• Genomics & Informatics
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• 제21권3호
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• pp.39.1-39.15
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• 2023

DNA barcoding without assessing reliability and validity causes taxonomic errors of species identification, which is responsible for disruptions of their conservation and aquaculture industry. Although DNA barcoding facilitates molecular identification and phylogenetic analysis of species, its availability in clariid catfish lineage remains uncertain. In this study, DNA barcoding was developed and validated for clariid catfish. 2,970 barcode sequences from mitochondrial cytochrome c oxidase I (COI) and cytochrome b (Cytb) genes and D-loop sequences were analyzed for 37 clariid catfish species. The highest intraspecific nearest neighbor distances were 85.47%, 98.03%, and 89.10% for COI, Cytb, and D-loop sequences, respectively. This suggests that the Cytb gene is the most appropriate for identifying clariid catfish and can serve as a standard region for DNA barcoding. A positive barcoding gap between interspecific and intraspecific sequence divergence was observed in the Cytb dataset but not in the COI and D-loop datasets. Intraspecific variation was typically less than 4.4%, whereas interspecific variation was generally more than 66.9%. However, a species complex was detected in walking catfish and significant intraspecific sequence divergence was observed in North African catfish. These findings suggest the need to focus on developing a DNA barcoding system for classifying clariid catfish properly and to validate its efficacy for a wider range of clariid catfish. With an enriched database of multiple sequences from a target species and its genus, species identification can be more accurate and biodiversity assessment of the species can be facilitated.

• 수산해양기술연구
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• 제54권3호
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• pp.224-230
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• 2018

Squid is one of the important fisheries resources in Korea. Therefore, squid has been designated and managed as a target species of total allowable catch (TAC) since 2007, but the catch amount is gradually decreasing. The analysis was conducted to identify the change of relative fishing power index to develop the vessel and gear technology that may have improved the fishing efficiency of the offshore squid jigging fishery from 1960s to 2010s. Gross tonnage per fishing vessel increased with the increase in size until 1990, but then gradually decreased to 41.0 tons in 2000 and 37.1 tons in 2010. The illuminating power (energy consumption) by fishing lamps increased to 180 kW in 2005 and stabilized to 120 kW in 2015. Jigging machine started to be supplied to fishing vessels from the early 1970s, and fish finders began to be supplied in the early 1980s and gradually increased. Therefore, the relative fishing power index in the offshore squid jigging fishery increased from 1.0 in 1980 to 1.1 in 1990, to 3.5 in 2000 and to 2.5 in 2010, but the increment rate slowed down gradually. The results are expected to contribute to reasonable fisheries stock management.

• 수산해양기술연구
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• 제45권1호
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• pp.1-13
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• 2009

Experimental fishing operation by the shrimp beam trawl was carried out to investigate the bycatch in order to develop the selective fishing gear of the shrimp beam trawl fishery. The experimental trawling was performed by the prototype fishing gear in coast sea of Boryeong, Buan, Kangjin, Tongyeong and Geoje from 30 April to 13 November 2005. Bycatches of the experimental trawling were analyzed species composition, total length composition. The results obtained can be summarized as follows; Target fishes caught by the shrimp beam trawl were Palaemon gravieri, Crangon Hakodatei, Trachysalambria curvirostris, Parapenaeopsis tenellus, Rhynchocinetes uritai and Matapenaeus joyneri. And their fishing rate and total length range were 50.0%, 46.0%, 2.6%, 0.4%, 0.3%, 0.3% and 40-80mm, 40-80mm, 120-150mm, 60-80mm, 60-80mm, 60-120mm respectively. Bycatches caught by the shrimp beam trawl consisted of fishes of 30 kinds, 6 kinds of mollusk, 5 kinds of shellfishes and 3 kinds of crabs. The main fish was Engraulis japonicus, Platycephalus indicus, Paralichthys olivaceus, Leiognathus nuchalis and Conger myriaster, their occupied rate were 47.0%, 16.6%, 13.6%, 5.5%, 2.7% respectively, and their total length range were 60-150mm, 80-410mm, 80-600mm, 30-80mm and 150-460mm respectively. A number bycatch rates of shrimp beam trawl fishery were 99.7% in Buan, 60.0% in Kangjin, 14.6% in Boryeong, 10.6% in Tongyeong and 2.7% in Geoje, The weight bycatch rate were 99.9% in Buan, 75.1% in Kangjin, 57.1% in Tongyeong, 47.4% in Boryeong and 15.4% in Geoje.

