• Journal of Korean Society of Coastal and Ocean Engineers
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• v.25 no.1
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• pp.11-19
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• 2013

As the temperature data show a distribution pattern with a number of peaks, assumption of normal distribution will result in a serious bias in the analysis. In this study, the Gaussian Mixture Distribution (GMD), a type of bimodal distribution, is presumed as a frequency distribution for the water temperature, in order to estimate the optimal parameter and to carry out the relation analysis between the optimal parameter and the basic statistical information such as mean and variance on the data. By the way, an estimation formulae to compute the frequency distribution of the data is developed by computing the parameters of GMD (i.e. ${\alpha}_1$, ${\mu}_1$, ${\sigma}_1$, ${\alpha}_2$, ${\mu}_2$, ${\sigma}_2$) by means of the major characteristic values, such as mean, standard deviation and skewness of the data. The formulae shows an excellent coincidence with the result from the observation data, in the RMS limit accuracy of 5%.

• Journal of Environmental Impact Assessment
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• v.26 no.5
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• pp.331-343
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• 2017

The important changes in water environment management in Korea can be summarized as the enactment of Act on the Integrated Control of Pollutant-Discharging Facilities. Therefore water quality impact assessment should be reexamined and be revised. This study examines the present water quality impact assessment items (permissible discharge limits, standards for effluent water quality including Total Pollutant Load Management System) and considers the land use regulation for water quality conservation and NVZs(Nitrate Vulnerable Zones of EU and England). It also considers lately adopted standards(maximum discharge standards, permissible discharge standards, and marginal discharge standards etc) based on Act on the Integrated Control of Pollutant-Discharging Facilities and then compares Korean BAT and its counterpart control technology of U.S.A. And it also compares the items of water quality impact assessment with those of Integrated Control of Pollutant-Discharging Facilities, based on EIS reporting items. This study suggests five improvement measures for water quality impact assessment. First reflection of discharge impact analysis on impact prediction and assessment, second reflection of permissible discharge standards on agreed standards in the EIA procedure, third, reflection of diversified BAT on mitigation measures in the EIA procedure, forth introduction of land use regulation such as NVZs, finally strengthening linkage between water quality items and land use items etc.

• Economic and Environmental Geology
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• v.27 no.1
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• pp.101-113
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• 1994

Geochemical investigations based on measurements of water parameters and sampling of stream sediments have been carried out, in the Okdongcheon stream and its tributaries in the Sangdong area of South Korea. There are two main problems occurring in the Okdongcheon stream: an acid mine drainage in the upper reaches and toxic trace metal contamination of the stream sediments mainly in the lower reaches. Acid mine water originating from coal mining was neutralized at the confluence of the Cheonpyongcheon stream whilst suspended solids due to flocculation of iron in water caused turbidity which was undesirable. Sediments in the Okdongcheon stream have been contaminated by mining activites. Iron was heavily concentrated in sediments in the upper Okdongcheon whilst toxic trace metals including Pb, Cu, Zn, Co, Cd, As and Bi were accumulated in sediments at stations draining metallic mining areas and near the tailings dam. There is now a requrement to neutralise the acid mine drainage and to use site-specific analysis of biological communities to ensure the conservation and preservation of aquatic organisms.

• Horticultural Science & Technology
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• v.31 no.3
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• pp.337-343
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• 2013

The aims of this study were to compare genetic distances among 14 Phalaenopsis varieties using simple sequence repeat (SSR) and sequence-related amplified polymorphism (SRAP) marker systems and to determine the discrimination using SSR. A total of 111 SSR primers and 30 SRAP combinations were initially screened. Twelve SSR primers and thirty SRAP combinations showed high polymorphism among the 14 Phalaenopsis varieties including domestic breeding varieties, conserved in National Institute of Horticultural & Herbal Science (NIHHS). The amplified DNA fragments were separated by denaturing acrylamide gels and detected by silver staining method. A total of 474 polymorphic bands, including 55 by SSRs and 419 by SRAPs, were identified and used for genetic diversity analysis. Polymorphic bands were scored for calculating a simple matching coefficient of genetic similarity and cluster analysis with multi-variate statistical package (MVSP) 3.1. Fourteen Phalaenopsis varieties were classified into three major groups at similarity coefficient value of 0.683 and 0.66 using SRAP and SSR, respectively. Also we could discriminate these domestic breeding Palaenopsis varieties using only SSR 20 and SSR 22. The results indicate that SSR analysis is effective for discrimination among Phalaenopsis varieties and SRAP is useful for genetic diversity when there is no sequence information. These studied SSR and SRAP markers will be useful tools for genotype identification, germplasm conservation and genetic relationship study in Phalaenopsis.

