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Functional Screening of Plant Genes Suppressed Salt Sensitive Phenotype of Calcineurin Deficient Mutant through Yeast Complementation Analysis (애기장대의 염해 저항성 관련 유전자의 기능적 선별)

  • Moon, Seok-Jun;Park, Soo-Kwon;Hwang, Un-Ha;Lee, Jong-Hee;Han, Sang-Ik;Nam, Min-Hee;Park, Dong-Soo;Shin, Dongjin
    • Journal of Life Science
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    • v.23 no.1
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    • pp.1-7
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    • 2013
  • Understanding salt tolerance mechanisms is important for the increase of crop yields, and so, several screening approaches were developed to identify plant genes which are involved in salt tolerance of plants. Here, we transformed the Arabidopsis cDNA library into a salt-sensitive calcineurin (CaN)-deficient ($cnb{\Delta}$) yeast mutant and isolated the colonies which can suppress salt-sensitive phenotype of $cnb{\Delta}$ mutant. Through this functional complementation screen, a total of 34 colonies functionally suppressed the salt-sensitive phenotype of $cnb{\Delta}$ yeast cells, and sequencing analysis revealed that these are 9 genes, including CaS, AtSUMO1 and AtHB-12. Among these genes, the ectopic expression of CaS gene increased salt tolerance in yeast, and CaS transcript was up-regulated under high salinity conditions. CaS-antisense transgenic plants showed reduced root elongation under 100 mM NaCl treatment compared to the wild type plant, which survived under 150 mM NaCl treatment, whereas CaS-antisense transgenic plant leaves turned yellow under 150 mM NaCl treatment. These results indicate that the expression of CaS gene is important for stress tolerance in yeast and plants.

Development of EST-SSR Markers and Analysis of Genetic Diversity Using Persimmon (Diospyros kaki Thunb) Cultivars Collecting from Domestic (국내 수집 감 품종을 이용한 EST-SSR marker 개발과 유전적 다양성 분석)

  • Seo, Dong Hywi;Jung, Kyung Mi;Kim, Se Jong;Kim, Kyung-Min
    • Korean Journal of Plant Resources
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    • v.26 no.4
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    • pp.491-502
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    • 2013
  • Persimmon (Diospyros kaki Thunb) fruit is one of the most important fruit and have been cultivated from ancient times in Korea. In this study, we found 16 EST-SSR markers that contained one or more EST-SSR sites from 246 cDNA sequences. The developing of EST-SSR marker analysis from 42 persimmon cultivars was compared by genetic relationships and morphological relationships using 6 qualitative traits (fruit related 6 traits) and 19 quantitative traits (flower related 19 traits). In this study, 25 primer sets were tested to identify PCR polymorphism and 14 potential EST-SSR primer pairs were selected. The result of morphological relationship EST-SSR marker analysis showed that the coefficient 0.02 was difficult to categorize in several groups. And then, coefficient 0.77 of genetic relationship showed that the group was classified as four groups. The result of correlation distance between genetic relationship and morphological relationship were investigated was low significance (-0.03). Our results also provided an optimized method for improvement of breeding efficiency and introduce of superior character at persimmon cultivars using EST-SSR markers which was useful for further investigation.

Screening of Eu3+-and Tb3+-Activated Phosphors for PDP in the System of CaO-Gd2O3-Al2O3 (CaO-Gd2O3-Al2O3계에서의 PDP용 Eu3+와 Tb3+ 활성 형광체의 탐색)

  • Park, Sang-Mi;Kim, Chang-Hae;Park, Hui-Dong;Jang, Ho-Gyeom;Park, Jun-Taek
    • Journal of the Korean Chemical Society
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    • v.46 no.4
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    • pp.336-345
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    • 2002
  • In this study, we have screened $Eu^{3+}$- and $Tb^{3+}$-activated candidate phosphors for PDP in the sys-tems of CaO-Gd$_2$O$_3$-Al$_2$O$_3$ by combinatorial chemistry and investigated the synthetic temperature, optimum com-position and luminescent properties about the candidate phosphors. To construct the emission intensity library by VUV PL, we have synthesized 210 different compositional samples using a polymerized-complex method. Good luminescent samples were identified by X-ray diffraction method. $Ca_$\alpha$$G$d_{0.95-$\alpha$-$\beta$}Al_$\beta$O_$\delta$$ : Eu(0.02< $\alpha$+$\beta$ <0.04) phos-phors screened as a red phosphor have good color purity than commercial phosphor. In the candidate phosphors of CaGdAl$_3O_7$ : Tb, Ca$Al_{12}O_{19}$ : Tb, Gd$_4$Al$_2O_9$ : Tb, and Gd$_3Al_5O_{12}$ : Tb CaGdAl$_3O_7$ : Tb, and Ca$Al_{12}O_{19}$ : Tb have shorter decay time than commercial phosphor.

