• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제30권5호
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• pp.380-390
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• 2004

Bone healing plays an important role in orthognathic and craniofacial surgery. Bone tissue repair and regeneration are regulated by an array of growth and morphogenetic factors. Bone formation and remodeling require continuous generation of osteoprogenitor cells from bone marrow stromal cells, which generate and respond to a variety of growth factors with putative roles in hematopoiesis and mesenchymal differentiation. In this study, the efficacy of a single application of hepatocyte growth factor to promote bone regeneration in 5-mm experimental calvarial defects of adult male rats was assessed histologically and immunohistochemically. The result of the experimental site were compared with those of the contralateral contral side. None of the control and experimental bone defects demonstrated complete bone closure. Bone regeneration was found close th the margine and central part of the defects. At 1, 2 weeks, there were found much significant cellural mitotic activity and many inflammatory cells and osteoblasts on the experimental site than control site. At 4, 6 weeks, new bone apposition was founded in both site but, more apposition was seen at experimental site. At 8, 12 weeks, also, some differences was found that more apposition of new bone and collagen fiber was seen on experimental site. Our results have some possibility that HGF do a early positive role to repair the bone defect. More study will be needed.

• 대한한의학원전학회지
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• 제22권2호
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• pp.85-99
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• 2009

This book was completed in the 33rd year of Gang-hui-gapsul(康熙 甲戌) in the Cheong(淸) dynasty(1694), and was first inscribed in the 55th year of Geonryung(乾隆). In this first edition named 'Daechudangbon(大雅堂本)', his descendant Jinbonghui(陳鳳輝) wrote the postscript. There are also other editions such as 'Ga-gyeong-ganchwihyeondangbon(嘉慶間聚賢堂本)', 'Wimundanggeonsangbon(緯文堂巾箱本)' and 'Guangseo-ganseonseongdangbon(光緒間善成堂本)' Second, this book was called "Oe-gwabirok(外科秘錄)" and used the pen name 'Gibaekchunsasojeon(岐伯天師所傳)'. There are 16 volumes in total. The beginning of volume one, is a drawing of the 14 meridians. Volumes 1$\sim$4 are on the symptoms and treatment of abscesses and sores and ulcers[癰疽瘡瘍]. Volumes 5$\sim$13 are on surgery, dermatology and 156 diseases such as wounds by contusion, sharp objects and insects and beasts[跌撲, 金刃, 蟲獸傷]. Volumes 14$\sim$16 list the internal treatments, external medicine, acupuncture and moxibustion and surgery of sores and ulcers. Third, the book stressed early detection and treatment of diseases, emphasized inner resolving[內消] being cautious about using medicine, and further deepened the syndrome differentiation and treatment[辨證施治] of sores and ulcers[瘡瘍] by dividing fire toxin(火毒) into Eumhwa and Yanghwa(陰火/陽火). Also, it established the cause of sores and ulcers development as the deficiency of Gi(氣) and blood[血] and focused especially on the liver and kidney's involvement in the process. It also asserted that the pathogen[邪] is the tip[標], so one should eliminate and reinforce[攻補] to balance it out. The sore and ulcer surgery[瘡瘍外科] part is mostly based on detoxifying[解毒] and resolving[消散]. Although the meridians were mentioned, they were not emphasized, while moxibustion treatment of sores and ulcers[瘡瘍] were thoroughly analyzed. Also, of all 550 formulas in this book, 90% are experience-based which frequently use Geum-eunhwa(金銀花), Pogong-yeong(蒲公英) and Jahwajijeong(紫花地丁). The usages are unique, and the formulas have good adaptability. The symptoms of the diseases are explained first, followed by according main and sub treatments.

