• Journal of The Korean Society of Grassland and Forage Science
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• v.37 no.3
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• pp.223-230
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• 2017

Arabidopsis nucleoside diphosphate kinase 2 (AtNDPK2) is an upstream signaling molecule that has been shown to induce stress tolerance in plants. In this study, the AtNDPK2 gene, under the control of a stress-inducible SWPA2 promoter, was introduced into the genome of tall fescue (Festuca arundinacea Schreb.) plants. The induction of the transgene expression mediated by methyl viologen (MV) and NaCl treatments were confirmed by RT-PCR and northern blot analysis, respectively. Under salt stress treatment, the transgenic tall fescue plants (SN) exhibited lower level of $H_2O_2$ and lipid peroxidation accumulations than the non-transgenic (NT) plants. The transgenic tall fescue plants also showed higher level of NDPK enzyme activity compared to NT plants. The SN plants were survived at 300 mM NaCl treatment, whereas the NT plants were severely affected. These results indicate that stress-inducible overexpression of AtNDPK2 might efficiently confer the salt stress tolerance in tall fescue plants.

• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.2
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• pp.101-108
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• 2007

The object of this experiment was to investigate the effect of mixture which consist of different seeding rates and species on dry matter yield and quality in Daejon area. The field trials were conducted from 2003 to 2005 at Chungnam National University in order to evaluate the dry matter yield and quality of different mixture. The experimental design includes three mixture types: Conventional mixtures, CM{orchardgrass(Potomac) 50% + tall fescue (Fawn)20% + Kentucky bluegrass(Kenblue) 20% + white clover(Regal) 10%}, red clover + mixtures, RM{orchardgrass(Potomac) 40% + tall fescue(Fawn) 20% + Kentucky bluegrass(Kenblue) 10% + red clover(Kenland) 30%) and Turf type grass + mixtures, TM {orchardgrass (Potomac) 50% + turf type grass (tall fescue, Millennium 20% + Kentucky bluegrass, Midnight 10% + perennial ryegrass, Palmer III 10%) + white clover(Regal) 10%}. The average DM yield for 2 years of red clover+mixture(11,656kg/ha) was higher than those of the other mixtures(p<0.05). The content of crude protein and dry manu digestibility were higher in red clover+mixture than in other mixtures(p<0.05). but, the content of fibrous compounds like as NDF, ADF, cellulose and lignin were lower in red clover+mixture than in other mixtures(p<0.05). The crude protein dry matter(CPDM) yield was higher in red clover + mixture(2,832kg/ha) than in conventional mixture(2,372kg/ha) and turf type + mixture(2,266kg/ha)(p<0.05). The digestible dry matter (DDM) yield was higher in red clover + mixture(8,881 kg/ha) than in conventional mixture(8,255kg/ha) and turf type + mixture(7,314kg/ha)(p<0.05). In botanical composition at last cutting time in 2005, conventional mixture were maintained orchardgrass 45%, tall fescue 22%, Kentucky bluegrass 5% and white clover 24%. Red clover + mixture were maintained orchardgrass 40%, tall fescue 22%, Kentucky bluegrass 4% and red clover 31%. turf type + mixture were orchardgrass 37%, tall fescue 23%, Kentucky bluegrass 6%, perennial ryegrass 8% and white clover 24%, respectively. As summary, DM yields and quality of mixture species and seeding rates were observed significant difference. The results of this experiment indicated that red clover + mixture was more effective in enhancing the DM yield and forage quality in Daejon area.

• Asian Journal of Turfgrass Science
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• v.16 no.1
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• pp.1-10
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• 2002

The influence of calcium on the growth of creeping bentgrass 'Penn-Al', perennial ryegrass 'Palmer II', Kentuckuy bluegrass 'Nassou' and tall fescue 'Boonsai 2000' in greenhouse was investigated. Creeping bentgrass 'Penn-A1', Kentucky bluegrass 'Nassou' and tall fescue 'Boonsai 2000' at Ca 4.0 me/L, and perennial ryegrass 'Palmer II' at Ca 2.0 me/L showed the best shoot growth. Creeping bentgrass 'Penn-A1', perennial ryegrass 'Palmer II' and Kentucky bluegrass 'Nassou' at Ca 1.0 me/L, and tall fescue 'Boonsai 2000' at Ca 4.0 me/L showed the best root growth, and there was little or no difference between different Ca concentrations. Creeping bentgrass 'Penn-Al' and Kentucky bluegrass 'Nassou' at Ca 4.0 mea, and perennial ryegrass 'Palmer II' at Ca 1.0 me/L had the highest number of tillers, and tall fescue 'Boonsai 2000' at Ca 4.0 me/L had the highest, but there was no difference between different Ca concentrations. As application rate of Ca concentration became higher, the concent of Ca in plant tissue increased, while the content of Mg in plant tissue decreased, and the content of Fe in plant tissue increased to Ca 4.0 me/L. The Ca treatment had not effect on N, p, K, and Zn of tissue content. The wick pot applied will be to research of plant nutrition in future because utilization of wick pot has an excellent precision and convenience.

