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http://dx.doi.org/10.3347/kjp.2017.55.6.623

Development of Monoclonal Antibodies for Diagnosis of Plasmodium vivax  

Linh, Nguyen Thi Phuong (Zoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University)
Park, Hyun (Zoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University)
Lee, Jinyoung (Zoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University)
Liu, Dong-Xu (Zoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University)
Seo, Ga-Eun (Department of Microbiology, Ajou University School of Medicine, and Department of Biomedical Science, Graduate School of Ajou University)
Sohn, Hae-Jin (Department of Microbiology, Ajou University School of Medicine, and Department of Biomedical Science, Graduate School of Ajou University)
Han, Jin-Hee (Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University)
Han, Eun-Taek (Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University)
Shin, Ho-Joon (Department of Microbiology, Ajou University School of Medicine, and Department of Biomedical Science, Graduate School of Ajou University)
Yeo, Seon-Ju (Zoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University)
Publication Information
Parasites, Hosts and Diseases / v.55, no.6, 2017 , pp. 623-630 More about this Journal
Abstract
Plasmodium lactate dehydrogenase (pLDH) is a strong target antigen for the determination of infection with Plasmodium species specifically. However, a more effective antibody is needed because of the low sensitivity of the current antibody in many immunological diagnostic assays. In this study, recombinant Plasmodium vivax LDH (PvLDH) was experimentally constructed and expressed as a native antigen to develop an effective P. vivax-specific monoclonal antibody (mAb). Two mAbs (2CF5 and 1G10) were tested using ELISA and immunofluorescence assays (IFA), as both demonstrated reactivity against pLDH antigen. Of the 2 antibodies, 2CF5 was not able to detect P. falciparum, suggesting that it might possess P. vivax-specificity. The detection limit for a pair of 2 mAbs-linked sandwich ELISA was 31.3 ng/ml of the recombinant antigen. The P. vivax-specific performance of mAbs-linked ELISA was confirmed by in vitro-cultured P. falciparum and P. vivax-infected patient blood samples. In conclusion, the 2 new antibodies possessed the potential to detect P. vivax and will be useful in immunoassay.
Keywords
Plasmodium vivax; Plasmodium lactate dehydrogenase; monoclonal antibody;
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1 Baird JK, Valecha N, Duparc S, White NJ, Price RN. Diagnosis and treatment of Plasmodium vivax malaria. Am J Trop Med Hyg 2016; 95: 35-51.
2 Verma P, Biswas S, Mohan T, Ali S, Rao DN. Detection of histidine rich protein and lactate dehydrogenase of Plasmodium falciparum in malaria patients by sandwich ELISA using in-house reagents. Indian J Med Res 2013; 138: 977-987.
3 Dzakah EE, Kang K, Ni C, Wang H, Wu P, Tang S, Wang J, Wang J, Wang X. Plasmodium vivax aldolase-specific monoclonal antibodies and its application in clinical diagnosis of malaria infections in China. Malar J 2013; 12: 199.   DOI
4 Meier B, Dobeli H, Certa U. Stage-specific expression of aldolase isoenzymes in the rodent malaria parasite Plasmodium berghei. Mol Biochem Parasitol 1992; 52: 15-27.   DOI
5 Harani MS, Beg MA, Khaleeq L, Adil SN, Kakepoto GN, Khurshid M. Role of ICT malaria immunochromatographic test for rapid diagnosis of malaria. J Pak Med Assoc 2006; 56: 167-171.
6 Dzakah EE, Kang K, Ni C, Tang S, Wang J, Wang J. Comparative performance of aldolase and lactate dehydrogenase rapid diagnostic tests in Plasmodium vivax detection. Malar J 2014; 13: 272.   DOI
7 Kohler G, Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 1975; 256: 495-497.   DOI
8 Galfre G, Milstein C. Preparation of monoclonal antibodies: strategies and procedures. Methods Enzymol 1981; 73: 3-46.
9 Armbruster DA, Pry T. Limit of blank, limit of detection and limit of quantitation. Clin Biochem Rev 2008; 29 (suppl): 49-52.
10 Mendis K, Sina BJ, Marchesini P, Carter R. The neglected burden of Plasmodium vivax malaria. Am J Trop Med Hyg 2001; 64: 97-106.   DOI
11 Beeson JG, Chu CS, Richards JS, Nosten F, Fowkes FJ. Plasmodium vivax malaria: challenges in diagnosis, treatment and elimination. Pediatr Infect Dis J 2015; 34: 529-531.   DOI
12 Moody A. Rapid diagnostic tests for malaria parasites. Clin Microbiol Rev 2002; 15: 66-78.   DOI
13 Heutmekers M, Gillet P, Maltha J, Scheirlinck A, Cnops L, Bottieau E, Van Esbroeck M, Jacobs J. Evaluation of the rapid diagnostic test CareStart pLDH Malaria (Pf-pLDH/pan-pLDH) for the diagnosis of malaria in a reference setting. Malar J 2012; 11: 204.   DOI
14 World Health Organization. Malaria Rapid Diagnostic Test Performance: result of WHO product testing of malaria RDTs Round 3 (2010-2011). [Internet]. 2011. Available from: http://www.who.int/malaria/publications/atoz/9789241502566/en/.
15 Abba K, Deeks JJ, Olliaro P, Naing CM, Jackson SM, Takwoingi Y, Donegan S, Garner P. Rapid diagnostic tests for diagnosing uncomplicated P. falciparum malaria in endemic countries. Cochrane Database Syst Rev 2011: CD008122.
16 Ouattara A, Doumbo S, Saye R, Beavogui AH, Traore B, Djimde A, Niangaly A, Kayentao K, Diallo M, Doumbo OK, Thera MA. Use of a pLDH-based dipstick in the diagnostic and therapeutic follow-up of malaria patients in Mali. Malar J 2011; 10: 345.   DOI
17 Bashir IM, Otsyula N, Awinda G, Spring M, Schneider P, Waitumbi JN. Comparison of PfHRP-2/pLDH ELISA, qPCR and microscopy for the detection of Plasmodium events and prediction of sick visits during a malaria vaccine study. PLoS One 2013; 8: e56828.   DOI
18 Kim JH, Lee J, Sohn HJ, Song HO, Kim JY, Lee WJ, Park H, Shin HJ. Production of monoclonal antibodies for Plasmodium vivax lactate dehydrogenase and patient sera screening using sandwich ELISA. Parasitol Res 2012; 111: 1645-1650.   DOI
19 Centers for Disease Control and Prevention. Malaria. [Internet]. 2017. Available from: https://www.cdc.gov/malaria/.
20 Bartoloni A, Zammarchi L. Clinical aspects of uncomplicated and severe malaria. Mediterr J Hematol Infect Dis 2012; 4: e2012026.   DOI