• 수산경영론집
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• 제48권2호
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• pp.19-32
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• 2017

The purpose of this study is to identify problems and suggest improvements of estimating procedures and item of fisheries supply-demand statistics served as a basis for the fisheries supply-demand policies. Korea Rural Economic Institute(KREI) and Ministry of Oceans and Fisheries(MOF) respectively publish the fisheries supply-demand statistics. But the reliability of data is low as the statistics of these two organizations are limited and show discrepancy in the numbers. It is therefore difficult to use them as the basic data for policies. Also, an accurate data aggregation is difficult due to following problems in the items of statistics. 1) Problems in estimating route sales and non-route sales of production, 2) adequacy of fishery product yield rate compared to raw material in the fisheries import/export sector, 3) selection of target companies for understand stocks and survey scope of fish species, 4) applying'0'to non-edible product demand etc. In order to develop the fisheries industry as a future growth industry, it is necessary to establish the accurate fisheries supply-demand policy as the instability of fisheries supply and demand is increasing. To do this, statistical reliability has to be improved. The improvements proposed in this study should be implemented considering urgency. First of all, an exhaustive analysis of stock statistics and conversion rates of raw material yield in the fisheries import/export sector should be conducted. In the medium term and the long term, transferring production statistics to MOF and surveys on the use demand of non-food product and the level of reduced and discarded seafood products should be carried out in consecutive order.

• 수산해양기술연구
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• 제48권2호
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• pp.107-117
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• 2012

Korean large purse seine fishery catches chub mackerel, sardine, jack mackerel, Spanish mackerel, etc. which are mainly pelagic fish species. The proportion of chub mackerel was 60% over in Korean large purse seine fishery. Sea surface temperature (SST) increased $0.0253^{\circ}C$ per year and total rising rate was $0.759^{\circ}C$ from 1980 to 2009 in the southern sea of Korea, where is mainly fishing grounds of Korean large purse seine. It was that p<0.01 level was statistically significant. It is northward movement that the center of fishing grounds of chub mackerel by Korean large purse seine fishery moved 4.57km/yr. It was rapidly northward movement about 7.1km/yr, 8.13km/yr to move Spanish mackerel and bluefin tuna fishing grounds. However, the fishing grounds of jack mackerel were moved further south in the 2000s than the 1980s. Catch of tunas and bluefin tuna consistently increased in Korean waters. There was a significantly positive correlation between SST and catch of bluefin tuna in the fishing grounds of Korean waters.

• 수산해양기술연구
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• 제58권2호
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• pp.95-105
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• 2022

In this study, the fishery status of the octopus pot fishery in the east coastal sea were investigated, and the fishing performance of each pot shape was compared and analyzed. The fishery status survey was conducted through listening surveys at Jukbyeon Port, Uljin Port and Pohang Daebo Port in Gyeongbuk Province, and the amount of fishing gear used, fishing method, size and loss of octopus pot fishery was investigated. On the east coastal sea, octopus is one of the commercially important fish stocks and is caught in inshore pots, inshore combos, inshore gillnets and offshore pots. Among these fishing methods, pot fishing yields the highest catch. The shape of the pot differs depending on the region. In Uljin (Jukbyeon Port and Hupo Port), Gyeongbuk, rectangular type net pots are mainly used, and in Pohang (Daebo Port) in Gyeongbuk, drum-type pots are mostly used. Enteroctopus dofleini accounts for more than 90% of the catch of octopus. For the octopus fishing performance test by trap type, three types of traps (rectangular pot, drum pot and cylinder pot) were used on the coastal sea of Pohang Daebo. As a result, the total catch by pot shape was shown in the order of rectangular-type pot > drum-type pot > cylinder-type pot. The catch of octopus, the target species, was in the following order: rectangular-type pot > drum-type pot > cylinder-type pot. Such result shows a significant difference (Mann-Whitney test, p<0.05).

• 한국식품위생안전성학회지
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• 제30권1호
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• pp.43-50
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• 2015

본 연구에서는 오징어류에 해당하는 대왕오징어, 오징어, 문어, 한치 및 이를 이용한 가공식품에 대해서 분자생물학적 기법을 활용한 시험법을 검토하였다. 시료 중 원료성분 확인을 위하여 오징어류 4종에 대해 최적의 종 특이 프라이머를 디자인하였으며, 시료로부터 직접 genomic DNA를 추출하여 PCR을 실시하였다. PCR 수행과정에서 반응을 저해하는 염 성분을 제거하기 위하여 증류수를 이용하여 3~4회 세척 후 PCR을 실시한 결과, Single PCR의 경우 대왕오징어(552 bp), 오징어(463 bp), 문어(247 bp), 한치(354 bp)에 해당하는 종 특이적인 증폭산물을 확인하였으며, Multiplex PCR 의 경우 서로 다른 종 사이의 교차반응없이 동시다발적으로 증폭이 일어남을 확인할 수 있었다. 또한 이들 4종에 대해 PCR 민감도를 조사한 결과, 모두 약 $0.1ng/{\mu}l$의 농도까지 검출이 가능함을 확인하였으며 multiplex PCR의 경우 약 $0.25ng/{\mu}l$의 농도까지 검출이 가능함을 확인하였다. 이를 이용하여 오징어류가 함유된 수산물 가공식품 8건에 대해 적용성을 검토한 결과, 모든 시료에서 유효한 결과를 확인할 수 있었다. 따라서 본 연구에서 제작된 오징어류 4종에 대한 종 특이적 프라이머는 생물 상태뿐만 아니라 수산물 가공식품에 대해서도 이를 판별할 수 있어 식품안전관리에 활용할 수 있을 것으로 기대된다.

