• 제목/요약/키워드: The Structural Lineage

검색결과 10건 처리시간 0.027초

Diversification and domain evolution of molluskan metallothioneins: a mini review

  • Nam, Yoon Kwon;Kim, Eun Jeong
    • Fisheries and Aquatic Sciences
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    • 제20권6호
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    • pp.8.1-8.18
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    • 2017
  • Background: Metallothionein (MT) is a multifunctional protein playing important roles in homeostatic regulation and detoxification of metals. Mollusk species have been considered as useful sentinel platforms for MT-based biomarker approaches, and they have been reported to display an extraordinary structural diversity of MT proteins. However, potential diversity of molluskan MTs has not been fully explored and recent updates have suggested the need of revision of evolutionary hypothesis for molluskan MTs. Results: Based on bioinformatic analysis and phylogenetic evidences, novel divergence mechanisms and paths were hypothesized in both gastropod and bivalve MT groups. Our analyses are suggestive of the taxon- or lineage-specific domain multiplication/duplication from the ancestral or prototypic MT. Diversification and selection of molluskan MTs might be driven by the needs for acquiring metal selectiveness, specialized novel function, and improved capacity of metal detoxification under environmentally stressed conditions. Conclusion: The structural diversity and variations of molluskan MTs are significantly larger than previously understood. Undoubtedly, molluskan MTs have undergone dynamic divergent processes in their evolutionary histories, giving rise to the great diversity of domain structures in extant MT isoforms. Novel evolutionary paths for molluskan MTs newly proposed in this review could shed additional light onto the revision of the hypothesis for evolutionary differentiation of MTs in the molluskan lineage.

Cytogenetic Profile of De Novo B lineage Acute Lymphoblastic Leukemia: Determination of Frequency, Distribution Pattern and Identification of Rare and Novel Chromosomal Aberrations in Indian Patients

  • Bhandari, Prerana;Ahmad, Firoz;Dalvi, Rupa;Koppaka, Neeraja;Kokate, Prajakta;Das, Bibhu Ranjan;Mandava, Swarna
    • Asian Pacific Journal of Cancer Prevention
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    • 제16권16호
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    • pp.7219-7229
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    • 2015
  • Background: Chromosomal aberrations identified in acute lymphoblastic leukemia (ALL) have an important role in disease diagnosis, prognosis and management. Information on karyotype and associated clinical parameters are essential to physicians for planning cancer control interventions in different geographical regions. Materials and Methods: In this study, we present the overall frequency and distribution patterns of chromosomal aberrations in both children and adult de novo B lineage ALL Indian patients using conventional cytogenetics, interphase FISH and multiplex RT-PCR. Results: Among the 215 subjects, cytogenetic results were achieved in 172 (80%) patients; normal karyotype represented 37.2% and abnormal 62.8% with a distribution as follows: 15.3% hypodiploidy; 10.3% hyperdiploidy; 15.8% t(9;22); 9.8% t(1;19); 3.7% t(12;21); 2.8% t(4;11); 2.8% complex karyotypes. Apart from these, we observed several novel, rare and common chromosomal rearrangements. Also, FISH studies using LSI extra-signal dual-color probes revealed additional structural or numerical changes. Conclusions: These results demonstrate cytogenetic heterogeneity of ALL and confirm that the incidence of chromosomal abnormalities varies considerably. To the best of our knowledge, this is one of the largest reported series of cytogenetic investigations in Indian B-lineage ALL cases. In addition, ongoing cytogenetic studies are warranted in larger groups of B-lineage ALL cases to identify newly acquired chromosomal abnormalities that may contribute to disease diagnosis and management.

본부와 방면 - 대순진리회 종교조직의 특성 - (Bonbu and Bangmyeon: The Lineage Principle in Daesoon Jinrihoe)

