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Effects of Artificial Light Sources on Growth and Yield of Peucedanum japonicum Hydroponically Grown in Plant Factory (식물공장 인공광원이 방풍나물의 생육 및 수량에 미치는 영향)

  • Lee, Guang-Jae;Heo, Jeong-Wook;Kim, Hyun-Hwan;Jung, Chung-Ryul;Kim, Dong-Eok;Nam, Sang-Young
    • Journal of Bio-Environment Control
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    • v.25 no.1
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    • pp.16-23
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    • 2016
  • This study was carried out to investigate the effects of artificial light sources on growth and yield of hydroponically grown Peucedanum japonicum in plant factory. Treatments were composed with; florescent lamp(FL) as control, and LED lights; R:B(2:1, RB), R:B:W(2:1:3, RBW), and R:B:G:W(2:1:0.5:3, RBGW). Plant height of RBGW and FL treatments were superior to RB and RBW. Leaf weight of RBW and RB were superior to FL and RBGW. There were no significant difference of leaf length and thickness among the treatments. Lightness of leaves was same tendency with plant height. Total phenolic compound content was the high in order of RB as $105.77mg{\cdot}100g^{-1}$ GE, RBW as $92.52mg{\cdot}100g^{-1}$ GE, FL as $89.08mg{\cdot}100g^{-1}$ GE, and RBGW $82.00mg{\cdot}100g^{-1}$ GE. Total flavonoids were not detected in all treatments. Vitamin C content was the highest in RB and the lowest in FL. Total dietary fiber were the highest in FL and the lowest in RBGW. There was no significant difference cystein and methionine contents among the treatments. Concludely, yield, total phenolic compounds, and vitamin C content was high in RBW and RB. We reached conclusion that RBW is best artificial light source considering yield, functionality and eye fatigability when work. We recommend to further study LED pulse and duty rates for increasing functionality.

Effect of Transplanting Methods on Growth and Yield of Paprika in Coir Culture (코이어 배지 수경재배에서 정식방법이 파프리카 생육과 수량에 미치는 영향)

  • Kim, Cho Hee;Lee, Change Hee;Kweon, Oh Yeol;An, Chul Geon
    • Journal of Bio-Environment Control
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    • v.23 no.4
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    • pp.281-287
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    • 2014
  • This study was carried out to investigate the effect of transplanting methods on the growth and yield of paprika (Capsicumannuum L. 'Veyron' and 'Coletti') in coir culture during two seasons. The summer type sowed in late winter and harvested from summer and the winter type sowed in summer and harvested from early winter. Control plants grown on the 10cm rockwool block were transplanted on coir slab when the 8 leaves of seedlings were emerged, while plants of the young seedling transplanting(YST) grown on the 7cm rockwool block was put on the slab at time of 2-3 leaves developed. Plants of the temporary transplanting(TT) on the 10cm rockwool block were moved on the slab after 2-3 weeks underpinning cultivation, while plants of the blockless transplanting(BT) were directly transplanted in the slab when the seedlings have 2-3 leaves emerged. The plant height of the control and BT treatment were longer while that of TT showed the shortest among treatments. The bigger leaf size was observed in the YST and BT treatment. Leaf number of the BT treatment was increased, while that of TT was the lowest. There were no differences in fruit size, locules and thickness among treatments. The lower fruit weight was observed in the TT of the winter culture and fruits in the control and YST of the summer culture showed higher fruit weight. The percentage of marketable fruit appeared to be slightly higher in the winter culture than in the summer culture. There were no differences in marketable fruit rate among the treatments of the winter culture but, among the summer culture, the highest marketable fruit rate was observed in the BT with 93%. The yield of the YST and BT was higher and that of the TT was the lowest.