• Korean Journal of Poultry Science
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• v.36 no.4
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• pp.323-328
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• 2009

In order to determine the origin and genetic diversity among chicken breeds, mitochondrial (mt) DNA D-loop sequences have been widely used. In this study, 41 individuals from four breeds (Korean native chicken (Black and Brown) and two imported breeds, Rhode Island Red and Cornish) were used for identifying genetic relationships with other chicken breeds. We obtained ten haplotypes and the highest number of haplotype was represented by eight individuals each from haplotype 1 and haplotype 2. Neighbor-joining phylogenetic tree indicates that the black and brown Korean native chicken breeds were mixed in haplotype 2 and they were closely related with the red jungle fowl (Gallus gallus). We also investigated whether the D-loop hypervariable region in chicken mtDNA can be used for the breed identification marker. The results indicated that the combination of the SNPs in the D-loop region can be possibly used for the breed discriminating markers. The results obtained in this study can be used for designing proper breeding and conservation strategies for Korean native chicken, as well as development of breed identification markers.

• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.388-395
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• 2015

Somatic embryogenesis is as an excellent technology for potential use in plant mass production, germplasm conservation, or genetic engineering. We examined the effect of cold storage using 3 embryogenic callus lines with different levels of embryogenesis competence derived from immature zygotic embryo cultures of Kalopanax setemlobus. Somatic embryo induction, germination and plant conversion were evaluated after 1, 3 and 6 months storage at $4^{\circ}C$ in the dark. Most cold-stored embryogenic calli formed somatic embryos normally even after 6 months; however, the induction rate was gradually decreased by increasing the storage period. The most competent line tended to show a slight decline in somatic embryo induction rate, as compared with other lines after cold storage. In general, cold storage resulted in reduced somatic embryo germination and plant regeneration, although 93% somatic embryo germination and 91% plant conversion were achieved regardless of the storage period. Cold storage led to cell browning and degradation. Additionally, the cell structures were confirmed by the aceto-carmine and evans blue dye evaluation. Collectively, our results showed that embryogenic callus of K. septemlobus could be preserved at $4^{\circ}C$ without subculture for 6 months, and suggested the need for storage of relatively more competent embryogenic calli lines to support somatic embryo induction.

• Journal of Wetlands Research
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• v.13 no.3
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• pp.455-463
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• 2011

Conventional construction technologies have been continually applied without consideration of its impact to the environment. This resulted to various problems including the negative responses of local citizens that regarded some constructed facilities as aversive facilities causing environmental and hydraulic problems in the urban area, etc. To prevent these problems, therefore, alternative methods should be undertaken. A new approach termed "Low Impact Development (LID)" technology is currently adapted in developed countries around the world. This study aims to investigate the efficiency of the developed small constructed wetland (SCW) with horizontal subsurface flow as a LID technique applicable in urban areas. Two test-bed facilities were constructed and monitoring had been conducted between July 2010 and June 2011. Based on the findings, the removal efficiencies achieved for TSS, $COD_{Cr}$, TN, TP, Total Fe, Total Pb for the SCW-1 were 66, 53, 46, 55, 67 and 50%, respectively. On the other hand, the SCW-2 attained 82, 62, 51, 48, 74 and 42% efficiency for TSS, $COD_{Cr}$, TN, TP, Total Fe, Total Pb, respectively. The results indicated that the removal of particulate matter and heavy metals which are considered as main pollutants from stormwater runoff in urban areas was satisfactory in the system. Therefore, the test-beds proved to be appropriate for the treatment of pollutants in urban landuses such as road, parking lot, etc. The results of this study can contribute to the conservation of aquatic ecosystems and restoration of natural water cycle in the urban areas.

