• 제목/요약/키워드: TX-100

검색결과 68건 처리시간 0.025초

Molecular Association of Glucose Transporter in the Plasma Membrane of Rat Adipocyte

  • Hah, Jong-Sik
    • The Korean Journal of Physiology
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    • 제25권2호
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    • pp.115-123
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    • 1991
  • Molecular association of glucose transporters with the other proteins in the plasma membrane was assessed by gel electrophoresis and immunoblot techniques. Approximately $31.5{\pm}5.1%$ of GLUT-4, $64.8{\pm}2.7%$ of clathrin, 48.7% of total protein in the plasma membrane (PM) were found insoluble upon extraction with 1% Tx-100. Sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed that the Tx-100 insoluble PM fraction contained about 4 major polypeptides with apparent molecular weight of above 200, 100-120, 80 and 30-35 KDa that were readily removed upon wash with a high pH buffer which is known to remove clathrin and 0.5 M Tris-buffer which is known to remove assembly proteins (AP). Immunoblotting of GLUT4 and clathrin against specific antibodies showed that GLUT-4 and clathrin were co-solubilized up to 84.6% and 82.7% respectively by wash with a high pH buffer and 1% Tx-100. When the membrane was pre-washed with a high pH buffer and 0.5 M Tris solution, GLUT4 and clathrin were not solubilized further suggesting that GLUT4 molecules are in molecular association with clathrin, AP and/or other extrinsic membrane proteins in plasma membrane and the formation of clathrin-coated structures might be involved in insulin stimulated glucose transporter translocation mechanism.

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너치와 아이솔레이션을 활용한 CDMA와 W-CDMA 듀얼밴드용 유전체 듀플렉셔와 밴드패스 필터의 설계 및 제작 (Design and Fabrication of Dielectric Duplexers and Bandpass Filters for CDMA and W-CDMA Dualband Using the Notch and Isolation)

  • 최우성;양성현
    • 한국정보통신학회논문지
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    • 제15권6호
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    • pp.1227-1232
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    • 2011
  • 너치와 아이솔레이션을 활용하여 제작한 CDMA와 WCDMA 듀얼밴드용 유전체 듀플렉셔와 밴드패스필터의 삽입손실과 이아솔레이션을 조사하였다. 측정한 최저 삽입손실과 분리도는 CDMA 밴드에서 -2.1708dB와 45dB, WCDMA 밴드에서 -2.6946dB와 100dB를 각각 보여주고 있다. 이러한 특성으로부터 제작된 듀플렉셔와 밴드패스 필터는 CDMA와 WCDMA 듀얼밴드에서 감쇠의 증가와 간섭을 감소시키는 역할을 할 것이다.

Micellar Catalysis on 1,10-Phenanthroline Promoted Chromic Acid Oxidation of Ethane-1,2-diol in Aqueous Media at Room Temperature

  • Ghosh, Sumanta K.;Saha, Rumpa;Ghosh, Aniruddha;Basu, Ankita;Mukherjee, Kakali;Saha, Indrajit;Saha, Bidyut
    • 대한화학회지
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    • 제56권6호
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    • pp.720-724
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    • 2012
  • Under pseudo-first order conditions, the monomeric species of Cr(VI) was found to be kinetically active in the absence of phenanthroline (phen) whereas in the phen-promoted path, the Cr(VI)-phen complex undergoes a nucleophilic attack by etane-1,2-diol to form a ternary complex which subsequently experience a redox decomposition leading to hydroxy ethanal and Cr(III)-phen complex. The effect of the cationic surfactant (CPC), anionic surfactant (SDS) and neutral surfactant (TX-100) on the unpromoted and phen-promoted path have been studied. Micellar effects have been explained by considering the preferential partitioning of reactants between the micellar and aqueous phase. Combination of TX-100 and phenanthroline will be the ideal for chromic acid oxidation of ethane-1,2-diol in aqueous media.

Treatment modalities for Korean patients with unilateral hemifacial microsomia according to Pruzansky-Kaban types and growth stages

