• 생약학회지
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• 제41권2호
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• pp.97-102
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• 2010

Six triterpenoid compounds, friedelin (1), $3{\alpha}$-hydroxy-2-friedelanone (2), canophyllol (3), oleanolic aldehyde acetate (4), ursolic acid (5), and pachysandiol A (6) were isolated from the methylene chloride soluble fraction of the roots of A. japonica. The chemical structures of compounds 1-6 were determined by the basis of physico-chemical properties and spectroscopic methods such as 1D and 2D NMR. These compounds were isolated from this plant for the first time. For the isolated compounds (1-3), the inhibitory effect of IL-6 production in TNF-$\alpha$ stimulated MG-63 was examined. Among the isolates, $3{\alpha}$-hydroxy-2-friedelanone (2) showed potent inhibitory effect on IL-6 production in TNF-$3{\alpha}$ stimulated MG-63.

• Archives of Pharmacal Research
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• 제21권1호
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• pp.12-16
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• 1998

The inhibitory effect of 10 lignan constituents isolated from the rhizomes of Coptis japonica var. dissects on tumor necrosis factor (TNF)-${\alpha}$ production in lipopolysaccharide (LPS)-stimulated macrophage cell line (RAW264.7 cells) has been studied. Among them, pinoresinol, woorenoside-V and lariciresinol glycoside showed significant inhibitory activities in the range from 37% to 55% at the concentration of $25{\mu}g/ml.$ The results are first report that the lignans isolated from Coptis japonica inhibit TNF- ${\alpha}$${\alpha}$

• IMMUNE NETWORK
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• 제11권1호
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• pp.42-49
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• 2011

Background: In this study, we have investigated the effect of Korean red ginseng (KRG) extracts on the production of TNF-${\alpha}$ and IL-8 in human keratinocytes. Also, to examine the antioxidative effect of red ginseng extracts, free radical scavenging activity and superoxide dismutase (SOD) activity in human dermal fibroblasts was measured. Methods: To investigate the effect of KRG in atopic dermatitis, we measured the level of TNF-${\alpha}$ and IL-8 secretion in LPS-stimulated human keratinocytes after the treatment of KRG extracts using enzyme-linked immunosorbent assay. Anti-oxidative activity was investigated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and SOD activity. Results: The stimulation of human keratinocytes with KRG extracts shifted the LPS-induced cytokine secretion toward a more immunosuppressive response. KRG dose-dependently decreased TNF-${\alpha}$ and IL-8 production in HaCaT cells and a significant inhibition of TNF-${\alpha}$ was shown when cells were treated with 500 and $1,000{\mu}g/ml$ of KRG extracts. Additionally, KRG extracts showed DPPH radical scavenging and SOD activity in a dose-dependent manner. Particularly, SOD activities of concentrations higher than $60{\mu}g/ml$ of KRG extracts were significantly different in human dermal fibroblast cells. Conclusion: Based on this study, KRG extracts may be a useful immunosuppressive agent in the treatment of atopic dermatitis.

• 한국응용과학기술학회지
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• 제6권1호
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• pp.51-57
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• 1989

A series of four sodium ${\alpha}-sulfo$ fatty vinyl ester oligomers including sodium ${\alpha}-sulfo$ lauric acid vinyl ester oligomer, sodium ${\alpha}-sulfo$ myristic acid vinyl ester oligomer, sodium ${\alpha}-sulfo$ palmitic acid vinyl ester oligomer and sodium ${\alpha}-sulfo$ stearic acid vinyl ester oligomer were examined for surface activities such as surface tension, foaming power, foam stability, emulsifying power, dispersion effect, solubilization of orange OT. The critical micelle concentration(CMC) was also evaluated. Consequently, these sodium ${\alpha}-sulfo$ fatty acid vinyl ester oligomers were shown to have a good cohesive power and dispersion effect.

