Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
권호 표지
DOI QR코드

DOI QR Code

Production and characterization of monoclonal antibodies against an avian influenza virus (H9N2)

  • Lim, Yong Hwan (College of Veterinary Medicine, Chungbuk National University) ;
  • Phan, Le Van (College of Veterinary Medicine, Chungbuk National University) ;
  • Mo, In-Pil (College of Veterinary Medicine, Chungbuk National University) ;
  • Koo, Bon-Sang (National Primate Research Center, Korea Research Institute of Bioscience & Biotechnology) ;
  • Choi, Young-Ki (College of Medicine, Chungbuk National University) ;
  • Lee, Seung-Chul (Choong-Ang Vaccine Laboratories Inc.) ;
  • Kang, Shien-Young (College of Veterinary Medicine, Chungbuk National University)
  • Received : 2017.07.13
  • Accepted : 2017.09.11
  • Published : 2017.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

In this report, fifteen monoclonal antibodies (MAbs) against an avian influenza virus (H9N2 subtype) were newly produced and characterized. These MAbs proved to react to the epitopes of nucleocapsid protein (NP), hemagglutinin (HA), neuraminidase (NA) and non-structural protein 1 (NS1) of Korean H9N2 strain, respectively. Two HA-specific MAbs showed the ability to inhibit the hemagglutination activity of H9N2 subtype avian influenza virus when tested by hemagglutination inhibition (HI) assay. All MAbs did not cross-react with other avian-origin viruses (Newcastle disease virus, infectious bursal disease virus, infectious bronchitis virus and avian rotavirus) by immunofluorescence test or enzyme-linked immunosorbent assay. The MAbs produced in this study could be useful as the materials for diagnostics and therapeutics against Korean-lineage H9N2 virus infections.