Establishment of Optimum Nitrogen and Potassium Application for Paprika Fertigation (파프리카 관비재배를 위한 질소 및 칼륨의 시비량 설정)

  • Choi, Gyeong Lee;Rhee, Han Cheol;Yeo, Kyung Hwan;Lee, Seong Chan;Kang, Nam Jun;Choi, Hyo Gil
    • Journal of Bio-Environment Control
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    • v.26 no.1
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    • pp.1-6
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    • 2017
  • The paprika has emerged as one of the highest-income crops by increase in dimestic and export demand in the greenhouse crops. Nevertheless, there is no standard for fertigation in soil, because general culture system is soilless culture. This study was conducted to establish the optimum nitrogen and potassium application level for paprika fertigation. Four different levels of nitrogen and potassium were applied, treatment levels were 0.5, 1.0, 1.5, 2.0 times of pimiento fertilization recommendations based on soil testing. Experiment to instigate the optimum amounts of nitrogen and potassium were carried out in 2012 and 2013, respectively13. Nitrogen application : stem diameter of 0.5 times was significantly lower than other treatments, but stem length was not affected by nitrogen fiertigation levels. Number of fruit and yield of first fruiting group harvest were not significant difference. but those of the second fruiting group were decreased by increasing nitrogen level beyond 1.0 times treatment and were the lowest in 0.5 times treatment. Overall, the optimum level of nitrogen for fertigation was judged 1.0 times of pimiento fertilization recommendations based on soil testing. Potassium application : Growth was no signigicant trend except stem length. Number of locule, fresh thickness and sugar content were not significant difference. Number of fruit and yield were not significant difference at the first and second fruiting group harvest. But those were significant difference at third fruiting group harvest, maximum yield was obtained by 1.5 times fertigation level. The optimum level of potassium for fertigation was judged 1.5 times of pimiento fertilization recommendations based on soil testing.

The Clinical Effect and Construction of a Stereotactic Whole Body Immobilization Device (전신 정위 고정장치 제작과 임상효과에 대한 연구)

  • 정진범;정원균;서태석;최경식;진호상;지영훈
    • Progress in Medical Physics
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    • v.15 no.1
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    • pp.30-38
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    • 2004
  • Purpose: To develop a whole body frame for the purpose of reducing patient motion and minimizing setup error for extra-cranial stereotactic radiotherapy, and to evaluate the repositioning setup error of a patient in the frame. Materials and Methods: The developed whole body frame is composed of a base plate, immobilizer, vacuum cushion, ruler and belts. The dimension of the base plate is 130 cm in length, 50 cm in width and 1 cm in thickness. The material used in the base plate of the frame was bakelite and the immobilizer was made of acetal. In addition, Radiopaque angio-catheter wires were engraved on the base plate for a coordinate system to determine the target localization. The measurement for radiation transmission and target localization is peformed in order to test the utilization of the frame. Also, a Matlab program analyzed the patients setup error by using the patient's setup images obtained from a CCTV camera and digital record recorder (DVR). Results: A frame that is useful for CT simulation and radiation treatment was fabricated. The frame structure was designed to minimize collisions from the changes in the rotation angle of the gantry and to maximize the transmission rate of the Incident radiation at the lateral or posterior oblique direction. The lightening belts may be used for the further reduction of the patient motion, and the belts can be adjusted so that they are not in the way of beam direction. The radiation transmission rates of this frame were measured as 95% and 96% at 10 and 21 MV, respectively. The position of a test target on the skin of a volunteer is accurately determined by CT simulation using the coordinate system in the frame. The estimated setup errors by Matlab program are shown 3.69$\pm$1.60, 2.14$\pm$0.78 mm at the lateral and central chest, and 7.11 $\pm$2.10, 6.54$\pm$2.22 mm at lateral and central abdomen, respectively. The setup error due to the lateral motion of breast is shown as 6.33$\pm$ 1.55 mm. Conclusion: The development and test of a whole body frame has proven very useful and practical in the radiosurgery for extra-cranial cancers. It may be used in determining target localization, and it can be used as a patient immobilization tool. More experimental data should be obtained in order to improve and confirm the results of the patient setup error.