  • 에드워드 아이런
    • 대순사상논총
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    • 제35집
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    • pp.427-476
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    • 2020
  • 대순진리회는 1969년 박우당에 의해 공식적으로 창설되었다. 이 글은 대순진리회가 증산을 신앙하는 다른 종단과 역사와 사상을 공유하고 있지만 조직 측면에서 타종단과 구별됨을 보여주고자 한다. 대순진리회 조직의 양대 기구인 본부와 방면은 대순진리회가 신종교로서 급속한 성장을 이루는 데 서로 기여했다. 본부는 중앙 집권적인 위계질서가 있는 반면, 방면은 친족 같은 강한 충성도와 공동체의식을 보여준다. 두 조직 기구에 공통적인 특성은 계보(lineage) 개념으로 이는 대순진리회 양대 조직의 분절화 원리이다. 미세하게 균형 잡힌 두 조직기구는 대순진리회의 종교적 사명 실현에 크게 기여했다. 균형의 첫 번째 축은 본부이다. 본부는 여주와 여주 외 지역 도장 및 수련센터의 핵심 종단 기구를 포함한다. 이러한 기구들은 모두 박우당 도전에 의해 설립되었다. 박우당 도전은 종단 운영의 제반사항을 총괄하는 종단의 헌법 곧 도헌을 제정하였다. 중앙종의회로부터 포덕, 감사, 기획 등에 이르는 본부의 제반 운영은 박우당 도전의 운영지침에 충실히 부합한다. 균형의 두 번째 축은 방면이다. 비교적 대규모 방면들은 그 자체가 복합적인 조직이다. 이 연구의 결론은 본부와 방면이라는 양대 축에 대한 조직유형적 특성구분이다. 로버트 퀸과 킴 카메론의 분류에 따른다면 대순진리회의 본부는 효율성을 중시하는 전형적인 중앙집권화 조직이다. 이에 반해 방면은 집단정체성과 공동체의식을 중시하는 친족에 가까운 조직이다.

Structure and Function of the Influenza A Virus Non-Structural Protein 1

  • Han, Chang Woo;Jeong, Mi Suk;Jang, Se Bok
    • Journal of Microbiology and Biotechnology
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    • 제29권8호
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    • pp.1184-1192
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    • 2019
  • The influenza A virus is a highly infectious respiratory pathogen that sickens many people with respiratory disease annually. To prevent outbreaks of this viral infection, an understanding of the characteristics of virus-host interaction and development of an anti-viral agent is urgently needed. The influenza A virus can infect mammalian species including humans, pigs, horses and seals. Furthermore, this virus can switch hosts and form a novel lineage. This so-called zoonotic infection provides an opportunity for virus adaptation to the new host and leads to pandemics. Most influenza A viruses express proteins that antagonize the antiviral defense of the host cell. The non-structural protein 1 (NS1) of the influenza A virus is the most important viral regulatory factor controlling cellular processes to modulate host cell gene expression and double-stranded RNA (dsRNA)-mediated antiviral response. This review focuses on the influenza A virus NS1 protein and outlines current issues including the life cycle of the influenza A virus, structural characterization of the influenza A virus NS1, interaction between NS1 and host immune response factor, and design of inhibitors resistant to the influenza A virus.

고려(高麗) 금동탑(金銅塔)을 통해 본 법주사(法主寺) 팔상전(捌相殿)의 구조형식계통(構造形式系統) (The Structural Lineage of Palsangjeon in Pubjoo Temple Analyzed through Gilt-bronze Pagoda in the Koryo Period)

