• Fire Science and Engineering
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• v.33 no.1
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• pp.85-91
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• 2019

This study evaluated the fire detection performance of an automatic fire extinguishing system for road tunnels, which combines flame wavelength detection technology with flame image detection technology. This fusion technique to improve the fire detection capability can reduce the damage caused by the fire suppression by locating the fire source in the fire and discharging the pressurized water only at the fire source. Experiments were conducted to determine the position of a fire source when a $70cm{\times}70cm$ target was placed at a distance of 15 m, 20 m, 25 m, 30 m, and 35 m, respectively, in a situation where there is a flame and smoke in a tunnel. The performance of the ultraviolet and triple wavelength infrared (IR3) sensors was attenuated due to the interference of thick smoke. In addition when the flame was blocked by thick smoke, the image sensor sensed the smoke and emitted a fire signal.

• Biomolecules & Therapeutics
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• v.29 no.1
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• pp.73-82
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• 2021

Hsp90 is often overexpressed with activated form in cancer cells, and many key cellular proteins are dependent upon the Hsp90 machinery (these proteins are called "client protein"). Nowadays, more client proteins and more inhibitors of Hsp90 are being discovered. Chaetocin has been identified as an inhibitor of histone methyl transferase SUV39H1. Herein, we find that Chaetocin is an inhibitor of Hsp90 which binds to the C-terminal of Hsp90α. Chaetocin inhibited a variety of Hsp90 client proteins including AMl1-ETO and BCL-ABL, the mutant fusion-protein in the K562 and HL-60 cells. SUV39H1 mediates epigenetic events in the pathophysiology of hematopoietic disorders. We found that inhibition of Hsp90 by Chaetocin and 17-AAG had ability to induce degradation of SUV39H1 through proteasome pathway. In addition, SUV39H1 interacted with Hsp90 through co-chaperone HOP. These results suggest that SUV39H1 belongs to a client protein of Hsp90. Moreover, Chaetocin was able to induce cell differentiation in the two cells in the concentration range of Hsp90 inhibition. Altogether, our results demonstrate that SUV39H1 is a new client protein of Hsp90 degradated by Chaetocin as a novel C-terminal inhibitor of Hsp90. The study establishes a new relationship of Chaetocin and SUV39H1, and paves an avenue for exploring a new strategy to target SUV39H1 by inhibition of Hsp90 in leukemia.

• Journal of Microbiology and Biotechnology
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• v.32 no.6
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• pp.816-823
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• 2022

The rapid spread of superbugs leads to the escalation of infectious diseases, which threatens public health. Endolysins derived from bacteriophages are spotlighted as promising alternative antibiotics against multi-drug resistant bacteria. In this study, we isolated and characterized the novel Salmonella typhimurium phage PBST08. Bioinformatics analysis of the PBST08 genome revealed putative endolysin ST01 with a lysozyme-like domain. Since the lytic activity of the purified ST01 was minor, probably owing to the outer membrane, which blocks accessibility to peptidoglycan, antimicrobial peptide cecropin A (CecA) was fused to the N-terminus of ST01 to disrupt the outer membrane. The resulting CecA::ST01 has been shown to have increased bactericidal activity against gram-negative pathogens including Pseudomonas aeruginosa, Klebsiella pneumoniae, Acinetobacter baumannii, Escherichia coli, and Enterobacter cloacae and the most affected target was A. baumannii. In the presence of 0.25 µM CecA::ST01, A. baumannii ATCC 17978 strain was completely killed and CCARM 12026 strain was wiped out by 0.5 µM CecA::ST01, which is a clinical isolate of A. baumannii and resistant to multiple drugs including carbapenem. Moreover, the larvae of Galleria mellonella could be rescued up to 58% or 49% by the administration of CecA::ST01 upon infection by A. baumannii 17978 or CCARM 12026 strain. Finally, the antibacterial activity of CecA::ST01 was verified using 31 strains of five gram-negative pathogens by evaluation of minimal inhibitory concentration. Thus, the results indicate that a fusion of antimicrobial peptide to endolysin can enhance antibacterial activity and the spectrum of endolysin where multi-drug resistant gram-negative pathogens can be efficiently controlled.

