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Evaluation of Anti-wrinkle Effect of Peanut Shell Extract and Stability of the Extract in Cosmetic Products (땅콩 겉껍질 추출물의 주름개선 효능 및 화장품 제형에서 추출물의 안정성 평가)

  • Narae Han;Jin Young Lee;Mihyang Kim;Eun Young Choi;Bong-Jeun An;Yu-Young Lee;Moon Seok Kang;Hyun-Joo Kim
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.49 no.3
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    • pp.203-212
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    • 2023
  • This study was conducted to investigate the possibility of peanut shell, a by-product of peanut, as a functional cosmetic ingredient. Peanut shell extract showed high antioxidant activity with IC50 values of 75.00, 46.33, and 472.83 ㎍/mL for DPPH and ABTS radical scavenging and SOD-like activity, respectively. Furthermore, peanut shell extract was efficiently decreased the MMP-1 and MMP-3 protein level in the UVB treated-HaCaT cell and maintained procollagen protein level similar to normal control. Similar to anti-wrinkle related protein expression assay, the IC50 value of elastase and collagnease inhibition in peanut shell extract was lower as 0.30 and 0.09 mg/mL, respectively, than that of the positive control. Additionally, eriodictyol and luteolin, which are isolated from peanut shell extract, showed 53.8 and 98.0% elastase inhibition rate, respectively, and 60.1 and 72.5% collagenase inhibition rate, respectively, at a concentration of 0.1 mg/mL. Thus, luteolin was assumed to be the effective ingredient for wrinkle inhibition in peanut shell extract. As a result of stability evaluation of lotion and cream formulations containing peanut shell extract, it was confirmed to be a stable formulation with no significant changes. Therefore, it is considered that peanut shell extract can be applied as a cosmetic ingredient for wrinkle inhibition.

A stratified random sampling design for paddy fields: Optimized stratification and sample allocation for effective spatial modeling and mapping of the impact of climate changes on agricultural system in Korea (농지 공간격자 자료의 층화랜덤샘플링: 농업시스템 기후변화 영향 공간모델링을 위한 국내 농지 최적 층화 및 샘플 수 최적화 연구)

  • Minyoung Lee;Yongeun Kim;Jinsol Hong;Kijong Cho
    • Korean Journal of Environmental Biology
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    • v.39 no.4
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    • pp.526-535
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    • 2021
  • Spatial sampling design plays an important role in GIS-based modeling studies because it increases modeling efficiency while reducing the cost of sampling. In the field of agricultural systems, research demand for high-resolution spatial databased modeling to predict and evaluate climate change impacts is growing rapidly. Accordingly, the need and importance of spatial sampling design are increasing. The purpose of this study was to design spatial sampling of paddy fields (11,386 grids with 1 km spatial resolution) in Korea for use in agricultural spatial modeling. A stratified random sampling design was developed and applied in 2030s, 2050s, and 2080s under two RCP scenarios of 4.5 and 8.5. Twenty-five weather and four soil characteristics were used as stratification variables. Stratification and sample allocation were optimized to ensure minimum sample size under given precision constraints for 16 target variables such as crop yield, greenhouse gas emission, and pest distribution. Precision and accuracy of the sampling were evaluated through sampling simulations based on coefficient of variation (CV) and relative bias, respectively. As a result, the paddy field could be optimized in the range of 5 to 21 strata and 46 to 69 samples. Evaluation results showed that target variables were within precision constraints (CV<0.05 except for crop yield) with low bias values (below 3%). These results can contribute to reducing sampling cost and computation time while having high predictive power. It is expected to be widely used as a representative sample grid in various agriculture spatial modeling studies.

Residual evaluation of ethyl formate in soil and crops after fumigation in green house (에틸포메이트의 하우스 농작물 훈증처리 후 토양 및 작물 중 잔류양상)

  • Hwang-Ju Jeon;Kyeongnam Kim;Chaeeun Kim;Yerin Cho;Tae-Hyung Kwon;Byung-Ho Lee;Sung-Eun Lee
    • Korean Journal of Environmental Biology
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    • v.40 no.3
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    • pp.316-324
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    • 2022
  • Ethyl formate (EF) is a potent fumigant replacing methyl bromide. The use of EF is limited to a quarantine process. Appling EF to agricultural field as a safe insecticide in greenhouse give us valuable benefits including less residual concern. In this regard, residual pattern after EF fumigation in greenhouse should be undertaken. In the previous study, we have established agricultural control concentration of EF to control pests in a greenhouse. EF was fumigated at 5 g m-3 level for 2 h. The concentration of EF inside a greenhouse was analyzed to be 4.1-4.3 g m-3 at 30 min after fumigation. To prepare an analytical method for residues in cucumber crops and soil in the greenhouse, the limit of detection(LOD) of the method was 100ng g-1 and the limit of quantitation(LOQ) of this method was 300 ng g-1. R2 values of calibration curves for crops and soil were 0.991-0.997. In samples collected immediately after ventilation, EF concentration was determined to be below LOQ level. In addition, EF level was below LOQ in samples collected at 3 h after ventilation except that leaf samples of melon during the flowering period showed a level of 1,068.9 ng g-1. Taken together, these results indicate that EF used in quarantine can be applied to agricultural fields without residual issue as an effective fumigant for insect pest control.