• Archives of Pharmacal Research
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• 제26권9호
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• pp.731-734
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• 2003

Five known kaurane type diterpenoids, 16$\alpha$H, 17-isovaleryloxy-ent-kauran-19-oic acid (1), 16$\alpha$-hydroxy-17-isovaleryloxy-ent-kauran-19-oic acid (2), paniculoside-IV (3), 16$\alpha$-hydroxy-ent-kauran-19-oic acid (4), and ent-kaur-16-en-19-oic acid (5) were isolated from the root of Acanthopanax koreanum by repeated column chromatography and reversed phase preparative HPLC. The structures of these compounds were established from physicochemical and spectral data. Among the isolated compounds 16$\alpha$H, 17-isovaleryloxy-ent-kauran-19-oic acid (1) showed potent inhibitory activity ($IC_50$ value, 16.2 $\mu$ M) on TNF-$\alpha$ secretion from HMC-1, a trypsin-stimulated human leukemic mast cell line.

• 한국발생생물학회지:발생과생식
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• 제23권2호
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• pp.111-117
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• 2019

This study focused on the association of prostaglandins and a progestin, $17{\alpha}$, $20{\beta}P$-dihydroxy-4-pregnen-3-one ($17{\alpha}20{\beta}P$) during the ovulation process in longchin goby, Chasmichthys dolichognathus. We performed several in vitro experiments using $850-920{\mu}m$ diameter oocytes which were at the migratory nucleus stage. With the $890-920{\mu}m$ diameter oocytes, no significant difference in ovulation was observed in any of the prostaglandins (PGE1, PGE2, and $PGF2{\alpha}$) treated groups although PGE2 and $PGF2{\alpha}$ at concentrations of 50 ng/mL increased ovulation slightly compared with controls; however, $17{\alpha}20{\beta}P$ production was stimulated with PGE1 alone at low concentrations (5 ng/mL). In $850{\mu}m$ diameter oocytes, $PGF2{\alpha}$ at concentrations of 50 and 500 ng/mL resulted in a significant increase in ovulation. $17{\alpha}20{\beta}P$ (50 ng/mL) alone had no observable effect on ovulation, but in the combined of $PGF2{\alpha}$ 50 or 500 ng/mL it caused the greatest effect on ovulation. The sensitivity of oocytes to the induction of ovulation varies between 850 and $890-920{\mu}m$, it appeared to vary depending on the migration status of nucleus. These results suggest that $PGF2{\alpha}$ (or combined of $17{\alpha}20{\beta}P$) was more potent in inducing ovulation of the longchin goby.

• 한국자기학회:학술대회 개요집
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• 한국자기학회 2001년도 추계연구발표회 논문개요집
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• pp.44-45
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• 2001

• 한국식품과학회지
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• 제37권5호
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• pp.763-767
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• 2005

본 연구에서는 곰팡이과 세균에서 유래된 ${\alpha}-amylase$와 유화제인 monogrlyceride(MG), sodium stearoyl-2-laclylate(SSL) 및 diacetyltartaric acid ester of monol and diglycerides(DATEM)을 첨가하여 반죽을 제조하고, 제빵 반죽의 물리적 특성인 falling number, farinogram, alvogram 및 RVA를 이용한 호화특성을 살펴보았다. ${\alpha}-amylase$의 전분분해로 인하여 falling number는 감소하였으나, MG를 혼합한 반죽의 경우 전분과 높은 결합력으로 falling number가 높게 나타났다. Farinogram 특성에서는 곰팡이와 세균에서 유래된 ${\alpha}-amylase$와 유화제가 반죽의 점탄성과 흡수율, 탄력도를 감소시키고, 흡수시간은 증가시켰다. 다만 유화세로 SSL+MG를 사용하였을 때, 대조구와 유사하거나 탄력도와 흡수시간이 더 높게 나타났다. Alveogram 특성에서는 farinogram과 유사하게 $P_{max}$ 값을 감소시켜 탄력도를 감소시켰으며, 신장성, 팽창성 및 탄력에 대한 저항성을 감소시켰다. 반죽의 호화특성에서는 호화개시온도에서는 ${\alpha}-amylase$와 유화제가 영향을 주지 않았으나, ${\alpha}-amylase$의 전분 분해로 인하여 반죽의 점도를 낮추는 경향을 보여주었다.