• The Journal of Korea Robotics Society
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• v.18 no.3
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• pp.251-259
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• 2023

In the path traveling of differential-drive robots, the steering controller plays an important role in determining the path-following performance. When a robot with a pure-pursuit algorithm is used to continuously drive a right-angled driving path in an unstructured environment without turning in place, the robot cannot accurately follow the right-angled path and stops driving due to the ground and motor load caused by turning. In the case of pure-pursuit, only the current robot position and the steering angle to the current target path point are generated, and the steering component does not reflect the speed plan, which requires improvement for precise path following. In this study, we propose a driving algorithm for differentially driven robots that enables precise path following by planning the driving speed using the radius of curvature and fusing the planned speed with the steering angle of the existing pure-pursuit controller, similar to the Model Predict Control control that reflects speed planning. When speed planning is applied, the robot slows down before entering a right-angle path and returns to the input speed when leaving the right-angle path. The pure-pursuit controller then fuses the steering angle calculated at each path point with the accelerated and decelerated velocity to achieve more precise following of the orthogonal path.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.17 no.8
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• pp.2278-2291
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• 2023

Imported ornamental fish should be quarantined because they can have dangerous diseases depending on their habitat. The quarantine requires a lot of time because quarantine officers collect various information on the imported ornamental fish. Inefficient quarantine processes reduce its work efficiency and accuracy. Also, long-time quarantine causes the death of environmentally sensitive ornamental fish and huge financial losses. To improve existing quarantine systems, information on ornamental fish was collected and structured, and a server was established to develop quarantine performance support software equipped with a text search engine. However, the long names of ornamental fish in general can cause many typos and time bottlenecks when we type search words for the target fish information. Therefore, we need a technique that can correct typos. Typical typo character calibration compares input text with all characters in a calibrated candidate text dictionary. However, this approach requires computational power proportional to the number of typos, resulting in slow processing time and low calibration accuracy performance. Therefore, to improve the calibration accuracy of characters, we propose a fusion system of simple Artificial Neural Network (ANN) models and character preprocessing methods that accelerate the process by minimizing the computation of the models. We also propose a typo character generation method used for training the ANN models. Simulation results show that the proposed typo character correction system is about 6 times faster than the conventional method and has 10% higher accuracy.

• The Journal of the Convergence on Culture Technology
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• v.10 no.5
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• pp.497-503
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• 2024

This paper makes a comparative analysis of themes, appeals, and aims of TV public service advertisements through 927 advertisements from China and South Korea. The study found that there is an obvious difference between China and Korea TV public service advertisements : (1) China TV public service advertisements pay more attention to public morals, while advertisements of South Korea focus on national fusion ; (2) In the way of appeal, different from China's rational demands, South Korea is based on emotional appeals ; (3) The aim of Chinese public service advertisements is for public but South Korea's target is for individuals. Thus, although both China and South Korea are Asian countries, their TV public service advertisements are obvious different. So it is necessary to analyze different situations of the two countries when China and South Korea focus on the same theme of publicity.

• Korean Journal of Microbiology
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• v.38 no.3
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• pp.153-161
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• 2002

Sox4, a transcription factor, consists of three functional domains: an HMG-box domain as a DNA binding domain, serine rich region as a transactivation domain and glycine rich region (GRR), an unknown functional domain. Although Sox4 is known to be functionally involved in heart, B-cell and reproductive system development, its physiological function remains to be elucidated. We used pGEX expression system to develop a simple and rapid method for purifying Sox4 protein in suitable forms for biochemical studies of their functions. Unexpectedly, we observed that full-length Sox4 appears to be protease-sensitive during expression and purification in E. coli. To map the protease-sensitive site in Sox4, we generated various constructs with each of functional domains of Sox4 and purified as the GST-Sox4 fusion proteins using glutathione beads. We found that the specific cleavage site for the proteolytic enzyme, which exists in E. coli, is localized within the novel GRR of Sox4. Our study suggest that the GRR of Sox4 may a target for the cellular protease action and this cleavage in the GRR may be involved in regulating physiological function of Sox4. Additionally, our study may provide a useful method for investigating the proteolytic cleavage of the target molecule in E. coli.