  • 김경표
    • 건축역사연구
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    • 제14권1호
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    • pp.89-105
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    • 2005
  • The central aim of this thesis is to see if the structure of Palsangjeon(捌相殿) in Pubjoo Temple(法住寺), a five sto wooden pagoda in Chosen(朝鮮) Dynasty, was handed down from the ancient and middle ages. This study was performed through an analysis of Gilt-Bronze Pagoda built in Koryo(高麗) period. In other words, it is aimed at analyzing which lineage the structure of Palsangjeonbelongs to as a wooden pagoda. In analyzing the structure of Palsangjeon, I attempted to find out its source from the remains of Koryo period prior to the Chosen Dynasty. Examples are the Gilt-Bronze Pagoda, built during the Koryo period. I have also examined its relationship with other existing wooden pagodas and remains. The analysis of Palsangjeon, a five story wooden pagoda in Chosen Dynasty, focuses on the following: First, I explored the possibilities of whether the structure of Palsangjeon was newly invented in Chosen Dynasty, or if it had been derived from the wooden pagodas in the Koryo period. Secondly, I tried to find out if the stable vertical planes, with a great successive diminution ratio, were derived from the middle age, i.e. Koryo period. The results of the study of Palsangjeon through Gilt-Bronze Pagoda analysis are as follows: 1. The structure of Gilt-Bronze Pagoda, a wooden pagoda from the Koryo period, is roughly classified into the accumulation type, using pipe pillars, and the one story type using whole pillars. In the accumulation type, stories are connected in either a flat format or an intervening format. The Gilt-Bronze Pagoda is mainly composed of pipe pillars, with some whole pillars. However, the central pillar was omitted in the building structure. Generally, the upper and lower stories are connected by pipe pillars in a crutch format. All the pillars, whether they are pipe pillars or whole pillars, used Naiten(內轉) technology. The Eave supporter has the Haang type(下昻) and the Muhaang type(無下昻). In most cases, high balustrades are furnished, but few tables of high balustrades have been found. The slanting roof formats have been handed down from Paekche(百濟), Silla(新羅), or Koryo(高麗). However, the structure of the octagon is assumed to be derived from Koguryo(高句麗). The structure of the Gilt-Bronze Pagoda from the Koryo period is mainly composed of accumulated flat squares, with some spire types. intervening format, the structure of Palsangjeon used whole pillars in a half story format in which upper level side pillars are installed on the lower level tie beam. From the Bronze Pagoda from the Koryo period, we can assume that the half story format of wooden pagodas that has stable vertical planes with a great successive diminution ratio was created during the mid-Koryo period at the latest and had been idly developed by the time of the Chosen Dynasty. 3. The whole pillars in Palsangjeon are also found in Gilt-Bronze Pagodas from the Koryo period. Hence, all of the pillars in Palsangjeon seem to have been handed down from the ancient construction technology. They were also used in the construction of wooden pagodas from the Koryo period. Therefore, it is assumed that Palsangjeon was constructed using the construction technology of the Chosen Dynasty that had been developed from the wooden pagoda construction technology of the Koryo period. The stable vertical planes with a great successive diminution ratio in Palsangjeon are derived from ancient Korean wooden pagodas, which have developed into indigenous Korean wooden pagodas with fairly stable vertical planes and a great design, in the half story format of Koryo and Chosen Dynasty. Therefore, it is assumed that the structure of Palsangjeon has a systematic relationship with traditional Korean wooden pagodas and is one of the indigenous Korean wooden pagoda structures. 4. In China, the intervening format has been mainly used between stories in multi-story architecture since the ancient days. At the same time, the flat format as also used in ancient and middle ages. However, the flat format was replaced by whole pillars during the Ming(明) and Manchu(淸) Dynasties, in favor of simple and compact construction. The half-story format, in which upper level side pillars are installed on tie beams, has been found in some cases, but it doesn't seem to have been the primary construction technology. Few traces of the half-story format have been found in multi-story architecture in Japan, and it has not been used as a general construction format. By contrast, the half-story format, which seems to have been derived from the Koryo period, was used as a general construction format in multi-story architecture of the Chosen Dynasty. The construction technology of multi-story architecture is related to that of multi-story wooden pagodas, but they have different production technologies. It seems that the structure of Palsangjeon did not just adopt the construction technology of multi-story architecture in the Chosen Dynasty, but it was developed from wooden pagodas in the Koryo period, including the Gilt-Bronze Pagoda. 5. Since the ancient days, most Chinese and Japanese wooden pagodas have adopted an accumulation type of structure using pipe pillars, with accumulated pointed towers. On the other hand, though most Korean wooden pagodas have also adopted an accumulation type of structure from the ancientdays, one story type using whole pillars was created in the Koryo and Chosen Dynasties. The wooden pagoda structure of Palsangjeon, with stable vertical planes in a half story format, is a unique Korean construction technology, different from the construction technologies of Chinese and Japanese wooden pagodas. This thesis clearly determined the structural characteristics of Palsangjeon. However, various remains have yet to be analyzed in depth, to establish an accurate construction technology system. In the beginning of this thesis, I had difficulty in precisely interpreting the internal structure of the Gilt-Bronze Pagoda from its appearance. However, in the process of study, the more serious problem was that there are few remains or ruins of multi-story architecture in ancient and the middle ages of Korea. Therefore, it is urgent to discover various remains in the future. This thesis succeeded in determining the structural characteristics of Palsangjeon. However, it fell short of clarifying the structural lineage of the stable vertical planes, although they show indigenous Korean architectural taste, representing the unique national emotion, and the construction format of multi-story wooden pagodas in Korea. I hope this is clarified in the future research.