• Journal of the Mineralogical Society of Korea
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• v.28 no.2
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• pp.135-145
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• 2015

In order to predict the deterioration of stone monument due to acid fog, an artificial fog test using pH4.0 and pH5.6 was applied to the Gyeongju Namsan granite, decite and marble. After the test had weathered Gyeongju Namsan granite a larger weight reduction due to acid fog than fresh one. Decite has shown the most significant changes among the tested rocks with about 0.005 % of weight reduction. Decite and weathered granite will have considerable weight reduction due to acid rain than the acid fog, whereas the marble was expected to show a weight reduction regardless of the phase of water. The porosity and water absorption rate of weathered granite had significantly increased. This result means that the weathered rock is predicted to be more susceptible to acid fog than the fresh rock. The absorption rate of the marble after the test had shown approximately 50 % increase. The color of the samples had slightly changed towards yellow, such tendency was greater shown in weathered rocks. The marble reacted with acid fog had an increased whiteness. A large amount of cation in the samples is caused mainly by the dissociation of minerals through the reaction with acid fog.

• International Journal of Highway Engineering
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• v.12 no.1
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• pp.79-86
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• 2010

Cold in-place recycling (CIR) using emulsified asphalt or foamed asphalt has become a more common practice in rehabilitating the existing asphalt pavement due to its cost effectiveness and the conservation of paving materials. As CIR continues to evolve, the engineered emulsified asphalt was developed to improve the field performances such as coating, raveling, retained stability value and curing time. The main objective of this research is to compare the laboratory responses of the engineered emulsified asphalt (CIR-EE) mixtures against the foamed asphalt (CIR-foam) mixtures using the reclaimed asphalt pavement (RAP)materials collected from the CIR project on U.S. 20 Highway in Iowa. Based on the visual observation of laboratory specimens, the engineered emulsified asphalt coated the RAP materials better than the foamed asphalt because the foamed asphalt is to create a mastic mixture structure rather than coating RAP materials. Given the same compaction effort, CIR-EE specimens exhibited lesser density than CIR-foam specimens. Both Marshall stability and indirect tensile strength of CIR-EE specimens were about same as those of CIR-foam specimens. However, Marshall stability and indirect tensile strength of the vacuum-saturated wet specimens of CIR-EE mixtures were higher than those of CIR-foam mixtures. After four hours of curing in the room temperature, the CIR-EE specimens showed less raveling than the CIR-foam specimens. On the basis of test results, it can be concluded that the CIR-EE mixtures is less susceptible to moisture and more raveling resistant than CIR-foam mixtures.

• Journal of Plant Biotechnology
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• v.47 no.2
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• pp.157-163
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• 2020

Calmodulin (CaM) mediates cellular Ca²⁺ signals in the defense responses of plants. We previously reported that GmCaM-4 and 5 are involved in salicylic acid-independent activation of disease resistance responses in soybean (Glycine max). Here, we generated a GmCaM-4 cDNA construct under the control of the cauliflower mosaic virus (CaMV) 35S promoter and transformed this construct into potato (Solanum tuberosum L.). The constitutive over-expression of GmCaM-4 in potato induced high-level expression of pathogenesis-related (PR) genes, such as PR-2, PR-3, PR-5, phenylalanine ammonia-lyase (PAL), and proteinase inhibitorII (pinII). In addition, the transgenic potato plants exhibited enhanced resistance against a bacterial pathogen, Erwinia carotovora ssp. Carotovora (ECC), that causes soft rot disease and showed spontaneous lesion phenotypes on their leaves. These results strongly suggest that a CaM protein in soybean, GmCaM-4, plays an important role in the response of potato plants to pathogen defense signaling.