• Journal of Microbiology and Biotechnology
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• 제17권11호
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• pp.1848-1855
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• 2007

Antibacterial activity of essential oils (Tea tree, Chamomile, Eucalyptus) on Staphylococcus aureus growth was evaluated as well as the essential oil-loaded alginate beads. The binding interactions between the cell and the essential oils were measured using an optical biosensor. The antibacterial activity of the essential oils to the cell was evaluated with their binding interaction and affinity. The antibacterial activity appeared in the order of Tea Tree>Chamomile>Eucalyptus, in comparison of the inhibition effects of the cell growth to the essential oils. The association rate constant and affinity of the cell binding on Tea Tree essential oil were $5.0{\times}10^{-13}\;ml/(CFU{\cdot}s)$ and $5.0{\times}10^5\;ml/CFU$, respectively. The affinity of the cell binding on Tea Tree was about twice higher than those on the other essential oils. It might be possible that an effective antibacterial activity of Tea Tree essential oil was derived from its strong adhesive ability to the cell, more so than those of the other essential oils.

• Korean Chemical Engineering Research
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• 제56권2호
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• pp.240-244
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• 2018

There is growing concern about cross infection among the patients to patients, patients to staffs, and tools to patients in healthcare facilities, especially in dentistry. In this study, the most widely used dental impression materials were prepared and the synergy effect of Chlorhexidine and essential oil on antimicrobial activity was examined in the impression materials. Chlorhexidine concentration of 0.1 wt% and 0.5 wt% showed no antimicrobial activity on Escherichia coli (E. coli) and Candida albicans. At 1.0 wt% Chlorhexidine, 0% of E. coli and 34.7% of Candida albicans were survived. Bergamot (Essential oil) concentration of 0.5 wt% and 1.0 wt% showed no antimicrobial activity on E. coli. At 2.0 wt% Bergamot oil, 71.9% of E. coli were survived. Tea tree oil (Essential oil) of 0.5 wt% showed no antimicrobial activity on E. coli. At 1.0 wt% Tea tree oil, 11.2% of E. coli was survived. At 2.0 wt% Tea tree oil, no E. coli was survived. However, no E. coli was survived at the concentration of 0.8 wt% Bergamot with 0.3 wt% Chlorhexidine. At the concentration of 0.8 wt% Tea Tree oil with 0.3 wt% Chlorhexidine, 1.3% of E. coli were survived. The experimental results showed that the synergy effects between Chlorhexidine and essential oils on antimicrobial activity were prominent.

• 한국융합학회논문지
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• 제9권7호
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• pp.149-154
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• 2018

본 논문은 Lavender, Tea tree, Eucalyptus, Lemongrass 4종의 천연에센셜오일의 Streptpccous mutans 에 의한 치석형성억제 효과에 대하여 연구해 보고자 하였다. Streptpccous mutans 에 의한 치석형성 억제 활성 측정결과인 Lavender 에센셜 오일의 MAC (Minium anti-adhesive concentration, 최소부착저지 농도)가 1.0 % 이고 Tea tree 에센셜 오일, Eucaliptus 에센셜 오일, lemongrass 에센셜 오일의 MAC는 0.5 % 로 나타났다. 에센셜오일의 농도를 달리하여 Streptpccous mutans와 혼합한 배양액을 항온조에서 배양한 후 용액의 pH를 측정하여 최소부착 저지농도 이하에서 산의 생성으로 pH가 낮아진 것을 확인할 수 있었고, 이 결과는 에센셜 오일이 Streptpccous mutans 에 의한 산의 생성을 억제하는 효과가 있다는 것이다. 이러한 결과들로부터 천연에센셜오일 중 특히 Tea tree, Eucalyptus, Lemongrass는 치석생성을 억제하는 천연소재로 구강관리 제품개발에 활용할 수 있는 가능성을 확인할 수 있었다.

