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Effect of Operating Factors on the Growth and DHA Biosynthesis of Thraustochytrium aureum ATCC 34304  

Cho Dae-Won (Department of Biological Engineering, Inha University)
Song Sang-Kue (Department of Biological Engineering, Inha University)
Kim Won-Ho (Department of Biological Engineering, Inha University)
Hur Byung-Ki (Department of Biological Engineering, Inha University)
Publication Information
Microbiology and Biotechnology Letters / v.33, no.1, 2005 , pp. 51-55 More about this Journal
Abstract
The effect of rotation speed and culture temperature on the growth of T. aureum and also the biosynthesis of lipid and DHA was investigated. The optimal temperature for the growth was $32^{\circ}C$, but the best temperature for the maximum production of lipid and DHA was $18^{\circ}C$ in the range of $4^{\circ}C\;to\;39^{\circ}C$. In the case that the temperature was higher than $39^{\circ}C$ and lower than $11^{\circ}C$, the growth was very slow and the production of DHA was also very low. However, the lipid content in the biomass became higher with decrease in the culture temperature. The rotation speed for the maximum growth was 100rpm. But the growth and the production of DHA were not affected by the change of rotation speed in the range of 100 to 250 rpm. When the rotation speed was 50 rpm, the growth as well as the production of DHA and lipid was greatly reduced.
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