• Horticultural Science & Technology
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• v.32 no.3
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• pp.366-374
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• 2014

The study was conducted to investigate the possibility that epigenetic DNA methylation causes tree phenotypic variation in autotetraploids through evaluating the phenotypic variation and DNA methylation in autotetraploids occurred spontaneously from diploid trifoliate orange. Chromosome analysis confirmed that fourteen trifoliate orange trees of selected by flow cytometry were tetraploids (2n = 4X = 36) without any aneuploids. Chromomycin A3 staining determined that these trees were all autotetraploid with doubled chromosome set. Tree phenotypes, such as tree height and width, branching number, length, and angle, internode length, and leaf characteristics, varied in the autotetraploids. Chlorophyll indices were diverse in the autotetraploids, but photosynthetic rates were not significantly different. In addition, a wide range of variation was observed in stomatal density and guard cell length. Analysis of global cytosine DNA methylation showed that there was a variation of the methylation level in autotetraploids. More than half of 14 autotetraploids had at least 2 times higher methylation level than diploid trifoliate orange. The results indicate that tree phenotypic variation in autotetraploids might be related to global DNA methylation for reducing gene redundancy.

• Korean journal of applied entomology
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• v.34 no.3
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• pp.229-233
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• 1995

The life history, seasonal occurrence of larval population and natural enemies of Tea leaf roller (Caloptilia theivora (Walshingham) were investigated in field-age. C. theivora had 6 generations a year. The developmental periods from egg to adult emergence were 32.1~38.6 days in spring and fall, and 24.8~32.7 days in summer. The average longevities of adults were 8.4~14.5 days in spring and fall, and 6.3~8.6 days in summer. The average number of eggs laid by a female were 55~71 throughout the season. Larval population density of C. theivora showed 3~4 peaks from middle may in 1992 and 1993. However, population density of 194 was pretty low in early season and exhibited a peak in late September-early October. C. theivora overwintered in pupal stage on the leaves. Four hymenopterous parasitoids of C. theivora larvae were identified ; they are Stenomesius japonicus (Ashmead), Sympiesis ringoniellae Kamijo, Elasmus sp., and S. dolichogaster Ashmead which is dominant.

• Korean Journal of Medicinal Crop Science
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• v.13 no.1
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• pp.11-16
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• 2005

Soybean sprouts presoaked to leaf extracts were on sale as taking a growing concern about wellbeing foods. The study was carried out to compare the effects of culture period after presoaking tea (Thea sinensis L.) and hard rubber tree (Eucommia ulmoides Oliver) leaf extracts on lateral root formation, growth and morphological characters of the sprouts. Seeds of three cultivars, Pungsannamulkong, Sowonkong and Junjery, were imbibed for 5 hours into 10% solutions immediately before 5, 6 and 7 day cultures. The cultured sprouts were classified into 4 categories to calculate their composition rates on the base of hypocotyllengths; >7 cm (A),4 to 7 cm (B),< 4 cm (C) and not germinated (D), and their morphological characters, fresh and dry weights were measured. Rate of A was the lowest in Junjery of the three cultivars, while that of C showed reverse result compared to A. Similar results to that of A were observed between the three cultivars in sprout length including hypocotyl and root, diameters in middle and hook parts of hypocotyl, total fresh and dry weights. Regardless of tea and hard rubber tree leaf extract treatments, longer culture periods increased rate of A due to decrements of B, C and D, and also did the growth and the morphological characters mentioned above. Although longer culture periods reduced the increment of lateral roots, the latter treatment was more delayed in their formation and less in the roots per sprout than the former one. The growth and the morphological characters above were less in the latter treatment than in the former one in general, meaning that hard rubber tree leaf extract could more repress growth of soybean sprouts than tea leaf extract.

• Korean Journal of Medicinal Crop Science
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• v.13 no.1
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• pp.6-10
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• 2005

Lateral roots of soybean sprouts should deeply affect their quality and marketability. The study was done to compare the effects of ocher, chitosan, corn kennel, tea (Thea sinensis L.) and hard rubber tree (Eucommia ulmoides Oliver) leaf extracts on lateral root formation, growth and morphological characters of the sprouts. Seeds of three cultivars, Pungsannamulkong, Sowonkong and Junjery, were imbibed for 5 hours into their 10% solutions and cultured for 6 days. The cultured sprouts were classified into 4 categories to calculate their composition rates on the base of hypocotyl lengths;>7 cm (A), 4 to 7 cm (B), < 4 cm (C) and not germinated (D), and their morphological characters, fresh and dry weights were measured. Composition rate of A was the lowest in Junjery of the three cultivars, while that of C showed reverse result compared to A. This results was the most distinct in hard rubber tree leaf extracts (HRTLE) of the five treatments. In HRTLE treatment, lateral root formation rate were formed in almost of Sowonkong although reduced in order of Pungsannamulkong and Junjery. However, there was no significant difference between the other treatments. Lateral roots per sprout were the lowest in HRTLE treatment of the 5 treatments. In all treatments except the chitosan treatment, the roots were most formed in Sowonkong but least in Junjery. Sprout length adding hypocotyl and root was the shortest in Junjery compared to the other two cultivars. and was the longest in tea leaf extract treatment but the shortest in HRTLE treatment. The result in total fresh weight of sprouts was similar to that of the sprout length.

