• 대한본초학회지
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• 제25권3호
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• pp.97-101
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• 2010

Objectives : The present study investigated the anti-nociceptive and anti-inflammatory properties of the water extract of Geranii Herba (The stem and leaves of Geranium thunbergii Sieb. et Zucc.) in the animal models of pain and inflammation. Methods : We evaluated the anti-nociceptive and anti-inflammatory activities of Geranii Herba extract (GHE) using the writhing test, tail-flick test, carrageenan-induced paw edema and xylene-induced ear edema models. Two dose of GHE (100 and 1000 mg/kg) was administrated orally to the mice. Control group received normal saline and ibuprofen (50 mg/kg) was used as a positive control drug. Results : GHE 1000 mg/kg treated group showed an increased tail-flick response time in the tail-flick test and inhibitory effect on writhing syndrome induced by acetic acid. Treatment with GHE at the same dose inhibited ear edema induced by xylene and foot edema induced by carrageenan toxicity. Conclusion : The results demonstrate that GHE has anti-nociceptive and anti-inflammatory effects in the various models of nociception and inflammation.

• Nutrition Research and Practice
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• 제3권2호
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• pp.114-121
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• 2009

The effect of diet supplemented with red beet (Beta vulgaris L.) leaf on antioxidant status of plasma and tissue was investigated in C57BL/6J mice. The mice were randomly divided into two groups after one-week acclimation, and fed a high fat (20%) and high cholesterol (1%) diet without (control group) or with 8% freeze-dried red beet leaf (RBL group) for 4 weeks. In RBL mice, lipid peroxidation determined as 2-thiobarbituric acid-reactive substances (TBARS value) was significantly reduced in the plasma and selected organs (liver, heart, and kidney). Levels of antioxidants (glutathione and $\beta$-carotene) and the activities of antioxidant enzyme (glutathione peroxidase) in plasma and liver were considerably increased, suggesting that antioxidant defenses were improved by RBL diet. Comet parameters such as tail DNA (%), tail extent moment, olive tail moment and tail length were significantly reduced by 25.1%, 49.4%, 35.4%, and 23.7%, respectively, in plasma lymphocyte DNA of RBL mice compared with control mice, and indicated the increased resistance of lymphocyte DNA to oxidative damage. In addition, the RBL diet controlled body weight together with a significant reduction of fat pad (retroperitoneal, epididymal, inguinal fat, and total fat). Therefore, the present study suggested that the supplementation of 8% red beet leaf in high fat high cholesterol diet could prevent lipid peroxidation and improve antioxidant defense system in the plasma and tissue of C57BL/6J mice.

• Journal of Ginseng Research
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• 제21권2호
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• pp.109-114
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• 1997

The effect of total saponin fraction of Ginseng injected intrathecally (i.1.) or in- tracerebroventricularly (i.c.v.) on the antinociception induced by D-$Pen^{2,5}$- enkephalin (DPDPE) ad ministered i.c.v. was studied in ICR mice in the present study. The antinociception was assessed by the tail-flick test. Total saponin fraction at doses 0.1 to 1.0 $\mu\textrm{g}$, which administered i.t. Alone did not affect the latencies of tail-flick threshold, attenuated dose-dependently the inhibition of the tail-flick response induced by i.c.v. administered DPDPE (10 $\mu\textrm{g}$). However, total saponin fraction at doses 1 to 20 $\mu\textrm{g}$, which administered i.c.v. Alone did not affect the latencies of the tail-flick response, did not affect i.c.v. administered DPDPE (10 $\mu\textrm{g}$)-induced antinociception. The duration of antagonistic action of total saponin fraction against DPDPE-induced antlnociception was lasted at least for 6 hrs. Various doses of ginsenosides Rd, but not $\Rb_2$, Rc, Rg1, and $\Rb_1$ and Re, injected i.t. Dose-dependently attenuated antinociception induced by DPDPE administered i.c.v. Our results indicate that total saponin fraction injected spinally appears to have antagonistic action against the antinociception induced by supraspinally applied DPDPE. Ginsenoside Rd appears to be responsible for blocking j.c.v. administered DPDPE-induced antinociception. On the other hand, total ginseng fraction, at supraspinal sites, may not have an antagonistic action against the antinociception induced by DPDPE.

