• Bulletin of the Korean Chemical Society
• /
• v.34 no.5
• /
• pp.1467-1472
• /
• 2013

Metabolomics is a field that studies systematic dynamics and secretion of metabolites from cells to understand biological pathways based on metabolite changes. The metabolic profiling of intact human colorectal tissues was performed using high-resolution magic angle spinning (HR-MAS) NMR spectroscopy, which was unnecessary to extract metabolites from tissues. We used two different groups of samples, which were defined as normal and cancer, from 9 patients with colorectal cancer and investigated the samples in NMR experiments with a water suppression pulse sequence. We applied target profiling and multivariative statistical analysis to the analyzed 1D NMR spectra to identify the metabolites and discriminate between normal and cancer tissues. Cancer tissue showed higher levels of arginine, betaine, glutamate, lysine, taurine and lower levels of glutamine, hypoxanthine, isoleucine, lactate, methionine, pyruvate, tyrosine relative to normal tissue. In the OPLS-DA (orthogonal partial least square discriminant analysis), the score plot showed good separation between the normal and cancer groups. These results suggest that metabolic profiling of colorectal cancer could provide new biomarkers.

• Molecules and Cells
• /
• v.38 no.6
• /
• pp.475-481
• /
• 2015

Programmable nucleases, which include zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and RNA-guided engineered nucleases (RGENs) repurposed from the type II clustered, regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) system are now widely used for genome editing in higher eukaryotic cells and whole organisms, revolutionising almost every discipline in biological research, medicine, and biotechnology. All of these nucleases, however, induce off-target mutations at sites homologous in sequence with on-target sites, limiting their utility in many applications including gene or cell therapy. In this review, we compare methods for detecting nuclease off-target mutations. We also review methods for profiling genome-wide off-target effects and discuss how to reduce or avoid off-target mutations.

• Journal of the Korean Magnetic Resonance Society
• /
• v.20 no.1
• /
• pp.13-16
• /
• 2016

Various experiments using zebrafish have been highlighted recently in the scientific community. Because it is possible to conduct practical experiment from various neurological research to area of genetic study or toxicity experiment. However, gender difference effects are nearly not considered. If the gender differences of zebrafish are considered it is possible to obtain more accurate data. In this study, zebrafish which have different genders were compared each other with NMR-based metabolomics. The extracts of male and female zebrafish were measured by 600 MHz NMR spectrometer. Statistical analysis and target profiling were conducted. As a result, muscle related metabolites were observed in male zebrafish and nerve related metabolites were observed in female zebrafish.

• Proceedings of the SCSK Conference
• /
• 2003.09b
• /
• pp.218-218
• /
• 2003

Recently we showed the prototype portable device for the determination of human skin moisture by using near infrared spectroscopy. In order to optimize the acquiring condition of NIR spectrum of skin and control the target information of water depending the site such as epidermis and dermis, skin depth profiling was investigated changing the distance between illuminations and receiving of radiation in the terminal of fiber probe. The colleted light information could be controlled by changing the distance of the fiber optic probes. It was confirmed that the longer distance we used, the deeper site from the skin surface we could get information from in this study. Four kinds of probes with distances such as 0.03 mm, 0.1 mm, 0.5 mm, and 1.0 mm were used. In addition, the gap size from 0.3 mm to 3.0 mm was studied to control the intensity of water absorbance effectively and to avoid saturation of water absorption. We also investigated the reference materials depending the reflectance ratio for water absorption not to be saturated because of the strong absorptivity of water. Furthermore, spectroscopic information regarding free water and bound water around 1850 nm was investigated by using the different distance of fiber optic probes. This study would be great help to control the spectroscopic information of water to be measured depending the site where water exists.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2014.05a
• /
• pp.617-618
• /
• 2014

본 논문에서는 보다 정확하고 효과적인 평판분석을 위하여 서비스 산업별 타겟으로 하는 수집사이트를 프로파일링 하는 방법을 제안한다. 먼저 각 서비스에 특화된 타겟 사이트를 추출하고 등록하고 각 서비스에 관련한 정보 및 의견 공유 게시판과 지식인 추천/질문 등 지식 공유 사이트를 추출한다. 또한 업종별 주요 사이트를 선택하고 등록하여 유효 데이터 수집한다. 이를 통해 실시간 수집 데이터의 활용 기술을 이용하여 수집원 프로파일링을 통한 미디어별 수집 주기 산정하고 수집 엔진의 유연한 확장성을 활용한 실시간 수집 제반 기술 확대할 수 있다. 또한 지속적인 수집원 변경관리를 수행한다. 즉, 신규 생성, 변경, 삭제되는 사이트에 대한 변경관리를 수행하고 지속적인 수집량 모니터링을 통한 수집여부를 점검하며 수집 필터링 규칙에 대한 튜닝으로 데이터 품질 확보하도록 한다.

