• Proceedings of the Earthquake Engineering Society of Korea Conference
• /
• 1997.04a
• /
• pp.225-232
• /
• 1997

A study of effects of design parameters on the seismic responses of base isolated structure is performed to reduce the seismic responses using a linear tw0-degree of freedom system and a lumped-mass model of a nuclear power p;ant(NPP). From the simplified 2 DOF system the optimal isolation frequency being less than 1/10th of the fundamental frequency of superstructure is obtained, and the isolator damping minimizing the peak acceleration depends on superstructure frequency. From the time history analyses for lumped mass model of NPP the optimal damping is calculated as 40% in containment building and 65% in reactor internal structure. Similar results are obtained in 2 DOF system

• Science of Emotion and Sensibility
• /
• v.15 no.2
• /
• pp.317-327
• /
• 2012

This paper uses the sensibility engineering method to find the model of automobile most appealing to buyers in the $4^{th}$ and $5^{th}$ tier cities of China. Samples of class A & A0 cars were shown to 96 subjects in those cities to investigate their responses in relation to the concepts 'modern' and 'smart'. The result shows that even though men tend to be more sensitive to automobile models in general than women are, both genders showed similar responses to specific models when they were asked to identify a model 'magnificent', 'modern', 'lively', 'smart' or 'cute'. This implies that despite the gender differences in terms of aesthetic sensitivity, both men and women share similar taste in relation to the appearance of cars. Apart from aesthetics, however, men tend to pay a greater attention to the details of design and material used for the cars, while women tend to pay attention to higher priced and better looking cars.

• Korean Journal of Child Studies
• /
• v.14 no.2
• /
• pp.35-47
• /
• 1993

The purpose of this study was to examine children's sociability as a function of sex of child, number and sex of sibling, and birth order. Children's responses were compared with mothers' responses on social participation and interpersonal adjustment. Subjects were 506 4th and 5th grade children and their mothers. Results showed that mothers' perceptions of their child's sociability were different from those of the children. 'Number of sibling' differences between only children and others were found, with onlies being most likely to show lower scores in interpersonal adjustment. Sex differences in sociability were clearly demonstrated by both children and mothers; that is, boy's interpersonal adjustment scores were lower than girls' scores. Two-way ANOVA data revealed that second-born boys and first-born girls were most likely to obtain higher scores. For siblings, elder sisters showed the highest interpersonal adjustment scores, regardless of sex of younger sibling.

• Archives of Pharmacal Research
• /
• v.29 no.11
• /
• pp.1042-1048
• /
• 2006

Plasmid DNA vaccines encoding the hepatitis B virus (HBV) surface and hepatitis C virus (HCV) envelope antigens, respectively, were constructed, and attempt were made to find the possibility of a divalent vaccine against HBV and HCV. The expression of each plasmid in Cos-1 cells was confirmed using immunocytochemistry. To measure the induced immune response by these plasmids in vivo, female BALB/c mice were immunized intramuscularly with $100\;{\mu}g$ of either both or just one of the plasmids. Anti-HBV and HCV-specific antibodies and related cytokines were evaluated to investigate the generation of both humoral and cellular immune responses. As a result, specific anti-HBV and anti-HCV serum antibodies from mice immunized with these plasmids were observed using immunoblot. The levels of IL-2 and RANTES showing a $Th_{1}$ immune response were significantly increased, but there was no change in the level of IL-4 ($Th_{1}$ immune response) in any of the immunized groups. Compared with each plasmid DNA vaccine, the combined vaccine elicited similar immune responses in both humoral and cell-mediated immunities. These results suggest that the combined DNA vaccine can induce not only comparable immunity experimentally without antigenic interference, but also humoral and $Th_{1}$ dominant cellular immune responses. Therefore, they could serve as candidates for a simultaneous bivalent vaccine against HBV and HCV infections.

