• Animal Bioscience
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• v.37 no.3
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• pp.509-521
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• 2024

Objective: It was shown that microRNAs (miRNAs) play an important role in milk protein synthesis. However, the post-transcriptional regulation of casein expression by exogenous miRNA (xeno-miRNAs) in ruminants remains unclear. This study explores the regulatory roles of alfalfa xeno-miR162 on casein synthesis in bovine mammary epithelial cells (bMECs). Methods: The effects of alfalfa xenomiR-162 and G protein subunit gamma 11 (GNG11) on proliferation and milk protein metabolism of bMECs were detected by 5-Ethynyl-2'-Deoxyuridine (EdU) staining, flow cytometry, cell counting kit-8 (CCK-8), enzyme-linked immunosorbent assay, quantitative real-time polymerase chain reaction (qRT-PCR), and Western blot. Dual-luciferase reporter assay was used to verify the targeting relationship between GNG11 and xenomiR-162. Results: Results showed that over-expression of xenomiR-162 inhibited cell proliferation but promoted apoptosis, which also up-regulated the expression of several casein coding genes, including CSN1S1, CSN1S2, and CSN3, while decreasing the expression of CSN2. Furthermore, the targeting relationship between GNG11 and xenomiR-162 was determined, and it was confirmed that GNG11 silencing also inhibited cell proliferation but promoted apoptosis and reduced the expression of casein coding genes and genes related to the mammalian target of rapamycin (mTOR) pathway. Conclusion: Alfalfa xenomiR-162 appears to regulate bMECs proliferation and milk protein synthesis via GNG11 in the mTOR pathway, suggesting that this xeno-miRNA could be harnessed to modulate CSN3 expression in dairy cows, and increase κ-casein contents in milk.

• Korean Journal of Applied Biomechanics
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• v.26 no.2
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• pp.197-204
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• 2016

Objective: The purpose of this study was to analyze the kinematic factors of motion during air pistol shooting. Method: This study aimed to investigate changes in forces during movement and determine the factors that affect changes in force during the first, middle, and last periods of shooting an air pistol. Two ground reaction force systems (force platform), SCATT (a shooting training system), and EMG (electromyogram) to measure the action potentials in the muscles of the upper body were used in this study. Four university air pistol players (age: 19.75 years, height: 175.50 cm, body mass: $69.55{\pm}11.50kg$, career length: $6.25{\pm}6years$) who are training to progress to a higher rank were enrolled. Results: In terms of the actual shooting results, the mean score in the middle section was $42.48{\pm}1.74$ points, higher than those in the first and the last periods when using SCATT. The gunpoint moved 13.48 mm more vertically than horizontally in the target trajectory. With respect to action potentials of muscles measured using EMG, the highest action potentials during the aiming-shooting segments, in order higher to lower, were seen in the trapezius (intermediate region), trapezius (superior region), deltoid (lateral), and triceps brachii (long head). The action potentials of biceps brachii and brachioradialis turned out to be high during grasping motion, which is a preparatory stage. During the final segment, muscle fatigue appeared in the deltoid (lateral), biceps brachii (long head), brachioradialis, and trapezius (intermediate region). In terms of the ground reaction force, during the first period of shooting, there was a major change in the overall direction (left-right $F_x$, forward-backward $F_y$, vertical $F_z$) of the center of the mass. Conclusion: The development and application of a training program focusing on muscle groups with higher muscle fatigue is required for players to progress to a higher rank. Furthermore, players can improve their records in the first period if they take part in a game after warming up sufficiently before shooting in order to heighten muscle action potentials, and are expected to maintain a consistent shooting motion continuously by restoring psychological stability.

• Journal of Korea Multimedia Society
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• v.17 no.2
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• pp.132-142
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• 2014

Stereo camera is drawing attention as an essential sensor for future intelligence robot system since it has the advantage of acquiring not only distance but also other additive information for an object. However, it cannot match correlated point on target image for low textured region or periodic patterned region such as wall of building or room. In this paper, we propose a stereo matching technique that increase the matching performance by fusing belief propagation stereo matching algorithm and local distance measurements of 2D-laser range finder in order to overcome this kind of limitation. The proposed technique adds laser measurements by referring quad-tree based segment information on to the local-evidence of belief propagation stereo matching algorithm, and calculates compatibility function by reflecting over-segmented information. Experimental results of the proposed method using simulation and real test images show that the distance information for some low textured region can be acquired and the discontinuity of depth information is preserved by using segmentation information.