• 조명전기설비학회논문지
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• 제19권1호
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• pp.109-116
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• 2005

풍력 및 태양광 발전시스템은 화석에너지의 고갈에 대한 대체에너지로 각광을 받고 있으며, 환경오염을 발생하지 않고 무한정으로 사용할 수 있으나 풍속과 태양광의 변화에 따른 안정성의 문제가 발생한다. 풍력발전시스템의 경우 태풍과 급속한 풍속의 변화에 의해 시스템의 안정성 문제가 발생한다. 본 논문에서는 풍속을 이용한 피드백 제어를 기초로 하는 자동강풍제어기를 포함하는 풍력 발전시스템을 구성하였으며, 이를 다양한 조건의 실험을 통하여 입증하였다. 태양전지 어레이의 최대 출력을 위한 MPPT제어와 고르지 못한 DC 전압을 정류하기 위하여 buck-boost컨버터를 사용하였고, 실험을 동하여 시스템 출력전류 리플 저감의 결과를 확인하였다.

• Journal of Preventive Medicine and Public Health
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• 제37권4호
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• pp.373-380
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• 2004

Objectives : There has been gradually increasing concern about the adverse health effects of electromagnetic radiation originating from cell phones which are widely used in modern life. Cell phone radiation may affect human health by increasing free radicals of human blood cells. This study has been designed to identify DNA damage of blood cells by electromagnetic radiation caused by cell phone use. Methods : This study investigated the health effect of acute exposure to commercially available cell phones on certain parameters such as an indicator of DNA damage for 14 healthy adult volunteers. Each volunteer during the experiment talked over the cell phone with the keypad facing the right side of the face for 4 hours. The single cell gel electrophoresis assay (Comet assay), which is very sensitive in detecting the presence of DNA strand-breaks and alkali-labile damage in individual cells, was used to assess peripheral blood cells (T-cells, B-cells, granulocytes) from volunteers before and after exposure to cell phone radiation. The parameters of Comet assay measured were Olive Tail Moment and Tail DNA %. Results : The Olive Tail Moment of B-cells and granulocytes and Tail DNA % of B-cells and granulocytes were increased by a statistically significant extent after 4-hour use of a cell phone compared with controls. Conclusion : It is concluded that cell phone radiation caused the DNA damage during the 4 hours of experimental condition. Nonetheless, this study suggested that cell phone use may increase DNA damage by electromagnetic radiation and other contributing factors.

• 한국음향학회지
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• 제25권7호
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• pp.312-318
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• 2006

기존의 립싱크 시스템은 음소 분할 후, 각각의 음소를 인식하는 2단계의 과정을 거쳤다. 하지만, 정확한 음소 분할의 부재와 음성이 끊긴 분할 된 음소로 이루어진 훈련 데이터들은 시스템의 전체 성능을 크게 떨어뜨렸다. 이런 문제를 해결하기 위해 Head-Body-Tail (HBT) 모델을 이용한 단모음 연속어 인식 기술을 제안한다. 주로 소규모 어휘를 다루는데 적합한 HBT 모델은 Head 와 Tail 부분에 문맥 종속 정보를 포함하여 앞 뒤 문맥에 따른 조음효과를 최대한 반영한다. 또한, 7개의 단모음을 입모양이 비슷한 세 개의 클래스로 분류하여, 클래스에 종속적인 코드북 3개를 가진 반연속HMM (Hidden Markov Model)을 적용하여 시스템을 최적화하고, 변이 부분이 큰 단어의 처음과 끝은 연속HMM의 8 믹스쳐 가우시안 구조를 사용하여 모델링하였다. 제안한 방법은 HBT구조의 연속HW과 대등한 성능을 보이지만, 파라미터 수는 33.92% 감소하였다. 파라미터 감소는 계산 양을 줄여주므로, 시스템이 실시간으로 동작 가능하게 한다.