• Molecules and Cells
• /
• v.44 no.5
• /
• pp.318-327
• /
• 2021

CD4+ T helper (Th) cells play a crucial role in the modulation of innate and adaptive immune responses through the differentiation of Th precursor cells into several subsets, including Th1, Th2, Th17, and regulatory T (Treg) cells. Effector Th and Treg cells are distinguished by the production of signature cytokines and are important for eliminating intracellular and extracellular pathogens and maintaining immune homeostasis. Stimulation of naive Th cells by T cell receptor and specific cytokines activates master transcription factors and induces lineage specification during the differentiation of Th cells. The master transcription factors directly activate the transcription of signature cytokine genes and also undergo post-translational modifications to fine-tune cytokine production and maintain immune balance through cross-regulation with each other. This review highlights the post-translational modifications of master transcription factors that control the differentiation of effector Th and Treg cells and provides additional insights on the immune regulation mediated by protein argininemodifying enzymes in effector Th cells.

• IMMUNE NETWORK
• /
• v.11 no.5
• /
• pp.268-280
• /
• 2011

Background: Dengue virus, which belongs to the Flavivirus genus of the Flaviviridae family, causes fatal dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) with infection risk of 2.5 billion people worldwide. However, approved vaccines are still not available. Here, we explored the immune responses induced by alternating prime-boost vaccination using DNA vaccine, adenovirus, and vaccinia virus expressing E protein of dengue virus type 2 (DenV2). Methods: Following immunization with DNA vaccine (pDE), adenovirus (rAd-E), and/or vaccinia virus (VV-E) expressing E protein, E protein-specific IgG and its isotypes were determined by conventional ELISA. Intracellular CD154 and cytokine staining was used for enumerating CD4+ T cells specific for E protein. E protein-specific CD8+ T cell responses were evaluated by in vivo CTL killing activity and intracellular IFN-${\gamma}$ staining. Results: Among three constructs, VV-E induced the most potent IgG responses, Th1-type cytokine production by stimulated CD4+ T cells, and the CD8+ T cell response. Furthermore, when the three constructs were used for alternating prime-boost vaccination, the results revealed a different pattern of CD4+ and CD8+ T cell responses. i) Priming with VV-E induced higher E-specific IgG level but it was decreased rapidly. ii) Strong CD8+ T cell responses specific for E protein were induced when VV-E was used for the priming step, and such CD8+ T cell responses were significantly boosted with pDE. iii) Priming with rAd-E induced stronger CD4+ T cell responses which subsequently boosted with pDE to a greater extent than VV-E and rAd-E. Conclusion: These results indicate that priming with live viral vector vaccines could induce different patterns of E protein-specific CD4+ and CD8+ T cell responses which were significantly enhanced by booster vaccination with the DNA vaccine. Therefore, our observation will provide valuable information for the establishment of optimal prime-boost vaccination against DenV.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.33 no.3
• /
• pp.236-241
• /
• 1988

The responses of GA$_3$ treatment on the culm length, internode elongation, number of internodes elongated and on the panicle length of 115 Japonica rice cultivars were studied. One spray with 50 ppm GA$_3$ was made at about 30 days before heading. Generally short culm cultivars showed better elongation responses than taller ones and a few taller cultivars showed reduced culm length. The internode length of 1st, 5th, 6th and 7th were increased, but, 2nd, 3rd and 4th internodes were reduced. Especially the reduction of 3rd internode elongation and the increase of 5th internode length was prominent. Most of the tested cultivars showed no changes in the number of internodes elongated, but, in some cultivars it was increased and in a few ones it was reduced. The effect on the panicle length was variable depending cultivars. No significant correlation was found between the culm length elongation and the elongation of panicle length, implying that the response of GA$_3$ on the culm and on the panicle is independent of each other. According the response of culm length elongation tested cultivars were grouped into 6 ; Significantly reducing, slightly reducing, less sensitive, slightly elongating, largely elongating and extremely elongating.