• Journal of Life Science
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• v.24 no.3
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• pp.266-273
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• 2014

In the present study, the chemical compositions and antimicrobial and antioxidant activities of commercially available essential oils in Korea were investigated. The essential oils were analyzed by gas chromatography-mass spectrometry (GC-MS). The results demonstrated that they exhibit a broad spectrum of antimicrobial activities against gram positive, gram negative, and multidrug resistant (MDR) strains. The antimicrobial activity of five of the essential oils against 40 organisms was assessed using the minimum inhibitory concentration (MIC). The MIC values were in the ranges of 0.0625-0.5% (v/w) for lemongrass and manuka, 0.03125-1.0% (v/w) for tea tree, 0.0625-1.0% (v/w) for thyme, and 1-4% (v/w) for ravensara, depending on the pathogens studied. This study revealed that, among the essential oils tested, lemongrass and thyme oil showed broad antimicrobial activity against infectious bacteria. The antioxidant activities and the reducing power of the essential oils were determined with a 1, 1-diphenyl-2-picrylhydrazyl (DPPH) assay. Thyme oil exhibited the strongest antioxidant activity comparing with ascorbic acid. This is the first report on the chemical compositions and antimicrobial activities of commercially available essential oils against infectious bacteria and MDR strains acquired from Korean hospitals.

• Journal of Life Science
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• v.22 no.10
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• pp.1344-1351
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• 2012

We studied the physical, chemical, biological, and antimicrobial effects of eight types of essential oils used in the cosmetics industry: lavender, tea tree, rosemary, juniper berry, Chamaecyparis obtusa, cypress, cedar wood, and pine. Lavender oil had a linalyl acetate (an ester chemical compound) content of 48% and radical scavenging activity of 22.36% at 5,000 ppm. Tea tree oil had radical scavenging activity of 43.94% at 5,000 ppm and antimicrobial activity against S. aureus, S. epidermidis, S. mutans, and C. albicans in each 6, 3.5, 6.5, and 5 mm, respectively. Chamaecyparis obtusa oil had the highest acidity (pH 2.64) compared with the other oils, and sesquiterpene compounds were found to have 19.20%. Cedar wood oil had the highest specific gravity and refractive index compared to the other oils and had a sesquiterpene content of 99.73%. The radical scavenging activity of cedar wood essential oil exceeded 39.68% at 5,000 ppm. The clear zone, indicating antimicrobial activity against P. acnes, P. ovale, and C. albicans, was 3.5, 6, and 6 mm, respectively, at a concentration of 1% cedar wood oil. Results showed that with a high sesquiterpene content, the antioxidant effect was generally, but not always, high, suggesting that this is determined according to composition of the compound rather than presence of each antioxidant. The results indicate that antimicrobial activity is determined by the existence of each antimicrobial ingredient rather than terpene composition.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.3
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• pp.1139-1145
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• 2010

This thesis tried applying Ayurvedic hair and scalp care program and analyzing its effect. Ayurvedic treatment strengthens our immunity through natural healing power. I measured with MIC test and Halo test in order to examine the antibacterial efficacy against colonies which reside in head, when Ayurvedic base oils and essential oils were used on hair and scalp. Finally, I reached the results as follows. I examined the antibacterial efficacy of base oils and essential oils against Bacillus subtilis and Propionibacterium acnes, based on the MIC test with 9 kinds of base oil and 18 kinds of essential oil. By way of experiment, base oils did not show the antibacterial efficacy from all colonies, and in case of essential oils, such as Chamomile, Clary sage, Jasmine, Neroli and Rose oil, the antibacterial efficacy against two colonies did not appear by the measure of below 5%. It turned out that Thyme, Geranium, Lavender and Tea tree had an high effect on two colonies. According to the Halo test which experimented on 7 kinds of bacteria with the essential oils like Geranium, Lavender and Thyme and with the base oils like Sesame and Coconut, it went to prove that base oils was inefficient on antibiosis. It was examined that all of essential oils had the effect on antibiosis from 7 kinds of bacteria. Thyme showed the best efficacy of antibiosis in the MIC and the Halo test.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.29 no.1
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• pp.131-141
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• 2011