An Intra Prediction Hardware Design for High Performance HEVC Encoder (고성능 HEVC 부호기를 위한 화면내 예측 하드웨어 설계)

  • Park, Seung-yong;Guard, Kanda;Ryoo, Kwang-ki
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2015.10a
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    • pp.875-878
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    • 2015
  • In this paper, we propose an intra prediction hardware architecture with less processing time, computations and reduced hardware area for a high performance HEVC encoder. The proposed intra prediction hardware architecture uses common operation units to reduce computational complexity and uses $4{\times}4$ block unit to reduce hardware area. In order to reduce operation time, common operation unit uses one operation unit to generate predicted pixels and filtered pixels in all prediction modes. Intra prediction hardware architecture introduces the $4{\times}4$ PU design processing to reduce the hardware area and uses intemal registers to support $32{\times}32$ PU processmg. The proposed hardware architecture uses ten common operation units which can reduce execution cycles of intra prediction. The proposed Intra prediction hardware architecture is designed using Verilog HDL(Hardware Description Language), and has a total of 41.5k gates in TSMC $0.13{\mu}m$ CMOS standard cell library. At 150MHz, it can support 4K UHD video encoding at 30fps in real time, and operates at a maximum of 200MHz.

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A study on vegetation and soil environmental characteristics of green roof in Daejeon Metropolitan City (대전광역시 옥상녹화 지역의 식생현황 및 토양환경 특성에 관한 연구)

  • Lee, Sang-Jin;Park, Gwan-Soo;Lee, Dong-Kun;Jang, Seong-Wan;Park, Beom-Hwan;Lee, Hang-Goo;Yun, Joon-Young;Jang, Kwan-Woo;Lee, Seung-Woo;Lee, Ho-Young;Kwon, Oh-Jung;Lee, Sook-Mee;Kil, Sung-Ho
    • Korean Journal of Agricultural Science
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    • v.38 no.4
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    • pp.641-649
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    • 2011
  • This study was to analyze the soil environmental characteristics and vegetation status of green roof in Daejeon Metropolitan City. The investigated floras of vascular plants are 17 families, 26 genera, 28 species in Seo-Gu Daejeon District Office Building (SG), 25 families, 49 genera, 56 species in Galma Public Library (GP), and 34 families, 57 genera, 60 species in Daejeon City Hall (DC) respectively. Although the larger area shows the more numbers of species in introduced plants and naturalized plant, the naturalized plant ratios were similar with each other. They were 10.71%, 10.71%, and 11.67% at SG, GP, and DC respectively. As a result of analysis on soil physical property, soil depths including vegetation soil and drainage soil of 3 green roofs were 30cm. The depths of vegetation soil at SG, GP, and DC were 0~8cm, 0~10cm, 0~10cm respectively. As a results of soil chemical properties of our study, soil pH of vegetation soil and drainage soil were a range of 6.42 and 7.43, and a range of 6.55 and 7.43 on the average respectively. Available-P contents of vegetation soil and drainage soil were a range of 153.33 and 366.33mg/kg, and a range of 136.67 and 242.67 mg/kg which is very high, respectively. Carbon contents in soil at vegetation soil and drainage soil were a range of 3.16 and 6.38%, and a range of 1.63 and 2.47% respectively. Carbon storage per square meter within 30 cm were 2.76 kg, 2.99 kg, and 3.66 kg at SG, GP, and DC respectively.