• Journal of Technologic Dentistry
• /
• v.44 no.2
• /
• pp.47-52
• /
• 2022

Purpose: This study aims to identify the recognition, readiness, and awareness of the need for education in the era of the 4th industrial revolution among dental technology students in the healthcare field, and to provide essential data for the development of a future-oriented curriculum. Methods: A survey of 260 students, who were briefed about the purpose of this study, was conducted at D University and G University, located in Daejeon and Gangwon-do, South Korea, respectively. A total of 229 questionnaires, excluding 31 with insufficient responses, were analyzed. Results: 1. Recognition of the 4th industrial revolution was 3.43±0.77, preparedness was 2.91±0.84, and awareness of the need for education was 3.86±0.80. 2. Major satisfaction was found to have a significant effect on concept recognition and impact recognition of the 4th industrial revolution, readiness level, and the need for education (p<0.001). Conclusion: It is necessary to develop an education program on specialized skills (professional knowledge) related to the 4th industrial revolution, and to implement such a program that serves the educational needs of individual groups.

• BMB Reports
• /
• v.35 no.6
• /
• pp.623-628
• /
• 2002

The Th1 vs. Th2 balance is critical for the maintenance of immune homeostasis. Therefore, the genes that are selectively-regulated by the Th1 and Th2 cytokines are likely to play an important role in the Th1 and Th2 immune responses. In order to search for and identify the novel target genes that are differentially regulated by the Th1/Th2 cytokines, the human PBMC mRNAs differentially expressed upon the stimulation with IL-4 or IL-12, were screened by employing the differential display-polymerase chain reaction. Among a number of clones selected, DC21 was identified as a novel target gene that is regulated by IL-4 and IL-12. The DC21 gene expression was up-regulated either by IL-4 or IL-12, yet counter-regulated by co-treatment with IL-4 and IL-12. DC21 is a dendritic cell protein with an unknown function. The sequence analysis and conserved-domain search revealed that it has two AU-rich motifs in the 3'UTR, which is a target site for the regulation of mRNA stability by cytokines, and that it belongs to the N-acetyltransferase family. The induction of DC21 by IL-12 peaked around 8-12 h, and lasted until 24 h. LY294002 and SB203580 significantly suppressed the IL-12-induced DC21 gene expression, which implies that PI3K and p38/JNK are involved in the IL-12 signal transduction pathway that leads to the DC21 expression. Furthermore, tissue blot data indicated that DC21 is highly expressed in tissues with specialized-resident macrophages, such as the lung, liver, kidney, and placenta. Together, these data suggest a possible role for DC21 in the differentiation and maturation of dendritic cells regulated by IL-4 and IL-12.

• IMMUNE NETWORK
• /
• v.24 no.2
• /
• pp.14.1-14.26
• /
• 2024

The inflammatory response during cutaneous leishmaniasis (CL) involves immune and non-immune cell cooperation to contain and eliminate Leishmania parasites. The orchestration of these responses is coordinated primarily by CD4⁺ T cells; however, the disease outcome depends on the Th cell predominant phenotype. Although Th1 and Th2 phenotypes are the most addressed as steers for the resolution or perpetuation of the disease, Th17 cell activities, especially IL-17 release, are recognized to be vital during CL development. Th17 cells perform vital functions during both acute and chronic phases of CL. Overall, Th17 cells induce the migration of phagocytes (neutrophils, macrophages) to the infection site and CD8⁺ T cells and NK cell activation. They also provoke granzyme and perforin secretion from CD8⁺ T cells, macrophage differentiation towards an M2 phenotype, and expansion of B and Treg cells. Likewise, immune cells from the inflammatory infiltrate have modulatory activities over Th17 cells involving their differentiation from naive CD4⁺ T cells and further expansion by generating a microenvironment rich in optimal cytokines such as IL-1β, TGF-β, IL-6, and IL-21. Th17 cell activities and synergies are crucial for the resistance of the infection during the early and acute stages; however, if unchecked, Th17 cells might lead to a chronic stage. This review discusses the synergies between Th17 cells and the inflammatory infiltrate and how these interactions might destine the course of CL.