• Journal of Embryo Transfer
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• v.23 no.3
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• pp.133-139
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• 2008

DNA methylation is one of the reasons for poor survival of clone animals. The OCT-4 gene is essential for maintaining pluripotency of embryonic stem (ES) cells and early embryos. We previously reported that the 5'-promoter region of Oct-4 gene was a target of DNA methylation and the methylation status was changed variously during embryonic development in bovine. The study conducted to examine the expression and methylation pattern of tissue-dependent differentially methylated region (T-DMR) of Oct-4 gene in bovine somatic cell nuclear transfer (SCNT) and in vitro fertilization (IVF) blastocysts. The Oct-4 gene expression was evaluated by RT-PCR and fluorescence immunocytochemistry. The methylation pattern of T-DMR was analyzed using restriction mapping and bisulfite sequencing methods. The Oct-4 transcripts were highly expressed in IVF, while they were not expressed in SCNT. The Oct-4 protein was not detected or expressed at very low level in SCNT, the intensity of Oct-4 protein, however, was strong in IVF. On the other hand, the T-DMR of Oct-4 gene was hypermethylated in SCNT compared to that of IVF. These results suggested that expression and the failure of demethylation of Oct-4 gene was closely associated with incomplete development of SCNT embryos.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.27 no.1
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• pp.31-38
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• 2015

Geothermal heat pump (GHP) systems have become an efficient alternative to conventional cooling and heating methods due to their higher energy using efficiency. These systems use the ground as a heat source in heating mode operation and a heat sink in cooling mode operation. The aim of this study is to evaluate the heating performance of the GHP system for a residential building ($420m^2$) in Ulaanbaatar, Mongolia. In order to demonstrate the feasibility of a sustainable performance of this system, we installed the water-to-water geothermal heat pump with ten vertical ground heat exchangers and measured operation parameters from October 19, 2013 to March 26, 2014. The results showed that the entering source temperature of brine from the ground heat exchangers was in a range of the design target temperature of $-10^{\circ}C$ for heating. For total values of the representative results, the ground heat exchangers extracted heat of 53.51 MWh from the ground. In addition, the GHP system supplied heat of 83.55 MWh to the building and consumed power of 30.27 MWh. Consequently, the average heating seasonal performance factor ($SPF_h$) of the overall system was evaluated to be 2.76 during the measurement period of the heating season.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.38 no.2
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• pp.160-168
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• 2001

Change detection using difference picture has been used to detect the location of moving targets and to track them. This method needs the assumption of static camera, and the global motion compensation is required in case of a moving camera. This paper suggests a method for finding a minimum bounding rectangles(MBR) of moving targets in the image sequences using moving region detection, especially with a moving camera. If the global motion parameter is inaccurately estimated, the estimated locations of targets will be accurate either To alleviate this problem, we introduce the concept of the confidence measure and achieve more accurate estimation of global motion. Experimental results show that the proposed method successfully removes background region and extracts MBRs of the targets. Even with a moving camera, the new global motion estimation algorithm performs more precise]y and it reduces the background compensation errors of change detection.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.12 no.2
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• pp.800-816
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• 2018

In recent years, video surveillance research has been able to recognize various behaviors of pedestrians and analyze the overall situation of objects by combining image analysis technology and deep learning method. Human Activity Recognition (HAR), which is important issue in video surveillance research, is a field to detect abnormal behavior of pedestrians in CCTV environment. In order to recognize human behavior, it is necessary to detect the human in the image and to estimate the pose from the detected human. In this paper, we propose a novel approach for 2D Human Pose Estimation based on object detection using RGB-D information. By adding depth information to the RGB information that has some limitation in detecting object due to lack of topological information, we can improve the detecting accuracy. Subsequently, the rescaled region of the detected object is applied to ConVol.utional Pose Machines (CPM) which is a sequential prediction structure based on ConVol.utional Neural Network. We utilize CPM to generate belief maps to predict the positions of keypoint representing human body parts and to estimate human pose by detecting 14 key body points. From the experimental results, we can prove that the proposed method detects target objects robustly in occlusion. It is also possible to perform 2D human pose estimation by providing an accurately detected region as an input of the CPM. As for the future work, we will estimate the 3D human pose by mapping the 2D coordinate information on the body part onto the 3D space. Consequently, we can provide useful human behavior information in the research of HAR.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.50 no.4
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• pp.567-582
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• 2014

Coastal fisheries in Korean waters have highly complexity with a variety of fishing gears, and scale of those fisheries is smaller than that of offshore fisheries. As a result, important spawning and nursery grounds for many species of fish has been destroyed. The pragmatic ecosystem-based approach was developed for the assessment of fisheries resources in Korean waters by Zhang et al. (2009; 2010). As for the species risk index (SRI), common squid caught by coastal gillnet in the Uljin region had the highest risk. As for the fisheries risk index (FRI), coastal gillnet in the Uljin coastal waters had the highest risk. For the common squid which had the highest SRI, resources management strategies must be established such as catch prohibition of length and period with TAC. For the coastal gillnet in the Uljin region which had the highest FRI, it is judged to need management plans for conserving biodiversity as reducing the catch of non-target species and discards. Also to protect existing habitat, illegal fishery should be prohibited, and fishing gears should be designed in the environmental-friendly way considering when fishing gears lost.