Ex Vivo MR Diffusion Coefficient Measurement of Human Gastric Tissue (인체의 위 조직 시료에서 자기공명영상장치를 이용한 확산계수 측정에 대한 기초 연구)

  • Mun Chi-Woong;Choi, Ki-Sueng;Nana Roger;Hu, Xiaoping P.;Yang, Young-Il;Chang Hee-Kyung;Eun, Choong-Ki
    • Journal of Biomedical Engineering Research
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    • v.27 no.5
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    • pp.203-209
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    • 2006
  • The aim of this study is to investigate the feasibility of ex vivo MR diffusion tensor imaging technique in order to observe the diffusion-contrast characteristics of human gastric tissues. On normal and pathologic gastric tissues, which have been fixed in a polycarbonate plastic tube filled with 10% formalin solution, laboratory made 3D diffusion tensor Turbo FLASH pulse sequence was used to obtain high resolution MR images with voxel size of $0.5{\times}0.5{\times}0.5mm^3\;using\;64{\times}32{\times}32mm^3$ field of view in conjunction with an acquisition matrix of $128{\times}64{\times}64$. Diffusion weighted- gradient pulses were employed with b values of 0 and $600s/mm^2$ in 6 orientations. The sequence was implemented on a clinical 3.0-T MRI scanner(Siemens, Erlangen, Germany) with a home-made quadrature-typed birdcage Tx/Rx rf coil for small specimen. Diffusion tensor values in each pixel were calculated using linear algebra and singular value decomposition(SVD) algorithm. Apparent diffusion coefficient(ADC) and fractional anisotropy(FA) map were also obtained from diffusion tensor data to compare pixel intensities between normal and abnormal gastric tissues. The processing software was developed by authors using Visual C++(Microsoft, WA, U.S.A.) and mathematical/statistical library of GNUwin32(Free Software Foundation). This study shows that 3D diffusion tensor Turbo FLASH sequence is useful to resolve fine micro-structures of gastric tissue and both ADC and FA values in normal gastric tissue are higher than those in abnormal tissue. Authors expect that this study also represents another possibility of gastric carcinoma detection by visualizing diffusion characteristics of proton spins in the gastric tissues.

Implementation of a pipelined Scalar Multiplier using Extended Euclid Algorithm for Elliptic Curve Cryptography(ECC) (확장 유클리드 알고리즘을 이용한 파이프라인 구조의 타원곡선 암호용 스칼라 곱셈기 구현)

  • 김종만;김영필;정용진
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.11 no.5
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    • pp.17-30
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    • 2001
  • In this paper, we implemented a scalar multiplier needed at an elliptic curve cryptosystem over standard basis in $GF(2^{163})$. The scalar multiplier consists of a radix-16 finite field serial multiplier and a finite field inverter with some control logics. The main contribution is to develop a new fast finite field inverter, which made it possible to avoid time consuming iterations of finite field multiplication. We used an algorithmic transformation technique to obtain a data-independent computational structure of the Extended Euclid GCD algorithm. The finite field multiplier and inverter shown in this paper have regular structure so that they can be easily extended to larger word size. Moreover they can achieve 100% throughput using the pipelining. Our new scalar multiplier is synthesized using Hyundai Electronics 0.6$\mu\textrm{m}$ CMOS library, and maximum operating frequency is estimated about 140MHz. The resulting data processing performance is 64Kbps, that is it takes 2.53ms to process a 163-bit data frame. We assure that this performance is enough to be used for digital signature, encryption & decryption and key exchange in real time embedded-processor environments.

Characterization of Isoflavones from Seed of Selected Soybean (Glycine max L.) Resources Using High-Resolution Mass Spectrometry (고해상도 질량 분석을 이용한 대두(Glycine max L.) 우수자원 종자의 이소플라본 특성 평가)

  • Lee, So-Jeong;Kim, Heon-Woong;Lee, Suji;Na, Hyemin;Kwon, Ryeong Ha;Kim, Ju Hyung;Yoon, Hyemyeong;Choi, Yu-Mi;Wee, Chi-Do;Yoo, Seon Mi;Lee, Sang Hoon
    • The Korean Journal of Food And Nutrition
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    • v.33 no.6
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    • pp.655-665
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    • 2020
  • In this study, chemical information on a total of 20 individual compounds was constructed to identify isoflavones from the previous reports related with used parts(seeds, leaves, stems, pods) and products of soybean(Glycine max L.). Through constructed library and UPLC-DAD-QToF/MS analysis, a total of 19 individual isoflavones including aglycones, glucosides, acetylglucosides and malonylglucosides as major compounds was identified and quantified from 14 selected soybean seeds. Among them, genistein 7-O-(2"-O-apiosyl)glucoside and genistein 7-O-(6"-O-apiosyl)glucoside(ambocin) were identified tentatively as novel compounds in soybean seeds. Besides, among malonylglucosides, glycitein 4'-O-(6"-O-malonyl)glucoside was estimated for the first time. Total isoflavone contents were distributed from 240.21 to 445.21(mg/100 g, dry matter) and 7-O-6"-O-malonylglucosides were composed of 77.8% on total isoflavone as well as genistein derivatives were confirmed as major class. It was considered importantly that the development of isoflavone-rich varieties was necessary to strengthen their effects such as anti-inflammation, anti-cancer and menopause mitigation. The qualitative and quantitative data presented precisely in this study could be help to select and breed isoflavone-rich varieties. Furthermore, their basic isoflavone profile is expected to be applied to estimate the change of isoflavone conjugates on bioavailability after soy food supplements.