• Ecology and Resilient Infrastructure
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• 제3권4호
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• pp.285-293
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• 2016

본 연구는 원주천을 대상으로 하천시설물의 최적 설계를 위한 안정하도 평가와 물리적 교란개선 평가를 수행하였다. 안정하도 설계를 위한 구간별 안정경사를 분석하였으며, 안정경사를 만족하기 위한 하천시설물의 개선 시나리오를 도출하였다. 그에 따라 각 시나리오에 대한 물리적 교란개선 평가를 통해 안정하도 설계와 물리적 교란개선을 위한 최적의 시나리오를 선정하였다. 아울러 안정하도 설계와 물리적 교란개선을 위한 하천시설물의 개선 시 물리 서식처의 변화를 분석하였다. 원주천의 하천 환경조사와 어류의 군집특성을 이용하여 수중 생태계를 대표할 수 있는 복원 목표어종으로 참갈겨니를 선택하였다. 참갈겨니의 서식적합지수를 사용한 서식적합도 분석은 PHABSIM 모형을 이용하였다. 예측 평가를 수행한 결과 서식적합도와 가중가용면적은 소폭 감소하는 것으로 나타났으나, 하천시설물의 개선에 따른 영향은 아닌 것으로 나타났다. 따라서 원주천의 물리적 건전성과 안정성을 제고할 수 있는 하천시설물의 배치 방안을 제시하였으며, 그에 따른 물리 서식처의 변화는 영향이 없는 것을 확인하였다.

• 수산해양기술연구
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• 제51권3호
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• pp.348-354
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• 2015

Experiments for catch and cluster analysis on the catch by gillnet for the Pacific herring were conducted using traditional nylon net (PA) and developing biodegradable net (PBS) with different monofilaments of #2 (=0.23 mm) and #3 (=0.28 mm) in diameter between 2011 and 2012, and of #2, #2.5 and #3 in 2013 in the eastern coastal waters of Samcheok, Korea. The total catch during the experiments was 3,337kg in weight and 16,757 individuals in number. Though the catch ratio of the Pacific herring in 2011 and 2012 was 97.4% and 98.7%, respectively, that of the Pacific herring in 2013 was 73.6% as the biggest and followed by goldeye rockfish (8.6%), black edged sculpin (8.4%), atka mackerel (7.2%) and Alaska cod (1.0%). The ANOVA analysis on the total catch in relation to net material showed that there was not statistically significant difference at the 5% level between nylon net (PA) and biodegradable net (PBS) (p=0.91). With the cluster analysis on the catch in relation to net material (PA, PBS) and twine thick (#2, #3), it showed the influence of net material on the catch was greater than that of twine diameter in this experiment. From the test of homogeneity on the catch by the gillnet with PA and PBS in which several species were caught in 2013, there was not statistically significant homogeneity in relation to net material (p<0.005). That meant the traditional gillnet of nylon (PA) was more efficient than the biodegradable (PBS) gillnet to catch several kinds of fish, however the PBS gillnet was better than the PA gillnet to catch the target Pacific herring.

• 생태와환경
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• 제48권2호
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• pp.77-85
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• 2015

쏘가리는 국내 주요 경제성 어종이며 방류 및 복원사업의 대상 어종 중 하나이다. 본 연구는 금강의 보 설치구간에서 쏘가리의 이동 특성 및 서식처 범위 등을 평가하기 위해 Radio telemetry를 이용하여 연구를 실시하였다. 연구결과 산란기에 방류한 개체들의 경우 방류 즉시 상류로 10km 이상 소상하였지만, 비 산란기에 방류한 개체들은 2개체를 제외한 모든 개체들이 방류 지점으로부터 400 m 이내에서 정착하였다 (Ss11, 3.2 km; Ss 15, 1.4 km). 산란기에 상류로 소상한 개체들이 정착한 지점의 서식처 특성은 기존에 알려진 쏘가리의 산란장 특성과 유사하게 나타났으며, 현재 퇴적이 진행되고 있는 상태였다. 쏘가리가 감지는 수변부에서 높은 비율로 나타났으며, 이는 수변부에 큰 돌이 많이 분포하는 것, 쏘가리가 주로 돌 틈에 숨어서 서식하는 특성과 관련이 있는 것으로 판단된다. 쏘가리의 개체 특성과 이동거리와의 상관관계 분석 결과 무게와 비대지수가 이동거리와 양의 상관관계를 보였으며 성별과 크기는 이동거리와 관련이 없는 것으로 나타났다. 일주기 이동 범위 분석 결과$214.94m^2{\sim}3,257.19m^2$로 나타났으며 주로 수변부에 국한되어 있는 것으로 확인되었다. 이와 같은 결과는 쏘가리 방류사업, 혹은 복원에 유용한 자료로 활용될 수 있을 것으로 판단된다.