• Title/Summary/Keyword: Target Fusion

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Application of hybrid LRR technique to protein crystallization

  • Jin, Mi-Sun;Lee, Jie-Oh
    • BMB Reports
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    • v.41 no.5
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    • pp.353-357
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    • 2008
  • LRR family proteins play important roles in a variety of physiological processes. To facilitate their production and crystallization, we have invented a novel method termed "Hybrid LRR Technique". Using this technique, the first crystal structures of three TLR family proteins could be determined. In this review, design principles and application of the technique to protein crystallization will be summarized. For crystallization of TLRs, hagfish VLR receptors were chosen as the fusion partners and the TLR and the VLR fragments were fused at the conserved LxxLxLxxN motif to minimize local structural incompatibility. TLR-VLR hybridization did not disturb structures and functions of the target TLR proteins. The Hybrid LRR Technique is a general technique that can be applied to structural studies of other LRR proteins. It may also have broader application in biochemical and medical application of LRR proteins by modifying them without compromising their structural integrity.

A Study on the Microstructures and Electromagnetic Properties of Al-Co/AlN-Co Thin Films

  • Han, Chang-Suk;Han, Seung-Oh
    • Journal of the Korean Society for Heat Treatment
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    • v.24 no.1
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    • pp.16-22
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    • 2011
  • Al-Co/AlN-Co multilayer films with different layer thicknesses were prepared by using a two-facing target type D.C sputtering (TFTS) system. The deposited films were annealed isothermally at different temperatures and their microstructure, magnetic properties and resistivity were investigated. The magnetization of as-deposited films is very small irrespective of layer thickness. It was found that annealing conditions and layer thickness ratio (LTR) of Al-Co to AlN-Co can control the microstructure as well as the physical properties of the prepared films. The resistivity and magnetization increase and the coercivity decreases with decreasing LTR. High resistivity and sufficient magnetization were obtained for the films with LTR = 0.35. Films having such considerable magnetization and resistivity will be a potential candidate to be used for a high density recording material.

A Study on the Learning Method for Induction Motor Trajectory using a Neuro-Fuzzy Networks (뉴로-퍼지 네트워크에 의한 유도전동기 궤적의 학습에 관한 연구)

  • Yang, Seung-Ho;Kim, Sei-Chan;Kim, Duk-Hun;Yoo, Dong-Wook;Won, Chung-Yuen
    • Proceedings of the KIEE Conference
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    • 1994.07a
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    • pp.331-333
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    • 1994
  • A learning method for induction motor trajectory using neuro-fuzzy networks (NFN) based on fusion of fuzzy logic theory and neural networks is proposed. The premise and consequent parameters of the NFN affecting the controllers performances are modified during the learning stages by the proposed learning method to implement an optimal controller only with pre-determined target trajectory and the least amount of knowledge about an induction motor. The induction motor position control system is simulated to verify the effectiveness of the learned NF controller(NFC). The simulation results shows that the proposed learning method has good dynamic performance and small steady state error.

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Input Estimation in Multi-Sensor Environment (다중 감지기 시스템 하에서의 입력 추정 필터 구현)

  • Park, Yong-H.;Hwang, Ik-H.;Yoon, Jang-H.;Seo, Jin-H.
    • Proceedings of the KIEE Conference
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    • 1995.07b
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    • pp.699-701
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    • 1995
  • An input estimation technique is derived in multi-sensor environment. The proposed approach distribute the computational burden of input estimation to each local sensor and fusion center without loss of its optimality. The performances of proposed method in 2-sensor system are compared with those in single sensor system. Simulation results show that a reliable maneuvering target tracking system can be constructed in multi-sensor environment via proposed approach.

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Statistical Analysis of the Performance Reliability Data for Stroke Patients (뇌졸중 환자 운동신뢰성 측정치의 통계적 분석)

  • Byun, Jae-Hyun;Lee, Seung-Mi
    • Journal of Applied Reliability
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    • v.16 no.1
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    • pp.64-70
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    • 2016
  • Purpose: The purpose of this study was to evaluate performance reliability of stroke patients using kinematic analysis. Methods: A protocol to evaluate performance reliability was performed for three tasks on 20 stroke patients and 10 normal people. The tasks include hand to head (HH) task, hand to mouth (HM) task, and hand to target (HT) task. Results: The affected arms showed smaller joint angle, slower peak velocity, longer time to peak velocity for task performances than control group. Also, slower peak velocity and longer movement time for task performance in unaffected arm of stroke patients were obtained compared with the control group. Conclusion: Kinematic analysis is very useful quantitative tool to provide understanding on upper extremity function of stroke patients.