• 아시안잔디학회지
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• 제24권2호
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• pp.191-198
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• 2010

골프장 잔디에서 효과적으로 지렁이를 추출하는 방법과 골프장의 잔디에 서식하는 지렁이의 종류를 알아보기 위하여 연구를 수행한 결과, 차나무추출물이 mustard oil이나 formalin에 비하여 붉은줄지렁이(Eisenia andrei)의 추출에 효과가 있었다. 그리고 차나무추출물 1000배 처리가 (4 L/m2)가 500배와 2000배 처리에 비하여 효과가 높았다. 토양 내 서식 깊이가 얕을수록 추출효과가 높았다. 그러나 차나무추출물 처리 후 추가적인 관수는 추출에 크게 영향을 미치지 않았다. 우리나라 10개 골프장에는 4속 8종의 지렁이가 분포하고 있었다. 그 중 변이성지렁이(Amynthas heteropodus)가 6개 골프장에서 147개체가 채집되어 우점종이었으며 염주위지렁이(Drawida japonica)가 3개 골프장에서 13개체가 채집되었고, 외무늬지렁이(A. hilgendorfi)와 똥지렁이(A. hupeiensis)가 2개 골프장에서 채집되었다. 차나무추출물로는 똥지렁이가 탈출되지 않았다.

• 한국식품영양학회지
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• 제35권5호
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• pp.313-323
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• 2022

To investigate antioxidant effects of tea tree root extracts using various extraction methods, cytotoxicity, DPPH and ABTS radical scavenging, SOD, nitrite scavenging activity and inhibitory activity of lipid peroxidation, reducing power, ferrous ion chelating activity were measured. Cytotoxicity for RAW 264.7 cells was not observed at concentrations treated with below 90 ㎍/mL in all extracts. The maximum DPPH radical, nitrite scavenging, SOD activity and inhibitory activity of lipid peroxidation were obtained at the ethylacetate and 70% ethanol extract. The maximum ABTS radical scavenging activity was obtained at the ethylacetate and hot water extract. However, in the case of reducing power and ferrous ion chelating activity, they were obtained at 70% ethanol and hexane extract, respectively. Nitrate scavenging activity showed the most excellent scavenging ability of 59.6% at 90 ㎍/mL of ethylacetate. The hexane extract had the highest ferrous ion chelating activity, showing 61.05% at 50 ㎍/mL, 66.07% at 70 ㎍/mL and 76.81% at 90 ㎍/mL, respectively. The results of this research show that the ethylacetate and 70% ethanol extracts of tea tree root can be used as a natural material for scavenging the radicals. However, future study is necessary to understand the mechanism of antioxidant activity by identification of substances.

• 한국식물병리학회지
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• 제12권4호
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• pp.463-465
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• 1996

1992∼1994년 전남 보성지방 다원에서 2번차 수확 및 전지후의 차나무에 겹둥근무늬병의 발생이 관찰되었다. 잎에는 다갈색의 원형 내지 부정형의 병반 위에 겹둥근무늬가 형성되었고, 후기에는 병반 위에 겹둥근무늬의 소혹점(분생자층)이 다수 형성되었다. 이 병원균은 Pestalotiopsis longiseta (Spegazzini) Dai et Kobayashi로 분리, 동정되었고, 균 접종 11일 후에 병징이 관찰되기 시작하였다.

• 한국패션뷰티학회지
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• 제3권3호
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• pp.31-40
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• 2005

The reason that aromatherapy began to draw attention again as the alternative therapy is because the essential oils that are used for aromatherapy not only allow the human body to maintain homeostasis but also have an effect on curing a mental feeling of fatigue and because these efficacies and effects are proceeding with being proved scientifically. Also, with coming to be high in a voice of concern about severally side effects that the modern medicine and the synthetic chemistry have, the aromatherapy came to be paid attention as the replacement therapy that can substitute for and supplement this. In particular, at the time of requiring a study on a substance to substitute for this after having been announced in Japan for 1998 a risk of Zincpyrithione that is being used as a component of dandruff treatment, it is thought that the aroma oil that was known for having an anti-dandruff effect has a merit of being easy in a user's access, thus it will be effective in curing the symptom of dandruff. Accordingly, this study conducted the following experiment in a bid to examine whether the aroma oil has the anti-microbial effect on the actual malassezia. First of all, it measured the minimum concentration of aroma oil in which the growth of malassezia is inhibited, by carrying out MIC(Minimum Inhibition Concentration) test in terms of anti-microbial activity against Malassezia furfur KCTC 7744 while using oils of Patchouli, Rosemary, Lavender, and Tea tree among aroma oils that are being used for dandruff treatment. As a result of that, Tea tree oil has the most excellent anti-microbial activity, and the measurement result of anti-microbial activity was shown in order of Lavender oil>Rosemary oil>Patchouli oil. Next, it carried out the is actually eased by applying Tea tree oil directly to the scalp of dandruff. In consequence of the experiment, the massage of Tea tree oil indicated the result of relieving the symptoms of dandruff such as scale, itch, pain, inflammation, and sebum, and among these symptoms of dandruff, it was shown to be most effective in easing itch. Especially, this clinical experiment was indicated that the massage of Tea tree oil has the most effect on relieving itch of targets with a dry-skin type who had no experience of dandruff treatment.

