• Research in Mathematical Education
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• v.14 no.1
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• pp.11-18
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• 2010

The basic idea of cooperative learning focuses on team reward, equal opportunities for success, cooperation within team and competition among teams, and emphasizes share of sense of achievement through joint efforts so as to realize specific learning objectives. The main strategies of engineering mathematics teaching based on cooperative learning are to establish favorable team and design reasonable team activity plan. During the period of team establishment, attention shall be given to team structure including such elements as team status, role, norm and authority. Team activity plan includes team activity series and team activity task. Team activity task shall be designed to be a chain of questions following a certain principle.

• Journal of Engineering Education Research
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• v.20 no.2
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• pp.39-49
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• 2017

This research aims to explore the relationships between 'teamwork skills' (often called team activity competence) and 'thinking styles' of engineering students in Korea, and to provide an explanation for the application of team-based environment as well as for the increase of teamwork skills. Teams and team activity are pervasive in today's organization and there has been relatively much research on teamwork skills and its related factors. However, to date, little attention has been paid to the teamwork skills, essential factor in team-based environment and its relationships with thinking styles. This study was conducted with 383 engineering students at P University, and students' teamwork skills as well as thinking styles have been measured before and after team-based learning class (hereafter TBL). Our findings show that firstly, there was a significant increase of teamwork skills between before and after TBL class. Second, team activity competence was found to have a higher correlation with most of creativity generating styles (i.e. legislative, judicial, hierarchical and global styles). Third, hierarchical style was found to influence team activity more than other components, and also legislative, external, global and judicial styles contributed to team-based activity. These findings are expected to provide an explanation for the application of thinking styles in team-based environment and will be useful for the improvement of related courses in engineering school.

• Journal of Engineering Education Research
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• v.18 no.4
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• pp.26-33
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• 2015

The purpose of this study is to microscopically analyze the problem solving activities in creative robotics making and programming activity in order to investigate the characteristics of team interaction in accordance with MBTI disposition of team members in the creative robotics making activity in which the engineering education has a lot interest in the recent years. For the prosecution of this study, teams composed of 2 students targeting 30 students who were at the related department of engineering education in the college of eduction, A university located in Daejeon were organized. In addition, individual propensities of team members, in other words, team dynamic structure and team interaction were analyzed through microscopical analysis of the problem solving activities in creative robotics making and programming activity. The results of this study showed that there was a static correlation between the organization of MBTI Heterogeneity team and the interaction of team members, and also there was a positive interaction of team members in organizing MBTI Homogeneity team in area of Thought (T) - Feeling (F). In addition, mutual complement to solve the problems was carried out and the problem-solving capability was shown in the course of the communication along with the organization of MBTI team of extreme heterogeneity.

• Journal of Microbiology
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• v.44 no.6
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• pp.622-631
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• 2006

In this study we purified a fibrinolytic enzyme from Cordyceps militaris using a combination of ion-exchange chromatography on a DEAE Sephadex A-50 column, gel filtration chromatography on a Sephadex G-75 column, and FPLC on a HiLoad 16/60 Superdex 75 column. This purification protocol resulted in a 191.8-fold purification of the enzyme and a final yield of 12.9 %. The molecular mass of the purified enzyme was estimated to be 52 kDa by SDS-PAGE, fibrin-zymography, and gel filtration chromatography. The first 19 amino acid residues of the N-terminal sequence were ALTTQSNV THGLATISLRQ, which is similar to the subtilisin-like serine protease PR1J from Metarhizium anisopliae var. anisopliase. This enzyme is a neutral protease with an optimal reaction pH and temperature of 7.4 and $37^{\circ}C$, respectively. Results for the fibrinolysis pattern showed that the enzyme rapidly hydrolyzed the fibrin $\alpha$-chain followed by the $\gamma$-$\gamma$ chains. It also hydrolyzed the $\beta$-chain, but more slowly. The A$\alpha$, B$\beta$, and $\gamma$ chains of fibrinogen were also cleaved very rapidly. We found that enzyme activity was inhibited by $Cu^{2+}$ and $Co^{2+}$, but enhanced by the additions of $Ca^{2+}$ and $Mg^{2+}$ ions. Furthermore, fibrinolytic enzyme activity was potently inhibited by PMSF and APMSF. This enzyme exhibited a high specificity for the chymotrypsin substrate S-2586 indicating it's a chymotrypsin-like serine protease. The data we present suggest that the fibrinolytic enzyme derived from the edible and medicinal mushroom Cordyceps militaris has fibrin binding activity, which allows for the local activation of the fibrin degradation pathway.

• Knowledge Management Research
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• v.6 no.2
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• pp.91-108
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• 2005

In recent years, organizations have chosen a team-organization as a standard activity level and it leads a significant change in process of knowledge producing. Nevertheless team-organization for building up effective performance and a good communication in organization has influenced knowledge management performance, the empirical analysis and survey have not proved yet. This study, therefore, makes an analysis that the change of organization base on team activity influences knowledge management performance. This study, based on 312 data by using LISREL as a model for studying, provides a result that a team-based activity positively influences knowledge management efficiency.