Acute toxicity of ethyl formate to nontarget organisms and reduction effect of sodium silicate on ethyl formate-induced phytotoxicity (에틸포메이트의 비표적생물에 대한 급성독성 및 sodium silicate의 약해저감 효과)

  • Kyeongnam Kim;Yubin Lee;Yurim Kim;Donghyeon Kim;Chaeeun Kim;Yerin Cho;Junyeong Park;Yongha You;Byung-Ho Lee ;Sung-Eun Lee
    • Korean Journal of Environmental Biology
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    • v.41 no.3
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    • pp.193-203
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    • 2023
  • Ethyl formate (EF) is a naturally occurring insecticidal compound and is used to control pests introduced from abroad, in quarantine, by a fumigation method. In particular, it is mainly used as a substitute for methyl bromide and is less toxic to humans and less harmful to plants. This study aimed to investigate the possible acute toxicity of EF to useful organisms, and how to reduce phytotoxicity in watermelon, zucchini, and oriental melon. After fumigation with EF for 2 h, the LC50 values for earthworms, honey bees, and silkworms were 39.9, 7.09, and 17.9g m-3, respectively. The degree of susceptibility to EF was in the order of earthworms, silkworms, and honey bees based on the LC50 value, and EF fumigation induced stronger acute toxicity to honey bees. Phytotoxicity was observed in watermelon leaves treated with a concentration of 7.5 g m-3 EF, and when treated with a concentration of 10.0g m-3, it was confirmed that the edges of watermelon leaves were charred and seemed to be damaged by acids. Zucchini and melon, and other cucurbits, showed strong damage to the leaves when treated with a concentration of 10 g m-3, and sodium silicate, at concentrations of 10% and 20%, was used to reduce phytotoxicity. Therefore, acute toxicity towards nontarget organisms and phytotoxicity during the fumigation of EF should be reduced for efficient agricultural pest control.

Distribution of Heavy Metal Contents in Medicinal Plants and Soils with Soil Texture (약용작물(藥用作物)과 그 재배토양(栽培土壤)의 토성별(土性別) 중금속함량(重金屬含量) 분포(分布))

  • Jung, Goo-Bok;Kim, Bok-Young;Kim, Kyu-Sik;Lee, Jong-Sik;Ryu, In-Soo
    • Korean Journal of Soil Science and Fertilizer
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    • v.29 no.2
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    • pp.158-164
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    • 1996
  • This survey was conducted to investigate the distribution of heavy metal contents in medicinal plants and soils. Plant and soil samples were collected at 254 sites(Angelica gigas : 81, Astragalus membranceus : 38, Platycodn glandiflorum : 36, Paeonia albilora : 34, Codonopsis lanoceolata : 32, Ligusticum chuanxiong : 17, Bupleurum falcatum : 16, respectively) over the country, Soil texture, pH values and heavy metal content of soils and medicinal plants were evaluated as major factors. Soil texture classification showed that sandy loam, loam, loamy sand and silt loam were 46.1%, 26.0%, 19.3% and 8.6% of the total, respectively. The contents of O.M, Ex.Ca. Ex.Mg and EC value were higher in loamy(sandy loam, loam and silt loam)soils than in sandy(loamy sand)soils, but available $P_2O_5$ contents of loamy sand soils were higher than those of sandy loam, loam, and silt loam. The contents of Cd, Cu, Pb, Zn and Ni in soil were high in loamy soils, while Cr content was high in loamy silt soils. The contents of Cu and Cr in Angelica gigas were high in loamy soils, and those of Pb in Astragalus membranceus, Paeonia albiflora and Codonopsis lanoceolata were high in sandy loam soils. Correlation coefficients between heavy metal contents in medicinal plants and their soils with soil texture were positively correlated in sandy loam and loam at Cu, loam at Zn, sandy loam, loam and loamy sand at Cr, respectively. Correlation coefficients between pH value of the soils and contents of Cd, Zn and Ni in medicinal plants were negatively correlated, but those of Cd, Pb, Zn and Ni in soils were positively correlated.