• Journal of the Korean Magnetic Resonance Society
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• v.18 no.1
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• pp.30-35
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• 2014

The transcription factor CP2 regulates various biological systems at diverse tissues and cells. However, none of the four CP2 isoforms has been solved in structure yet. In particular, two different regions of the CP2b isoform have been characterized to interact with the PIAS1 in nucleus to regulate the ${\alpha}$-globin gene expression. Among them, in this study, the region encompassing residues 251-309 of CP2b was prepared as a recombinant protein and its solution structure was characterized by NMR spectroscopy. The results indicated that the CP2b(251-309) fold belongs to typical IDRs (intrinsically disordered regions), likely to facilitate promiscuous interactions with various target proteins. Unfortunately, however, its interaction with the N-terminal domain of PIAS1 (residues 1-70), which has been identified as one of the CP2b-binding sites, was not observed in the NMR-based titration experiments. Therefore, it could be postulated that the 251-309 region of CP2b would not contact with the PIAS1(1-70), but alternatively interact with another CP2b-binding region that encompasses residues 400-651 of PIAS1.

• The Journal of Korean Physical Therapy
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• v.6 no.1
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• pp.61-74
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• 1994

This study was carried out to determine effects of electrical stimulation on the soleus, target muscle of the sciatic newt, of white rat normal muscles. The biometric, histochemical, ultrastructural observations were made. The following results were obtained. A daily electrical stimulation of the skeletal muscle of the normally-functioning rat caused an increase of girth and weight of the muscle fibers for 2 weeks. No noticeable change was observed afterwards. More specifically, the density of volume of the red muscle fiber increased. whereas the density of the white muscle fiber decreased. The electrical stimulation group(experimental group) showed hypertrophy of the muscle fibers and narrowing of the space between perimysium and endomysium. Normally, glycogen granules are accumulated regardless of classification of muscle fibers. In addition, the NADH-TR reaction results were in agreement with the biometric findings, in that the red muscle fibers significantly increased. The ultrastructural observations revealed that mitochondria was formed in the red muscle fiber parallel to the muscle fibers of normal muscle, while mitochondria was observed in the sarcomere region of the white muscle fiber. However, activation of mitochondria took place in the sarcolemma region of the muscle fiber, and generation of mitochondria was observed in the sarcomere region of the white muscle fiber.

• Journal of Microbiology and Biotechnology
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• v.16 no.7
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• pp.1104-1110
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• 2006

Recombinant antibody-toxin is a bifunctional protein that binds and kills a target cell expressing a specific antigen on the surface of the cell, and its structure is chimeric, in which a toxin is fused to an antigen-binding domain such as scFv or Fab. Divalent antibody-toxin molecules showed higher cytotoxicities against cancer cell lines than monovalent molecules. However, the yields of the divalent molecules were very low. In this study, we introduced the CH2, CH3, or CH2-CH3 (=Fc) domain of antibody in the middle of the Fab-toxin between the hinge region of human IgG1 and the toxin domain to increase the yield. The covalently bonded dimer could be formed by three disulfide bridges from cysteine residues in the hinge region. The molecule with the CH3 domain showed about 3-fold higher dimerization yield than previously constructed Fab-toxin molecules, while maintaining the cytotoxic activity comparable to that of scFv-toxin. However, the introduction of CH2 or Fc domain to the same position showed little effect on the dimerization yield. We also observed that the introduction of the CH3 region made it possible to form noncovalently associated dimer molecules.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.5
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• pp.697-704
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• 2018

Since the face in image content corresponds to individual information that can distinguish a specific person from other people, it is important to accurately detect faces not hidden in an image. In this paper, we propose a method to accurately detect a face from input images using a deep learning algorithm, which is one of the machine learning methods. In the proposed method, image input via the red-green-blue (RGB) color model is first changed to the luminance-chroma: blue-chroma: red-chroma ($YC_bC_r$) color model; then, other regions are removed using the learned skin color model, and only the skin regions are segmented. A CNN model-based deep learning algorithm is then applied to robustly detect only the face region from the input image. Experimental results show that the proposed method more efficiently segments facial regions from input images. The proposed face area-detection method is expected to be useful in practical applications related to multimedia and shape recognition.

