• Title/Summary/Keyword: Target indicator

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Hybridization by an Electrical Force and Electrochemical Genome Detection Using an Indicator-free DNA on a Microelectrode-array DNA Chip

  • Choi, Yong-Sung;Lee, Kyung-Sup;Park, Dae-Hee
    • Bulletin of the Korean Chemical Society
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    • v.26 no.3
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    • pp.379-383
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    • 2005
  • This research aims to develop DNA chip array without an indicator. We fabricated microelectrode array by photolithography technology. Several DNA probes were immobilized on an electrode. Then, indicator-free target DNA was hybridized by an electrical force and measured electrochemically. Cyclic-voltammograms (CVs) showed a difference between DNA probe and mismatched DNA in an anodic peak. Immobilization of probe DNA and hybridization of target DNA could be confirmed by fluorescent. This indicator-free DNA chip microarray resulted in the sequence-specific detection of the target DNA quantitatively ranging from $10^{-18}\;M\;to\;10^{-5}$ M in the buffer solution. This indicator-free DNA chip resulted in a sequence-specific detection of the target DNA.

Indicator-free DNA Chip Array Using an Electrochemical System

  • Park, Yong-Sung;Kwon, Young-Soo;Park, Dae-Hee
    • KIEE International Transactions on Electrophysics and Applications
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    • v.4C no.4
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    • pp.133-136
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    • 2004
  • This research aims to develop a DNA chip array without an indicator. We fabricated a microelectrode array through photolithography technology. Several DNA probes were immobilized on an electrode. Then, target DNA was hybridized and measured electrochemically. Cyclic-voltammograms (CVs) showed a difference between the DNA probe and mismatched DNA in an anodic peak. This indicator-free DNA chip resulted in a sequence-specific detection of the target DNA.

Development of New DNA Chip and Genome Detection Using an Indicator-free Target DNA (비수식화 DNA를 이용한 유전자 검출 및 새로운 DNA칩의 개발)

  • Park, Yong-Sung;Park, Dae-Hee;Kwon, Young-Soo;Tomoji Kawai
    • The Transactions of the Korean Institute of Electrical Engineers C
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    • v.52 no.8
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    • pp.365-370
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    • 2003
  • This research aims to develop an indicator-free DNA chip using micro-fabrication technology. At first, we fabricated a DNA microarray by lithography technology. Several probe DNAs consisting of thiol group at their 5-end were immobilized on the gold electrodes. Then indicator-free target DNA was hybridized by an electrical force and measured electrochemically in potassium ferricyanide solution. Redox peak of cyclic-voltammogram showed a difference between target DNA and mismatched DNA in an anodic peak current. Therefore, it is able to detect various genes electrochemically after immobilization of various probe DNAs and hybridization of indicator-free DNA on the electrodes simultaneously It suggested that this DNA chip could recognize the sequence specific genes.

Improvement of Biotope-Area-Ratio-Indicator and Appraisal System Applicable to Environmental Impact Assessment Projects (환경영향평가 대상사업에 적용가능한 생태면적률 지표 평가체계 개선)

  • Lee, Gwan-Gyu;Lee, Sang-Hyuk;Kim, Gyung-Ho;Lee, Jung-Hwan
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.14 no.5
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    • pp.113-125
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    • 2011
  • Since the government of Korea (Ministry of Environment, MOE) introduced the policy applying 'Biotope-Area-Ratio-Indicator (BARI)' to huge residential land developments which Environmental Impact Assessment (EIA) should be performed, MOE has come to have the necessity to apply the indicator concretely at the stage of Prior Environment Review System (PERS) and EIA in various types of large scale land development projects. This study was conducted with the aim of supporting the application of BARI and related decision making in various other types of EIA projects as well as residential development projects through remodeling the system to apply the indicator of the past. Through the analysis of the problems in applying the past BARI and experimental appraisals to 11 types of EIA projects, the results and implications as follows were drawn. First, it's possible to extend the range of applications of BARI, which has been applied to only residential land development project, to all kinds of projects with area-typed land use pattern out of environmental impact assessment target projects. Second, it's also possible to set a target value into which regional characteristics and differences among locational properties are reflected. In addition, it's come to be able to differentially apply the target value of BARI according to the condition of the existing site. Third, it's improved to be able to suggest a macroscopic target value at the stage of PERS and to set detailed target values in each detailed land use at the stage of EIA. The key point underlies inducing methodology to determine target values to secure more permeable land coverage ratio for detailed land use patterns at the stage of EIA by making it possible to calculate BARI of the present land cover condition of the EIA target projects.