• 복식문화연구
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• 제17권4호
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• pp.600-615
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• 2009

$21^{st}$ century post digital society orients co-existence, fusion, and blurring boundary than conflict, differentiation, and boundary, and makes a try liberal combination of various different objects. Furthermore, radical development of science and digital equipments offer technical possibility that could combine various fields. Hence, many different departments demolish their boundary, and combine for development of multi-functional and complex shape's products. For job-nomads, fashion attempts to combine with architecture, furniture, daily necessities, and digital equipments spontaneously. This paper aims at consideration about the blurring phenomena expressed in complex fashion space of $21^{st}$ century throughout empirical fashion photographs analysis, which show combination among fashion and various different fields. Blurring boundary phenomena of complex fashion space are classified with 4 parts as follows as: 1) furniturizing, 2) wearable dwelling, 3) lumiduct, 4) becoming fashion. Each parts are examined 8 aesthetical characteristics such as movement and lightness, hyper-link and openness, immateriality and inter-activeness, and diversity and ambiguity. $21^{st}$ century fashion has changed more simple and light, and creates new form throughout combination with many other fields, and enlarges its function and sphere. I think this paper would help certificating practical use of fashion space as multiple and complex space, and makes contribution to forecast about fashion development of the future and offer inspiration about creative and innovative fashion design.

• 한국수정란이식학회:학술대회논문집
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• 한국수정란이식학회 2002년도 국제심포지엄
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• pp.97-97
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• 2002

Human eryhropoietin (EPO) is acidic glycoprotein hormone that plays key role in hematopoiesis by facilitating differentiation of erythrocyte and formation of hemoglobin (Hb) and is used for the treatment of anemia. Human EPO is consist of 166 amino acids which is modified by three N-glycosylations (24, 38, 83) and single O-glycosylation (126). N-glycosylation is reported to be related to the cellular secretion and activity of EPO. In this study, we examined effects of mutagenesis in glycosylation site of recombinat hEPO for the cellular secretion during production from cultured CHO cell. We produced rhEpo which was cloned by PCR from human liver cDNA (TaKaRa) in cultured CHO cell. Using supernatant of the culture, ELISA assay and western analysis were performed. To estimate biological activity, 20IU of rhuEpo was subcutaneously injected into four ICR mice. After 8 days, HCT level was increased average 13 per cent, RBC was increased ca. 2${\times}$10$\^$6//${\mu}\ell$. In disease model Rat (anemia c-kit, WSRC-WS/WS), HCT was increased ca. 12%, RBC was increased ca. 1.6${\times}$10$\^$6//${\mu}\ell$. These results suggests that rhEpo we produced has biological activity. To remove glycosylation site by substituting 24, 38, 83, and 126th asparagine (or serine) with glutamic acid, overlapping -extension site-directed mutagenesis was performed. To add novel glycosylation sites, 69, 105th leucine was mutated to asparagine. Mutant EPO construct was transfected into CHO cell. Supernatant of the cell culture was analyzed using ELISA assay with monoclonal anti-EPO antibody (Medac, Germany). Since, several reports for mutagenesis of glycosylation sites showed case-by-case results, we examined both transient expression and stable expression. Addition of novel glycosylation sites resulted no secretion while deletion mutants had little effect except some double deletion mutants (24/83 and 38/83) and triple mutant. We suggest that not single but combination of glycosyl group affect secretion of EPO.

• 한국지역지리학회지
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• 제5권2호
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• pp.47-117
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• 1999

