• Title/Summary/Keyword: Testing protocols

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Single Cell Gel Electrophoresis (comet assay) to Detect DNA Damage and Apoptosis in Cell Level (DNA damage와 Apoptosis를 정량화하는 단세포전기영동법)

  • 류재천;김현주;서영록;김경란
    • Environmental Mutagens and Carcinogens
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    • v.17 no.2
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    • pp.71-77
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    • 1997
  • The single cell gel electrophoressis(SCGE) assay, also known as the comet assay, is a rapid, simple, visual and sensitive technique for measuring and analysing DNA breakage in mammalian cells. The SCGE or comet assay is a promising test for the detection of DNA damage and repair in individnal cells. It has widespread potential applications in DNA damage and repair studies, genotoxicity testing and biomonitoring. In this microgel electrophoresis technique, cells are embedded in agarose gel on microscope slides, iysed and electrophoresed under alkaline conditions. Cells with increased DNA damage display increased migration of DNA from the nucleus towards the anode. The length of DNA migration indicates the amount of DNA breakage in the cell. The comet assay is also capable of identifying apoptotic cells which contain highly fragmented DNA. Here we review the development of the SCGE assay, existing protocols for the detection and analysis of comets, the relevant underlying principles determining the behaviour of DNA and the potential applications of the technique.

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A Study on the Start-up and Shut-down Characteristics for PEMFC System (고분자 연료전지시스템의 기동 및 정지특성에 관한 연구)

  • Lee, Jung-Woon;Seo, Won-Seok;Kim, Young-Gyu
    • 한국신재생에너지학회:학술대회논문집
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    • 2008.05a
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    • pp.29-32
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    • 2008
  • Testing was conducted to determine the performance of a residential fuel cell system when subjected to DSS and WSS operation, especially for start-up and shut-down characteristics. In terms of start-up time, it took about 70min to start output power generation and stably to reach 1kW at cold start. Measurement of the characteristics of heat and power generation were carried out at start-up and shut-down time. Fuel gas is used for heating both reformer and stack from start-up to the beginning of power generation. In terms of start-up and shut-down characteristics, it was important to control the reformer temperature precisely. The average output water temperature during the rated output operation(960W) was $63.2^{\circ}C$ constantly. The results of the investigation are being used to develop a new test protocols for residential fuel cell system.

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A Review of Some Representative Techniques for Controlling the Indoor Volatile Organic Compounds

  • Kabir, Ehsanul;Kim, Ki-Hyun
    • Asian Journal of Atmospheric Environment
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    • v.6 no.3
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    • pp.137-146
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    • 2012
  • Poor indoor air quality is now worldwide concern due to its adverse impacts on our health and environment. Moreover, these impacts carry a significant burden to the economy. Various technical approaches (e.g., biological, activated carbon fiber (ACF), photocatlytic oxidation (PCO), etc.) have gained popularity in controlling indoor volatile organic compounds (VOCs). This is because removing indoor VOC sources or increasing ventilation rates is often not feasible or economical. This review provides an overview of the various air purification technologies used widely to improve indoor air quality. Although most of these technologies are very useful to remove indoor VOCs, there is no single fully satisfactory method due to their diversity and presence at the low concentration. To achieve technical innovations and the development of specific testing protocols, one should possess a better knowledge on the mechanisms of substrate uptake at VOC concentrations.

Efficient Implementation of the MQTT Protocol for Embedded Systems

  • Deschambault, Olivier;Gherbi, Abdelouahed;Legare, Christian
    • Journal of Information Processing Systems
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    • v.13 no.1
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    • pp.26-39
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    • 2017
  • The need for embedded devices to be able to exchange information with each other and with data centers is essential for the advent of the Internet of Things (IoT). Several existing communication protocols are designed for small devices including the message-queue telemetry transport (MQTT) protocol or the constrained application protocol (CoAP). However, most of the existing implementations are convenient for computers or smart phones but do not consider the strict constraints and limitations with regard resource usage, portability and configuration. In this paper, we report on an industrial research and development project which focuses on the design, implementation, testing and deployment of a MQTT module. The goal of this project is to develop this module for platforms having minimal RAM, flash code memory and processing power. This software module should be fully compliant with the MQTT protocol specification, portable, and inter-operable with other software stacks. In this paper, we present our approach based on abstraction layers to the design of the MQTT module and we discuss the compliance of the implementation with the requirements set including the MISRA static analysis requirements.

Develpoment of Automatic Meter Reading Program for Electronic Power Meter (전자식 전력량계용 한전 프로토콜 및 운영 프로그램 개발)

  • Yu, In-H.;Jang, Mun-J.;Hyun, Duk-H.
    • Proceedings of the KIEE Conference
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    • 2001.07d
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    • pp.2219-2221
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    • 2001
  • A communication protocol for automatic meter reading is designed for electronic power meter. Communicational functions and data structures are standardized and data reading software is developed according to the developed protocol. The communication protocol is designed with reference specifications, IEC 62056-31 and Distributed Network Protocol(DNP). For design, communicational functions and specifications of the industrial meters were analyzed for the purpose of developing a new one for KEPCO meters. The protocol is designed with 3 layer model, physical layers, data link layer and application layer. A documentation was completed with data gathering, data classification, authentication, error detection. An emulator system was made for testing the protocol. A meter reading software was developed using the protocol. Many different protocols and meter reading softwares are integrated into one with the developed protocol and software as KEPCO's standard.