• Biomolecules & Therapeutics
• /
• v.29 no.2
• /
• pp.135-143
• /
• 2021

Drug addiction influences most communities directly or indirectly. Increasing studies have reported the relationship between circadian-related genes and drug addiction. Per2 disrupted mice exhibited more vulnerable behavioral responses against some drugs including methamphetamine (METH). However, its roles and mechanisms are still not clear. Transcriptional profiling analysis in Per2 knockout (KO) mice may provide a valuable tool to identify potential genetic involvement and pathways in enhanced behavioral responses against drugs. To explore the potential genetic involvement, we examined common differentially expressed genes (DEGs) in the striatum of drug naïve Per2 KO/wild-type (WT) mice, and before/after METH treatment in Per2 KO mice, but not in WT mice. We selected 9 common DEGs (Ncald, Cpa6, Pklr, Ttc29, Cbr2, Egr2, Prg4, Lcn2, and Camsap2) based on literature research. Among the common DEGs, Ncald, Cpa6, Pklr, and Ttc29 showed higher expression levels in drug naïve Per2 KO mice than in WT mice, while they were downregulated in Per2 KO mice after METH treatment. In contrast, Cbr2, Egr2, Prg4, Lcn2, and Camsap2 exhibited lower expression levels in drug naïve Per2 KO mice than in WT mice, while they were upregulated after METH treatment in Per2 KO mice. qRT-PCR analyses validated the expression patterns of 9 target genes before/after METH treatment in Per2 KO and WT mice. Although further research is required to deeply understand the relationship and roles of the 9 target genes in drug addiction, the findings from the present study indicate that the target genes might play important roles in drug addiction.

• Journal of Distribution Science
• /
• v.20 no.6
• /
• pp.1-10
• /
• 2022

Purpose: This research proposes a new modified Recency-Frequency-Monetary (RFM) model by extending the model with spatial analysis for supporting decision-makers in discovering the promotional target market. Research design, data and methodology: This quantitative research utilizes data-mining techniques and the RFM model to cluster a university's provider schools. The RFM model was modified by adapting its variables to the university's marketing context and adding a district's potential (D) variable based on heatmap analysis using Geographic Information System (GIS) and K-means clustering. The K-prototype algorithm and the Elbow method were applied to find provider school clusters using the proposed RFM-D model. After profiling the clusters, the target segment was assigned. The model was validated using empirical data from an Indonesian university, and its performance was compared to the Customer Lifetime Value (CLV)-based RFM utilizing accuracy, precision, recall, and F1-score metrics. Results: This research identified five clusters. The target segment was chosen from the highest-value and high-value clusters that comprised 17.80% of provider schools but can contribute 75.77% of students. Conclusions: The proposed model recommended more targeted schools in higher-potential districts and predicted the target segment with 0.99 accuracies, outperforming the CLV-based model. The empirical findings help university management determine the promotion location and allocate resources for promotional information distribution and student recruitment.

• Journal of Microbiology and Biotechnology
• /
• v.23 no.7
• /
• pp.923-931
• /
• 2013

Rapamycin, known as an inhibitor of Target of Rapamycin (TOR), is an immunosuppressant drug used to prevent rejection in organ transplantation. Despite the close association of the TOR signaling cascade with various scopes of metabolism, it has not yet been thoroughly investigated at the metabolome level. In our current study, we applied mass spectrometric analysis for profiling primary metabolism in order to capture the responsive dynamics of the Chlamydomonas metabolome to the inhibition of TOR by rapamycin. Accordingly, we identified the impact of the rapamycin treatment at the level of metabolomic phenotypes that were clearly distinguished by multivariate statistical analysis. Pathway analysis pinpointed that inactivation of the TCA cycle was accompanied by the inhibition of cellular growth. Relative to the constant suppression of the TCA cycle, most amino acids were significantly increased in a time-dependent manner by longer exposure to rapamycin treatment, after an initial down-regulation at the early stage of exposure. Finally, we explored the isolation of the responsive metabolic factors into the rapamycin treatment and the culture duration, respectively.

• Proceedings of the Korea Information Processing Society Conference
• /
• 2009.11a
• /
• pp.13-14
• /
• 2009

Intermediate Representation(IR)은 컴파일러가 Target code 를 생성하기까지의 중간 단계에서 프로그램을 표현하는 수단이다. 본 논문에서는 컴파일러의 IR을 직접 Simulation 해서 각종 profiling information을 수집하여 프로그램의 성능 향상에 도움을 줄 수 있는 IR Simulator를 제안하고자 한다. 더 나아가 IR Simulator를 위한 Data structure와 Interface는 Target Simulation에도 재사용이 가능하도록 고안되었기 때문에 재겨냥성 컴파일러의 Target Simulator를 자동으로 생성하는 연구를 진행하고 있다.

• Molecules and Cells
• /
• v.37 no.9
• /
• pp.691-698
• /
• 2014

SCYL1-BP1 is thought to function in the p53 pathway through Mdm2 and hPirh2, and mutations in SCYL1-BP1 are associated with premature aging syndromes such as Geroderma Osteodysplasticum; however, these mechanisms are unclear. Here, we report significant alterations in miRNA expression levels when SCYL1-BP1 expression was inhibited by RNA interference in HEK293T cells. We functionally characterized the effects of potential kernel miRNA-target genes by miRNA-target network and protein-protein interaction network analysis. Importantly, we showed the diminished SCYL1-BP1 dramatically reduced the expression levels of EEA1, BMPR2 and BRCA2 in HEK293T cells. Thus, we infer that SCYL1-BP1 plays a critical function in HEK293T cell development and directly regulates miRNA-target genes, including, but not limited to, EEA1, BMPR2, and BRCA2, suggesting a new strategy for investigating the molecular mechanism of SCYL1-BP1.