Establishing Evaluation Indicator for Agricultural Utilization of Idle Farmlands and Field Application (유휴농지 농업적 활용 평가지표 설정 및 현장적용)

  • Kim, Kyoung-Chan;Park, Chang-Won;Cho, Seok-Ho;Choi, Jin-Gyu;Yoon, Seong-Soo;Son, Yong-Hoon
    • Journal of Korean Society of Rural Planning
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    • v.20 no.2
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    • pp.127-137
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    • 2014
  • Self-sufficiency rate of food in South Korea is almost at the lowest level among OECD countries, and the decrease tend of farmland is expected to be continued. In this situation, the government has been revised the target self-sufficiency rate of food, and carried forward various policies in order to achieve it. One of those policies is the restoration business of idle farmland which is planned to carry forward after 2015. This study set up indicators evaluating effective use of idle farmland, and tried to apply in the field before carrying forward restoration business. The result of this study may be summarized as follows. First of all, it reset an evaluation indicator that was based on the evaluation indicator developed in order to set application directions of idle farmland. Next, it selected 30 idle farmlands with reset evaluation indicator among 11,635 which were inspected nationwide in 2012. Before applying indicator, it measured the condition of recycling such as accessibility of farmland, condition of irrigation and drainage system, land state, and surroundings by field investigation. Then, it calculated composite score in each target area through applying indicators, and verified the indicator by comparing calculated result with the one which was decided from field investigation. Finally, it carried out field investigation, correct and upgrade some problems of the standard of score calculation that was found during applying previously set evaluation indicator to target area, and established the final standard of calculation for evaluation indicator.

Applicability of Water Quality Indicators as Target Pollutants for Total Maximum Daily Loads (수질오염총량관리를 위한 대상물질 적용성 분석)

  • Park, Jun Dae;Oh, Seung Young;Park, Jae Hong
    • Journal of Korean Society on Water Environment
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    • v.29 no.4
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    • pp.531-539
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    • 2013
  • In order to apply a water quality indicator as a certain target pollutant, the indicator should have the representability of an index for the management of Total Maximum Daily Loads (TMDLs). Scientific groundwork and treatment technologies also should be provided for the application of the indicator. This study analyzed the applicability of major water quality indicators for target pollutants. The results showed that four water quality indicators of TOC, T-N, SS & T-Coli can be considered as target pollutants besides existing BOD and T-P. These indicators can be applied with the preparations such as essential basic data, pollution load estimation framework as well as legal criteria setting when the need is raised. As the importance of TOC, which is considered as a replacement of BOD, is emphasized, technical and institutional terms are being prepared in order to determine TOC as another target pollutant in the near future. It could be considered to combine TOC with BOD for the time being during the $3^{rd}$ stage of TMDLs in view of the present conditions for the management of TOC. TOC can be the most effective index for the organic matter and help to be managed more systematically in the waterbodies where rivers and lakes are mutually connected.

Study on MMTI Signal Processing Algorithm and Analysis of the Performance for Periscope Detection in Airborne Radar (항공용 레이다를 이용한 잠망경 탐지 MMTI 신호처리 기법 연구 및 성능 분석)

  • Jung, Jae-Hoon;Lee, Jae-Min;Youn, Jae-Hyuk;Shin, Hee-Sub
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.28 no.8
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    • pp.661-669
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    • 2017
  • This paper describes an MMTI(Maritime Moving Target Indicator) for periscope detection in airborne radar. Firstly, we analyze the characteristics of sea clutter, sea targets. Secondly, we study the differences between GMTI(Ground Moving Target Indicator) and MMTI. This paper proposes an optimal MMTI operating environment and method. We also suggest a signal processing algorithm using STAP(Space-Time Adaptive Processing) for detecting small RCS target moving low speed. The detection probability for moving target with MDV(Minimum Detectable Velocity) is simulated under various RCS and multi-channel system. Finally, we analyze the major performance for range, velocity and azimuth accuracy.

