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http://dx.doi.org/10.15188/kjopp.2016.04.30.2.109

Effects of Hataedock with Douchi on 2,4-dinitrofluorobenzene-induced Atopic Dermatitis-like Skin Lesion in NC/Nga Mice  

Song, Ji-hoon (Department of Pediatrics, School of Korean Medicine, Pusan National University)
Ahn, Sang-Hyun (Research Institute for Korean Medicine, Pusan National University)
Cheon, Jin-Hong (Department of Pediatrics, School of Korean Medicine, Pusan National University)
Park, Sun-young (Department of Physiology, College of Korean Medicine, Semyung University)
Kim, Ho-Hyun (Department of Physiology, College of Korean Medicine, Semyung University)
Kim, Ki-Bong (Department of Pediatrics, School of Korean Medicine, Pusan National University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.30, no.2, 2016 , pp. 109-115 More about this Journal
Abstract
Hataedock is a Korean medical treatment that administers herbal extracts orally to newborn infants. This method is used for alleviating harmful heat and excreting fetal wastes by meconium. The purpose of this study was to evaluate anti-inflammatory effect of Hataedock method with Douchi on 2,4-dinitrofluorobenzene (DNFB)-induced atopic dermatitis (AD). The 3-week-old NC/Nga mice were divided into 3 groups: the control group (Ctrl), the AD-induced group (AE), and the Hataedock-treated group (GT). Only the GT group was treated with Hataedock at the 3rd week. After 28 days from Hataedock treatment, we induced AD-like dermatitis to the AE and GT group by DNFB. The effects of Hataedock were evaluated by immunohistochemical method. In the epithelium, PKC-positive reaction of the GT group was decreased by 57%. In the dermal papillae, IL-4-positive reaction was decreased by 34%. In the dermis, the distribution of degranulated mast cells was decreased and substance P-positive reaction was decreased by 49%. In the skin tissue, edema was decreased and MMP-9-positive reaction was decreased by 71%. Tissue damage such as epithelial cell hyperplasia, infiltration of granulocyte and lymphocyte, and capillary distribution were also decreased. The Hataedock method with Douchi maintained skin barrier and inhibited skin-damaging factors via regulating Th2 differentiation. In conclusion, Hataedock has a potential for preventative treatment of AD. Further studies are necessary to investigate the immune-regulating mechanism and verify the safety and efficacy of the Hataedock method.
Keywords
Hataedock(下胎毒); Douchi(豆豉); Atopic dermatitis; PKC; IL-4; NC/Nga Mice;
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