Browse > Article
http://dx.doi.org/10.5012/bkcs.2005.26.3.379

Hybridization by an Electrical Force and Electrochemical Genome Detection Using an Indicator-free DNA on a Microelectrode-array DNA Chip  

Choi, Yong-Sung (School of Electrical, Electronics and Information of Engineering, WonKwang University)
Lee, Kyung-Sup (Dept. of Electrical Eng., Dongshin University)
Park, Dae-Hee (School of Electrical, Electronics and Information of Engineering, WonKwang University)
Publication Information
Bulletin of the Korean Chemical Society / v.26, no.3, 2005 , pp. 379-383 More about this Journal
Abstract
This research aims to develop DNA chip array without an indicator. We fabricated microelectrode array by photolithography technology. Several DNA probes were immobilized on an electrode. Then, indicator-free target DNA was hybridized by an electrical force and measured electrochemically. Cyclic-voltammograms (CVs) showed a difference between DNA probe and mismatched DNA in an anodic peak. Immobilization of probe DNA and hybridization of target DNA could be confirmed by fluorescent. This indicator-free DNA chip microarray resulted in the sequence-specific detection of the target DNA quantitatively ranging from $10^{-18}\;M\;to\;10^{-5}$ M in the buffer solution. This indicator-free DNA chip resulted in a sequence-specific detection of the target DNA.
Keywords
Indicator-free DNA chip; Immobilization; Target DNA; Hybridization; Ferricyanide;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 4  (Related Records In Web of Science)
Times Cited By SCOPUS : 4
연도 인용수 순위
1 Pividori, M. I.; Merko, A.; Alegret, S. Biosens. Bioelectron. 2000, 15, 291   DOI   ScienceOn
2 Choi, Y. S.; Park, D. H.; Kwon, Y. S.; Tomoji, T. Trans. KIEE 2003, 52C, 365
3 Kim, D. K.; Choi, Y. S.; Murakami, Y.; Tamiya, E.; Kwon, Y. S. KIEE Inter. Trans. on EA 2001, 11C, 85
4 Johnston, D. H.; Glasgow, K. C.; Thorp, H. H. J. Am. Chem. Soc. 1995, 117, 8933   DOI   ScienceOn
5 O'Donnell, M. J.; Tang, K.; Koster, H.; Smith, C. L.; Cantor, C. R. Anal. Chem. 1997, 69, 2438   DOI   ScienceOn
6 Pease, A. C.; Solas, D.; Sullivan, E. J.; Cronin, M. T.; Holmes, C. P.; Fodor, S. P. A. Proc. Natl. Acad. of Sci. U.S.A. 1994, 91, 5022
7 Steel, A. B.; Herne, T. M.; Tarlov, M. J. Anal. Chem. 1998, 70, 4670   DOI   ScienceOn
8 Kim, D. J.; Oh, D. B.; Lee, S. M.; Choi, I. S.; Kim, Y. G. Bull. Korean Chem. Soc. 2004, 25, 1430   DOI   ScienceOn
9 Schena, M.; Shalon, D.; Heller, R.; Chai, A.; Brown, P. O.; Davis, R. W. Proc. Natl. Acad. Sci. USA 1996, 93, 10614   ScienceOn
10 Schena, M.; Shalon, D.; Davis, R. W.; Brown, P. O. Science 1995, 270, 467   DOI   ScienceOn
11 Choi, Y. S.; Kim, D. K.; Kwon, Y. S. KIEE Inter. Trans. on EA 2001, 11C, 23
12 Cho, S. B.; Pak, J. J.; Hong, J. S.; Kimpak, Y. M. J. Korean Phys. Soc. 2002, 41, 1054
13 Cho, S. B.; Hong, J. S.; Pak, Y. K.; Pak, J. J. J. Korean Phys. Soc. 2002, 40, 17
14 Johnson, D. H.; Glasgow, K. C.; Thorp, H. H. J. Am. Chem. Soc. 1995, 117, 8933   DOI   ScienceOn
15 Ropp, P. A.; Thorp, H. H. Chem. Biol. 1999, 6, 599   DOI   ScienceOn
16 Park, N. K.; Hahn, J. H. Anal. Chem. 2004, 76, 900   DOI   ScienceOn
17 Lee, B. S.; Lee, S. G. Bull. Korean Chem. Soc. 2004, 25, 1531   DOI   ScienceOn
18 Kim, H. K.; Yang, C. H. Bull. Korean Chem. Soc. 2004, 25, 1769   DOI   ScienceOn
19 Choi, J. S.; Choi, Y. H.; Park, J. S. Bull. Korean Chem. Soc. 2004, 25, 1025   DOI   ScienceOn
20 Southern, E. M.; Case-Green, S. C.; Elder, J. K.; Johnson, M.; Mir, K. U.; Wang, L.; Williams, J. C. Nucleic Acids Res. 1994, 22, 1368   DOI   ScienceOn