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Comparisons of Soil Characteristics between Campsites and Their Adjoining Forest Areas in the Eastern Area of Jirisan National Park (지리산국립공원 동부지역 야영장 조성지와 인접 산림지역의 토양 특성 비교)

  • Kim, Choon-Sig;Jang, Kyoung-Su;Lee, Hong-Woo;Cho, Hyun-Seo
    • Korean Journal of Environment and Ecology
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    • v.21 no.6
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    • pp.487-493
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    • 2007
  • This study was carried out to evaluate soil property between campsites located at Joongsanri, Daewonsa old, Daewonsa new, Baekmudong and their adjoining forest areas in the eastern area of Jirisan National Park. The survey results showed that there existed a significant difference(p<0.05) between the two areas in soil property, such as soil bulk density, soil hardness, soil pH, water infiltration and soil respiration rates. However, there was no difference in soil property by depth between surface soil($0{\sim}15cm$) and subsurface soil($15{\sim}30cm$) for the two areas(p>0.05). In case of soil bulk density, its values in campsites were significantly higher ($1.29{\sim}1.44g/cm^3$) than in forest areas($0.95g/cm^3$), while soil hardness was significantly lower(p<0.05) in forest areas($1.44kg/cm^2$) than in campsites($2.9{\sim}4.0kg/cm^2$). Soil pore space was significantly lower in campsites($45.7{\sim}51.4%$) than in forest areas(64.3%), and soil pH in forest areas indicated pH 5.46 and that of the campsites was distributed at the range from pH 6.49 to pH 6.38. In addition, water infiltration was significantly lower in campsites($0.79{\sim}2.06cc/sec$) than in forest areas(18.7cc/sec), while soil respiration rates were significantly higher in forest areas($0.58gCO_2/m^2/h$) than in campsites($0.13{\sim}0.34gCO_2/m^2/h$).

On the Variation of Wood Fiber Dimensions of the Oak grown in Mt. Jiri (지리산산(智異山産) 참나무류(類)의 목섬유(木纖維) 변이(變移)에 관(關)하여)

  • Hong, Byung-Wha;Moon, Chang-Kuk;Shin, Dong-So
    • Journal of Korean Society of Forest Science
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    • v.14 no.1
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    • pp.33-38
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    • 1972
  • This study was carried out to investigate on the variation of wood fiber dimensions of some oakwoods. Every 2 annual ring was selected for specimens from pith toward bark up to 28th annual ring on 3 species of Oak SINGALNAMOO(Quercus mongorica Fisch.), JOLCHAMNAMOO(Quercus serrata Thunb.) and KULCHAMNAMOO(Quercus variabilis Blume) which grew in Mt. Jiri. The investgated results are as follows: 1. Values of wood fiber lengths increase rapidly up to 16th annual ring, however, considerably stable at the rear part. Range of wood fiber length variations: Species Range of length(mean) CV SINGALNAMOO, $640{\sim}1,544{\mu}$($1,142.3{\mu}$), 8~16% JOLCHAMNAMOO, $592{\sim}1,600{\mu}$($1,179.6{\mu}$), 6~21% KULCHAMNAMOO, $679{\sim}1,592{\mu}$($1,298.9{\mu}$), 2~15% 2. The increment of wood fiber width and thickness by annual rings shows quite inactive contrary to the length. Range of wood fiber width: $7.6{\sim}10.0{\mu}$(mean $9.3{\mu}$) Range of wood fiber thickness: $2.0{\sim}3.2{\mu}$(mean $2.6{\mu}$) 3. There was no significance between species through their wood fiber lengths. 4. And these wood fibers believed to be valuable for pulp-wood through their fiber bonding ratio, Runkel ratio and flexibility coefficient.

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Seasonal Variations of Epilithic Biofilm Biomass and Community Structure at Byeonsan Peninsula, Korea (한국 변산반도 암반생물막의 생물량과 군집구조의 계절 변화)

  • Kim, Bo Yeon;Park, Seo Kyoung;Lee, Jung Rok;Choi, Han Gil
    • Korean Journal of Environment and Ecology
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    • v.30 no.6
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    • pp.1009-1021
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    • 2016
  • The community structure and abundance of epilithic biofilm were bimonthly examined to know spatial and temporal patterns of biofilm biomass and taxonimical composition at the two study sites, Gosapo and Gyeokpo with different degrees of wave exposure levels from November 2010 to September 2011. Biomass was estimated by using chlorophyll a contents (Chl a), normalized difference vegetation index (NDVI), and vegetation index (VI). Cyanobacteria such as Aphanotece spp. predominated in the proportion of 57.53% at Gosapo and of 61.12% at Gyeokpo and they are abundant in mid shore and in summer at both study sites. The diatoms Navicula spp., Achnanthes spp. and Licmophora spp. were common species and they showed an increasing trend from high to low shore. NDVI, VI, and chl a contents were the greatest at mid shore for Gosapo (0.44, 3.05, $24.56{\mu}g/cm^2$) and at low shore for Gyeokpo (0.41, 2.73, $17.98{\mu}g/cm^2$). NDVI, VI, and chl a content were all maximal in January and minimal in March at the both sites. Average NDVI, VI, and chlorophyll a contents of biofilms were greater at Gosapo (0.43, 2.89, $22.84{\mu}g/cm^2$) than Gyeokpo (0.38, 2.48, $15.48{\mu}g/cm^2$).Of three shore levels(high, mid, and low) Chl a contents were positively correlated with NDVI and VI at the two study sites indicating that non-destructive NDVI and VI values can be used in stead of destructive Chl a extraction method. In conclusion, epilithic biofilm was more abundant seasonally in winter, vertically in mid and low intertidal zone, and horizontally at wave exposed shore than in summer, at high and sheltered shore in Korea.