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Studies on the Processing of Powdered Katsuobushi and Its Flavor Constituents 1. Processing Conditions of Powdered Katsuobushi and Its Taste Compounds (분말가쓰오부시의 제조 및 풍미성분에 관한 연구 1. 분미가쓰오부시의 가공조건 및 정미성분)

  • OH Kwang-Soo;LEE Eung-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.21 no.1
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    • pp.21-29
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    • 1988
  • This study was carried out to develop the powdered Katsuobushi (a kind of boiled, smoked, and dried fish product which is used for seasoning soup as it is.) using skipjack as a natural flavoring substance. The processing conditions of the powdered Katsuobushi and the changes of taste compounds during processing of the products were examined. In preparation of the powdered Katsuobushl, frozen skipjack was thawed, beheaded, gutted, filleted and then sliced to 1cm of thickness. The silted meats were boiled in skipjack extract for 20 minutes, and then it was smoked for, 3 times to $10\~12\%$ moisture content at $80^{\circ}C$ for 8 hours. The smoked - dried meats were followed to be 50 mesh of particle size. The effect of slicing and boiling in skipjack meat extract on enhancing flavor and on preventing lipid oxidation of product during processing were observed. The moisture content and crude lipid content of the powdered Katsuobushi was in the range of 11 to $12\%$ and 4.3 to $4.8\%$, respectively. The taste compounds of the product were nucleotides and their related compounds, 1135.8mg/100g ; free amino acid and related compounds, 2210.2mg/100g ; non-volatile organic acids, 1148.0mg/100g ; and total creatinine. 592.1mg/100g on dry basis, and small amount of betaine and TMAO. The major elements of mineral in the product were found to be K, Mg, Na, and Ca. The content of IMP was 542.0mg/100g, and major free amino acids were found to be histidine, anserine, taurine, carnosine and alanine of which occupied to $83.6\%$ of total free amino acids. In non -volatile organic acids, major ones were lactic acid, succinic acid, pyroglutamic acid and $\alpha-ketoglutaric$ acid. From the results of the chemical experiments and sensory evaluation, we may conclude that the flavor of the product from present experiment is more desirable than that of conventional products although the processing time used were much shortened than that of conventional method, and it can be commercialized as a seasoning powder.

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Fundamental Studies for the Breeding of Marine Algae 3. Effects of Plant Hormones on the Growth of Porphyra-fronds (해조의 육종을 위한 기초적 연구 3. 김 엽체에 미치는 식물호르몬의 생장효과)

  • KIM Joong-Rae;JO Young-Won;HAN Chang-Yeal;LEE Man-Sang
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.14 no.2
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    • pp.79-85
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    • 1981
  • As one of the fundamental studies for the breeding of marine algae, the effects of several plant hormones (IAA, Gibberellin, 2.4-D, NAA, Kinetin) on the growth of Porphyra-fronds, P. tenera Kjell. form tamatsuensis Miura, were investigated from January 21 to February 21 1981. The fronds used for the experiment were dissected out at $25mm^2$ size, and cultured in modified Provasoli's ESP medium supplemented with various concentrations of each plant growth regulators. The culture was kept under constant water temperature of $5^{\circ}C$ in 14 hrs. photoperiod and illuminated with 2,400 lux by fluorescent light. Based on the results of first experiment, the culture of fronds for the secondary experiment was carried out at $5^{\circ}C\;and\;10^{\circ}C$ in medium containing various levels of Kinetin from April 6 to 24, and compared the growth of two groups at each concentrations with each other, The results obtained are summarized as follows : (1) The best growth efficiencies were observed at 5.0mg/1 of each plant hormones except Gibberellin. Among them, the highest growth-rate was $312.5\%\;(345.3\%\;in\;frond\;size)$ in contrast with control at 5.0mg/1 of Kinetin, and was followed by $257.5\%\;(236.1\%)$ in 2.4-D,$166.7\%(147.6\%)$ in IAA and $141.7\%\;(167.7\%)$ in NAA, but that in Gibberellin was $247.9\%(241.9\%)$ at 10.0mg/l. (2) Especially, the fronds cultured at 5.0mg/1 of Kinetin were deep black-brown in colour, and had vivid, healthy chloroplasts in their all cells. On the contrary, the fronds cultured in other media were discoloured to light black-brown or green-drown, and almost all cells were vacuolated or shrunk gradually into death.(3) There was an obvious difference between the best growth-rates of the fronds cultured at 5.0mg/l of Kinetin at $5^{\circ}C$ and those at $10^{\circ}C$. The former was $366.7\%$, the latter $318.8\%$ but the difference was little at lower concentrations. (4) Many abnormal cells grown up to $25.0-27.5\mu$ in diameter were found among the marginal cells of fronds which showed the best growth in Kinetin, and the fronds wire $41.0-42.0\mu$ in thickness which was thicker by $10.0\mu$ or so than the others. (5) In two fronds at 1.0mg/1 of Kinetin cell-divisions were observed, which might developed into antheridium, but it was doubtful whether due to the efficiency of Kinetin.

