• Korean Journal of Veterinary Research
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• v.9 no.1
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• pp.63-69
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• 1969

Various methods for the isolation of salmonellae were compared by examining mesenteric lymph nodes and faeces of 395 pigs at slaughter. The conclusions were as follows. (i) Tetrathionate broth was significantly superior to selenite broth for the examination of mesenteric lymph nodes. (ii) There was no significant difference between tetrathionate broth and selenite broth for the examination of faeces. The use of both tetrathionate broth and selenite broth as enrichment media for faeces produced far better results than the use of any single enrichment medium. (iii) Brilliant green agar was significantly superior to SS agar for the examination of mesenteric lymph nodes. (iv) There was no significant difference between brilliant green agar and SS agar when faeces were examined. The use of both media produced far better results than the use of either singly. (v) Brilliant green MacConkey broth was much inferior to tetrathionate broth as an enrichment broth. Direct culture of faeces on brilliant green MacConkey agar yielded less isolates than prior enrichment in tetrathionate or selenite broth. (vi) The optimum incubation period of enrichment was 24 hours for faeces and 48 hours for mesenteric lymph nodes when either tetrathionate broth or selenite broth was used as enrichment media.

• Bulletin of the Korean Chemical Society
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• v.31 no.5
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• pp.1283-1288
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• 2010

A novel polyaminopolycarboxylate ligand with many coordination sites, N,N,$N^1,N^1,N^2,N^2$-[( 2,4,6-tri(aminomethyl)-pyridine]hexakis(acetic acid) (TPHA), was designed and synthesized and its lanthanide complexes $Na_6Tb_2$(TPHA)$Cl_6{\cdot}14H_2O$, $Na_6Eu_2$(TPHA)$Cl_6{\cdot}8H_2O$, $Na_6Gd_2$(TPHA)$Cl_6{\cdot}11H_2O$ and $Na_6Sm_2$(TPHA)$Cl_6{\cdot}9H_2O$ were successfully prepared. The ligand and the complexes were characterized by elemental analysis, IR, mass, NMR and TG-DTA. The TG-DTA studies indicated that the complexes had a high thermal stability, whose initial decomposition temperature was over $270^{\circ}C$. The luminescence properties of the complexes in solid state were investigated and the results suggested that $Tb^{3+}$ and $Eu^{3+}$ ions could be sensitized efficiently by the ligand, especially the Tb(III) complex displayed a very strong luminescence intensity (> 10000) and only displayed characteristic metal-centered luminescence. Also, the correlative comparison between the structure of ligand and luminescence properties showed how the number of the coordination atoms of ligand can be a prominent factor in the effectiveness of ligand-to-metal energy transfer.

• Korean Journal of Veterinary Research
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• v.43 no.1
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• pp.25-29
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• 2003

This study was carried out to investigate a usefulness of the microsatellite DNA markers for parentage verification of Thoroughbred (TB) horses. 9 TB horses samples were genotyped for nine international minimum standard markers (AHT4, 5, ASB2, HMS3, 6, 7, HTG4, 10, and VHL20), and the additional panel of four markers, ASB17, CA425, LEX33, and TKY321. This methods consisted of multiplexing PCR procedures, and it showed reasonable amplification of all PCR products. Genotyping was performed with an ABI 310 genetic analyzer. Foal I was excluded according to principles of Mendelian genetics in AHT4 (H/K), ASB2 (Q/Q), HMS3 (I/P), HTG4 (M/O), HTG1O (K/R), VHL20 (M/P), ASB17 (F/N), LEX33 (M/O), and TKY321 (G/I) markets. Foal II was excluded with markers AHT5 (K/M), ASB2 (M/N), HMS7 (N/N), HTG1O (K/K), VHL20 (I/I), ASB17 (F/F) and TKY321 (G/I). Foal III was excluded with markers AHT4 (O/O), AHT5 (K/K), ASB2 (M/R), HMS6 (M/P), HMS7 (O/O), HTG10 (R/S), VHL20 (L/M), and ASB17 (N/O). These results suggest that the present DNA typing is so useful for parentage verification of TB horses.

