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http://dx.doi.org/10.5010/JPB.2020.47.2.157

Identification of disease resistance to soft rot in transgenic potato plants that overexpress the soybean calmodulin-4 gene (GmCaM-4)  

Park, Hyeong Cheol (Team of Vulnerable Ecological Research, Division of Climate and Ecology, Bureau of Conservation & Assessment Research, National Institute of Ecology)
Chun, Hyun Jin (Division of Applied Life Science (BK21 Plus Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University)
Kim, Min Chul (Division of Applied Life Science (BK21 Plus Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University)
Lee, Sin Woo (Department of Agronomy & Medicinal Plant Resources, Gyeongnam National University of Science & Technology)
Chung, Woo Sik (Division of Applied Life Science (BK21 Plus Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University)
Publication Information
Journal of Plant Biotechnology / v.47, no.2, 2020 , pp. 157-163 More about this Journal
Abstract
Calmodulin (CaM) mediates cellular Ca2+ signals in the defense responses of plants. We previously reported that GmCaM-4 and 5 are involved in salicylic acid-independent activation of disease resistance responses in soybean (Glycine max). Here, we generated a GmCaM-4 cDNA construct under the control of the cauliflower mosaic virus (CaMV) 35S promoter and transformed this construct into potato (Solanum tuberosum L.). The constitutive over-expression of GmCaM-4 in potato induced high-level expression of pathogenesis-related (PR) genes, such as PR-2, PR-3, PR-5, phenylalanine ammonia-lyase (PAL), and proteinase inhibitorII (pinII). In addition, the transgenic potato plants exhibited enhanced resistance against a bacterial pathogen, Erwinia carotovora ssp. Carotovora (ECC), that causes soft rot disease and showed spontaneous lesion phenotypes on their leaves. These results strongly suggest that a CaM protein in soybean, GmCaM-4, plays an important role in the response of potato plants to pathogen defense signaling.
Keywords
Calmodulin; Disease resistance; Erwinia (soft rot); Pathogen-related gene; Potato;
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