• Journal of The Korean Society of Grassland and Forage Science
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• v.20 no.1
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• pp.61-66
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• 2000

An experiment was conducted to evaluate the effects of seed coating on germination and early growth of tall fescue (Festuca arundinacea Schr.), orchardgrass (Dactylis glomerata L.), and alfalfa (Medicago sativa L.) over sown on unploughed hilly and forested areas. Establishment and early growth of coated tall fescue and orchardgrass on unploughed hilly area were not significantly influenced by seed coating. Establishment and early growth of coated alfalfa on unploughed hilly area were higher than non-coated and showed 4.7% and 4.0 g/m2, respectively. Establishment of coated tall fescue (11.8%) on forested area were higher than noncoated (0.3%), but ealy growth of tall fescue was not significantly influences by seed coating. Establishment of over sown orchardgrass on forested area was not significantly affected by seed coating, but early growth of coated seed (18.7%) was better than that of non-coated (0.3%). Establishment of coated alfalfa on forested area were higher than those of non-coated. Results of this study indicate that the establishment and early growth of over sown tall fescue, orchardgrass, and alfalfa could be improved by seed coating.

• Journal of The Korean Society of Grassland and Forage Science
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• v.29 no.4
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• pp.299-306
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• 2009

Oxidative stress is the main limiting factor in crop productivity. To solve global environmental problems using the plant biotechnology, we have developed on the oxidative stress-tolerant transgenic tall fescue plants via Agrobacterium-mediated genetic transformation method. In order to develop transgenic tall fescue (Festuca arundinacea Schreb.) plants with enhanced tolerance to multiple environmental stresses, nucleotide diphosphate kinase gene under the control of CaMV35S promoter were introduced into genome of tall fescue plants. Proteomic analysis revealed that transgenic tall fescue not only accumulated NDP kinase 2 protein in their cells, but also induced several other antioxindative enzyme-related proteins. When leaf discs of transgenic plants were subjected to cold stress, they showed approximately 30% less damage than wild-type plants. In addition, transgenic tall fescue plants showed normal growth when transgenic plants were subjected to $4^{\circ}C$ for 3 days treatments. These results suggest that transgene is important in ROS scavenging by induction of antioxidative proteins, and could improve abiotic stress tolerance in transgenic tall fescue plants.

• Journal of The Korean Society of Grassland and Forage Science
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• v.24 no.2
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• pp.155-162
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• 2004

Different adhesives(CF-clear, arabic gum, cethylmethyl cellulose, polyvinyl alcohol, polyvinyl pyrrolidone, hydroxy propyl cellulose, methyl cellulose) and particulate matters(bentonite, kaolin, calcium carbonate, peatmoss, talcum, vermiculite, zeolite, calcium hydroxide, plaster, calcium phosphate, vermiculite + calcium carbonate, vermiculite + kaolin, vermiculite + talcum) were evaluated by coating index method. According to the weight of single coated seeds, CF-clear and polyvinyl alcohol appeared to be the best coating adhesives for red clover and tall fescue seeds. Both vermiculite and vermiculite + talc(l : 1) for red clover, and vermiculite + talc(l : 1) for tall fescue appeared to be the best particulate matter(P < 0.01). Percent friability showed excellent results for the arabic gum, CF-clear, and polyvinyl alcohol when compared to others(p < 0.01). The coating index showed high with vermiculite and vermiculite + talc(l : 1) in coating red clover seed, and talc, vermiculite, and vermiculite + talc showed good coating index in coating tall fescue seed.

• Journal of Animal Science and Technology
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• v.46 no.4
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• pp.701-706
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• 2004

The objective of this experiment was to compare the dry matter intake, nutrients digestibility and nitrogen and energy utilization of herbages harvested from turf type mixtures {tall fescue(Rebell Jr.) 40%+ perennial ryegrass(Palmer II) 20%+ Kentucky bluegrass(Newport) 10%+ redtop(Banicuda) 10%+ red fescue(Salem) 10%+ creeping bentgrass(Crenshaw) 10%} and tall type mixtures {orchardgrass(Potomac) 40%+ tall fescue(Fawn) 20%+ Festuloium braunii(Paulita) 10%+ perennial ryegrass(Reveille) 10%+ timothy(Climax) 10%+ red clover(Kenblue) 10%+ alfalfa(Vernal) 10%} by Korean native goats. This experiment was conducted by total collection method in laboratory, 2001. The voluntary DM intake per body weight were slightly higher for turf type mixtures(28.9g) than that of tall type mixtures(26.2g), but there was no significant difference. The digestibility of cellular constituents was slightly higher for turf type mixtures than that of tall type mixtures(p<0.05), but digestibility of DM, NDF and ADF did not differ between two mixtures. The retained nitrogen percent(apparently biological value) was slightly higher for turf type mixtures(56.7%) than that of tall type mixtures(56.4%), but there was no significant difference between two mixtures. Apparently retained digestible energy was slightly higher for turf type mixtures(58.3%) than that of tall type mixtures(57.9%), but there was no significant difference between two mixtures. Based on the results, the herbage utilization by Korean native goats did not showed any significant difference between two mixtures, therefore there is a possibility that herbages of turf type mixtures can be used as a roughages by livestock.