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Left ventricular dysfunction measured by tissue Doppler imaging and strain rate imaging in hypertensive adolescents (고혈압 청소년에서 tissue Doppler imaging과 strain rate imaging을 이용한 좌심실 기능 이상에 대한 연구)

  • Ahn, Hye Mi;Jung, Sun Ok;Kwon, Jung Hyun;Hong, Young Mi
    • Clinical and Experimental Pediatrics
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    • v.53 no.1
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    • pp.72-79
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    • 2010
  • Purpose: Left ventricular (LV) hypertrophy and impaired diastolic function may occur early in systemic hypertension. Diastolic dysfunction is associated with increased cardiovascular risk. Tissue Doppler imaging (TDI)-derived tissue velocity and strain rate are new parameters for assessing diastolic dysfunction. The aim of this study is to determine whether TDI and strain rate imaging (SRI) would improve the ability to recognize early impaired diastolic and systolic functions compared with conventional echocardiography in hypertensive adolescents. Methods: We included 38 hypertensive patients with systolic blood pressure above 140 mmHg or diastolic blood pressure above 90 mmHg. Ejection fraction and myocardial performance index (MPI) were estimated by conventional echocardiography. Peak systolic myocardial velocity, early diastolic myocardial velocity (Em), and peak late diastolic myocardial velocity (Am) were obtained by using TDI and SRI. Results: In the hypertensive group, interventricular septal thickness was significantly increased on M-mode echocardiography. Em/Am was significantly decreased at the mitral valve annulus. Among hypertensive subjects, the E strain rate at basal, mid, and apex was significantly decreased. Systolic strain was significantly decreased at the septum in the hypertensive group. Conclusion: Strain rate might be a useful new parameter for the quantification of both regional and global LV functions and could be used in long-term follow up in hypertensive patients. Early identification by SRI of subjects at risk for hypertensive and ventricular dysfunction may help to stratify risk and guide therapy. Further studies, including serial assessment of LV structure and function in a larger number of adolescents with hypertension, is necessary.

Angiotensinogen gene M235T polymorphism as a predictor of cardiovascular risk in hypertensive adolescents (고혈압 청소년의 심혈관계 위험요소로서 Angiotensinogen M235T 유전자 다형)

  • Gil, Joo Hyun;Lee, Jung Ah;Park, Eun Young;Hong, Young Mi
    • Clinical and Experimental Pediatrics
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    • v.52 no.1
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    • pp.36-43
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    • 2009
  • urpose : The renin-angiotensin system (RAS) has been demonstrated to play a major role in regulating blood pressure. Therefore, components of the RAS are likely candidate genes that may predispose an individual to essential hypertension and cardiovascular complications. Among them, the M235T polymorphism of the angiotensinogen gene has been speculated to be associated with elevated circulating angiotensinogen concentrations and essential hypertension. This study aimed to analyze the angiotensinogen M235T polymorphism in hypertensive adolescents and investigate its relationship with cardiovascular risks. Methods : Forty Korean hypertensive adolescents (aged 16-17, systolic $BP{\geq}140 mmHg$ and/or diastolic $BP{\geq}90 mmHg$) and fifty seven normal adolescents were included. Obesity index (OI), body mass index (BMI) were calculated. BP was measured by oscillometric methods in resting state. Polymerase chain reaction (PCR) technique was performed on DNA from the hypertensives subjects to analyze the M235T polymorphism. Serum homocysteine, insulin, renin, aldosterone and angiotensin converting enzyme (ACE) were tested according to each genotype. The carotid intima-media thickness (IMT) and carotid artery diameter, Pulse wave velocity (PWV) and ankle-brachial index (ABI) were measured according to each genotype. Results : Genotype frequencies of T/T, M/T and M/M were 62.5%, 35.0%, 2.5%, respectively in hypertensive adolescents. The results were not significantly different compared to control group. Serum insulin, renin levels, BMI and OI were significantly higher in thoses with the M/M genotype as compared to those with the T/T of M/T genotype. Conclusion : This study showed that the M235T polymorphism was not associated with essential hypertension or any cardiovascular risks. Further clinical research is required to ascertain the relationship between this polymorphism and cardiovascular complications in Korean hypertensive adolescents.