• Journal of Engineering Education Research
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• v.19 no.1
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• pp.11-20
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• 2016

Team activities in engineering curriculum play the major role in nourishing students with problem-solving and team-working capabilities. Educators endeavor to guide students to achieve success experiences through team activities. The sense of success depends on how the students think and feel about team activities. In this study, we investigated the students thoughts on team activity in various aspects. We provided freshmen and juniors with a set of questionnaire and gathered answers, from which we tried to elicit implication that we may apply for coaching their team activities. The analysis result shows that the perception level of freshmen on team activities is less mature in a sense that the freshmen's view is more neutral than that of juniors. Regardless of the grade, they show that they put more emphasis on the team atmosphere than on the team performance. Especially the freshmen are more sensitive to the relationship between the team members than the juniors. The juniors concern more about whether they prepare well enough for the team activities or not. Both of the grades prefer self-regulation in team formation and think the leadership is essential in performing team activities. However, they both hesitate to take a role as a leader. We expect the research result will provide novel ideas for coaching students to obtain valuable experiences on team activities.

• The Korea Journal of Herbology
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• v.26 no.1
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• pp.13-19
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• 2011

Objective : This study was to compare antioxidant activity and HPLC pattern analysis from 4 species of changpo(ch$\={a}$ngp$\'{u}$). Methods : To compare the antioxidant activity and HPLC pattern analysis from the 4 species of changpo, we performed the in vitro anti-oxidative activity assays and HPLC analysis from 70% ethanol extracts of Acorus gramineus Sol. (=AG), A. tatarinowii Schott (=AT), A. calamus L. (=AC) and Anemone altaica Fisch. ex C.A.Mey (=AA) taken in the herbal medicine market of Korea. Results : AG has the most effective anti-oxidative activity among 4 species of changpo. As the HPLC pattern analysis, AT was detected the unknown peak at retention time 14.9 min whereas AG was not showed any peak at the same retention time. These results suggest that AG could be used rather than AT when it need to be prescribed as anti-oxidative medicine. Conclusions : This result can be used as the basic data contributing to the stability of AG according to an appropriate clinical application.

• Journal of Microbiology and Biotechnology
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• v.17 no.8
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• pp.1271-1283
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• 2007

A fibrinolytic protease (PoFE) was purified from the cultured mycelia of the edible oyster mushroom Pleurotus ostreatus, using a combination of various chromatographies. The purification protocol resulted in an 876-fold purification of the enzyme, with a final yield of 6.5%. The apparent molecular mass of the purified enzyme was estimated to be 32 kDa by SDS-PAGE, fibrin-zymography, and size exclusion using FPLC. The optimal reaction pH value and temperature were pH 6.5 and $35^{\circ}C$, respectively. PoFE effectively hydrolyzed fibrinogen, preferentially digesting the $A{\alpha}$-chain and the $B{\beta}$-chain over the ${\gamma}$-chain. Enzyme activity was enhanced by the addition of $Ca^{2+},\;Zn^{2+},\;and\;Mg^{2+}$ ions. Furthermore, PoFE activity was potently inhibited by EDTA, and it was found to exhibit a higher specificity for the chromogenic substrate S-2586 for chymotrypsin, indicating that the enzyme is a chymotrypsin-like metalloprotease. The first 19 amino acid residues of the N-terminal sequence were ALRKGGAAALNIYSVGFTS, which is extremely similar to the metalloprotease purified from the fruiting body of P. ostreatus. In addition, we cloned the PoFE protein, encoding gene, and its nucleotide sequence was determined. The cDNA of cloned PoFE is 867 nucleotides long and consists of an open reading frame encoding 288 amino acid residues. Its cDNA showed a high degree of homology with PoMEP from P. ostreatus fruiting body. The mycelia of P. ostreatus may thus represent a potential source of new therapeutic agents to treat thrombosis.

• Journal of Fisheries and Marine Sciences Education
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• v.27 no.1
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• pp.108-114
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• 2015

The purpose of this study is to find the relationships among communication competency, social network centrality(trust centrality and knowledge sharing centrality), discussion performance, and online boarding activity in the team based learning situation. For investigating this topic, 44 students are participated in the classes of educational technology. In order to find out the relationships among communication competency, social network centrality, discussion performance, and online boarding activity, compared t-test and path analysis are used. Followings are the results of the research: (a) Communication competency is improved significantly after team based learning. (b) Trust centrality effects significantly on the knowledge sharing centrality. (c) Knowledge sharing effects significantly on discussion performance. (d) Trust centrality effects on the online boarding activity in the team based learning.

• Journal of Engineering Education Research
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• v.17 no.5
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• pp.23-27
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• 2014

Student independent project activity is beneficial for enhancing many kinds of abilities, e.g., team working, collaboration, communication, presentation, creativity, leadership, and so on. More than ten project teams actively perform every year in the Center for Innovation and Creativity Development of The University of Tokushima. The project management workshop was planned in order to make project activity successful. In this workshop, the team members studied the importance of holding a common objective among them and of making a clear plan for achieving the activity. They practiced brainstorming method and KJ method and experienced a PDCA cycle. The team activity will be fruitful on the basis of the project management method.