• 한국전산유체공학회:학술대회논문집
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• 2006.05a
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• pp.275-278
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• 2006

Japan Aerospace Exploration Agency has introduced a new terascale clusterd SMP system as a main compute engine of Numerical Simulator III for aerospace science and engineering research purposes. The system is using Fujitsu PRIMEPOWER HPC2500; it has computing capability of 9.3Tflop/s peak performance and 3.6TB of user memory, with about 1,800 scalar processors for computation. In this paper, we first present the performance evaluation results for aerospace CFD applications with hybrid programming paradigm used at JAXA. Next we propose a performance prediction formula for hybrid codes based on a simple extension of AMhhal's law, and discuss about the predicted and measured performances for some typical hybrid CFD codes.

• The Korean Journal of Physiology and Pharmacology
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• v.16 no.2
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• pp.125-130
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• 2012

Alcohol abuse and its medical and social consequences are a major health problem in many areas of the world. The present study was conducted to evaluate the protective effect of methanolic fruit extract of $Randia$ $Dumetorum$ (L.) on alcohol-induced liver damage in rats. Rats were divided into five different groups (n=6), group I served as a control, group II received ethanol (3 ml/100 g/day p.o.), group III served as standard group and received silymarin (50 mg/kg p.o.), group IV and V served as extract treatment groups and received 50 & 100 mg/kg methanolic extract of $R.$ $dumetorum$. All the treatment protocols followed 30 days and after rats were sacrificed blood and liver were used for biochemical and histological studies, respectively. The activities serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), direct bilirubin (DB), total bilirubin (TB) and lipid peroxidation were statistically increased in rats exposed to alcohol while total protein and glutathione decreased compared to control rats. Treatment with $R.$ $dumetorum$ significantly decreased the elevated levels of ALT, AST, TG, DB, TB and lipid peroxidation compared to the group exposed to alcohol only. $R.$ $dumetorum$ significantly resulted in increased levels of total protein and reduced glutathione compared to the group that received alcohol only. Histology of the liver section of the animals treated with $R.$ $dumetorum$ improved the hepatotoxicity caused by alcohol. Hence the study concluded that $R.$ $dumetorum$ has potential hepatoprotective activity.

• Bulletin of the Korean Chemical Society
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• v.34 no.12
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• pp.3609-3614
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• 2013

Orthorhombic and hexagonal lanthanum(III) hydroxycarbonate ($LaCO_3OH$) and $Ln^{3+}$-doped $LaCO_3OH$ ($LaCO_3OH:Ln^{3+}$, where Ln = Ce, Eu, Tb, and Ho) powders were prepared by a hydrothermal reaction under ambient pressure and characterized by thermogravimetry, powder X-ray diffraction, infrared and luminescence spectroscopy, and field-emission scanning electron microscopy. The polymorph of $LaCO_3OH$ depended on the reaction temperature, inorganic salt additive, species of $Ln^{3+}$ dopant, and solvent. The calcination of orthorhombic $LaCO_3OH:Ln^{3+}$ (2 mol %) powers at $600^{\circ}C$ yielded a mixture of hexagonal and monoclinic $La_2O_2CO_3:Ln^{3+}$ powders. The relative quantity of the latter increased with decreasing ionic radius of the $Ln^{3+}$ dopant ion and increasing doping concentrations. On the other hand, the calcination of hexagonal $LaCO_3OH:Ln^{3+}$ (2 mol %) powders at $600^{\circ}C$ resulted in a pure hexagonal $La_2O_2CO_3:Ln^{3+}$ powder, regardless of the species of $Ln^{3+}$ ions (Ln = Ce, Eu, and Tb). The luminescence spectra of $LaCO_3OH:Ln^{3+}$ and $La_2O_2CO_3:Ln^{3+}$ were measured to examine the effect of their polymorph on the spectra.

• Journal of the Korean Vacuum Society
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• v.20 no.6
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• pp.461-467
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• 2011

