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The Pharmaceutical Society of Korea (대한약학회)
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Inhibitory Effect of Kaurane Type Diterpenoids from Acanthopanax koreanum on TNF-$\alpha$ Secretion from Trypsin-Stimulated HMC-1 Cells

  • Cai, Xing-Fu (College of Pharmacy, Chungnam National University) ;
  • Shen, Guanghai (College of Pharmacy, Chungnam National University) ;
  • Dat, Nguyen-Tien (College of Pharmacy, Chungnam National University) ;
  • Kang, Ok-Hwa (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University) ;
  • Lee, Young-Mi (Korea Research Institute of Bioscience and Biotechnolog) ;
  • Lee, Jung-Joon (Korea Research Institute of Bioscience and Biotechnolog) ;
  • Kim, Young-Ho (College of Pharmacy, Chungnam National University)
  • Published : 2003.09.01
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Abstract

Five known kaurane type diterpenoids, 16$\alpha$H, 17-isovaleryloxy-ent-kauran-19-oic acid (1), 16$\alpha$-hydroxy-17-isovaleryloxy-ent-kauran-19-oic acid (2), paniculoside-IV (3), 16$\alpha$-hydroxy-ent-kauran-19-oic acid (4), and ent-kaur-16-en-19-oic acid (5) were isolated from the root of Acanthopanax koreanum by repeated column chromatography and reversed phase preparative HPLC. The structures of these compounds were established from physicochemical and spectral data. Among the isolated compounds 16$\alpha$H, 17-isovaleryloxy-ent-kauran-19-oic acid (1) showed potent inhibitory activity ($IC_50$ value, 16.2 $\mu$ M) on TNF-$\alpha$ secretion from HMC-1, a trypsin-stimulated human leukemic mast cell line.

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References

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