• 농업생명과학연구
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• 제45권5호
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• pp.1-8
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• 2011

In this study, 160 tea tree (Camellia sinensis L.) lines were classified by caffeine content using colorimetric methods. Among them, caffeine-rich lines (HR-78, HR-137, HR-82 and HR-123) and poor lines (HP-85, HP-88, HP-19, and HP-131) were selected. To know the difference in morphological and genetic characters between caffeine-rich and poor lines, we used leaf/shoot growth and RAPD methods. Cluster pattern of morphological characters (leaf width, leaf length, leaf area and shoot length) showed that shoot length was longer in caffein-rich lines than in -poor lines. In genetic analysis, amplified DNA bands having various sizes were detected in RAPD analysis where 30 random primers were used. However, the discriminated primer set that distinguish caffein-rich tree line from -poor lines was not found. These results can be used as the basic data to determine the morphological and genetic differences among caffein-rich and -poor lines.

• 치위생과학회지
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• 제16권6호
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• pp.409-414
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• 2016

본 연구의 목적은 천연 식물 중 허브의 일종인 티트리 성분이 함유된 의치세정제를 제조하고 레진의 표면을 분석하고 항균 효과를 평가하고자 하였으며, 다음과 같은 결과를 얻었다. 티트리 오일이 각각 30 vol%, 50 vol% 첨가된 실험군 의치세정제를 제조하였으며 실험군 티트리 오일에서 24시간, 48시간 보관한 후 표면 경도를 확인한 결과 대조군 생리식 염수와 유의한 차이가 없었으며, 시간에 따른 변화 역시 유의한 차이가 없었다. 의치상의 색상변화를 확인하기 위해 색차색도계를 이용하여 24시간, 48시간에 따른 전체변화량 ${\Delta}E^*$값을 확인한 결과 대조군 생리식염수와 유의한 차이가 없어 티트리 의치세정제가 의치표면의 색변화를 나타내지 않았다. 마지막으로 항균력을 평가한 결과 실험군인 티트리 50%군에서는 $3.18{\pm}2.59CFU/ml$로 원액과 유의차를 확인 할 수 없었지만 30%군보다는 높은 항균력을 보였다. 30%군에서는 $36.81{\pm}28.42CFU/ml$로 대조군보다는 높은 항균력을 보였지만 50%군과 티트리 원액 실험군보다는 낮은 항균력을 나타냈다. 따라서 티트리 오일이 함유된 의치세정제는 의치상용레진에 물리적 기계적 변화 없이 우수한 항진균력을 보여 향후 천연성분인 티트리 오일이 함유된 의치세정제 개발을 위한 기초 자료로 활용될 수 있을 것이라 생각된다.

• 한국식생활문화학회지
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• 제12권5호
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• pp.567-572
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• 1997

This reserch was carried out to investigate the nitrite scavenging abilities (NSA) of 7 kinds of tea extracts. Nitrites are used as additives of cured meat preperation and exist in plants, foods, and medicines, so we intake it very often easily. Nitrites can act with amines to produce nitrosamine which is known to be a carcinogen. It is known that the pH, concentration of amines, and amounts of nitrite are three important conditions of nitrosamine occurence. 7 kinds of tea used in this experiment were Persimmon tree (Diospyroo kaki Thiunb, Per.), Mulberry tree (Morus alba Linne, Mul.), Rubber tree (eucommia ulmoi-des Oliver, Rub.), Solomon's-seal (Bolygonatu Morr, Som.), Chicory (Cichorrium intybus L, Chi.), Sumach (Rhus javanica L., Sum.), Docwood (Cornus officinale Sieb, Doc.) and they were extracted with methanol (MeOH), ethyl ether (EtEt), ethyl acetate (EtAc), and also the extract existed in the aqueous layer II (Aq L. II) was used.