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Effects of Dietary Supplementation of Copper-Sulfate and Copper-Soy Proteinate on the Performance and Small Intestinal Microflora in Laying Hens (Copper-Sulfate와 Copper-Soy Proteinate 첨가가 산란계의 생산성과 장내 미생물 균총에 미치는 영향)

  • Kim, Chan Ho;Kang, Hwan Ku;Bang, Han Tae;Kim, Ji Hyuk;Hwangbo, Jong;Choi, Hee Cheol;Paik, In Kee;Moon, Hong Kil
    • Korean Journal of Poultry Science
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    • v.41 no.4
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    • pp.241-247
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    • 2014
  • The objective of this experimental was to investigate the effect of dietary Cu-sulfate and Cu-soy proteinate on productive performance and small intestinal microflora. A total 1,000 Hy-Line Brown laying hens (35 weeks old) were randomly allotted to 1 of 5 dietary treatments: Control, Cu-sulfate 50, 100 (50, 100 ppm Cu supplementation as Cu-sulfate) and Cu-SP 50, 100 (50, 100 ppm Cu supplementation as Cu-soy proteinate). Each treatment was replicated 4 times with fifty birds per replication, housed in 2 birds cages. Fifty birds units were arranged according to randomized block design. Feeding trial lasted 5 weeks under 16L : 8D lighting regimen. Hen day egg production was significantly (P<0.05) higher in Cu treated groups than control. Feed intake, broken and shell-less egg production was not significantly influenced by treatment. Eggshell color, eggyolk color, haugh unit, and eggshell thickness were not significantly influenced by treatment. However, eggshell strength was significantly (P<0.05) greater in Cu treated groups than control. Concentration of copper of liver was significantly (P<0.05) greater in Cu treated groups than control. Concentration of zinc and iron of liver were not influenced by treatments. Population of Cl. perfrigens and Lactobacilli in the small intestinal content were significantly (P<0.05) influenced by treatments. Population of Cl. perfrigens decreased and that of Lactobacilli increased in the Cu supplement groups. In conclusion, dietary Cu sulfate and Cu-soy proteinate similarly improves egg production, eggshell strength, and favors intestinal microbial population of laying hens.

Effects of Dietary Supplementation of Copper Soy Proteinate (Cu-SP) and Herbal Mixture (HBM) on the Performance, Blood Parameter and Immune Response in Laying Hens (Copper Soy Proteinate(Cu-SP)와 Herbal Mixture(HBM)의 급여가 산란계의 생산성, 혈액성상 및 면역체계에 미치는 영향)

  • Kim, Chan Ho;Kang, Hwan Ku;Bang, Han Tae;Kim, Ji Hyuk;Hwangbo, Jong;Choi, Hee Cheol;Paik, In Kee;Moon, Hong Kil
    • Korean Journal of Poultry Science
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    • v.41 no.4
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    • pp.323-329
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    • 2014
  • The objective of this experiment was to investigate the effect of dietary supplementation of copper-soy proteinate (Cu-SP) and herbal mixture (HBM) on growth performance, blood parameter, and immune response in laying hens. A total 800 Hy-Line Brown laying hens (60 weeks old) were randomly allotted to 1 of 4 dietary treatments : (1) Control : control diet, (2) Cu-SP : control diet + 100 mg/kg Cu-soy proteinate, (3) HBM : control diet + 0.15% herbal mixture, and (4) Cu-SP + HBM : control diet + 100 mg/kg Cu-soy proteinate + 0.15% herbal mixture. Each treatment was replicated 5 times with forty birds units were arranged according to randomized block design. Feeding trial lasted 5 weeks under 16L : 8D lighting regimen. The diet and water were available ad libitum. Result indicated that during feeding trial of the experiment, hen-day egg production was significantly (P<0.05) higher in Cu-SP and HBM treated groups than control. However, feed intake, feed conversion ratio, egg weight, broken and shell less egg production were not significantly influenced by treatments. Eggshell strength was significantly (P<0.05) higher in Cu-SP than control. Eggshell thickness, eggshell color, egg yolk color, Haugh unit were not significantly influenced by treatments. The level of WBC and stress index (heterophil : lymphocyte) were higher in supplemented groups than the control. The concentration of plasma IgG was higher in supplemented groups than the control. The result of this experiment showed that dietary copper-soy proteinate or herbal mixture tended to improve egg production and affect positively on immune response of laying hens.