도시에 관심을 가지는 사람이면 누구나 "도시란 무엇인가?"라는 화두(話頭)가 떠오르게 편다. 그리고 끊임없이 생각을 확장시켜 갈 것이다. 그러나 대부분은 "도시란 무엇인가?"에 대한 대답이 아니라 도시적인 현상, 도시의 특징, 농촌과의 차이점으로 사고(思考)를 진행시켜 갈 것이다. 또한 화두(話頭)가 무엇인지 망각하면서 계속해서 도시를 연구하고 또 분석할 것이다. 이렇게 화두(話頭)와 사고(思考)의 방향이 서로 엇갈리게 되는 경우가 인간의 현상에 있어서는 그리 흔하지 않을 것이다. 도시란 오묘한 존재임에는 틀림이 없다. 도시의 내부구조를 파악하기 위해서, 그리고 그 구조에 영향을 비치는 어떤 공간적인 원리, 나아가서 법칙이 존재한다는 신념을 가지고 있는 것이 도시지리학도들이다. 우리가 관심을 가지는 것은 가시적인 현상이 아니라, 그 현상 뒤에 숨어서 그 현상을 존재하도록 하는 법칙일 것이다. 어떻게 그 법칙을 추구할 수 있을까? 어떻게 하면 진리를 찾을 수 있을까? 과연 찾을 수 있을까? 지리학에 있어서 '무엇이 어디에 있는가?'하는 것은 중요하지 않다. '왜, 어떻게 있느냐'가 중요하다. 그러나 어디에 무엇이 있는지도 모르는 채 '왜', '어떻게'에 대한 정확한 해답을 얻을 수 있을까 하는 의문이 생긴다. 우리는 누적(累積)된 연구 성과의 중요성을 등한시 해서는 안된다. 선배들의 연구 업적을 간과해서도 안될 것이며, 또한 무비판적으로 수용해서도 안될 것이다. 지리학도들은 미시적(微視的)인 공간에 적용되는 원리는 거시적(巨視的)인 공간에도 적용되며, 그 역(逆)도 성립한다는 사실을 인식하고 있다. 본 연구는 미시적인 지역에 대한 접근이다. 부산시 내부에 존재하고 있는 CBD를 비롯한 여러 계층의 중심지(상점가)에 대한 접근의 일환으로 이루어지고 있다. 최종적으로는 부산시 전체의 공간구조를 파악하기 위한 목적을 염두에 두고 지난 10여년간 단계적으로 행하여 온 연구의 한 부분의 성격을 지니고 있다. 부산역 상점가는 부산시 내부에서는 온천장 상점가와 더불어 오히려 특수 기능 상점가의 하나이다. 이곳에 대해서 본 논문에서는 기능별 패턴의 일부, 층별 규모별 패턴, comer의 패턴, 가로별 패턴을 분석하여 부산역 상점가에 대한 접근을 완성하였다.

• 생명과학회지
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• 제16권6호
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• pp.904-910
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• 2006

수지상세포는 종양면역에서 필수적인 강력한 CTL 반응을 개시할 수 있는 유일한 세포이다 . 특히 외인성 종양항원에 대한 CTL 반응 유도는 활성화된 수지상세포의 IL-12 분비를 통한 CD4+ helper T세포의 cross-priming을 필요로 한다. 그러나 최근에 활성화된 수지상세포는 $Th_1$ 면역반응을 유도하지만 활성화 시간이 경과함에 따라 오히려 $Th_2$반응을 유도 할 수 있다. 따라서 본 연구에서는 OVA를 종양항원 모델로 설정하여 종양특이적인 CTL 반응을 형성하기 위한 최적의 수지상세포 활성화 조건을 조사하였다. 마우스 골수세포에 서 수지상세포로의 분화는 항원제시 기능을 위한 표면분자의 발현 측면에서 볼 때 배양 6일-7일 정도가 적합하였다. 수지상세포의 IL-12 생성능은 배양 6일 이상, OVA 항원 탑재 8시간 이상의 경우에 연이은 LPS 성숙자극으로 오히려 감소하는 경향을 보였다. 즉 배양 6일의 수지상세포에 OVA 항원 탑재를 8시간 수행한 경우(8-h DC)가 in vitro에서의 IL-12생성능, ex vivo에서의 세포내 $IFN-{\gamma}$를 발현하는 CD8+ T세포의 증가 및 OVA 특이적인 세포독성효과 등에서 가장 좋은 결과를 보였다. 또한 in vivo에서 종양 치료 및 예방효과에서도 8-h DC로 면역한 경우에 가장 우수한 종양형성 억제 효과와 생존기간 연장효과를 보였다. 현재 대부분의 수지상 세포를 이용한 항종양 백신에서 항원 탑재반응을 24시간 동안 수행하고 있으나, 본 실험의 결과로 볼 때, 8시간의 in vitro 항원 탑재가 보다 효과적인 종양특이적 CTL 반응과 항종양 면역반응을 유도함을 알 수 있다. 결론적으로 본 연구를 통하여 8시간 이상의 항원접촉은 수지상세포의 기능적 활성능력을 오히려 고갈시킬 수 있음을 제시한다.