This study, as a purpose of base study for landscape redevelopement of Seowon, pointed out problems of planting patterns in each sector and suggested an alternative through analyzing old literature, complete enumeration survey in each sector and taking aerial images. the conclusions drawn from this study are as follows. A Pilam Seowon(historic site no 242) is located in a Pyungya-Sanrok(plain and mountain) district in Jangsung-gun and has Junhak-Humyo(Study room in the front and Shirne in the back) type. 23 taxonomic group of arbor, 6 taxonomic group of shrub and 5 taxonomic group of flowers are planted high-densitily in limited flat surface. It can be classified into being planted by family and being planted through landscape redevelopement in 1980s. Korean traditional trees are planted in this area like Pinus densiflora, Juniperus chinensis L., Thuja orientalis L., Zelkova serrato Makino, Sophora japonica L., Lagerstroemia indica, Prunus mume include Ginkgo biloba which is old big tree and is registered in Jangsung-gun as protected trees. The tea tree in this area was introduced from wild tea plantation in Yonhwa moutain and was planted. From now on, we have to pay consideration a form management of planted trees in the historical area, rearrangement of plant density due to over planting, removal of alien trees like Pinus bungeana and Canna generalisa, prevention a dwarfishness of main area due to over planting in outside facilities, recovery a alienation among each planting area, and planting concept from plants drawings on wall of buildings can be suggested. The implications of this case study is that introduction of over planting can make more problems like losing sense of place. Therefore, we must do a actual state survey on traditional landscape area like Seowon from preservation point of view.

• Journal of the Korean Society of Food Culture
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• v.26 no.1
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• pp.39-52
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• 2011

This study examined the types and cooking methods for dasik (traditional pressed sweet), as recorded in 16 old Joseon dynasty (1392-1909) studies. The ingredients used in dasik during the Joseon dynasty were categorized into cereal powders, tree fruits, flower powders, root clods, dry-fish beef powders, and vegetables. In the early, middle, and late eras of the Joseon dynasty, $1^{st}$ set (two, five, and 70 kinds of dasik), $2^{nd}$ set (two, four, and 16 kinds of dasik) were prepared using cereal powders, and during the middle and late eras of the Joseon dynasty one and 22 kinds of dasik were prepared using tree fruits. During the late eras of the Joseon dynasty, seven kinds of dasik were prepared using flower powders, 11 kinds of dasik were prepared using root clods, 12 kinds of dasik were prepared using dry-fish beef powders, and two kinds of dasik were prepared using vegetables. The frequency of the ingredients were in the order of Huek-im (黑荏), Hwang-yul (黃栗), Jin-mal (眞末), Song-wha (松花), and Nok-mal (菉末) during the Joseon dynasty. To prepare dasik, the ingredients were mixed with honey, soy sauce, sesame oil, pepper, and water or ground and shredded to prepare for pressing and for abstract dasik, respectively. The appearance and taste of dasik varied, thereby resulting in nutrient supplementation, as the types of ingredients increased throughout the Joseon dynasty. This observation may be associated with the commercial industrial development that prevailed during the late Joseon dynasty. Further investigation will be conducted on the recipes and ingredients recorded in these old studies to develop a standardized recipe for the globalization of dasik.

• Journal of the Korean Burn Society
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• v.24 no.1
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• pp.7-13
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• 2021

Purpose: Currently, the most recommended method for burn first aid is the cool running water for 20 minutes (CRW20). If CRW20 is not practicable, cooling dressing products with tea tree oil are used as substitutes. In this study, we analyze the effect of various burn first aids, including CRW20, cooling products, on the treatment period. Methods: This study was conducted on patients who suffered burns and visited Hangangsoo Hospital from March 2019 to March 2020. The study conducted in a retrospective method. The duration of treatment was analyzed by dividing the experimental group. Results: The treatment period was shortened when first aid was given (P<0.001). Cold water, ice, and ice packs resulted in reduced treatment periods (P<0.001, P=0.004). The treatment period was reduced when cooling dressing products were used in all groups (P=0.041). The implementation of first aid has reduced the duration of burn treatment. This means that cooling helps burn treatment. CRW20 did not show statistically meaningful results. This is the result of a failure to fully control the temperature and time of tap water. In addition, the change in temperature of tap water according to season, the use of water is higher than that of animal testing, and the general lack of information on proper burn first aid may have affected. Conclusion: The treatment period was statistically significantly reduced in the group that performed first aid. Among the various first aid methods, cold water and ice reduced the treatment period.