Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
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Analysis of Genetic Diversity and Identification of Domestic Bred Phalaenopsis Varieties Using SRAP and SSR Markers

SRAP과 SSR 마커를 이용한 국내 육성 팔레놉시스 품종의 유전적 다양성 분석과 품종판별

  • Park, Pue Hee (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Park, Yong-Jin (Department of Plant Resources, College of Industrial Science, Kongju National University) ;
  • Kim, Mi Seon (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Lee, Young Ran (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Park, Pil Man (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Lee, Dong Soo (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Yae, Byeong Woo (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
  • 박부희 (국립원예특작과학원 화훼과) ;
  • 박용진 (공주대학교 산업과학대학 식물자원학과) ;
  • 김미선 (국립원예특작과학원 화훼과) ;
  • 이영란 (국립원예특작과학원 화훼과) ;
  • 박필만 (국립원예특작과학원 화훼과) ;
  • 이동수 (국립원예특작과학원 화훼과) ;
  • 예병우 (국립원예특작과학원 화훼과)
  • Received : 2012.11.06
  • Accepted : 2013.02.08
  • Published : 2013.06.30
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Abstract

The aims of this study were to compare genetic distances among 14 Phalaenopsis varieties using simple sequence repeat (SSR) and sequence-related amplified polymorphism (SRAP) marker systems and to determine the discrimination using SSR. A total of 111 SSR primers and 30 SRAP combinations were initially screened. Twelve SSR primers and thirty SRAP combinations showed high polymorphism among the 14 Phalaenopsis varieties including domestic breeding varieties, conserved in National Institute of Horticultural & Herbal Science (NIHHS). The amplified DNA fragments were separated by denaturing acrylamide gels and detected by silver staining method. A total of 474 polymorphic bands, including 55 by SSRs and 419 by SRAPs, were identified and used for genetic diversity analysis. Polymorphic bands were scored for calculating a simple matching coefficient of genetic similarity and cluster analysis with multi-variate statistical package (MVSP) 3.1. Fourteen Phalaenopsis varieties were classified into three major groups at similarity coefficient value of 0.683 and 0.66 using SRAP and SSR, respectively. Also we could discriminate these domestic breeding Palaenopsis varieties using only SSR 20 and SSR 22. The results indicate that SSR analysis is effective for discrimination among Phalaenopsis varieties and SRAP is useful for genetic diversity when there is no sequence information. These studied SSR and SRAP markers will be useful tools for genotype identification, germplasm conservation and genetic relationship study in Phalaenopsis.

본 연구의 목적은 SSR과 SRAP 마커 시스템을 이용하여 팔레놉시스 14품종 간 유전적 거리를 비교하고, SSR 마커를 이용하여 품종 간 구분을 하기 위한 것이다. 전체적으로 111개의 SSR 프라이머와 30조합의 SRAP primer를 먼저 스크리닝하였다. 국립원예특작과학원에서 보존중인 국내 육성품종을 포함한 14품종의 팔레놉시스에서 12개의 SSR 프라이머와 30조합의 SRAP 프라이머에서 높은 다형성을 보였다. 증폭된 DNA 단편들은 acrylamide gel에서 분리시킨 후 silver staining 방법으로 검출하였다. SSR 마커 55개와 SRAP 419개로, 총 474개의 마커를 획득하였으며 이를 유전적 다양성 분석에 사용하였다. 다형성 밴드들은 MVSP 3.1프로그램을 이용하여 유전적 유사도와 UPGMA clustering 분석을 위해 scoring 되었다. 14 팔레놉시스 품종은 SRAP과 SSR 분석을 통해 각각 0.683과 0.66의 유사도 지수에서 3그룹으로 분류되었다. 또한 SSR 20번과 22번만으로도 이들 육성 품종을 구분할 수 있었다. 이 결과는, SSR 분석은 팔레놉시스 품종간 구분에 효과적이고 SRAP은 염기서열의 정보가 없을 때 유전적 다양성 분석에 유용하다는 것을 보여준다. 이번 연구된 SSR과 SRAP 마커들은 팔레놉시스의 유전자형 판별, 유전자원 보존, 유전적 근연관계를 분석하는데 유용한 기술이 될 것이다.

Keywords

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