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Color Sensing and Signal Transmission Diversity of Cyanobacterial Phytochromes and Cyanobacteriochromes

  • Villafani, Yvette;Yang, Hee Wook;Park, Youn-Il
    • Molecules and Cells
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    • 제43권6호
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    • pp.509-516
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    • 2020
  • To perceive fluctuations in light quality, quantity, and timing, higher plants have evolved diverse photoreceptors including UVR8 (a UV-B photoreceptor), cryptochromes, phototropins, and phytochromes (Phys). In contrast to plants, prokaryotic oxygen-evolving photosynthetic organisms, cyanobacteria, rely mostly on bilin-based photoreceptors, namely, cyanobacterial phytochromes (Cphs) and cyanobacteriochromes (CBCRs), which exhibit structural and functional differences compared with plant Phys. CBCRs comprise varying numbers of light sensing domains with diverse color-tuning mechanisms and signal transmission pathways, allowing cyanobacteria to respond to UV-A, visible, and far-red lights. Recent genomic surveys of filamentous cyanobacteria revealed novel CBCRs with broader chromophore-binding specificity and photocycle protochromicity. Furthermore, a novel Cph lineage has been identified that absorbs blue-violet/yellow-orange light. In this minireview, we briefly discuss the diversity in color sensing and signal transmission mechanisms of Cphs and CBCRs, along with their potential utility in the field of optogenetics.

Production and characterization of monoclonal antibodies against an avian influenza virus (H9N2)

  • Lim, Yong Hwan;Phan, Le Van;Mo, In-Pil;Koo, Bon-Sang;Choi, Young-Ki;Lee, Seung-Chul;Kang, Shien-Young
    • 한국동물위생학회지
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    • 제40권3호
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    • pp.187-192
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    • 2017
  • In this report, fifteen monoclonal antibodies (MAbs) against an avian influenza virus (H9N2 subtype) were newly produced and characterized. These MAbs proved to react to the epitopes of nucleocapsid protein (NP), hemagglutinin (HA), neuraminidase (NA) and non-structural protein 1 (NS1) of Korean H9N2 strain, respectively. Two HA-specific MAbs showed the ability to inhibit the hemagglutination activity of H9N2 subtype avian influenza virus when tested by hemagglutination inhibition (HI) assay. All MAbs did not cross-react with other avian-origin viruses (Newcastle disease virus, infectious bursal disease virus, infectious bronchitis virus and avian rotavirus) by immunofluorescence test or enzyme-linked immunosorbent assay. The MAbs produced in this study could be useful as the materials for diagnostics and therapeutics against Korean-lineage H9N2 virus infections.

Glycogen Synthase Kinase-3 Interaction Domain Enhances Phosphorylation of SARS-CoV-2 Nucleocapsid Protein

  • Jun Seop, Yun;Hyeeun, Song;Nam Hee, Kim;So Young, Cha;Kyu Ho, Hwang;Jae Eun, Lee;Cheol-Hee, Jeong;Sang Hyun, Song;Seonghun, Kim;Eunae Sandra, Cho;Hyun Sil, Kim;Jong In, Yook
    • Molecules and Cells
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    • 제45권12호
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    • pp.911-922
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    • 2022
  • A structural protein of SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2), nucleocapsid (N) protein is phosphorylated by glycogen synthase kinase (GSK)-3 on the serine/arginine (SR) rich motif located in disordered regions. Although phosphorylation by GSK-3β constitutes a critical event for viral replication, the molecular mechanism underlying N phosphorylation is not well understood. In this study, we found the putative alpha-helix L/FxxxL/AxxRL motif known as the GSK-3 interacting domain (GID), found in many endogenous GSK-3β binding proteins, such as Axins, FRATs, WWOX, and GSKIP. Indeed, N interacts with GSK-3β similarly to Axin, and Leu to Glu substitution of the GID abolished the interaction, with loss of N phosphorylation. The N phosphorylation is also required for its structural loading in a virus-like particle (VLP). Compared to other coronaviruses, N of Sarbecovirus lineage including bat RaTG13 harbors a CDK1-primed phosphorylation site and Gly-rich linker for enhanced phosphorylation by GSK-3β. Furthermore, we found that the S202R mutant found in Delta and R203K/G204R mutant found in the Omicron variant allow increased abundance and hyper-phosphorylation of N. Our observations suggest that GID and mutations for increased phosphorylation in N may have contributed to the evolution of variants.

황룡사 창건 구층목탑 단상 (A Study on the Architecture of the Original Nine-Story Wooden Pagoda at Hwangnyongsa Temple)