• 대한한의학원전학회지
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• 제22권2호
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• pp.71-83
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• 2009

The documentary characteristics of acupuncture and moxibustion recorded in Dusagyeong(杜思敬)'s".Jesaengbalsu(濟生拔粹)" can be summarized into 3 major parts: 1. "Gyeolgo-ungichimbeop(潔古雲岐鍼法)" and "Dutaesachimbeop(竇太師鍼法)" 1) "Gyeolgo-ungichimbeop" was edited by Dusagyeong of the Won dynasty, and was recorded in "Jesaengbalsu". Du was influenced by his teacher Heohyeong(許衡) and followed Janggyeolgo(張潔古) and his son Jangbyeok(張璧), and collected his work "Chimgu-pyeon(鍼灸篇)" for Jang and named it "Gyeolgo-ungichimbeop", and took the content from the medical book of Jang and his student Wang-haejang(王海藏). (2) "Jesaengbalsu"'s original edition exists today. The "Gyeolgo-ungichimbeop" listed in "Jesaengbalsu"'s index contain two collections, the first collection being "Gyeolgo-ungichimbeop" and the second collection being "Dutaesachimbeop(竇太師鍼法)" (3) Gyeolgo(潔古)、Un-gija(雲岐子)'s acupuncture methods can be seen in Un-gija "Bomyeongjipryuyo(保命集類要)" and Wanghaejang "Chasananji(此事難知)". (4) The related acupuncture methods are 'Non-gyeong-rak-yeongsubosabeop(論經絡迎隨補瀉法)', 'Gyeong-rakchwiwonbeop(經絡取原法)', 'Jeopgyeongbeop(接經法)', and 'Sang-hanyeolbyeongjabeop(傷寒熱病刺法)' (5) Du's edition of the entire text of 'Gyeolgojajetongbeop(潔古刺諸痛法)' 'Jasimtongjehyeol(刺心痛諸穴)' and the first half of 'Jeopgyeongbeop(接經法)' is all recorded in "Somunbyeonggigi-uibomyeongjip(素問病機氣宜保命集)". The existing "Somunbyeonggigi-uibomyeongjip" is a combination of the unfinished posthumous work of Yuwanso(劉完素), "Gi-ui(氣宜)" and "Byeonggi(病機)" with works such as Jangwonso(張元素)'s '"Bomyeongseo(保命書)"'. (6) Of the titles "Gyeolgo-ungichimbeop" and "Dutaesachimbeop", the 14$\sim$19th chapters "Dutaesachimbeop" should be concentrated at the end of the chapter, and the 16th chapter that Du added was put after chapter 14 "Yujujiyobu(流注指要賦)", and chapters 20, 21 should be put in "Gyeolgoungichimbeop" after chapter 13. 2. "Chimgyeongjeok-yeongjip(鍼經摘英集)" (1) "Chimgyeongjeok-yeongjip" is a collection of the acupuncture and moxibustion contents of medical books from the Geum and Won dynasties that Dusagyeong collected and organized during the Won dynasty, which is consisted of 5 chapters : "Guchimshik(九鍼式)", "Jeolyangchwisuhyeolbeop(折量取腧穴法)", "Bosabeop(補瀉法)", "Yongchimhoheupbeop(用鍼呼吸法)", "Chibyeongjik-ralgyeol(治病直剌訣)". (2) First, the contents. The nine acupuncture needles[九鍼] listed in "Guchimshik(九鍼式)" is the first existing document recording to systematically illustrate the 'nine classical needles' in drawing and text form which reflects the forms of the needles of the era. Second, "Jeolyangchwisuhyeolbeop(折量取腧穴法)" has the same basic way of measuring points [量穴法] as Wang-yuil's "Dong-insuhyeolchimgudo-gyeong(銅人腧穴鍼灸圖經)" and the same point selection rules as "Jeonyeongbang(全嬰方)". Third, in "Bosabeop(補瀉法)", "Somun(素問)" and Janggyeolgo's "Yeongsubosabeop(迎隨補瀉法)" is put together. Fourth, in "Yongchimhoheupbeop(用鍼呼吸法)", the cold and heat supplementation and draining [寒熱補瀉] method that combines breathing with inner and outer rotation[外 內撚] is recorded. Fifth, "Chi-byeongjik-ralgyeol(治病直剌訣)" is the main part of "Chimgyeongjeok-yeongjip(鍼經摘英集)" listing 69 acupuncture treatments reflecting Du's scholastic ideas on aspects such as syndrome differentiation[辨證], needling method and type of needle[鍼具]. (3) The content of this book was quoted by "Bojebang Chimgumun(普濟方 鍼灸門)" and when Gomu compiled "Chimguchwiyeong", he put the acupuncture treatments for the main indications of the disease patterns[鍼方主治病證] of this book in the related main indications of acupuncture points[腧穴主治證], which influenced books on acupuncture points there after. 3. "Chimgyeongjeolyo(鍼經節要)" (1) Consists of 1 volume. The original title of this book is "Dong-insuhyeolchimgudo-gyeong (銅人腧穴鍼灸圖經)" and the author is Wang-yuil of the Northern Song dynasty, written in the 4th year of the Cheonseong(天聖) era of the Song dynasty(1026). (2) Dusagyeong selected the contents on pathology of the 12 meridians in volume one and two, the introduction and five transport points[五輸穴] in volume 5 of "Dong-indo-gyeong(銅人圖經)" and named it "Chimgyeongjeolyo." During the Won dynasty it was recorded in "Jesaengbalsu".