The Dietary Effects of Marigold Extracts on Egg Production, Egg Quality and the Production of Lutein Fortified Chicken Eggs (사료 내 매리골드 추출물의 첨가 급여가 계란 생산성과 계란 품질 및 난황 내 루테인 함량에 미치는 영향)

  • Kim, Eun-Jib
    • Korean Journal of Poultry Science
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    • v.41 no.2
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    • pp.135-142
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    • 2014
  • This study was conducted to evaluate dietary effects of Marigold extract on laying performance, egg quality, oxidative stability of egg yolk and lutein transfer into chicken eggs. A total of one-hundred eighty nine 55-wk-old Hy-Line Brown layers were divided into seven groups and fed control diet or each experimental diet containing 0.1, 0.3, 0.5, 1.0, 1.5 or 2.0% Marigold extract. Egg production, egg weight and daily egg mass were not affected by dietary treatments. The yolk colors in groups fed diets containing Marigold extract were significantly higher than that of control. The Haugh unit were tended to be improved by feeding of diets containing Marigold extract although there were no significant difference in egg shell strength and thickness. The MDA (malondialdehyde) contents in groups fed diets containing Marigold extract above 0.5% were significantly reduced than that of control. After 14d of storage, the Haugh unit values in groups feed diets containing 0.3 and 1.0% Marigold extract were significantly higher than that of control (p<0.05). The concentration of lutein in egg yolk increased by feeding of Marigold extract. When 2% Marigold extract was supplemented to the diet, lutein content of egg was increased as much as 1.71 mg/60 g. These results indicated that the use of Marigold extract in layer diets was effective in egg quality and for the production of lutein fortified eggs.

Effects of Dietary Germanium on the Performance, Egg Quality and Blood Composition for the Finishing Stage of Laying Hens (산란말기에 게르마늄의 첨가 급여가 산란계의 생산성과 계란품질 및 혈액성상에 미치는 영향)

  • Lim, Chun Ik;Moon, Hong Kil;Kim, Sang Ho;Choe, Ho Sung;Ryu, Kyeong Seon
    • Korean Journal of Poultry Science
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    • v.45 no.2
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    • pp.119-124
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    • 2018
  • This study was conducted to assess the effect of dietary addition of Germanium (Ge) on performance, egg quality, serum biochemical properties and yolk fatty acid composition in laying hens. At 74 weeks of age, a total of 360 Hy-line brown commercial layers were divided into four treatments with five replicates per treatment. Four dietary levels of Ge (0, 1, 2 and 4%) were added to the basal diets (ME: 2,750 kcal/kg, CP: 16%) and fed corresponding diet for a period of 12 weeks. The effects of these treatments on production performance and egg quality were measured three times at four week intervals, and at the end of the experiment, blood and egg yolk fatty acid were analyzed. The results showed that production performance parameters including egg production rate, feed intake, egg weight, egg mass and feed conversion ratio were non-significant, but dietary addition of 2% Ge tended to increase egg production rate. Eggshell strength was found to be significantly higher in Ge supplemented groups than in the control group (P<0.05), and eggshell thickness was also trended to be improved in Ge treated groups. In serum, triglyceride content was significantly greater (P<0.05), and the highest total cholesterol was found in hens of the control group as compared with that of the Ge supplemented groups. The yolk fatty acid composition including polyunsaturated fatty acid, unsaturated fatty acid and saturated fatty acid were not affected among the treatments but addition of 2 or 4% Ge in basal diet had significantly (P<0.05) increased monounsaturated fatty acid. Therefore, supplementation of Ge in laying hens diet has positive effects on egg quality and triglyceride in serum.