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A Distributed Control Architecture for Advanced Testing In Realtime

  • Thoen Bradford K.;Laplace Patrick N.
    • Proceedings of the Earthquake Engineering Society of Korea Conference
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    • 2006.03a
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    • pp.563-570
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    • 2006
  • Distributed control architecture is based on sharing control and data between multiple nodes on a network Communication and task sharing can be distributed between multiple control computers. Although many communication protocols exist, such as TCP/IP and UDP, they do not have the determinism that realtime control demands. Fiber-optic reflective shared memory creates the opportunity for realtime distributed control. This architecture allows control and computational tasks to be divided between multiple systems and operate in a deterministic realtime environment. One such shared memory architecture is based on Curtiss-Wright ScramNET family of fiber-optic reflective memory. MTS has built seismic and structural control software and hardware capable of utilizing ScramNET shared memory, opening up infinite possibilities in research and new capabilities in Hybrid and Model-In-The-Loop control.

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Toxicity of nanoparticles_ challenges and opportunities

  • Ramanathan, Amall
    • Applied Microscopy
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    • v.49
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    • pp.2.1-2.11
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    • 2019
  • Nanomaterials (NMs) find widespread use in different industries that range from agriculture, food, medicine, pharmaceuticals, and electronics to cosmetics. It is the exceptional properties of these materials at the nanoscale, which make them successful as growth promoters, drug carriers, catalysts, filters and fillers, but a price must be paid via the potential toxity of these materials. The harmful effects of nanoparticles (NPs) to environment, human and animal health needs to be investigated and critically examined, to find appropriate solutions and lower the risks involved in the manufacture and use of these exotic materials. The vast number and complex interaction of NM/NPs with different biological systems implies that there is no universal toxicity mechanism or assessment method. The various challenges need to be overcome and a number of research studies have been conducted during the past decade on different NMs to explore the possible mechanisms of uptake, concentrations/dosage and toxicity levels. This review article examines critically the recent reports in this field to summarize and present opportunities for safer design using case studies from published literature.

Neuromodulation for Insomnia Management (불면증 치료법으로서의 뉴로모듈레이션)

  • Yoon, Ho-Kyoung
    • Sleep Medicine and Psychophysiology
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    • v.28 no.1
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    • pp.2-5
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    • 2021
  • Hyperarousal or increased brain excitability is thought to play a key role in the pathophysiology of insomnia. Neuromodulation techniques are emergent complementary therapies for insomnia and can improve sleep by modulating cortical excitability. A growing body of literature support the idea that neuromodulation can be effective in improving sleep or treating insomnia. Recent evidence has revealed that neuromodulation methods can improve objective and subjective sleep measures in individuals with insomnia, although effects vary according to protocol. Different mechanisms of action might explain the relative efficacy of neuromodulation techniques on sleep outcomes. Further research testing different stimulation parameters, replicating existing protocols, and adding standardized sleep-related outcomes could provide further evidence on the clinical utility of neuromodulation techniques.

Deriving and Applying on SW Quality Characteristics of AIS based on ISO/IEC 25023 (ISO/IEC 25023 기반 AIS 품질특성별 SW 평가항목 도출 및 적용 연구)

  • Kim, Min-Woo;Park, Ji-Min
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.25 no.12
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    • pp.1956-1959
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    • 2021
  • AIS(Automatic Identification System) provides navigational information including identification, position, a ship's course and status to ground and other vessels. To obtain AIS Marine Equipment Approval Service, various requirements are required and meet the requirements International Standards. However, most of the requirements are to identify essential functions, response time, hardware requirements, and communication protocols of AIS. The requirements for the quality of SW are not sufficient or detailed, and the weight is relatively low. As role of SW grows and types become more diverse, AIS SW quality inspection is essential. In this paper, We apply eight-quality characteristics of ISO/IEC 25023 standard to improve SW coverage quality of AIS. Suggest additional AIS SW requirements based on the eight quality characteristics of ISO/IEC 25023 standard.

Implementation of VANET Simulator using Matlab (Matlab을 이용한 VANET 시뮬레이터 구현)

  • Nam, Jae-hyun
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.20 no.6
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    • pp.1171-1176
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    • 2016
  • VANET are a special kind of MANET adapted to the communications between vehicles. In this work, we propose the models used for representing the communication among vehicles, vehicles mobility features, and VANET simulator using realistic vehicular mobility models. VANET requires that a traffic and network simulator should be used together to perform this test. But, simulator tool has been preferred over traffic simulation because it simple, easy and cheap. Goal of this paper is to create a simulation of Vehicular Ad-Hoc network for urban scenario which can be used for testing purposes. Such environment can be used while designing better MAC protocols in VANETs. We are evaluating the performance of two routing protocols namely AODV and DSDV. The comparison was based on the throughout, packet loss and end-to-end delay. We found that DSDV has less throughput performance and high routing overhead than AODV. On average end-to-end delay and packet loss, AODV performs better than DSDV.