• 아동학회지
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• 제1권
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• pp.15-27
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• 1980

PURPOSE This study was intended to analyze the play-form of normal preschool children as related to age, sex, educational experience and social maturity. METHOD 1. Subjects The subjects of this study were eighty seven 3-, 4- and 5-year-old preschool children attending educational institutions in Seoul. 2. Instruments The instruments used in this study consisted of twenty one-minute observations in 5 preschool settings and a questionnaire designed to measure the children's social maturity filled in by their parents. The observations provided information on the children's free play, that is 1) play-form: solitary, parallel and group play 2) use/non-use of toys 3) behavior: play, activity, doing nothing, onlooking 4) stationary/moving dimensions 5) play group size The questionnaire measured such factors as communication, socialization, locomotion, self-help and self-direction. 3. Data Analysis Data analysis consisted of percentage and Chi square. RESULTS 1. Play-Form The relationship between age and play-form proved to be significant at the .05 level. That is, in both boys and girls, solitary play decreased with age and group play increased with age while parallel play maintained a similar position. The relationship between sex and play-form proved to be significant at the .05 level in 3-& 4-year-old children, but not significant in 5-year-old children. That is, in their $3^{rd}$ year boys engage in the solitary play more than girls and in their $4^{th}$ year boys engage in more group play than girls. 2. Toys Without differentiation of sex and age, cases of using toys in play exceed those cases where toys were not used. 3. Behavior The relationship between age and behavior proved to be significant at the .05 level in both boys and girls. That is, with increase of age, play decreased and activity increased in boys while play increased and doing nothing decreased in girls. 4. Moving Status Totally, moving occurred more frequently in play than stationary status. Moving proved not to have a significant relationship with age, but it did Navel a significant relationship with sex at the .05 level. That is, moving tended to increase with the increase in age in both boys and girls, but not at a significant level. Boys moved significantly more than girls in all three age levels. 5. The Relationship between Educational Experience and Play-Form There was a tendency for children with longer preschool experience to engage in less solitary play and more group play than children who had less than one semester of preschool experience, but this difference was not statistically significant. 6. The Relationship between Social Maturity and Play-Form The high social maturity group engaged in less solitary play and more group play than the low social maturity group, but this was not a statistically significant difference. 7. Play Group Size Play group size was 2~10 children, but the majority of play groups consisted of 2~3 children. There was a tendency for play groups composed of 2 children to decrease with age and play groups composed of 3 children to increase with age. No significant difference was found between the sexes in play group size.