Preparation of Dopamine Transporter-specific Antibodies Using Molecular Cloned Genes

  • Lee, Shee-Yong;Im, Suhn-Young;Kim, Kyeong-Man
    • Archives of Pharmacal Research
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    • v.22 no.3
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    • pp.262-266
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    • 1999
  • Dopamine transporter (DAT) plays the most important role in terminating the actions of dopamines released into the synaptic cleft. DAT is also the target of various psychotropic drugs such as cocaine and amphetamine. In this study were prepared DAT-specific antibodies using the 2nd extracellular loop of rat DAT as an antigen. The 2nd extracellular loop of the rat DAT was expressed in bacterial as a fusion protein with glutathione-S-transferase, and injected ito rabbits to raise antibodies. Produced antibodies clearly recognized the rat DAT in ELISA, immunoblotting, and immumoprecipitation. As expected from the high sequence homology between the rat and human DAT, the antibodies raised for the rat DAT cross-reacted with the human DAT in the immunoblotting. Considering the specificity for DAT with wide range of applications such as ELISA, immunoblotting, and immunoprecipitation, these antibodies would be valuable tool for understanding the pharmacological actions of dopamine transporter and drug addition.

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A Method for Data Fusion of Multiple Target Information (다중 표적정보의 융합처리 방안)

  • Ryu, Chul-Hyung;Baek, Joo-Hyun;Jang, Won-Bum;Choi, Joon-Sung
    • Proceedings of the Korea Information Processing Society Conference
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    • 2012.11a
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    • pp.1200-1202
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    • 2012
  • 미래 전장에서 다수의 지상로봇들이 획득한 표적정보는 원격에 위치한 통제장치로 실시간 전송된다. 통제장치는 정찰영상과 디지털 지도위에 지상로봇들의 현재 동작 상태를 표시한다. 본 논문은 다수의 지상로봇들로부터 동일 표적에 대한 위치 정보가 수신될 경우 이에 대한 융합처리 방안을 제안한다. 이러한 융합처리를 통해 표적위치 정확도를 개선할 수 있으며, 이동 경로 추정을 통해 표적의 초기 탐지시간을 단축할 수 있다.

A Study on the Fabrication and Structural Properties of Al-Co/AlN-Co Thin Films

  • Han, Chang-Suk;Han, Seung-Oh
    • Korean Journal of Metals and Materials
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    • v.49 no.3
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    • pp.256-263
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    • 2011
  • We have synthesized Al-Co/AlN-Co multilayer films with different layer thicknesses by using a Two-Facing Target Type dc Sputtering (TFTS) system. The deposited films were annealed isothermally at different temperatures and their microstructure, magnetic properties and resistivity were investigated. The magnetization of the as-deposited films was found to be very small, irrespective of layer thickness. It was found that the annealing conditions and layer thickness ratio of Al-Co to AlN-Co (LTR) were able to control the microstructure, as well as the physical properties of the prepared films. The resistivity and magnetization increased and the coercivity decreased with a decreasing LTR. A high resistivity of 2500 ${\mu}{\Omega}-cm$, a magnetization of 360 $emu/cm^3$, and a coercivity of 5 Oe were obtained for the films with LTR=0.175.

Multiple Properties-Based Moving Object Detection Algorithm

  • Zhou, Changjian;Xing, Jinge;Liu, Haibo
    • Journal of Information Processing Systems
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    • v.17 no.1
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    • pp.124-135
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    • 2021
  • Object detection is a fundamental yet challenging task in computer vision that plays an important role in object recognition, tracking, scene analysis and understanding. This paper aims to propose a multiproperty fusion algorithm for moving object detection. First, we build a scale-invariant feature transform (SIFT) vector field and analyze vectors in the SIFT vector field to divide vectors in the SIFT vector field into different classes. Second, the distance of each class is calculated by dispersion analysis. Next, the target and contour can be extracted, and then we segment the different images, reversal process and carry on morphological processing, the moving objects can be detected. The experimental results have good stability, accuracy and efficiency.

A study for system design that guarantees the integrity of computer files based on blockchain and checksum

  • Kim, Minyoung
    • International Journal of Advanced Culture Technology
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    • v.9 no.4
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    • pp.392-401
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    • 2021
  • When a data file is shared through various methods on the Internet, the data file may be damaged in various cases. To prevent this, some websites provide the checksum value of the download target file in text data type. The checksum value provided in this way is then compared with the checksum value of the downloaded file and the published checksum value. If they are the same, the file is regarded as the same. However, the checksum value provided in text form is easily tampered with by an attacker. Because of this, if the correct checksum cannot be verified, the reliability and integrity of the data file cannot be ensured. In this paper, a checksum value is generated to ensure the integrity and reliability of a data file, and this value and related file information are stored in the blockchain. After that, we will introduce the research contents for designing and implementing a system that provides a function to share the checksum value stored in the block chain and compare it with other people's files.