The Study on the Reduction of Patient Surface Dose Through the use of Copper Filter in a Digital Chest Radiography (디지털 흉부 촬영에서 구리필터사용에 따른 환자 표면선량 감소효과에 관한 연구)

  • Shin, Soo-In;Kim, Chong-Yeal;Kim, Sung-Chul
    • Journal of radiological science and technology
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    • v.31 no.3
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    • pp.223-228
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    • 2008
  • The most critical point in the medical use of radiation is to minimize the patient's entrance dose while maintaining the diagnostic function. Low-energy photons (long wave X-ray) among diagnostic X-rays are unnecessary because they are mostly absorbed and contribute the increase of patient's entrance dose. The most effective method to eliminate the low-energy photons is to use the filtering plate. The experiments were performed by observing the image quality. The skin entrance dose was 0.3 mmCu (copper) filter. A total of 80 images were prepared as two sets of 40 cuts. In the first set (of 40 cuts), 20 cuts were prepared for the non-filter set and another 20 cuts for the Cu filter of signal + noise image set. In the second set of 40 cuts, 20 cuts were prepared for the non-filter set and another 20 cuts for the Cu filter of non-signal image (noisy image) with random location of diameter 4 mm and 3 mm thickness of acryl disc for ROC signal at the chest phantom. P(S/s) and P(S/n) were calculated and the ROC curve was described in terms of sensitivity and specificity. Accuracy were evaluated after reading by five radiologists. The number of optically observable lesions was counted through ANSI chest phantom and contrast-detail phantom by recommendation of AAPM when non-filter or Cu filter was used, and the skin entrance dose was also measured for both conditions. As the result of the study, when the Cu filter was applied, favorable outcomes were observed on, the ROC Curve was located on the upper left area, sensitivity, accuracy and the number of CD phantom lesions were reasonable. Furthermore, if skin entrance dose was reduced, the use of additional filtration may be required to be considered in many other cases.

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Growth, Reproduction, Mortality, and Production of Laminaria japonica Areschoug on the Southeastern Coast of Korea (한국 동남해안에서 다시마(Laminaria japonica Areschoug)의 성장, 성숙, 사망률 및 생산)

  • Kang, Rae-Seon;Koh, Chul-Hwan
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.4 no.3
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    • pp.226-236
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    • 1999
  • Growth, reproduction, mortality, and production of Laminaria japonica were experimentally studied at a cultivation ground on the coast of llkwang, where the largest amount of cultivated Laminaria has been produced in Korea. For this experiment, young sporophytes (0.33 cm in mean length) grown in the laboratory were transplanted at the depth of 3 m and field surveys on them were conducted twice a month from December, 1995 to August, 1996. Plants exhibited an annual life span; they were completely dead by August. Frond width, thickness, and wet weight showed similar pattern of seasonal growth and reached their maxima in July, but frond length showed no more increment after May. Maximum mean frond length and weight were 199.8 cm and 333.0 g wet wt., respectively. Overall meristematic growth in length and weight were 384.0 cm and 393.6 g wet wt., respectively. Absolute growth rates (AGR) which were calculated from the length of tissue developed from meristem varied seasonally; AGR of length and weight reached maxima in March (3.6 $cm{\cdot}d^{-1}$) and May (3.8 g wet $wt{\cdot}d^{-1}$), respectively. Absolute attrition rates gradually increased from February to July. Seasonal differences in growth and attrition rates appeared to be related to seawater temperature and nitrogen concentration in seawater. Reproductive sporophytes bearing sprorangium sorus began to occur from April, and the ratio of sorus area to blade area reached its maximum in July (0.034). Survival rate was exponentially decreased; more than 90% of plants decayed within 56 days after outplanting. After February, mortality was size-specific; mortality of smaller plants less than 30 cm in length were relatively higher. Maximum biomass occured in July (285.6 kg wet $wt{\cdot}m^{-2}$) and annual production was 758.7 kg wet $wt{\cdot}m^{-2}$.

