Browse > Article

THE EFFECT OF HEPATOCYTE GROWTH FACTOR FOR REPAIR OF THE BONE DEFECT  

Shin, Sang-Hun (Dept. of Oral & Maxillofacial Surgery, College of Dentistry, Pusan National University)
Kim, Chang-Ju (Dept. of Oral & Maxillofacial Surgery, College of Medicine, Dong-A University)
Kim, Cheol-Hun (Dept. of Oral & Maxillofacial Surgery, College of Medicine, Dong-A University)
Kim, Yong-Deok (Dept. of Oral & Maxillofacial Surgery, College of Dentistry, Pusan National University)
Chung, In-Kyo (Dept. of Oral & Maxillofacial Surgery, College of Dentistry, Pusan National University)
Publication Information
Journal of the Korean Association of Oral and Maxillofacial Surgeons / v.30, no.5, 2004 , pp. 380-390 More about this Journal
Abstract
Bone healing plays an important role in orthognathic and craniofacial surgery. Bone tissue repair and regeneration are regulated by an array of growth and morphogenetic factors. Bone formation and remodeling require continuous generation of osteoprogenitor cells from bone marrow stromal cells, which generate and respond to a variety of growth factors with putative roles in hematopoiesis and mesenchymal differentiation. In this study, the efficacy of a single application of hepatocyte growth factor to promote bone regeneration in 5-mm experimental calvarial defects of adult male rats was assessed histologically and immunohistochemically. The result of the experimental site were compared with those of the contralateral contral side. None of the control and experimental bone defects demonstrated complete bone closure. Bone regeneration was found close th the margine and central part of the defects. At 1, 2 weeks, there were found much significant cellural mitotic activity and many inflammatory cells and osteoblasts on the experimental site than control site. At 4, 6 weeks, new bone apposition was founded in both site but, more apposition was seen at experimental site. At 8, 12 weeks, also, some differences was found that more apposition of new bone and collagen fiber was seen on experimental site. Our results have some possibility that HGF do a early positive role to repair the bone defect. More study will be needed.
Keywords
Citations & Related Records
연도 인용수 순위
  • Reference
1 Fuller K, Ewens J, Chambers TJ: The effect of hepatocyte growth factor on the behavior of osteoclasts. Biochem Biophys Res Commun 1995;212:334   DOI   ScienceOn
2 Amano O, Koshimizu U, Nakamura T et al.: Enhancement by hepatocyte growth factor of bone and cartilage formation during embryonic mouse mandibular development in vitro. Arch Oral Biol 1999;44;935
3 G. D’ippolito, PC Schiller, C Perez-Stable: Cooperative Actions of Hepatocyte Growth Factor and 1,25-Dihydroxy vitamin D3 in Osteoblastic Differation of Human Vertebral Bone Marrow Stromal Cells. Bone 2002;31:269
4 Takayama H, LaRochelle WJ, Anver M et al.: Scatter factor/hepatocyte growth factor as a regulator of skeletal muscle and neural crest development. Proc Natl Acad Sci USA 1996;93:5866
5 Balkovota DF, Lipochutz IH: Hepatocyte growth factor and the kidney: it is just not for the liver. Int Rev Cytol 1999;188:225
6 Zarnegar R, Michalopoulos GK: The many faces of hepatocyte growth factor from hepatopoiesis. J Cell Biol 1995;129:1177   DOI   ScienceOn
7 Matsumoto K, Nakamura T: Hepatocyte growth factor (HGF) as a tissue organizer for organogenesis and regeneration. Biochem Biophys Res Commun 1997;239:639
8 Yasuda H, Imai E, Shiota A et al.: Anti-fibrogenic effect of a deletion variant of hepatocyte growth factor on liver fibrosis in rats. Hepatology 1996;24:638
9 Kosai K, Matsumoto K, Nakamura T: Hepatocyte growth factor prevents endotoxin-induced lethal hepatic failure in mice. Hepatology 1999;30:151
10 Bottaro DP, Rubin JS, Faletto DL et al.: Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product. Science 1991;251:802
11 Grano M, Galimi F, Zambonin G, et al.: Hepatocyte growth factor is a coupling factor for osteoclasts and osteoblasts in vitro. Proc Natl Acad Sci USA 1996;93:7644
12 Wolfe SA: Autogenous bone grafts versus alloplastic material in maxillofacial surgery. Clin Plast Surg 1982;9:539
13 Takada S, Takushuwa S, Nakamura K et al.: Effect of hepatocyte growth factor on tacrolimus-induced nephrotoxicity in spontaneously hypertensive rats. Transplant Int 1999;12:27
14 Rashid MA, Akita S, Razzaque MS et al.: Coadministration of basic fibroblast growth factor and sucrose octasulfate(sucral-fate) facilitates the rat dorsal flap survival and viability. Plast Reconstr Surg 1999;103:941
15 Takai K, Hara J, Matsumoto K et al.: Hepatocyte growth factor is consititutively produced by human bone marrow stromal cells and indirectly promotes hematopoiesis. Blood 1997;89:1560
16 Skrtic S. Ohlsson C: Cortisol decreases hepatocyte growth factor levels in human osteoblast-like cells. Calcif Tissue Int 2000;66:108
17 Yaskashiwa M, Nakayama S, Ohnuma K et al.: Simultaneous or delayed administration of hepatocyte growth factor (HGF) equally repress the fibrotic change in murine lung injury induced by bleomycric a morphogenic study. Am J Reap Crit Care Med 1997;158:1937
18 Morishita R, Nakamura S, Hayashi S et al.: Therapeutic angiogenesis induced by human recombinant hepatocyte growth factor in rabbit hind limb ischemia model as cytokine supplement therapy. Hypertension 1999;33:1379
19 Miszuno S, Matsumoto K, Kurosawa T et al.: Reciprocal balance of hepatocyte growth factor and transforming growth factor-$\beta$1 in renal fibrosis in mice. Kidney Int 2000;57:937.