The valence band maximum and the conduction band miminum of GaAs, GaSb, InAs, and InSb (constituent binaries of the quaternaty alloy $Ga_xIn_{1-x}Sb_yAs_{1-y}$) are calculated by using TB analytical approach method. The band alignment types of their heterojunctions are determined directly from their relative position of band edges (VBM and CBM). For example, the GaAs/InAs, GaAs/InSb, and GaSb/InSb are in a type-I, the GaAs/GaSb in a type-II, and the GaSb/InAs and InSb/InAs in a type-III, respectively. The composition dependent VBM and CBM for the $Ga_xIn_{1-x}Sb_yAs_{1-y}$ alloy are obtained by using the univeral tight binding method. For the alloyed heterojunctions, the band alignments can be controlled by changing the composition which induce a band type transition. For the alloy $Ga_xIn_{1-x}Sb_yAs_{1-y}$ lattice mathced to GaSb, the type-II band alignment in the region of $x{\leq}0.15$ is changed to the type-III in the region of $x{\geq}0.81$. On the other hand, the alloy $Ga_xIn_{1-x}Sb_yAs_{1-y}$ lattice mathced to InAs has the type-II band alignment in the region of $x{\leq}0.15$ and the type-III band alignment in the region of $x{\geq}0.81$, respectively.

• The Korean Journal of Zoology
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• v.32 no.2
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• pp.120-133
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• 1989

The paraidehyde-fuchsin(PAE)-positive neurosecretory cells in the brains of wax moth Galleria mellonella have been morphologically examined at the six different metamorphic stages. During the metamorphosis, neurosecretory cells in the brain can be found in the five unclei of pars intercerebralis, lateral region of protocerebrum, optic lobe, deutocrebrum and tritocerebrum. The five nuelel include one to seven neurosecretory cells. On the bases of cell sizes and histochemical specificities of neurosercretion within cells, all the PAF-positive neurosercretory cells included in the six different metamorphic brains can be recognized as four species of neurosecretory cells as follows; (1) large (about 25 $\mu$m), neurosecretion-parcked cell (type I neurosecretory cell), (2) large, granule-dispersed cell (type II neurosecretury cell), (3) small (about 15 $\mu$m), neurosecretion-packed cell (type III neurosecretory cell), and (4) small, granule-dispersed cell (type IV neurose-cretory cell). The three tb seven medial neurosecretory cells are included in the pars intercerebralis of the six different metamorphic brains. With the increase of days from the late larva to the adult the type I cells of medial neurosecretory cells gradually decrease in number, but the respective three type II neurosecretory cells appear in the five different metamorphic brains except in pupa 2 day before the emeregnce of the adult which has only one type II. The one to five lateral neurosecretory cells are observed in the lateral region of protocerebrum from thepupa just after pupation to the adult. The type IV neurosecretory cells are the most in number of lateral neurosecretory cells. The one type Ineurosecretory cells are included near the optic lobe of only the 4-day-old pupa. the one deutocerebral neurosecretory cell, type II, appears only in the adult. The tritocerebrum includes both three neurosecretory cells in the late larva and one neuresecretory cell in the adult. In the late larva the two tritocerebral neurosecretory cells are type Ill neurosecretory cell and the one is type IV. The remaining one tritocerebral neurosecretory cell is type IV.

• The Korean Journal of Food And Nutrition
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• v.15 no.2
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• pp.112-118
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• 2002

Sporulation in the fission yeast Schizosaccharomyces pombe has been regarded as an important model of cellular development and differentiation. S. pombe cells proliferate by mitosis and binary fission on growth medium. Deprivation of nutrients especially nitrogen sources, causes the cessation of mitosis and initiates sexual reproduction by matting between two sexually compatible cell types. Meiosis is then followed in a diploid cell in the absence of nitrogen source. DNA fragment complemented with the mutations of sporulation gene was isolated from the S. pombe gene library constructed in the vector, pDB 248' and designated as pDB(spo5)1. We futher analyzed six recombinant plasmids, pDB(spo5)2, pDB(spo5)3, pDB(spo5)4, pDB(spo5)5, pDB (spo5)6, pDB(spo5)7 and found each of these plasmids is able to rescue the spo5-2, spo5-3, spo5-4, spo5-5, spo5-6, spo5-7 mutations, respectively. Mapping of the integrated plasmid into the homologous site of the S. pombe chromosomes demonstrated that pDB(spo5)1, and pDB(spu5)Rl contained the spo5 gene. Transcripts of spo5 gene were analyzed by Northern hybridization. Two transcripts of 3.2 kb and 2.5kb were detected with 5kb Hind Ⅲ fragment containing a part of the spo5 gene as a probe. The small mRNA(2.5kb) appeared only when a wild-type strain was cultured in the absence of nitrogen source in which condition the large mRNA (3.2kb) was produced constitutively. Appearance of a 2.5kb spo5-mRNA depends upon the function of the meil, mei2 and mei3 genes.