Keywords

References

  1. Aymard-Henry M, Coleman M, Dowdle W, Laver W, Schild G, Webster R. 1973. Influenza virus neuraminidase and neuraminidase-inhibition test procedures. Bull World Health Organ 48: 199.
  2. Bright RA, Medina MJ, Xu X, Perez-Oronoz G, Wallis TR, Davis XM, Povinelli L, Cox NJ, Klimov AI. 2005. Incidence of adamantane resistance among influenza A (H3N2) viruses isolated worldwide from 1994 to 2005: a cause for concern. Lancet 366: 1175-1181. https://doi.org/10.1016/S0140-6736(05)67338-2
  3. Butt K, Smith GJ, Chen H, Zhang L, Leung YC, Xu K, Lim W, Webster RG, Yuen K, Peiris JM. 2005. Human infection with an avian H9N2 influenza A virus in Hong Kong in 2003. J Clin Microbiol 43: 5760-5767. https://doi.org/10.1128/JCM.43.11.5760-5767.2005
  4. Carrat F, Flahault A. 2007. Influenza vaccine: the challenge of antigenic drift. Vaccine 25: 6852-6862. https://doi.org/10.1016/j.vaccine.2007.07.027
  5. Chen R, Holmes EC. 2006. Avian influenza virus exhibits rapid evolutionary dynamics. Mol Biol Evol 23: 2336-2341. https://doi.org/10.1093/molbev/msl102
  6. Choi JG, Lee YJ, Kim YJ, Lee EK, Jeong OM, Sung HW, Kim JH, Kwon JH. 2008. An inactivated vaccine to control the current H9N2 low pathogenic avian influenza in Korea. J Vet Sci 9: 67-74. https://doi.org/10.4142/jvs.2008.9.1.67
  7. Gavin PJ, Thomson RB. 2004. Review of rapid diagnostic tests for influenza. Clin Appl Immunol Rev 4: 151-172. https://doi.org/10.1016/S1529-1049(03)00064-3
  8. Glikmann G, Chen S, Mordhorst CH, Koch C. 1995. Monoclonal antibodies for the rapid diagnosis of influenza-B virus infections by ELISA: production and characterization. Clin Diagn Virol 4: 27-42. https://doi.org/10.1016/0928-0197(94)00053-W
  9. Guan Y, Shortridge KF, Krauss S, Webster RG. 1999. Molecular characterization of H9N2 influenza viruses: were they the donors of the "internal" genes of H5N1 viruses in Hong Kong? Proc Natl Acad Sci 96: 9363-9367. https://doi.org/10.1073/pnas.96.16.9363
  10. Hiernaux J, Bona CA. 1982. Shared idiotypes among monoclonal antibodies specific for different immunodominant sugars of lipopolysaccharide of different Gram-negative bacteria. Proc Natl Acad Sci 79: 1616-1620. https://doi.org/10.1073/pnas.79.5.1616
  11. Huang Y, Li X, Zhang H, Chen B, Jiang Y, Yang L, Zhu W, Hu S, Zhou S, Tang Y. 2015. Human infection with an avian influenza A (H9N2) virus in the middle region of China. J Med Virol 87: 1641-1648. https://doi.org/10.1002/jmv.24231
  12. Ison MG, Gubareva LV, Atmar RL, Treanor J, Hayden FG. 2006. Recovery of drug-resistant influenza virus from immunocompromised patients: a case series. J Infect Dis 193: 760-764. https://doi.org/10.1086/500465
  13. Kohler G, Milstein C. 1975. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256: 495-497. https://doi.org/10.1038/256495a0
  14. Le VP. 2006. Expression of avian influenza virus (H9N2) genes and its diagnostic application. Chungbuk National University, Thesis of Master of Science.
  15. Lee CW, Song CS, Lee YJ, Mo IP, Garcia M, Suarez DL, Kim SJ. 2000. Sequence analysis of the hemagglutinin gene of H9N2 Korean avian influenza viruses and assessment of the pathogenic potential of isolate MS96. Avian Dis 44: 527-535. https://doi.org/10.2307/1593091
  16. Lee DH, Fusaro A, Son CS, Suarez DL, Swayne DE. 2016. Poultry vaccination directed evolution of H9N2 low pathogenicity avian influenza viruses in Korea. Virology 488: 225-231. https://doi.org/10.1016/j.virol.2015.11.023
  17. Lee DH, Song CS. 2013. H9N2 avian influenza virus in Korea: evolution and vaccination. Clin Exp Vaccine Res 2: 26-33. https://doi.org/10.7774/cevr.2013.2.1.26
  18. Lee YJ, Shin JY, Song MS, Lee YM, Choi JG, Lee EK, Jeong OM, Sung HW, Kim JH, Kwon YK. 2007. Continuing evolution of H9 influenza viruses in Korean poultry. Virology 359: 313-323. https://doi.org/10.1016/j.virol.2006.09.025
  19. Nguyen LT, Nakaishi K, Motojima K, Ohkawara A, Minato E, Maruyama J, Hiono T, Matsuno K, Okamatsu M, Kimura T, Takada A, Sakoda Y. 2017. Rapid and broad detection of H5 hemagglutinin by an immunochromatographic kit using novel monoclonal antibody against highly pathogenic avian influenza virus belonging to the genetic clade 2.3.4.4.. PLoS One 12: e0182228. https://doi.org/10.1371/journal.pone.0182228
  20. Obenauer JC, Denson J, Mehta PK, Su X, Mukatira S, Finkelstein DB, Xu X, Wang J, Ma J, Fan Y. 2006. Large-scale sequence analysis of avian influenza isolates. Science 311: 1576-1580. https://doi.org/10.1126/science.1121586
  21. Qiu X, Wong G, Audet J, Bello A, Fernando L, Alimonti JB, Fausther-Bovendo H, Wei H, Aviles J, Hiatt E. 2014. Reversion of advanced Ebola virus disease in nonhuman primates with ZMapp. Nature 514: 47-53. https://doi.org/10.1038/nature13777
  22. Truelove S, Zhu H, Lessler J, Riley S, Read JM, Wang S, Kwok KO, Guan Y, Jiang CQ, Cummings DAT. 2016. A comparision of hemagglutination inhibition and neutralization assays for characterizing immunity to seasonal influenza A. Influenza Other Respir Viruses 10: 518-524. https://doi.org/10.1111/irv.12408
  23. Xu K, Smith G, Bahl J, Duan L, Tai H, Vijaykrishna D, Wang J, Zhang J, Li K, Fan X. 2007. The genesis and evolution of H9N2 influenza viruses in poultry from southern China, 2000 to 2005. J Virol 81: 10389-10401. https://doi.org/10.1128/JVI.00979-07