A STUDY ABOUT ALVEOLAR CREST BONE HEIGHT BEFORE AND AFTER ORTHODONTIC TREATMENT BY USING BITEWING FILM (교익사진을 이용한 교정치료 전후의 치조골 높이 변화에 관한 연구)

  • Hwang, Chung-Ju
    • The korean journal of orthodontics
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    • v.27 no.3 s.62
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    • pp.421-430
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    • 1997
  • Alveolar bone grows with development of tooth germs and roots; bone deposition occurs with tooth eruption. Bone components undergoes processes of resorption and deposition, and when the balance between them is disrupted, decrease in alveolar bone height or excessive bone deposition result. It has been hon that repositioning of teeth through orthodontic treatment can cause alveolar bone resorption which result in decreased alveolar bone height, and there have been many studies to evaluate such effects. X-ray films that could be replicated and standardized were chosen in clinical studies, and among them, bitewing films were used for objective evaluation of changes in alveolar bone level. Twenty subjects, 10 to 13-year- old (average 12.2) children with Cl I molar key, healthy oral condition, no congenital missing, no periodontal disease, and pre-and post-orthodontic bitewing films, were randomly selected for comparison of alveolar bone heights. Amounts of tooth and changes in alveolar bone heights were analyzed. The following results were obtained: 1. Amount of tooth movement in canine, premolar, and molar regions, changes in tooth axis, and changes in alveolar bone heights were measured, and the mean and median values were obtained. 2. When pre-and post-orthodontic alveolar bone levels were compared, larger changes were noticed in maxilla than mandible. 3. When mesio-distally compared, larger changes were observed in the distal sides of 3D3 and 4M3, mesial sides of 4M3 and 4D3, distal sides of 4D3 and 5M3, mesial sides of 5M3 and 5D3, md distal sides of 5D3 and 6M3. 4. When the amounts of tooth movements(TX, TY)and changes in tooth axis(A) were compared,34TX, 34TY, 34A of both sides in maxilla were greater, iud changes in alveolar bone level were greater than any other region.

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Changes of Physicochemical Properties during Fermentation of Peach Wine and Quality Improvement by Ultrafiltration (복숭아주 발효시 이화학적 특성변화와 한외여과에 의한 품질 향상)

  • 정재호;목철균;임상빈;박영서
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.4
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    • pp.506-512
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    • 2003
  • Peach wine was fermented at $25^{\circ}C$ for 2 weeks using Saccharomyces cerevisiae KCCM 12224, aged at 15$^{\circ}C$ for 14 weeks, and its physicochemical and microbiological changes were investigated. The viable bacterial cell numbers, 1.4$\times$10$^3$ CFU/mL at the beginning of fermentation, increased to 2.8$\times$10$^{6}$ CFU/mL after 2 weeks, but decreased to 7.0$\times$10$^3$ CFU/mL after 14 weeks. The viable yeast cell numbers were changed from 3.4$\times$10$^2$ CFU/mL to 2.4$\times$10$^{7}$ CFU/mL during fermentation, and decreased to 4.0$\times$10$^4$ CFU/mL after aging. Turbidity total sugar content, reducing sugar content, solid content and b value of peach wine decreased during fermentation but acidity, alcohol content, L and a value increased. Most physicochemical properties except alcohol content and reducing sugar content were not changed significantly during aging. When peach wine was filtered through 0.45 ${\mu}{\textrm}{m}$ nitrocellulose membrane followed by various ultrafiltration membranes with different molecular weight cut-off values, Biomax 100K membrane, with 79 liter/$m^2$/h (LMH) of initial flux, was suitable for ultrafiltration process of peach wine. These membrane filtration treatments resulted in complete removal of microorganisms and decrease in turbidity and alcohol content without changes in other chemical properties. The physicochemical properties of peach wine were not changed and any microorganisms were not found during the storage at 3$0^{\circ}C$ for 12 Weeks.