  • Yang, Il-Hyung;Chung, Jee Hyeok;Yim, Sunjin;Cho, Il-Sik;Kim, Sukwha;Choi, Jin-Young;Lee, Jong-Ho;Kim, Myung-Jin;Baek, Seung-Hak
    • 대한치과교정학회지
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    • 제50권5호
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    • pp.336-345
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    • 2020
  • Objective: To investigate the treatment modalities (Tx-Mods) for patients with unilateral hemifacial microsomia (UHFM) according to Pruzansky-Kaban types and growth stages. Methods: The samples consisted of 82 Korean UHFM patients. Tx-Mods were defined as follows: Tx-Mod-1, growth observation due to mild facial asymmetry; Tx-Mod-2, unilateral functional appliance; Tx-Mod-3, fixed orthodontic treatment; Tx-Mod-4, growth observation due to a definite need for surgical intervention; Tx-Mod-5, unilateral mandibular or bimaxillary distraction osteogenesis (DO); Tx-Mod-6, maxillary fixation using LeFort I osteotomy and mandibular DO/sagittal split ramus osteotomy; Tx-Mod-7, orthognathic surgery; and Tx-Mod-8, costochondral grafting. The type and frequency of Tx-Mod, the number of patients who underwent surgical procedures, and the number of surgeries that each patient underwent, were investigated. Results: The degree of invasiveness and complexity of Tx-Mod increased, with an increase in treatment stage and Pruzansky-Kaban type (initial < final; [I, IIa] < [IIb, III], all p < 0.001). The percentage of patients who underwent surgical procedures increased up to 4.2 times, with an increase in the Pruzansky-Kaban type (I, 24.1%; IIa, 47.1%; IIb, 84.4%; III, 100%; p < 0.001). However, the mean number of surgical procedures that each patient underwent showed a tendency of increase according to the Pruzansky-Kaban types (I, n = 1.1; IIa, n = 1.5; IIb, n = 1.6; III, n = 2.3; p > 0.05). Conclusions: These findings might be used as basic guidelines for successful treatment planning and prognosis prediction in UHFM patients.

Cetylpyridinium Chloride와 Triton X-100의 혼합 미셀화에 미치는 염 및 부탄올 이성질체들의 효과 (Effect of Salts and Isomeric Butanols on the Mixed Micellar Properties of Cetylpyridinium Chloride with Triton X-100)

  • 정종재;이상학;김영철;이병환
    • 공업화학
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    • 제9권7호
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    • pp.968-973
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    • 1998
  • 양이온성 계면활성제인 cetylpyridinium chloride(CPC)와 비이온성 계면활성제인 Triton X-100(TX-100)의 혼합계면활성제에 염(KCl과 $Na_2CO_3$)과 부탄올 이성질체(tert-부탄올, iso-부탄올과 n-부탄올)를 첨가하였을 때 변화되는 임계미셀농도값($CMC^*$)을 UV 분광광도법으로 측정하였다. 이들 $CMC^*$값들로부터 여러 가지 열역학적 함수값들을 유사상태분리모델(pseudo-phase separation model)을 이용한 식에 의해 계산하였으며 그 값들을 상호 비교하였다. 그 결과 염 및 부탄올 이성질체들을 첨가하였을 때 CPC/TX-100 혼합계면활성제의 미셀화는 순수 물에서의 경우보다 열역학적 함수값($X_1$, $\beta$, ${\gamma}i$, $ai^M$, $C_i$${\Delta}H_{mix}$)들이 큰 차이를 보였으며, 또한 모두 비이상적 혼합미셀모델에 잘 일치하였으며 이상적 혼합미셀모델과는 음방향으로 크게 벗어남을 보였다.

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TP 케이블을 이용하는 이더넷 수신기를 위한 디지털 신호 처리부 설계 (Design of Digital Signal Processor for Ethernet Receiver Using TP Cable)

  • 홍주형;선우명훈
    • 한국통신학회논문지
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    • 제32권8A호
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    • pp.785-793
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    • 2007
  • 본 논문에서는 TP 케이블을 이용하여 100Mbps의 전송 속도를 지원하는 100Base-TX Ethernet 수신기의 디지털 신호 처리부를 제안하였다. 제안하는 디지털 신호 처리부는 자동 이득 조절기, 심볼 동기 복원기, 적응 등화기, BLW 보정기로 구성되어 있으며 초기 위상에 상관없이 150m까지 $10^{-12}BER$이하의 성능을 보였다. 제안하는 신호 처리부는 일부 블록을 제외한 모든 부분을 디지털로 구현하였으며 적응 등화기와 BLW 보정기 연동 구조는 기존의 적응 등화기 에러 값을 이용하는 구조에 비하여 MSE가 약 1dB정도의 성능 향상을 가져왔다. 설계한 디지털 신호 처리부는 Verilog-HDL로 구현되었으며 삼성 $0.18{\mu}m$ 라이브러리를 사용하여 합성 결과 동작 속도는 7.01ns 이며 총 게이트 수는 128.528 게이트였다.

K-PCS와 W-CDMA 듀얼밴드용 유전체 듀플렉서와 밴드패스 필터의 설계 및 제작 (Design and Fabrication of Dielectric Duplexer and Bandpass Filters for K-PCS and W-CDMA Dualband)

  • 최우성;양성현;김철주;문옥식
    • 한국전기전자재료학회논문지
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    • 제25권12호
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    • pp.949-954
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    • 2012
  • The K-PCS and W-CDMA dual band dielectric duplexer and bandpass filters have been designed and fabricated. The dual band duplexer consists of the separate monoblock K-PCS and W-CDMA duplexers using common antenna port. The coupling capacitance and I/O impedance matching have been designed to minimize the cross interference between the bands. Isolations of crosspoint between Tx and Rx in K-PCS and W-CDMA dualband were about 47 dB and 100 dB, respectively. On the other hand, isolations of Tx and Rx in K-PCS and W-CDMA were about 66 dB and 65 dB, respectively. The difference between 47 dB and 100 dB originated from the different center frequencies in Tx and Rx of K-PCS and W-CDMA bands. The coupling capacitance of the bandwidth, I/O capacitance of I/O matching and impedance matching, and various capacitances were important role to fabricate the dielectric duplexer and bandpass filters.