• Journal of The Korean Astronomical Society
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• v.47 no.6
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• pp.319-325
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• 2014

We map two molecular clouds located in the exact anticenter region emitting in the (J = 1-0) transition of $^{12}CO$ and $^{13}CO$ using the 3-mm SIS mixer receiver on the 14-m radio telescope at Taeduk Radio Astronomy Observatory. The target clouds with anomalous velocities of $V_{LSR}{\sim}-20km\;s^{-1}$ are distinguished from other clouds in this direction. In addition, they are located in the interarm region between the Orion Arm and the Perseus Arm. Sizes of the clouds are estimated to be about 8.6 and 10.8 pc, respectively. The total mass is estimated to be about $4{\times}10^3$ $M_{\odot}$ using CO luminosity of the clouds. Several cores are detected, but no sign of star formation is found according to the IRAS point sources. Their larger linewidths, anomalous velocities, and their location at the interarm region make these clouds more distinguished, though their physical properties are similar to the dark clouds in the solar neighborhood in terms of mass and size.

• Biomedical Science Letters
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• v.23 no.3
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• pp.272-276
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• 2017

HOX genes are transcription factors that play important roles in body patterning and cell fate specification during normal development. In previous study, we found aberrant overexpression of HOXB5 in breast cancer tissues and cell lines, and demonstrated that HOXB5 is important in regulation of cell proliferation, tamoxifen resistance, and invasiveness through the epithelial-mesenchymal transition (EMT). Although the relationship between HOXB5 and phenotypic changes in MCF7 breast cancer cells has been studied, the molecular function of HOXB5 as a transcription factor remains unclear. IL-6 has been reported to be involved in not only inflammation but also cancer progression, which is characterized by the increase of growth speed and invasiveness of tumor cells. In this study, we selected Interleukin-6 (IL-6) as HOXB5 putative downstream target gene and discovered that HOXB5 transcriptionally up-regulated the expression of IL-6 in HOXB5 overexpressing MCF7 cells. The upstream region (~1.2 kb) of IL-6 promoter turned out to contain several putative HOX consensus binding sites. Chromatin immunoprecipitation assay confirmed that HOXB5 directly binds to the promoter region of IL-6 and positively regulated the expression of IL-6. These data all together, indicate that HOXB5 promotes IL-6 transcription by actively binding to the putative binding sites located in the upstream region of IL-6, which enable to increase its promoter activity in MCF7 breast cancer cells.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.18 no.1
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• pp.135-151
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• 2015

DMZ is considered as an ecologically sensitive landscape and one of the highest biodiversity regions in the Republic of Korea. There have been, albeit the significant value, increased interests in developing this region for a variety of purposes including tourism and commemorative events. As this region has been already facing a range of problems derived from previous development, natural disaster and invasive species, the necessity for active management of ecological health within this region has been increased, which weighs the importance of executing ecological restoration. The objective of this study was to develop evaluation indices as an effective management means of properly evaluating ecological restoration and sustainably maintaining the restored conditions on a long-term scale. Through literature review existing evaluation indices related to restoration were collected, and then the most suitable indices were selected based upon two interviews and one questionnaire survey targeting experts in the relevant field to ecological restoration. They were categorized by two major division and their subclasses (Ecological base - vegetation structure & composition, habitat characteristics, soil environment; landscape ecology - connectivity, landscape patch, boundary & surrounding) and 40 indices. These indices were considered helpful to comprehensively evaluate ecological restoration on degraded environments within ecologically sensitive areas, and sustainably manage target areas by employing a long-term monitoring approach. As this result played a meaningful role in providing the fundamentals of evaluating ecological restoration, it should develop a suitable evaluation system through further research.