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Comparison of Electrical Signal Properties about Top Electrode Size on Photoconductor Film (광도전체 필름 상부 전극크기에 따른 전기적 신호 특성 비교)

  • Kang, Sang-Sik;Jung, Bong-Jae;Noh, Si-Cheul;Cho, Chang-Hoon;Yoon, Ju-Sun;Jeon, Sung-Pyo;Park, Ji-Koon
    • Journal of the Korean Society of Radiology
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    • v.5 no.2
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    • pp.93-96
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    • 2011
  • Currently, the development of direct conversion radiation detector using photoconductor materials is progressing in widely. Among of theses photoconductor materials, mercuric iodide compound than amorphous selenium has excellent absorption and sensitivity of high energy radiation. Also, the detection efficiency of signal generated in photoconductor film varies by electric filed and geometric distribution according to top-bottom electrode size. Therefore, in this work, the x-ray detection characteristics are investigated about the size of top electrode in $HgI_2$ photoconductor film. For sample fabrication, to solve the problem that is difficult to make a large area film, we used the spatial paste screen-print method. And the sample thickness is $150{\mu}m$ and an film area size is $3cm{\times}3cm$ on ITO-coated glass substrate. ITO(Indium-Tin-Oxide) electrode was used as top electrode using a magnetron sputtering system and each area is $3cm{\times}3cm$, $2cm{\times}2cm$ and $1cm{\times}1cm$. From experimental measurement, the dark current, sensitivity and SNR of the $HgI_2$ film are obtained from I-V test. From the experimental results, it shows that the sensitivity increases in accordance with the area of the electrode but the SNR is decreased because of the high dark current. Therefore, the optimized size of electrode is importance for the development of photoconductor based x-ray imaging detector.

Reliability Verification of FLUKA Transport Code for Double Layered X-ray Protective Sheet Design (이중 구조의 X선 차폐시트 설계를 위한 FLUKA 수송코드의 신뢰성 검증)

  • Kang, Sang Sik;Heo, Seung Wook;Choi, Il Hong;Jun, Jae Hoon;Yang, Sung Woo;Kim, Kyo Tae;Heo, Ye Ji;Park, Ji Koon
    • Journal of the Korean Society of Radiology
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    • v.11 no.7
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    • pp.547-553
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    • 2017
  • In the current medical field, lead is widely used as a radiation shield. However, the lead weight is very heavy, so wearing protective clothing such as apron is difficult to wear for long periods of time and there is a problem with the danger of lethal toxicity in humans. Recently, many studies have been conducted to develop substitute materials of lead to resolve these problems. As a substitute materials for lead, barium(Ba) and iodine(I) have excellent shielding ability. But, It has characteristics emitting characteristic X-rays from the energy area near 30 keV. For patients or radiation workers, shielding materials is often made into contact with the human body. Therefore, the characteristic X-rays generated by the shielding material are directly exposured in the human body, which increases the risk of increasing radiation absorbed dose. In this study, we have developed the FLUKA transport code, one of the most suitable elements of radiation transport codes, to remove the characteristic X-rays generated by barium or iodine. We have verified the reliability of the shielding fraction of the structure of the structure shielding by comparing with the MCPDX simulations conducted as a prior study. Using the MCNPX and FLUKA, the double layer shielding structures with the various thickness combination consisting of barium sulphate ($BaSO_4$) and bismuth oxide($Bi_2O_3$) are designed. The accuracy of the type shown in IEC 61331-1 was geometrically identical to the simulation. In addition, the transmission spectrum and absorbed dose of the shielding material for the successive x-rays of 120 kVp spectra were compared with lead. In results, $0.3mm-BaSO_4/0.3mm-Bi_2O_3$ and $0.1mm-BaSO_4/0.5mm-Bi_2O_3$ structures have been absorbed in both 33 keV and 37 keV characteristic X-rays. In addition, for high-energy X-rays greater than 90 keV, the shielding efficiency was shown close to lead. Also, the transport code of the FLUKA's photon transport code was showed cut-off on low-energy X-rays(below 33keV) and is limited to computerized X-rays of the low-energy X-rays. But, In high-energy areas above 40 keV, the relative error with MCNPX was found to be highly reliable within 6 %.

