• Journal of the Korea Institute of Military Science and Technology
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• v.20 no.2
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• pp.238-245
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• 2017

This paper deals with the scale effects of warhead on concrete penetration. We investigated the scale effects using finite element analysis and Young's penetration equation. As the scale of penetration test decreases, the strain rate effects of target increases, and then strength of concrete target increases. This means the residual velocity and penetration depth of warhead decreases as the test model size decreases. Young's penetration equations are transformed with various penetrator mass and scale cases as a function of scale ratio. Penetration distance and residual velocity are not simply changed by the geometric scaling law.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.411-414
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• 2002

Acylated homoserine lactones (AHLs) are membrane-permeant signal molecules responsible for biofilm formation of gram-negative bacteria via a unique mechanism known as quorum sensing. A target specific bioassay employing the AHL-responsive Agrobacterium tumefaciens reporter strain has been developed to identify new AHL-like compounds from natural products, which could be developed into antifouling compounds. By varying the X-gal concentration, incubation time, solvent for sample preparation and the sample loading procedure, it was possible to detect low level AHLs up to $10^1nM$. The length of the acyl chain of the AHLs was found to affect the sensitivity of this bioassay.

• Korean Journal of Veterinary Research
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• v.49 no.3
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• pp.221-229
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• 2009

Foot-and-mouth disease (FMD) is the most contagious disease of mammals. The use of inactivated vaccine can be chosen to prevent or control FMD. However, vaccination against one serotype of FMDV doses not cross-protect against other serotypes and may not protect fully against some strains of the same serotype. Appropriate selection of vaccine strain is an important element in the control of FMD. The immunity of vaccine antigens should be matched against newly circulating viruses. The phylogenetic analysis of serotype Asia1 reported from China, Mongolia, North Korea and Russia since 2005 shows that they are all classified into genetic group V, but the strain, Asia1/Shamir (ISR/89) which have been used as a vaccine strain in Korea, is clustered into different genetic group. So, in this study the serological relationship between the isolate (Asia1/MOG/05; MOG) and the Shamir strain was determined by ELISA and virus neutralization test. Even though the matching value of the virus (MOG) against the vaccinated sera in target animals was not so high, the vaccinated animals elicited antibodies enough for protection after vaccinated once or twice. Conclusively, we suggest that the vaccine containing Asia1/Shamir antigen could protect the genetic group V strains circulating in East Asia currently if vaccinated twice or the more.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.19 no.3 s.73
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• pp.313-322
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• 2006

In the genetic algoricm (GA) based damage detection methods using vibration of structures, the selection of modal properties is important to improve the accuracy of damage detection. The objective of this study is to improve the accuracy of damage detection using natural frequency and modal strain energy, The following approaches are used to achieve the goal. First, modal strain energy is formulated and a new GA-based damage detection technique using natural frequency and modal strain energy is proposed. Next, to verify the efficiency of proposed technique, damage scenarios for free-free beam are designed and vibration modal tests of the target structure are conducted. Finally, the feasibility of the proposed technique is verified in comparison with other GA-based damage detection technique using natural frequency and mode shape.

• Journal of the Earthquake Engineering Society of Korea
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• v.13 no.3
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• pp.67-80
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• 2009

The basic concept of seismic design is to attain the ductility required in a design earthquake. This ductility can be obtained by providing sufficient lateral confinements to the plastic hinge regions of columns. The most cost-effective design might be derived by determining the proper amount of lateral confinement using a stress-strain relationship for confined concrete. Korean bridge design code requires the same amount of lateral confinement regardless of target ductility, but Japanese design code provides the stress-strain relationship of the confined concrete to determine the amount of lateral confinement accordingly. While design based on material characteristics tends to make the design process more involved, it makes it possible to achieve cost-effectiveness, which is also compatible with the concept of performance-based design. In this study, specimens with different numbers of lateral confinements have been tested to investigate the characteristics of the stress-strain relationship. Test results were evaluated, using several empirical equations to quantify the effects.

• Journal of the Korean Recycled Construction Resources Institute
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• v.8 no.4
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• pp.458-466
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• 2020

The purpose of this experimental research is to evaluate the compressive and tensile behaviors of high performance hybrid fiber reinforced concrete(HPHFRC) using amorphous steel fiber(ASF) and polyamide fiber(PAF). For this purpose, the HPHFRCs using ASF and PAF were made according to their total volume fraction of 1.0% for target compressive strength of 40MPa and 60MPa, respectively. And then the compressive and tensile behaviors such as the compressive strength, compressive toughness, direct tensile strength, and stress-strain characteristics under compressive and tensile tests were estimated. It was observed from the test results that the compressive strength of HPHFRC was slightly decreased than that of plain concrete, but the compressive toughness, compressive toughness ratio, and direct tensile strength of HPHFRC increased significantly. Also, it was revealed that the plain concrete showed brittle fracture after the maximum stress from the stress-strain curves, but HPHFRC showed strain softening.