• Journal of the Korean Magnetics Society
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• v.26 no.5
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• pp.173-178
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• 2016

This study is to evaluate the effect of a Contrast Media (CM) on dose calculations and clinical significance in Radiation (Electromagnetic wave) Therapy (RT) plans for head & neck (H&N) and prostate cancer. Pinnacle 8.0 system was used to measure the change of Electron Density (ED) of the tissue for CM. To determine the effect of dose calculation due to CM, we did the RT planning for 30 patients. To compare the ED and dose calculations of RT plans, 3D CRT and IMRT plans were do with pinnacle and Tomotherapy planning system. Mean difference of ED between enhanced and unenhanced CT was less than 4%: H&N Target Volume (TV) 2.1%, parotid 1.9%, SMG 3.6%, tongue 0.9%, spinal cord 0.3%, esophagus 2.6%, mandible 0.1% and prostate TV 0.7%, lymph node 1.1%, bladder 1.2%, rectum 1.5%, small bowel 1.2%, colon 0.6%, penile bulb 0.8%, femoral head -0.2%. The dose difference between RT plan using CM and without CM showed an increase of dose in TV. The rate of increase was less than 2.5% (3D CRT: H&N 0.69~2.51%, prostate 0.04~1.14%, IMRT: H&N 0.58~1.31%, prostate 0.36~1.04%). RT plans using a CM has the insignificant effect on the organs and TV, so this error is allowable clinically. However, the much more accurate plan is possible as to image fusion (CM and without CM images) to ROI contour and when dose calculation, use the without CM image. Using the fusion of 'ROI import' perform calculations on without CM, it will be able to reduce the error (1~3%) caused by the CM.

• KIPS Transactions on Computer and Communication Systems
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• v.10 no.8
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• pp.223-230
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• 2021

The lightweight block cipher PIPO announced at ICISC'20 has been effectively implemented by applying the bit slice technique. In this paper, we propose a parallel optimal implementation of PIPO for ARM processors. The proposed implementation enables parallel encryption of 8-plaintexts and 16-plaintexts. The implementation targets the A10x fusion processor. On the target processor, the existing reference PIPO code has performance of 34.6 cpb and 44.7 cpb in 64/128 and 64/256 standards. Among the proposed methods, the general implementation has a performance of 12.0 cpb and 15.6 cpb in the 8-plaintexts 64/128 and 64/256 standards, and 6.3 cpb and 8.1 cpb in the 16-plaintexts 64/128 and 64/256 standards. Compared to the existing reference code implementation, the 8-plaintexts parallel implementation for each standard has about 65.3%, 66.4%, and the 16-plaintexts parallel implementation, about 81.8%, and 82.1% better performance. The register minimum alignment implementation shows performance of 8.2 cpb and 10.2 cpb in the 8-plaintexts 64/128 and 64/256 specifications, and 3.9 cpb and 4.8 cpb in the 16-plaintexts 64/128 and 64/256 specifications. Compared to the existing reference code implementation, the 8-plaintexts parallel implementation has improved performance by about 76.3% and 77.2%, and the 16-plaintext parallel implementation is about 88.7% and 89.3% higher for each standard.

• Molecules and Cells
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• v.43 no.11
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• pp.909-920
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• 2020

Cytosolic Ca²⁺ levels ([Ca²⁺]_c) change dynamically in response to inducers, repressors, and physiological conditions, and aberrant [Ca²⁺]_c concentration regulation is associated with cancer, heart failure, and diabetes. Therefore, [Ca²⁺]_c is considered as a good indicator of physiological and pathological cellular responses, and is a crucial biomarker for drug discovery. A genetically encoded calcium indicator (GECI) was recently developed to measure [Ca²⁺]_c in single cells and animal models. GECI have some advantages over chemically synthesized indicators, although they also have some drawbacks such as poor signal-to-noise ratio (SNR), low positive signal, delayed response, artifactual responses due to protein overexpression, and expensive detection equipment. Here, we developed an indicator based on interactions between Ca²⁺-loaded calmodulin and target proteins, and generated an innovative GECI sensor using split nano-luciferase (Nluc) fragments to detect changes in [Ca²⁺]_c. Stimulation-dependent luciferase activities were optimized by combining large and small subunits of Nluc binary technology (NanoBiT, LgBiT:SmBiT) fusion proteins and regulating the receptor expression levels. We constructed the binary [Ca²⁺]_c sensors using a multicistronic expression system in a single vector linked via the internal ribosome entry site (IRES), and examined the detection efficiencies. Promoter optimization studies indicated that promoter-dependent protein expression levels were crucial to optimize SNR and sensitivity. This novel [Ca²⁺]_c assay has high SNR and sensitivity, is easy to use, suitable for high-throughput assays, and may be useful to detect [Ca²⁺]_c in single cells and animal models.