4{\cdot}7H_2O$ concentration increased to reach the maximum values at 18 g/L of $MgSO_4{\cdot}7H_2O$.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.807-807
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• 2003

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.580-580
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• 2003

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.617-617
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• 2003

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.688-689
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• 2005

• Microbiology and Biotechnology Letters
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• v.33 no.1
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• pp.51-55
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• 2005

The effect of rotation speed and culture temperature on the growth of T. aureum and also the biosynthesis of lipid and DHA was investigated. The optimal temperature for the growth was $32^{\circ}C$, but the best temperature for the maximum production of lipid and DHA was $18^{\circ}C$ in the range of $4^{\circ}C\;to\;39^{\circ}C$. In the case that the temperature was higher than $39^{\circ}C$ and lower than $11^{\circ}C$, the growth was very slow and the production of DHA was also very low. However, the lipid content in the biomass became higher with decrease in the culture temperature. The rotation speed for the maximum growth was 100rpm. But the growth and the production of DHA were not affected by the change of rotation speed in the range of 100 to 250 rpm. When the rotation speed was 50 rpm, the growth as well as the production of DHA and lipid was greatly reduced.

• KSBB Journal
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• v.15 no.2
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• pp.195-200
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• 2000

Studies were made on the optimization of media to c비tivate Thraustochytrium aureum A TCC 34304 for the enhanc엉d p production of linoleic acid. The medium optimization was made with the artificial sea water medium. Yeast extract, sodium g glutamate, peptone and tryptone were considered as nitrogen source. The effect of $\infty$ncentration of nitrogen source as well a as initial glucose on the production of linoleic acid were investigated to optimize the media. The maximum yield of lipid was 0 0.302 mg/g cell mass when initial glucose $\infty$ncentration was 10 g/L and sodium glutamate was used as nitrogen source, a and the yield of linoleic acid to unit cell mass was also maximum to be 8 % in that case. The highest linoleic acid c concentration was obtained in the initial glucose concentration 30 g/L regardless of the kinds of nitrogen source and the | linoleic acid concentration was 0.208 g/L when peptone was supplemented to be 2 g/L.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.434-437
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• 2001

Shaking cultivation was carried out in 250 mL Erlenmeyel' flasks containing 60 mL of culture solution for 7 days. Artificial seawater was used as a basal culture medium. The cultivation was performed under the conditions that the temperature ranged from $4^{\circ}C$ to $32^{\circ}C$, the agitation speed 50 rpm to 200 rpm, and the sugar concentration 5 g/L to 35 g/L. The medium condition was cultured that sugar and nitrogen ratio 1.25 to 10. The biomass. the lipid content in biomass, and the DBA content of lipid were investigated according to the cultivation conditions. The lipid in biomass was distributed between 10 and 349'0, the DHA in lipid 34% to 43% of the lipid. and the biomass concentration 0.425 g/L to 4.50 g/L.