• 대한방사선기술학회지:방사선기술과학
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• 제46권5호
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• pp.379-394
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• 2023

Targeted Alpha Therapy (TAT) is a new method of cancer treatment that protects normal tissues while selectively killing tumor cells using high cytotoxicity and short range of alpha particles, and target alpha therapy is a highly specific and effective cancer treatment strategy, and its potential has been proven through many clinical and experimental studies. This treatment method accurately delivers alpha particles by selecting specific molecules present in cancer tissue, which has an effective destruction and tumor suppression effect on cancer cells, and one of the main advantages of target alpha treatment is the physical properties of alpha particles. Alpha particles have a very high energy and short effective distance, interacting with target molecules in cancer tissues and having a fatal effect on cancer cells, which is known to cause DNA damage and cell death in cancer cells. TAT has shown positive results in preclinical and clinical studies for various types of cancers, especially those that resist or are unresponsive to existing treatments, but there are several challenges and limitations to overcome for successful clinical transition and application. These include the provision and production of suitable alpha radioisotopes, optimization of target vectors and delivery formulations, understanding and regulation of radiological effects, accurate dosage calculation and toxicity assessment. Future research should focus on developing new or improved isotopes, target vectors, transfer formulations, radiobiological models, combination strategies, imaging techniques, etc. for TAT. In addition, TAT has the potential to improve the quality of life and survival of cancer patients due to the possibility of a new treatment for overcoming cancer, and to this end, prospective research on more carcinomas and more diverse patient groups is needed.

• 대한약리학회지
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• 제20권1호
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• pp.55-65
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• 1984

[$PGF_{2{\alpha}}$]의 rat적출심방에 대한 작용을 ouabain의 작용과 비교하여 다음과 같은 결과를 얻었다. $PGF_{2{\alpha}}$의 양성변력성작용은 ouabain의 작용에 비해 potency가 강하고 efficacy는 낮았다. Ouabain은 $PGF_{2{\alpha}}$의 양성변시성작용과 유사한 양성변시성 경향을 나타내었는데 이 점에 대해 추후의 탐구가 요구된다. $PGF_{2{\alpha}}(3{\pm}10^{-8}M)$의 작용은 저칼슘농도(1.4mM)에서 ouabain$(3{\pm}10^{-3}M)$보다 현저히 $({\leq}0.05{\sim}p{\leq}0.00)$강하게 나타났고, medium내에 첨가되는 $Ca^{++}$에 대하여도 보다 예민하게 반응하였다. 저칼륨 medium(2.8mM) 또는 medium내 $K^+$첨가는 $PGF_{2{\alpha}}$보다 ouabain의 작용에 더 큰 영향을 미쳤다. $PGF_{2{\alpha}}$와 ouabain의 작용에 미치는 lidocaine ($1{\pm}10^{-5}M$이상의 고농도)의 영향은 매우 흡사하여 별다른 차이점을 볼 수 없었다. Propranolol $(3{\pm}10^{-6}M)$로써 전처치하여 아드레나린성 ${\beta}$수용체를 봉쇄한 적출심방에서 $PGF_{2{\alpha}}$ 양성변력성 및 양성변시성 작용은 방해를 받지 않았다. 이상의 결과로 보아 $PGF_{2{\alpha}}$는 ouabain의 $Na^+$, $K^+$-ATPase 억제기전파는 달리 $PGF_{2{\alpha}}$ 고유의 막수용체에 작용하여 $Ca^{++}$의 세포내 유입을 촉진시키는 기전으로 작용하는 것으로 추측되며, 그 수용체의 등정(identification)은 추후의 연구과제로 남는다.