• Journal of the Korean Society of Radiology
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• v.7 no.3
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• pp.245-249
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• 2013

Neutron sources from photonuclear reaction with 46-MeV electron linear accelerator at Research Reactor Institute, Kyoto University used for resonance energy measurement of natural tantalum. BGO($Bi_4Ge_3O_{12}$) scintillation detectors used for measurement of the prompt gamma ray from the natural tantalum sample. The BGO spectrometer was composed geometrically as total energy absorption detector. The electric signal from the spectrometer was analyzed for TOF(Time-of-Flight) spectrum which is used identification of neutron capture resonance energy. In this study, the neutron energy region is from 1 to 200 eV, because of strong X-ray effect produced photonuclear reaction in Ta target, the measurement was performed to below 1 keV energy region. The resonance energy was compared with the evaluated values(ENDF/B-VI, Mughabghab). All of the resonances from 4.28 ~ 200 eV were seen in the present measurement except 144.3 eV resonance.

• Journal of Cadastre & Land InformatiX
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• v.46 no.2
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• pp.137-153
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• 2016

The price of agricultural products has changed from year to year, the m ajor c ause o f price fluctuation is the imbalance of supply and demand. Materials which are mainly used in korean cabbage production volume is the forecast model, using the cadastral result, slope calculation is impossible to achieved. For this reason, this implies the drastic decrease of prices and the prediction of supply and demand of field crops that is cultivated in a highland slope area, this situation is being repeated. Therefore, the target area of this research is the slopes of high land, by using 2D and 3D Lidar data for the analysis of the cultivated area. Experiment was carried out in the same area to compare the data differences. The rate of change in the area of slope is quantitatively increasing presented by the regression model. An alternative methodology that can improve the reliability of the calculated slope area using 2D is through cadastral map.

• Journal of Applied Biological Chemistry
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• v.53 no.4
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• pp.189-194
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• 2010

We confirmed the hypo-osmotic shock strengths and duration, different type of vectors, and subcelluar localization to identify the optimum analysis condition of plant aquaporin activity in Xenopus ooctye using Arabidopsis thaliana AtPIP2-1 gene. Six minutes and 1/5ND buffer hypoosmotic shock treatment was the best condition to show the maximum swelling of Xenopus oocytes where AtPIP2-1 was expressed using pcDNA3.1 vector. AtPIP2-1 protein was expressed more efficiently in pGEMHE vector which has 5' and 3' UTR (untranslation region) of Xenopus ${\beta}$-GLOBIN gene in multiple cloning site than in pcDNA3.1 vector. Also green fluorescence of GFP fused to AtPIP2-1 was detected onto oocyte plasmamembrane where is the proper subcellular localization target of AtPIP2-1.

• Asian Journal of Atmospheric Environment
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• v.9 no.4
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• pp.259-271
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• 2015

This study conducted analyses on biogenic volatile organic compounds (BVOC) emission sources contributing to urban ozone ($O_3$) concentration in Osaka Prefecture, Japan in summer 2010 by using the Weather Research and Forecasting model (WRF) version 3.5.1 and the Community Multiscale Air Quality model (CMAQ) version 5.0.1. This prefecture is characterized by highly urbanized area with small forest area. The contributions of source regions surrounding Osaka were estimated by comparing the baseline case and zero-out cases for BVOC emissions from each source region. The zero-out emission runs showed that the BVOC emissions substantially contributed to urban $O_3$ concentration in Osaka (10.3 ppb: 15.9% of mean daily maximum 1-h $O_3$ concentration) with day-by-day variations of contributing source regions, which were qualitatively explained by backward trajectory analyses. Although $O_3$ concentrations were especially high on 23 July and 2 August 2010, the contribution of BVOC on 23 July (35.4 ppb: 25.6% of daily maximum $O_3$) was much larger than that on 2 August (20.9 ppb: 14.2% of daily maximum $O_3$). To investigate this difference, additional zero-out cases for anthropogenic VOC (AVOC) emissions from Osaka and for VOC emissions on the target days were performed. On 23 July, the urban $O_3$ concentration in Osaka was dominantly increased by the transport from the northwestern region outside Osaka with large contribution of $O_3$ that was produced through BVOC reactions by the day before and was retained over the nocturnal boundary layer. On 2 August, the concentration was dominantly increased by the local photochemical production inside Osaka under weak wind condition with the particularly large contribution of AVOC emitted from Osaka on the day.

• The Journal of the Korea Contents Association
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• v.10 no.10
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• pp.398-407
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• 2010

The purpose of this study is to explain the neighborhood region "gimpo northwest area" connection development make use of a core tourist area "kanghwado" of visitor behavior characteristics. this is a foundation data for research of kanghwado behavior characteristics. For this research, the description of the current study were as follows. First kanghwado revisitors of high potentialities visit gimpo northwest area. so its demanded same or different attraction development and make an target marketing from kanghwado revisitors. Second the gimpo northwest keep the accent on development history and experience education of housewives families. Third publicity board parking area and infrastructure is demanded on the 48th street. Forth Han river and Imjin river meeting point gimpo north area, each other kanghwado gimpo west put opposite Yeomha river there can see point the west sea. therefore we need waterside attraction.