• Journal of Acupuncture Research
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• 제20권3호
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• pp.117-130
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• 2003

To study the analgesic and effect and its mechanism of eletroacupunture(EA) on the chronic inflammatory pain 50 rats were induced with arthralgesia by injecting complete freund's adjuvant(CFA). Two weeks after the injection of CFA, EA stimulation(2Hz, 0.07mA, 0.3ms) was delivered to Jogsamni($ST_{36}$) for 20 minutes. Analgesic effect was evaluated by using the tail flick latency(TFL) and the analgesic mechanism was observed by applying TFL with the pretreatment with naloxone and yohimbine. The results were as follows ; 1. TFL level for the model of adjuvant-induced arthritis decreased as time went by and it induced the hyperalgesia. 2. EA stimulation delivered to Jogsamni($ST_{36}$) for 20 minutes in the rat model of adjuvant-induced arthritis brought analgesic effect and its effect had lasted for 40 minutes after the stimulation. 3. The analgesic effect of Jogsamni($ST_{36}$) EA in the rat model of adjuvant-induced arthritis was blocked by pretreatment with naloxone(2mg/kg,i.p). This result suggests that the EA effect on the chronic inflammatory pain can be related to the endogenous opioid mechanism. 4. The analgesic effect of Jogsamni($ST_{36}$) EA in the rat model of adjuvant-induced arthritis was blocked by pretreatment with naloxone(2mg/kg,i.p). This result suggests that the EA effect on the chronic inflammatory pain can be related to the ${\alpha}_2$-adrenergic mechanism.

• Molecular & Cellular Toxicology
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• 제5권2호
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• pp.126-132
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• 2009

The usage and types of chemicals are advancing, specializing, large-scaled increasing, and new chemical exposed workers are concerning to occupational disease. The generation of reactive oxygen in the body from carcinogen, mutation and DNA damage in cancer is protected by natural antioxidants (phytochemicals) with antimutagenic effect. There were many reports of ginsenoside Rb$_1$, Rg$_1$ grievances of the genetic mutation to suppress the effect confirm the genetic toxicity test with chromosomal aberration test and the Comet (SCGE) assay confirmed the suppression effect occurring chromosomal DNA damage. We had wanted to evaluate the compatibility and sensitivity between the chromosomal aberration (CA) test and the Comet assay. We used the CA test and Comet assay to evaluate the anti-genotoxicity of ginsenoside Rb$_1$ and Rg$_1$, in CHO-K1 (Chinese hamster ovary fibroblast) cell in vitro, composed negative control (solvent), positive control (benzo[a]pyrene), test group (carcinogen+variety concentration of ginsenoside) group. The positive control was benzo[a]pyrene (50 $\mu$M), well-known carcinogen, and the negative control was the 1 % DMSO solvent. The test group was a variety concentration of ginsenoside Rb$_1$, Rg$_1$ with 10$^{-8}$%, 10$^{-6}$%, 10$^{-4}$%, 10$^{-2}$%, 1%, 10%. In chromo-somal aberration test, we measured the number of cells with abnormally structured chromosome. In Comet assay, the Olive tail moment (OTM) and Tail length (TL) values were measured. The ratio of cell proliferation was increased 8.3% in 10$^{-8}$%, 10$^{-6}$%, 10$^{-4}$%, 10$^{-2}$%, 1%, 10% Rb$_1$ treated groups, and increased 10.4% in 10$^{-10}$%, 10$^{-8}$%, 10$^{-6}$%, 10$^{-4}$%, 10$^{-2}$%, 1% Rg$_1$ treated groups. In the CA test, the number of chromosomal aberration was decreased all the Rb$_1$ and Rg$_1$ treated groups. In the Comet assay, the OTM values were decreased in all the Rb$_1$ and Rg$_1$ treated groups. To evaluate the compatibility between CA and Comet assay, we compared the reducing ratio of chromosomal abnormalities with its OTM values, it was identified the antimutagenicity of ginsenoside, but it was more sensitive the CA test than the Comet assay. Ginsenoside Rb$_1$ and Rg$_1$ significantly decrease the number of cells with chromosomal aberration, and decrease the extent of DNA migration. Therefore, ginsenoside Rb$_1$, Rg$_1$ are thought as an antioxidant phytochemicals to protect mutagenicity. The in vitro Comet assay seems to be less sensitive than the in vitro chromosomal aberration test.