Selection of Suitable Micellar Catalyst for 1,10-Phenanthroline Promoted Chromic Acid Oxidation of Formic Acid in Aqueous Media at Room Temperature

  • Ghosh, Aniruddha;Saha, Rumpa;Ghosh, Sumanta K.;Mukherjee, Kakali;Saha, Bidyut
    • 대한화학회지
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    • 제57권6호
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    • pp.703-711
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    • 2013
  • In the present investigation, kinetic studies of oxidation of formic acid with and without catalyst and promoter in aqueous acid media were studied under the pseudo-first order conditions [formic acid]T ${\gg}[Cr(VI)]_T$ at room temperature. In the 1,10-phenanthroline (phen) promoted path, the cationic Cr(VI) phen complex is the main active oxidant species undergoes a nucleophilic attack by the substrate to form a ternary complex which subsequently experiences a redox decomposition through several steps leading to the products $CO_2$ and $H_2$ along with the Cr(III) phen complex. The anionic surfactant (i.e., sodium dodecyl sulfate, SDS) and neutral surfactant (i.e., Triton X-100, TX-100) act as catalyst and the reaction undergo simultaneously in both aqueous and micellar phase with an enhanced rate of oxidation in the micellar phase. Whereas the cationic surfactant (i.e., N-cetyl pyridinium chloride, CPC) acts as an inhibitor restricts the reaction to aqueous phase. The observed net enhancement of rate effects has been explained by considering the hydrophobic and electrostatic interaction between the surfactants and reactants. The neutral surfactant TX-100 has been observed as the suitable micellar catalyst for the phen promoted chromic acid oxidation of formic acid.

Effects of surfactants on the Fluorescence of Beryllium-Morin Complex

  • Kim, Kang-Jin;Kim, Bok-Hee;Lee, Beom-Gyu;Park, Joon-Woo
    • Bulletin of the Korean Chemical Society
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    • 제9권2호
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    • pp.106-108
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    • 1988
  • The effects of surfactants, CTAB, SDS, and TX-100, on the fluorescence emission intensity of Be-morin complex was studied in terms of pH, temperature, added electrolyte, and concentration of surfactants. The ionic surfactants decreased the fluorescence intensity of the complex. However, the addition of TX-100 caused a substantial increase in the fluorescence intensity without spectral shift, by which means the detection limit of Be was obtained to be 0.3 ppb. Optimum pH for Be determination was near 9.6 and the fluorescence intensity showed a continuous decline with the increase of temperature. Nitrate ion lowered the fluorescence intensity considerably. Mechanism of the interference remains to be elucidated.

Characterization of dental phenotypes and treatment modalities in Korean patients with Parry-Romberg syndrome

  • Yim, Sunjin;Yang, Il-Hyung;Baek, Seung-Hak
    • 대한치과교정학회지
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    • 제50권6호
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    • pp.407-417
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    • 2020
  • Objective: To investigate the dental phenotypes and treatment modalities (Tx-Mod) in Korean patients with Parry-Romberg syndrome (PRS) using longitudinal data. Methods: The samples consisted of 10 PRS patients, who were treated and/or followed-up at Seoul National University Dental Hospital between 1998 and 2019. Using a novel PRS severity index based on the numbers of the atrophy-involved area and asymmetry-involved item, we classified them into mild (n = 3), moderate (n = 2), and severe (n = 5). Dental phenotypes, including congenitally missing tooth (Con-Missing-Tooth), microdontia, tooth with short root (Short-Root), tooth with dilacerated root, and delayed eruption/impacted tooth, were investigated along with Tx-Mod. Results: The side of occurrence of all dental phenotypes showed 100% concordance with the side of PRS involvement. The most two common dental phenotypes were Con-Missing-Tooth and Short-Root (n = 29 and n = 17 in six patients). The sums of the average number of Con-Missing-Tooth and Short-Root increased from mild PRS to moderate PRS and severe PRS cases (1.0, 6.0, and 6.2). In terms of Tx-Mod, growth observation due to mild atrophy, fixed orthodontic treatment, and grafting were used for mild PRS cases. Tx-Mod for moderate PRS cases involved growth observation for surgery due to an early age at the initial visit. For severe PRS cases, diverse Tx-Mod combinations including unilateral functional appliance, fixed orthodontic treatment, growth observation, grafting, and orthognathic surgery were used. Conclusions: The novel PRS severity index may be useful to provide primary data for individualized diagnosis and treatment planning for PRS patients.