A Novel Pyrazolo[3,4-d]pyrimidine Induces Heme Oxygenase-1 and Exerts Anti-Inflammatory and Neuroprotective Effects

  • Lee, Ji Ae;Kwon, Young-Won;Kim, Hye Ri;Shin, Nari;Son, Hyo Jin;Cheong, Chan Seong;Kim, Dong Jin;Hwang, Onyou
    • Molecules and Cells
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    • v.45 no.3
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    • pp.134-147
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    • 2022
  • The anti-oxidant enzyme heme oxygenase-1 (HO-1) is known to exert anti-inflammatory effects. From a library of pyrazolo[3,4-d]pyrimidines, we identified a novel compound KKC080096 that upregulated HO-1 at the mRNA and protein levels in microglial BV-2 cells. KKC080096 exhibited anti-inflammatory effects via suppressing nitric oxide, interleukin1β (IL-1β), and iNOS production in lipopolysaccharide (LPS)-challenged cells. It inhibited the phosphorylation of IKK and MAP kinases (p38, JNK, ERK), which trigger inflammatory signaling, and whose activities are inhibited by HO-1. Further, KKC080096 upregulated anti-inflammatory marker (Arg1, YM1, CD206, IL-10, transforming growth factor-β [TGF-β]) expression. In 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridinetreated mice, KKC080096 lowered microglial activation, protected the nigral dopaminergic neurons, and nigral damage-associated motor deficits. Next, we elucidated the mechanisms by which KKC080096 upregulated HO-1. KKC080096 induced the phosphorylation of AMPK and its known upstream kinases LKB1 and CaMKKbeta, and pharmacological inhibition of AMPK activity reduced the effects of KKC080096 on HO-1 expression and LPS-induced NO generation, suggesting that KKC080096-induced HO-1 upregulation involves LKB1/AMPK and CaMKKbeta/AMPK pathway activation. Further, KKC080096 caused an increase in cellular Nrf2 level, bound to Keap1 (Nrf2 inhibitor protein) with high affinity, and blocked Keap1-Nrf2 interaction. This Nrf2 activation resulted in concurrent induction of HO-1 and other Nrf2-targeted antioxidant enzymes in BV-2 and in dopaminergic CATH.a cells. These results indicate that KKC080096 is a potential therapeutic for oxidative stress-and inflammation-related neurodegenerative disorders such as Parkinson's disease.

The Mirror-based real-time dynamic projection mapping design and dynamic object detection system research (미러 방식의 실시간 동적 프로젝션 매핑 설계 및 동적 사물 검출 시스템 연구)

  • Soe-Young Ahn;Bum-Suk Seo;Sung Dae Hong
    • Journal of Internet of Things and Convergence
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    • v.10 no.2
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    • pp.85-91
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    • 2024
  • In this paper, we studied projection mapping, which is being utilized as a digital canvas beyond space and time for theme parks, mega events, and exhibition performances. Since the existing projection technology used for fixed objects has the limitation that it is difficult to map moving objects in terms of utilization, it is urgent to develop a technology that can track and map moving objects and a real-time dynamic projection mapping system based on dynamically moving objects so that it can respond to various markets such as performances, exhibitions, and theme parks. In this paper, we propose a system that can track real-time objects in real time and eliminate the delay phenomenon by developing hardware and performing high-speed image processing. Specifically, we develop a real-time object image analysis and projection focusing control unit, an integrated operating system for a real-time object tracking system, and an image processing library for projection mapping. This research is expected to have a wide range of applications in the technology-intensive industry that utilizes real-time vision machine-based detection technology, as well as in the industry where cutting-edge science and technology are converged and produced.