• Parasites, Hosts and Diseases
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• 제55권6호
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• pp.623-630
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• 2017

Plasmodium lactate dehydrogenase (pLDH) is a strong target antigen for the determination of infection with Plasmodium species specifically. However, a more effective antibody is needed because of the low sensitivity of the current antibody in many immunological diagnostic assays. In this study, recombinant Plasmodium vivax LDH (PvLDH) was experimentally constructed and expressed as a native antigen to develop an effective P. vivax-specific monoclonal antibody (mAb). Two mAbs (2CF5 and 1G10) were tested using ELISA and immunofluorescence assays (IFA), as both demonstrated reactivity against pLDH antigen. Of the 2 antibodies, 2CF5 was not able to detect P. falciparum, suggesting that it might possess P. vivax-specificity. The detection limit for a pair of 2 mAbs-linked sandwich ELISA was 31.3 ng/ml of the recombinant antigen. The P. vivax-specific performance of mAbs-linked ELISA was confirmed by in vitro-cultured P. falciparum and P. vivax-infected patient blood samples. In conclusion, the 2 new antibodies possessed the potential to detect P. vivax and will be useful in immunoassay.

• 한국균학회지
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• 제41권1호
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• pp.52-55
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• 2013

Phoma glomerata는 식물 잎이나 열매에 병을 일으키는 식물병원균으로 알려져 있다. 국내에서는 아직 피해사례가 없기 때문에 P. glomerata는 국내의 식물검역균으로 관리되고 있다. 본 연구는 국내에 들어오는 목재나 과일에 P. glomerata를 검출할 수 있는 방법 개발코자 수행되었다. Phoma 균주들의 translation elongation factor 1 alpha 유전자 염기서열에 기초하여 P. glomerata 특이적 PCR 프라이머를 디자인 하였고 그 특이성을 검정하였다. PCR 수행 결과 P. glomerata에서만 170 bp 크기의 밴드가 증폭되었고, 다른 비교 균주에서는 밴드가 증폭되지 않았다. 검출 감도를 평가하기 위해 기존 PCR방법과 real time PCR 방법을 이용하여 실험한 결과 최소 10 pg과 1 pg까지 각각 검출할 수 있었다. 본 연구결과는 디자인된 PCR 프라이머가 P. glomerata를 특이적으로 검출하는데 유용할 것임을 보여준다.

• 한국콘텐츠학회논문지
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• 제13권10호
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• pp.54-61
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• 2013

본 논문에서는 하드웨어 기반의 영상 신호 윤곽선 검출을 위한 하드웨어기반의 알고리즘으로 CMOS 이미지 센서의 인접픽셀과 아날로그 상관기로 구성되는 윤곽선 검출기를 제안한다. 제안하는 이미지 윤곽 검출기는 각 열(column)마다 비교기를 공유하고, 비교기는 기준전압과 비교를 통해 대상 픽셀의 윤곽선 여부를 판별한다. 이미지 센서와 직접적으로 연결된 윤곽선 검출 회로는 기존의 연구와 비교하여 면적은 4배, 그리고 전력소모는 20 % 감소하는 결과를 보였다. 또한 외부에서 기준전압을 제어할 수 있어, 윤곽선 검출의 민감도를 조절하기에 유용한 장점을 가진다. 0.18 ${\mu}m$ CMOS 공정에서 제작된 칩은 34%의 fill factor를 가지며, 픽셀 당 0.9 ${\mu}W$의 전력소모를 가진다.

• BMB Reports
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• 제44권9호
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• pp.601-606
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• 2011

HMGB1 is associated with human cancers and is an activator of autophagy which mediates chemotherapy resistance. We here show that the mRNA levels of HMGB1 are high in leukemia cells and it is involved in the progression of childhood chronic myeloid leukemia (CML). HMGB1 decreases the sensitivity of human myeloid leukemia cells K562 to anti-cancer drug induced death through up-regulating the autophagy pathway, which is confirmed by the observation with an increase in fusion of autophagosomes and autophagolysosomes. When overexpressing HMGB1, both mRNA levels of Beclin-1, VSP34 and UVRAG which are key genes involved in mammalian autophagy and protein levels of p-Bcl-2 and LC3-II are increased. Luciferase assays document that over-expression of HMGB1 increases the transcriptional activity of JNK and ERK, which may be silenced by siRNA. The results suggest that HMGB1 regulates JNK and ERK required for autophagy, which provides a potential drug target for therapeutic interventions in childhood CML.

• The Plant Pathology Journal
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• 제20권4호
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• pp.252-257
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• 2004

We developed a polyclonal antibody based-ELISA system to monitor inocula accurately and rapidly before onset of anthracnose on soybean sprouts. Titer of mouse antisera against conidia of Colletotrichum gloeosporioides, determined by indirect ELISA, was high enough to be detectable up to ${\times}$25,600 dilutions. Both PAb1 and PAb2 had the highest level of reactivity to Colletotrichum gloeosporioides. Absorbance readings exceeded 0.15. Sensitivity of PAb to C. gloeosporioides was precise enough to detect spore concentration as low as 500 conidia/well by indirect ELISA. Both antibodies are very sensitive and highly specific to the target pathogen Colletotrichum gloeosporioides, apparently discriminating other unrelated pathogen, or epiphytes. This kit fulfills the requirements far detecting inocula before infection and onset of anthracnose. Our ELISA system should also be feasible to detect C. acutatum (Mungbean sprouts rot) and G. cingulata (C. gleosporioides), (apple, pepper). It was remarkable that absorbance value was not reduced even after 4 consecutive washings (Fig.4), suggesting that antigenic determinants are on the surface of conidia. Antigenic determinant was characterized by heating and enzyme treatment: Both PAb1 and PAb2 bind to protein epitope that does not contain residue of amino acid, arginine, and Iysine, even though more work needs to be done.