20 Krebsbach PH, Mankani MH, Satomura K et al.: Repair of crainotomy defect using bone marrow stromal cells. Transplantation 1998;66:1272
21 Ueki T, Kanada Y, Tsutiu H et al.: Hepatocyte growth factor gene therapy of liver chirrhosis in rats. Nature Med 1999;5:226
22 Kopp JB: Hepatocyte growth factor. mesenchymal signal for epithelial homeostasis. Kidney Int 1998;54:1392
23 Van Bolle E, Wizanbichter B, Chon D et al.: Potential angiogenic effect of scatter factor/hepatocyte growth factor via induction of vascular endothelial growth factor. the case for paracrine amplification of angiogenesis. Circulation 1998;97:381
24 Matsuda Y, Matsumoto K, Ichida T et al.: Hepatocyte growth factor suppresses the onset of liver cirrhosis and abrogates lethal hepatic dysfunction in rats. J Biochem 1995;118:643
25 Blanquaert F, Delany AM, Canalis E: Fibroblast growth factor-2 induces hepatocyte growth factor/scatter factor expression in osteoblasts. Endocrinology 1999;140:1069
26 Weimar IS, Miranda N, Muller EJ et al.: Hepatocyte growth factor/scatter factor(HGF/SF) is produced by human bone marrow stromal cells and promotes proliferation, adhesion and survival of human hematopoietic progenitor cells(CD34+). Exp Hematol 1998;26:885
27 Roos F, Ryan AM, Chamow SM et al.: Induction of liver growth in normal mice by infusion of hepatocyte growth factor/scatter factor. Am J Physiol 1995;268:G380
28 Thaller SR, Hoyt J, Borjeson K, et al.: Reconstruction of calvarial defects with anorganic bovine bone mineral(Bio-oss) in a rabbit model. J Craniofac Surg 1993;4:79
29 Busolino F, Di Renzo MF, Ziche M et al.: Hepatocyte growth factor is a potent angiogenic factor which stimulates endothelial cell motility and growth. J Cell Biol 1992;119:629
30 Birchmeier C, Gharardi E: Developmental roles of HGF/SF and its receptor, c-Met tyrosine kinase. Trends Cell Biol 1998;8:404
31 Minuno S, Kurosawa T, matsumoto K et al.: Hepatocyte growth factor prevents renal fibrosis and dysfunction in a mouse model of chronic renal disease. J Clin Invest 1998;113:1827
32 Wakitani S, Imoto K, Kimura T et al.: Hepatocyte growth factor facilitates cartilage repair. Full thickness articular cartilage defect studied in rabbit knees. Acta Orthop Scand 1997;68:474
33 Giovanni Z, Claudia C, Giobanni G et al.: Hydroxyapatite coated with hepatocyte growth factor(HGF) stimulates human osteoblasts in vitro. J bone Joint surg[Br] 2000;82-B:457
34 Takebayashi T, Iwamoto M, Jikko A et al.: Hepatocyte growth factor/scatter factor modulates cell motility, proliferation, and proteoglycan synthesis of chondrocytes. J Cell Biol 1995;129:1411
35 Tahara M, Matsumoto K, Nukiwa T et al.: Hepatocyte growth factor leads to recovery from alcohol-induced fatty liver in rats. J Clin Invest 1999;103:313
36 Schmassmann A, Stattler C, Pouleom R et al.: Roles of hepatocyte growth factor and receptor c-Met during gastric ulcer healing in rats. Gastroenterology 1997;113:1858