• Journal of The Korean Society of Grassland and Forage Science
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• v.16 no.2
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• pp.127-132
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• 1996

This study was carried out to obtain good livestock production in comparison with 3 different pasture mixture (Control : TI = Potomac orchardgrass + Bastion perennial ryegrass + Fawn tall fescue + Regal white clover, T2 = Potomac orchardgrass + Bastion perennial ryegrass + Roa tall fescue + Regal white clover, T3 = Potomac orchardgrass + Bastion perennial ryegrass + Roa tall fescue + Tahora white clover) by grazing experiment used 45 dairy calves (about lOOkg liveweight) during the period from April to October, 1994 at the Isidole farm, Cheju. Daily liveweight gains of calves grazed during the experiment period were 331${\pm}$29, 352${\pm}$4, 356${\pm}$18g in treatment 1, 2 and 3 respectively, but did not get statistically significant difference. On compared the results with the period investigated, the period which was highest in daily liveweight gain was early grazing season b m May to June while the period shown to be lowest in daily liveweight gain was between late July and late August. Hehage intake rate did not get significant difference among treatments with results shown as 67${\pm}$1.2, 62${\pm}$5.1, 60${\pm}$9.6% in treatment 1, 2 and 3 respectively. And efficiency of conversation into animal product was high in treatment 2. It is considered that good results were not obtained in this study because tall fescue and white clover, important species in this trial did not cover the pasture well with slow establishment in 1st year after seeding.

• Asian Journal of Turfgrass Science
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• v.13 no.2
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• pp.71-78
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• 1999

The influence of increasing livel (0.0, 0.05, 0.1, 0.2, 0.4, 0.6, 0.8, 1.2, 1.6, and 2.0%) fo NaCl on the germination of red fescue (Festuca rubra) 'Sea Breeze', tall fesce(Festucaarundinacea) 'Pixie', creeping red fescue (Festuca rubra ssp. rubra) 'Cindy', annual rye-grass (Lolium multiflorum) 'Permer Ⅱ', perennial ryegrass (Lolium perenne) 'Pennant', fairway wheatgrass(Agropyron cristatum), creeping bentgrass (Agrostis palustris) 'Penncross', and kentucky bluegrass (Poa pratensis) 'Nuglade' was investigated. Red fescue 'Sea Breeze', tall fescue 'Pixie', and creeping red fescue 'Cindy' had greater than 90% seed germination at NaCl concentrations of 0.2% or lower, while showing similar seeding shoot and root lengths and TAA50 values as the control. Creeping red fescue 'Cindy'gave at 0.6% or higher NaCl. Perennial ryegrass 'Pennant' and annual ryegrass 'Permer Ⅱ' showed more than 95% seed germination when NaCl concentrations were 0.4% and 0.8%, respectively. Fairway wheatgrass, creeping bentgrass 'Penncross' and Kentucky bluegrass 'Nugade' had showing similar germination percent, shoot and root lengths and T50 values as the control at NaCl concentrations of 0.1% or lower. In general, germination percent and the lengthes of seedling roots and shoots of all species tested decreased as NaCl concentrations iscreased. The T50 values became greater as NaCl concentration increased. Seed fermination in red fescue 'Sea Breeze', tall fescue 'Pixie',perennial ryegrass 'Pennant', and annual ryegrass 'Permer Ⅱ' was compoetely inhibited at 2.0% NaCl. Creeping red fescue 'Cindy' and fairway wheatgrass gardly germinated at 1.6% MaCl. Creeping bentgrass 'Penncross' and Dentucky bluegrass 'Nuglade' showed a complete inhibition of germination at 1.2% and 0.6% NaCl, respectively.

• Journal of Animal Science and Technology
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• v.46 no.3
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• pp.443-450
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• 2004

To examine the potentiality of grassland dominated with turf-type grasses, which are mainly used for the establishment of green spaces, forage production and its utilization as forage resources, dry matter yield and forage quality of three different turf-type mixtures were estimated throughout three consecutive experimental years. According to the experiment, the mean of dry matter(DM) yield, chemical composition, DM digestibility, crude protein dry matter(CPDM) and digestible dry matter(DDM) yield was not significantly different among species(p > 0.05) over three experimental years. Botanical composition of three dominant species(tall fescue, Kentucky bluegrass and perennial ryegrass) was 42, 35 and 28%, respectively, in three mixtures at the last cutting(6th) in 2001. The botanical composition of three dominated species such as tall fescue, Kentucky bluegrass and perennial ryegrass was maintained 84% of the total plants in all types of mixtures. Therefore, It is observed that the mixture types did not closely affect the botanical composition. But botanical composition of the other species such as redtop(8%) and creeping bentgrass(6${\sim}$7%) was low. Especially, that of red fescue was the least(1${\sim}$2%) of all mixed species. As mentioned above, any difference was not observed in DM yields, forage quality and botanical composition among mixture types. Therefore, any types of mixtures can be used as public green spaces like school ground areas or river-sides, and as forage resources additionally.