  • 이주헌
    • 헤리티지:역사와 과학
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    • 제52권2호
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    • pp.196-219
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    • 2019
  • "삼국유사(三國遺事)"에 의하면 황룡사 구층목탑은 백제의 장인 아비지(阿非知)에 의해 선덕여왕 13년(645)에 건립된 것으로 전한다. 이후 고려 고종 25년(1238) 몽고군의 침략으로 사찰이 완전히 불타버리기 전까지 약 700년 동안 당시대인의 정신문화 속에서 최고의 자리를 차지하는 상징이자 호국불교의 구심체로서 존재하였다. 그동안 많은 연구자들이 문헌기록과 발굴조사 자료를 근거로 황룡사 창건 목탑에 대한 구조와 전체적인 형태에 대한 다양한 복원안을 제시해왔다. 하지만 그들이 제시한 황룡사 목탑의 모습은 기본적으로 현재 남아 있는 목탑지의 외형적인 형태와 단순한 목구조를 가정한 사각구층의 형태였다. 이는 관련 자료의 부족과 제한된 지식으로 인해 황룡사 창건 목탑의 기술적 계보에 대한 충분한 인식이 부족한 시기에 이루어진 것이어서 새로운 자료의 발견과 고대 대형 불탑의 구조 체계에 대한 인식 정도에 따라 충분히 수정될 수 있을 것으로 생각한다. 본고에서는 2005년부터 진행된 <황룡사 복원 연구>를 통해 정리된 목탑지에 대한 재분석 자료를 근거로 동아시아 고대 불탑의 구조 체계에 대한 폭넓은 이해 속에서 황룡사 창건 목탑의 계보와 구조에 대한 새로운 인식이 필요함을 제안해보고자 한다. 황룡사 창건 목탑은 통일신라와 고려시대에 걸쳐 적어도 두 번의 큰 중창을 겪는 동안 기단의 규모와 평면 형식 등이 변화된 것으로 추정되며, 이에 따라 상부 구조에도 큰 변화가 있었을 것으로 파악된다. 발굴조사 결과 드러난 유구에서 삼국시대에 만들어진 것으로 전하는 구층목탑과 관련된 흔적은 현재 판축으로 조성된 기단토와 심초석 정도이며, 그외 초석과 기단 석재 등 대부분은 9세기 이후의 것일 가능성이 높다. 따라서 황룡사 창건 목탑의 실질적인 구조는 당시 동아시아 대형 불탑의 변화과정 속에서 이해하여야 하며, 기술적 계보는 북조의 영향을 받은 백제의 대형 불탑에서 찾아야 할 것이다. 나아가 남산 탑곡 마애조상군에 표현된 9층 마애탑의 모습과 황룡사지 강당지에서 출토된 금동제 불탑 난간편 분석을 통하여 파악된 고고자료를 근거로 황룡사 창건 목탑의 이미지를 추정해본다면 외형상으로뿐만 아니라 구조적으로도 지금까지 우리가 인식하고 있던 순수한 목구조를 한 사각구층탑과는 다른 형태로 파악되는데, 아마도 실심체를 갖춘 이원 구조의 팔각구층탑일 것으로 파악된다.

가족 내 세대갈등과 통합 (Intergenerational Conflict and Integration in family)

  • 남순현
    • 한국심리학회지 : 문화 및 사회문제
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    • 제10권2호
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    • pp.1-15
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    • 2004
  • 최근 급변하는 사회현상들로 인한 다양한 가족유형들은 가족에 대한 개념 자체를 변화시키고 있다. 이러한 변화는 필연적으로 가족내 세대간 가치차이를 야기하며, 가족갈등이나 사회문제로 파생되는 것이 사실이다. 본고에서는 다양화, 다각화로 변모하고 있는 사회현상으로 인한 가족내 변화에 따른 가족내 세대간의 문제를 다루어봄으로써 세대갈등에 대한 해결책을 모색해보고자 한다. 먼저, 현대사회의 가족구조적인 변화가 가족내 어떤 기능적인 변화를 요구하고 있는지 살펴본 결과, 다음과 같은 긍정적인 변화들을 촉구하고 있었다. 첫째, 가족구성원들간의 상호작용하는 방식의 변화이고, 둘째, 가족의 심리적 자원으로서 애정, 보살핌과 같은 관계적 자원에 대한 지속적인 연속성의 요구이며, 셋째, 형식적 관계에서 벗어나 실질적 관계로의 전환, 및 마지막으로, 보다 명료한 의사소통망의 활용이었다. 또한 가족생애주기의 변화에 따른 부모-자녀관계의 상호작용을 재조명해봄으로써 세대갈등에 대한 해결책을 탐색해본 결과, 첫째, 현대사회의 다양한 가족유형의 가변성을 수용하고, 변화하는 가족의 기능적인 측면을 강조하여야 하며, 둘째, 각 세대가 지니고 있는 상호교환적인 가치를 수용하여 세대간 관계적 기능을 강화할 뿐만 아니라, 셋째, 효에 대한 재조명이 필요했다. 즉 가족기능의 세대간 전수는 가계와 가문형성의 근원이 될 수 있지만, 세대간의 상호작용없이 불가능하다.

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