Growth and Physiological Adaptations of Tomato Plants (Lycopersicon esculentum Mill) in Response to Water Scarcity in Soil (토양 수분 결핍에 따른 토마토의 생육과 생리적응)

  • Hwang, Seung-Mi;Kwon, Taek-Ryun;Doh, Eun-Soo;Park, Me-Hea
    • Journal of Bio-Environment Control
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    • v.19 no.4
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    • pp.266-274
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    • 2010
  • This study aim to investigate fundamentally the growth and physiological responses of tomato plants in responses to two different levels of water deficit, a weak drought stress (-25 kPa) and a severe drought stress (-100 kPa) in soil. The two levels of water deficit were maintained using a micro-irrigation system consisted of soil sensors for the real-time monitoring of soil water content and irrigation modules in a greenhouse experiment. Soil water contents were fluctuated throughout the 30 days treatment period but differed between the two treatments with the average -47 kPa in -25 kPa set treatment and the -119 kPa in -100 kPa set treatment. There were significant differences in plant height between the two different soil water statuses in plant height without differences of the number of nodes. The plants grown in the severe water-deficit treatment had greater accumulation of biomass than the plants in the weak water-deficit treatment. The severe water-deficit treatment (-119 kPa) also induced greater leaf area and leaf dry weight of the plants than the weak water-deficit treatment did, even though there was no difference in leaf area per unit dry weight. These results of growth parameters tested in this study indicate that the severe drought could cause an adaptation of tomato plants to the drought stress with the enhancement of biomass and leaf expansion without changes of leaf thickness. Greater relative water content of leaves and lower osmotic potential of sap expressed from turgid leaves were recorded in the severe water deficit treatment than in the weak water deficit treatment. This finding also postulated physiological adaptation to be better water status under drought stress. The drought imposition affected significantly on photosynthesis, water use efficiency and stomatal conductance of tomato plants. The severe water-deficit treatment increased PSII activities and water use efficiency, but decreased stomatal conductance than the weak water-deficit treatment. However, there were no differences between the two treatments in total photosynthetic capacity. Finally, there were no differences in the number and biomass of fruits. These results suggested that tomato plants have an ability to make adaptation to water deficit conditions through changes in leaf morphology, osmotic potentials, and water use efficiency as well as PSII activity. These adaptation responses should be considered in the screening of drought tolerance of tomato plants.

Effect of Shading Level on Growth and Morphological Characteristics of Ligularia fischeri Seedling (차광수준이 곰취 유묘의 생장 및 형태적 특성에 미치는 영향)

  • Song, Ki Seon;Jeon, Kwon Seok;Kim, Chang Hwan;Yoon, Jun Hyuck;Park, Yong Bae;Kim, Jong Jin
    • Journal of Bio-Environment Control
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    • v.23 no.2
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    • pp.88-94
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    • 2014
  • This study was carried out to survey the early growth characteristics of Ligularia fischeri, plants of half-shaded grounds, primarily used as functional wild edible greens and examine its shading treatment to transplant seedling. The shading treatment was regulated with the shading level(full sunlight, 35%, 50%, 75%, and 95% of full sunlight). According to the experiment, the height was the highest under 75% of shading (17.3 cm), and root diameter was the highest under full sunlight (2.13 mm). It was found that fresh weight (leaf, shoot, root and whole) was the highest (1.784 g, 1.330 g, 0.791 g, 3.905 g respectively ) under 50% of shading. In case of dry weight, leaf, shoot and whole dry weight were surveyed the highest under 35% of shading, and root dry weight was the highest under full sunlight. It was found that S/R ratio and moisture contents (leaf, shoot, root and whole) were the highest under 95% of shading. Leaf area was the highest under 50% of shading ($39.3cm^2$) and the higher shading level, the higher SLA and LAR. It was surveyed that the higher shading level, the thinner leaf thickness. In case of root characteristics, it was showed that the relatively high height growth under 35%~75% of shading, excepting 95% of shading. As a result of surveying the whole experiment, it is concluded that the early growth of L. fischeri is more effective in producing it under 50% of shading.