• 동의생리병리학회지
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• 제30권2호
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• pp.109-115
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• 2016

Hataedock is a Korean medical treatment that administers herbal extracts orally to newborn infants. This method is used for alleviating harmful heat and excreting fetal wastes by meconium. The purpose of this study was to evaluate anti-inflammatory effect of Hataedock method with Douchi on 2,4-dinitrofluorobenzene (DNFB)-induced atopic dermatitis (AD). The 3-week-old NC/Nga mice were divided into 3 groups: the control group (Ctrl), the AD-induced group (AE), and the Hataedock-treated group (GT). Only the GT group was treated with Hataedock at the 3rd week. After 28 days from Hataedock treatment, we induced AD-like dermatitis to the AE and GT group by DNFB. The effects of Hataedock were evaluated by immunohistochemical method. In the epithelium, PKC-positive reaction of the GT group was decreased by 57%. In the dermal papillae, IL-4-positive reaction was decreased by 34%. In the dermis, the distribution of degranulated mast cells was decreased and substance P-positive reaction was decreased by 49%. In the skin tissue, edema was decreased and MMP-9-positive reaction was decreased by 71%. Tissue damage such as epithelial cell hyperplasia, infiltration of granulocyte and lymphocyte, and capillary distribution were also decreased. The Hataedock method with Douchi maintained skin barrier and inhibited skin-damaging factors via regulating Th2 differentiation. In conclusion, Hataedock has a potential for preventative treatment of AD. Further studies are necessary to investigate the immune-regulating mechanism and verify the safety and efficacy of the Hataedock method.

• 한국산림과학회지
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• 제100권1호
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• pp.62-69
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• 2011

본 연구는 5차 국가산림자원조사(National Forest Inventory, NFI) 표본점 자료를 이용하여 충청도 지역에 분포하는 주요 수종에 대한 수고-흉고직경 생장모델을 개발하고자 하였다. 충청도 지역 고정표본지 내에서 수고와 흉고직경이 측정된 주요 수종의 총 임목 본수는 2,681본이었으며, 무작위로 생장모델의 개발을 위해 90% 자료와 모델 타당성 검정을 위해 10% 자료로 나누어서 분석하였고, 본 연구에서 제시된 최종모형의 추정된 계수는 100% 자료를 이용하였다. 8개 주요 수종에 대한 생장모델들의 적합성 검정은 결정계수($R^2$), 추정치의 오차인 평균제곱근오차(RMSE), 평균편의(MD), 절대평균편의(AMD)와 직경급별로 평균편의(MD)를 비교 분석하였다. 본 연구 결과에 의하면, 6개 주요 생장식의 결정계수는 모두 94% 이상의 높은 설명력을 나타냈으며, 특히 C-R 생장모델과 Weibull 생장모델은 다른 모델에 비해 좋은 결과를 나타냈다. 직경급 30 cm 이하에서는 소나무, 리기다소나무, 굴참나무, 신갈나무가 상대적으로 가장 작은 평균편의를 나타낸 반면, 직경급 30 cm 이상에서는 신갈나무, 상수리나무, 졸참나무가 큰 평균편의를 나타냈다. 또한 본 연구의 결과로 제시된 6개 주요 생장식에서 추정한 수고를 임목자원평가 프로그램에 적용하여 간재적을 분석한 결과, 직경급 30 cm까지는 큰 차이를 보이지 않지만, 30 cm 이상인 대경목의 경우 추정된 간재적은 큰 차이를 보이므로, 생장모델 선정에 주의를 기울여야 한다.