Effect of Nandrolone Decanoate on Disuse Muscle Atrophy and Bone Beating in Dogs (개에서 불용성 근위축과 골절 치유에 대한 Nandrolone decanoate의 효과)

  • Yun Seong-jin;Lim Ji-hey;Rahman Md. Mizanur;Byeon Ye-eun;Kim Wan-hee;Kweon Oh-kyeong
    • Journal of Veterinary Clinics
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    • v.22 no.4
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    • pp.336-341
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    • 2005
  • Anabolic steroid compounds are widely used for the increase of muscle mass, density of bone and athletic ability. The present study conducted to evaluate the effects of nandrolone decanoate (ND), one of the anabolic steroid compounds, on disuse muscle atrophy and healing process of bone in dogs. Twenty physically healthy dogs of both sexes were used in this experiment and divided into three groups: group A (control), group B (low dose M-1.5 mg/kg) and group C (high dose ND-7.5 mg/kg). One-mm strip of full thickness bone was excised from the radius below the pronator teres muscle for the artificial fracture and then the fractured ends were fixed in apposition with bone plate leaving 1mm gap, and finally immobilized externally by Robert John's bandage for 4 weeks. ND was administered intramuscularly once a week far 8 weeks. Body weight, muscle mass change and fracture gap of the bone were evaluated immediately after surgery, and 2, 4, and 8 weeks after surgery. The rates of muscle mass change 8 weeks after surgery were $-2.75\pm0.16\%,\;1.68\pm0.11\%\;and\;1.74\pm0.48\%$ in groups A, B and C, respectively. The significant increase (p<0.05) of muscle mass increments were found in the treated groups. The fibrous connective tissue layer in the fracture gaps of the treated groups increased more than the control, especially in the group C at 4th week. More dense fibrous connective tissue were found in the treated groups at 8th week. Collectively, our results suggested that ND was an effective anabolic agent for the immobilized disuse muscle and bone healing.

Study on a pair of small spot of nonsexual gland hind of the larvae in Bombyx mori L. (I) (유충의 생식후원선이 아닌 한 쌍의 소반점에 관한 조사연구 (1))

  • 윤종관
    • Journal of Sericultural and Entomological Science
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    • no.11
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    • pp.51-54
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    • 1970
  • External features of silkworms are not evident during stage of larvae and pupae like in the stage of imagines. In general, therefore, sexing of larvae has been depended on Herold's gland of males or Ishiwata' sexual spots (Anterior sexual spots and posterior sexual spots) of a female 2-3 days after of the 5th age since it is relatively easy to classify in this period. Sometimes. however, we made mistake of sexing since a pair of small spot around the abdomen of 9th abdominal of males and posterior sexual spot of females was found at the same position. This study has been conducted in order to obtain essential points of classifing the above it will be summarized as follows: A. Existence of the males small spots on 9th abdominal segment were varied by races. Through observation races by races the more larvae which had the small spots were found in chinese races than the Japanese, and 57 percent of the Chinese orgin larvae of the small spot were picked among total counted males. B. The small spots were more or less roundly shaped just as posterior sexual spots of females, and these spots were colored slightly even the colors were somewhat different by races. Milky color was seen in the case of the posterior sexual spots. The small spots were observed smaller than the posterior sexual spots. It is difficult classify the sexual spots during stage of mature larvae, but it was easily classified small spots by the naked eye even in the same stage. The small spots were found at the same site of the posterior sexual spots. C. After sexing by based on the small spots and Herold's gland, the sexing through purpae bodies were carried out again to confirm its correctness. Average 5. 2 percent of more opposite sex were found in the latter. This figures supposed to be caused the location of the small spots coincide with the posterior sexual spots. D. The moths, no disorder were found in daily activities and their generative functions depend on the existence of the small spot and thickness of its color. E. It is presumed that existence of the small spots were caused by races, heredity nature, voltinism, moulting, and etc.

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