A Study on the Indicator Development for the Target Technology Selection of Technology Assessment (기술영향평가 대상기술 선정 지표 개발에 관한 연구)

  • Han, Min-Kyu;Kang, Ji-Min
    • Journal of Technology Innovation
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    • v.19 no.1
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    • pp.55-78
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    • 2011
  • The social impact of science and technology is increasing, the meaning of technology assessment is not small in modern society, Specially, the technology assesment(TA) has been institutionalized and performed by Korean administration and its official results has reflected directly in the S&T policy. Therefore, the technology assesment is a device that prepare the negative effects of S&T and the social controversy in advance. To select the target technology of technology assessment will be done carefully in various perspective and concerned with the characteristics of technology assessment emphasizing social assessment, the institutional system of selecting one technology in each year and the contents and methods of evaluation that are determined in accordance with technology characteristics. However, the method of selecting target technology in TA is mostly peformed by qualitative discussion and vote rather than by reflecting various opinion and understanding TA until now. In this paper, we developed the indicator has to be considered to select target technology for TA by using factor analysis. Developed indicator is consist of five factor, such as the need for social consensus and the size of social/cultural impact etc., and we weight each factor by using Analytic Hierarchy Process(AHP). Futhermore, we show the example how to applicate directly these indicator and weights to select target technology and suggest institutional application in TA. Though using developed indicator in this paper, we expect to select appropriate technology for institutional TA and the application of TA results in society and public policy can be strengthened.

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A Study on the Development of the Advanced Energy Performance Indicator for the Manufacturing Companies (제조업체의 에너지성과지표 고도화에 관한 연구)

  • Rho, Kyung-Wan;Song, Myung-Ho
    • Journal of the Korean Solar Energy Society
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    • v.35 no.5
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    • pp.31-38
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    • 2015
  • It is important to improve the energy performance in the industrial sector, and therefore most of the manufacturing companies need the energy performance indicators to identify the target and to verify the energy savings. However, the conventional energy performance indicators such as the total energy consumption and the energy intensity are not proper to use. The reason is that they do not consider adequate relevant variables including productions in the boundary of the manufacturing companies. Therefore, the study provides the advanced energy performance indicator using by the linear regression model according to each energy source to manage the target and to verify the energy performance properly.

A Study On The Relationship Characteristics Between Inborn(Myers-Brigg's Type Indicator: MBTI) and Acquired(Personality Adaptation Type: PAT) Factors of Personality (성격의 선천적 요인(MBTI)과 후천적 요인(PAT)의 관계특성 연구)

  • Oh, Soo-Hee
    • 한국사회복지학회:학술대회논문집
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    • 2004.04a
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    • pp.757-779
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    • 2004
  • The purpose of study is to provide efficient initial approaches for counseling in social work practice settings. First, it attempts to identify the correlation between the Myers-Brigg's Type Indicator(MBTI), a personality index based on the theory of psychological typology, and Personality Adaptation Types(PAT) models. Second, it attempts to develop a counseling method which can be applied to the initial interview, using the paradigm of door to therapy such as "Open Door", "Target Door", and "Trap Door" based on the PAT theory. The result of the quantitative study showed the correlation between MBTI and PAT as follows: MBTI Extrovert and Feeling(E, F) type was statistically correlated to hysterical PAT. MBTI Extrovert, Intuition and Perceiving(E, N, P) type was statistically correlated to Obsessive-Compulsive PAT. In conclusion, this study finds that when a clinical social worker approaches a client with the client's MBTI personality type and an interventional personality adaptation theory approach such as "Open Door", "Target Door", or "Trap Door", the efficiency of communication is increased significantly.

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