• Journal of Microbiology and Biotechnology
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• v.33 no.2
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• pp.167-179
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• 2023

The rifampicin-resistant strain E9-302 of Edwardsiella ictaluri strain 669 (WT) was generated by continuous passage on BHI agar plates containing increasing concentrations of rifampicin. E9-302 was attenuated significantly by 119 times to zebrafish Danio rerio compared to WT in terms of the 50% lethal dose (LD₅₀). Zebrafish vaccinated with E9-302 via intraperitoneal (IP) injection at a dose of 1 × 10³ CFU/fish had relative percentage survival (RPS) rates of 85.7% when challenged with wild-type E. ictaluri via IP 14 days post-vaccination (dpv). After 14 days of primary vaccination with E9-302 via immersion (IM) at a dose of 4 × 10⁷ CFU/ml, a booster IM vaccination with E9-302 at a dose of 2 × 10⁷ CFU/ml exhibited 65.2% RPS against challenge with wild-type E. ictaluri via IP 7 days later. These results indicated that the rifampicin-resistant attenuated strain E9-302 had potential as a live vaccine against E. ictaluri infection. A previously unreported amino acid site change at position 142 of the RNA polymerase (RNAP) β subunit encoded by the gene rpoB associated with rifampicin resistance was identified. Analysis of the whole-genome sequencing results revealed multiple missense mutations in the virulence-related genes esrB and sspH2 in E9-302 compared with WT, and a 189 bp mismatch in one gene, whose coding product was highly homologous to glycosyltransferase family 39 protein. This study preliminarily explored the molecular mechanism underlying the virulence attenuation of rifampicin-resistant strain E9-302 and provided a new target for the subsequent study of the pathogenic mechanism of E. ictaluri.

• Microbiology and Biotechnology Letters
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• v.52 no.1
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• pp.44-54
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• 2024

One of the serious modern environmental problems is pollution caused by highly toxic pesticides. Only small amounts of applied pesticides reach their target, and the rest ends up in soil and water. Chlorpyrifos is a toxic, broad-spectrum organophosphate insecticide. In humans, chlorpyrifos inhibits acetylcholinesterase (AChE) in the peripheral and central nervous system, and particularly in children, small amounts of this pesticide cause neurotoxic damage. As the toxic effects of chlorpyrifos and its persistence in the environment require its removal from contaminated sites, it is essential to study the biological diversity of chlorpyrifos-degrading microorganisms. In this study, we sought to determine the chlorpyrifos-degrading ability of the bacterial strain Ochrobactrum intermedium PDB-3. This strain was isolated from soil contaminated with various pesticides and identified as PDB-3 based on morpho-cultural characteristics, MALDI-TOF MS, and 16S rRNA. Studies were conducted for 30 days in sterile soils containing initial concentrations of 50, 75, 100, and 125 mg/kg of chlorpyrifos. To determine the degradation of chlorpyrifos, a liquid culture of the strain was added to the soil at three optical densities: 0, and after 24 and 48 h (OD = 0.03, 0.2 and 0.32). Using GX-MS, we determined that chlorpyrifos was converted to 3,5,6-trichloro-2-pyridinol (TCP). We also found that with increasing optical density, rapid degradation of the initial concentration of chlorpyrifos occurred. Sterile soil without strain PDB-3 was used as a control sample.

• Corrosion Science and Technology
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• v.23 no.4
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• pp.302-309
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• 2024

The purpose of this study was to elucidate effects of a thin (tens to hundreds of nanometers) Ni-flash coating layer on hydrogen embrittlement (HE) and liquid metal embrittlement (LME) in ultra-high-strength electrogalvanized steel with a tensile strength of more than 1 GPa. Various experimental and analytical methods, including thermal desorption spectroscopy, slow strain rate testing, resistance spot welding, X-ray diffraction, and metallographic observation, were employed. Results showed that an increase in Ni target amount for flash coating resulted in a decrease in diffusible hydrogen content during electrogalvanizing, resulting in a significant decrease in HE sensitivity. Moreover, a Ni target amount of more than 1000 mg/m² drastically reduced the occurring frequency and average depth of LME. This reduction could be primarily attributed to formation of Zn-Ni intermetallic phases during the welding process that could inhibit liquefaction of intermetallic phases in the heat-affected zone. This study provides a desirable Ni target amount for Ni-flash coating on ultra-high-strength steels conducted in a continuous galvanizing line or a high-speed batch line to achieve high resistance to both HE and LME.

• Proceedings of the Korea Concrete Institute Conference
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• 2006.05b
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• pp.633-636
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• 2006

Impact damage modeling of concrete under high strain rate loading conditions is investigated. A phenomenological penetration model that can account for complicated impact and penetration process such as the rate and loading history response of concrete, the microstructure-penetration interaction etc. is discussed. Constitutive law compatible with Second Law of thermodynamics and coupled damage and plasticity modelling based on continuum damage mechanics are also examined. The purpose of this paper is preliminarily to study with respect to impact and penetration models for concrete before the development of that model.