• 한국약용작물학회지
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• 제11권2호
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• pp.89-96
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• 2003

기주목(상수리나무, 밤나무, 자작나무, 버드나무)별 겨우살이 ethanol추출물과 5가지 분획물 (hexane fr., chloroform fr., ethyl acetate fr., butanol fr., aqueous fr.)을 이용하여 in vitro에서 DPPH를 이용한 수소전자공여능, DNA에 대한 산화적 손상 및 손상억제 효과 등에 미치는 영향을 연구하기 위하여 comet assay를 실시하였다. 4종의 한국산 겨우살이의 ethanol추출물과 분획물 중 ethyl acetate fr.들이 높은 수소전자공여능을 보였다. Comet assay분석에서 head DNA의 형태를 유지하거나 또는 tail DNA의 생성을 억제하는 겨우살이 fr.들이 DNA에 대하여 항산화적 기능이 있음을 보여 주었다. 또는 comet assay 유용한 parameter인 $tail\;length({\mu}m)$는 positiye control인 $H_2O_2\;(10^{-3}M)$로 40min동안 세포에 처리하여 산화적 손상을 준 결과, $149{\mu}m$의 tail length를 보였으며 , 상수리나무 겨우살이의 ethyl acetate fr.(0.1mg/ml)을 $H_2O_2\;(10^{-3}M)$와 동시 처리에는 $110{\mu}m$, 자작나무 겨우살이 ethyl acetate fr.은 $104{\mu}m$, 상수리나무 겨우살이 butanol fr.은 $123{\mu}m$, 자작나무 겨우살이 butanol fr.은 $118{\mu}m$로 tail length가 감소하는 것으로 보아 겨우살이가 산화적 DNA손상을 방어한다는 것을 알 수 있었다. 이상의 결과로 볼 때 한국산 겨우살이는 산화적 stress를 일으키는 활성 산소 자유라디칼의 생성에 대해 보호활성을 나타내어 유용한 chemopreventive agent의 기능이 기대된다.

• 한국터널지하공간학회 논문집
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• 제19권2호
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• pp.265-281
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• 2017

얕은 터널의 시공에 있어 지표 침하는 주요 관리 사항으로 쉴드 TBM 기술을 적용함으로써 굴착 중 지반 변형의 제어를 통하여 침하를 경감시키는 것이 가능하며, 특히 뒤채움 주입은 침하 경감의 목적으로 쉴드 공법에서는 일반적으로 적용되는 기술이다. 투수성이 낮은 지반에서의 TBM 시공에 의한 지표 침하는 터널 시공 중에 발생할 뿐만 아니라, 터널 관통 후에도 장기간에 걸쳐 발생한다. 장기 침하는 주로 터널 주변의 압밀에 의해 발생되고, 이 압밀 과정은 터널 주변에 과잉간극수압을 유발하는 뒤채움 주입에 의해 영향을 받게 되며 결과적으로 쉴드 TBM 터널에서는 뒤채움 주입이 장기 침하에 큰 영향을 주게 된다. 본 연구에서는 쉴드 TBM 공법 중 뒤채움 주입이 지표 침하에 미치는 영향을 파악하기 위해 3차원 응력-간극수압 연계해석을 수행하였다. 해석 결과 뒤채움 주입압의 증가는 단기 침하를 경감시키지만, 다수의 경우에서 장기 침하의 감소에 기여를 하지 않는 것으로 나타났다. 또한, 장기 침하를 최소한으로 제한할 수 있는 한계 주입압의 존재를 확인하였다.