• The Plant Pathology Journal
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• 제36권5호
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• pp.459-467
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• 2020

Areca palm yellow leaf (AYL) disease caused by the 16SrI group phytoplasma is a serious threat to the development of the Areca palm industry in China. The 16S rRNA gene sequence was utilized to establish a rapid and efficient detection system efficient for the 16SrI-B subgroup AYL phytoplasma in China by loop-mediated isothermal amplification (LAMP). The results showed that two sets of LAMP detection primers, 16SrDNA-2 and 16SrDNA-3, were efficient for 16SrI-B subgroup AYL phytoplasma in China, with positive results appearing under reaction conditions of 64℃ for 40 min. The lowest detection limit for the two LAMP detection assays was the same at 200 ag/μl, namely approximately 53 copies/μl of the target fragments. Phytoplasma was detected in all AYL disease samples from Baoting, Tunchang, and Wanning counties in Hainan province using the two sets of LAMP primers 16SrDNA-2 and 16SrDNA-3, whereas no phytoplasma was detected in the negative control. The LAMP method established in this study with comparatively high sensitivity and stability, provides reliable results that could be visually detected, making it suitable for application and research in rapid diagnosis of AYL disease, detection of seedlings with the pathogen and breeding of disease-resistant Areca palm varieties.

• Journal of Applied Biological Chemistry
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• 제58권4호
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• pp.383-387
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• 2015

국내 유통 식품 수입 식품 중 토끼와 고양이 고기의 혼입 여부를 알아내고 불법 도축된 고양이 고기를 토끼 고기나 다른 고기로 속여 판매하는 것을 방지하기 위해 토끼와 고양이를 동시에 검출할 수 있는 polymerase chain reaction (PCR) 법을 개발하였다. 토끼와 고양이의 종 특이 프라이머는 미토콘드리아의 cytochrome b 유전자를 대상으로 하였고 개발된 프라이머를 가공식품에 활용하는 것을 고려하여 PCR 산물의 크기는 토끼 101 bp, 고양이 191 bp로 최소화 하였다. 프라이머의 특이성은 총 21종의 동물을 대상으로 검토하였다. 개발된 검출법의 검출 한계는 시료 DNA를 희석하여 PCR과 Bioanalyzer로 확인한 결과 토끼는 0.005 ng, 고양이는 0.0005 ng이었다.

• 한국농공학회논문집
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• 제60권6호
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• pp.33-42
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• 2018

This study evaluated the vulnerability of irrigation water supplied to the crops. The target areas were selected as Dangjin-si, Yesan-gun, and Cheongyang-gun. The survey items of the climate exposure were annual precipitation and rainless days. The sensitivity survey items were cultivation area, groundwater level, evapotranspiration and groundwater consumption. The survey items of the adaptability were Number of groundwater well and Water supply ratio. The survey methods for these items were investigated in a variety of ways, including "National Climate Data Service System", "Korean Statistical Information Service", "National ground water monitoring network in korea annual report" and "Chungcheongnam-do Statistical Yearbook", "HOMWRS". Vulnerability assessment results were rated within the range of 0~100 points. The first grade was rated 0-25, the second grade 26-50, the third grade 51-75, and the fourth grade 76-100. And the lower the score, the lower the vulnerability. As a result, Cheongyang-gun showed a high vulnerability of over 50 points, Dangjin-si showed a low vulnerability rating of 31.20 points and a Yesan-gun of 36.00 points.

• 대한불안의학회지
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• 제13권2호
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• pp.53-59
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• 2017

Reliable and valid diagnostic screening tools in the primary care setting enable accurate estimation of depression in individuals at risk or in need of treatment, and provide patients with an opportunity to receive optimal treatments. Although there have been extensive studies on screening tools for depression used in domestic mental health settings, there is little consideration and lack of a thorough review of the diagnostic validity of screening tools. In the current review, we selected 13 representative screening tools for depression which were evaluated in a total of 19 validation studies conducted in Korea. We summarized DSM-5 target domains, diagnostic indices, sensitivity, specificity, cut-off scores, and diagnostic validity information for each tool. Finally, the depression measurement expert group was constituted to evaluate the current status of screening tools for depression, and their recommendations for a new screening tool were summarized. This study was conducted as part of the Mental Health Technology Development project to develop the Korean screening tool for depression (K-DEP).

• 한국항행학회논문지
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• 제13권4호
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• pp.482-489
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• 2009

이 논문에서는 AWGN환경에서 발생하는 가우시안 잡음과 발진기에서 생기는 시스템 잡음을 제거할 수 있는 ALE(Adaptive Line Enhancer) 기반의 새로운 적응형 잡음 제거기를 이용한 CW(Continuous-Wave) 바이오 레이더를 제안한다. 최근에 CW 바이오 레이더를 이용해서 심장박동과 호흡을 검출하는 연구가 여러 연구기관에서 진행 되고 있다. 그러나 이 연구들은 기존 CW 바이오 레이더가 가우시안 잡음에 취약하고 그로 인해 심장박동 검출정확도도 떨어진다는 점을 설명을 하고 있긴 하지만, 그 잡음을 효과적으로 없앨 수 있는 방안은 계속 연구 중에 있다. 본 논문에서는 기저대역 신호에 포함된 잡음을 효과적으로 제거할 수 있는 ALE기반의 적응형 잡음 제거기를 적용한 것을 제안한다. 또한 타겟의 위치에 따른 복조의 민감함에 강점을 가진 quadrature 수신기를 통과한 잡음이 포함된 기저대역 신호에서 잡음만을 효과적으로 제거함으로 인해 심장박동 검출 정확도를 향상시키는 것을 모의실험을 통해 비교 분석해 본다.