• Journal of Animal Reproduction and Biotechnology
• /
• v.37 no.4
• /
• pp.246-254
• /
• 2022

Current studies have revealed the capacity of mesenchymal stem cells (MSCs) in term of immunomodulatory properties, and this distinct potential is downgraded according to the disease duration of patients-derived MSCs. In order to enhance the immunomodulatory and anti-tumorigenic properties of the rheumatoid arthritis (RA) joints-derived MSCs, we aggregate synovial fluid-derived MSCs from RA joints (RA-hMSCs) into 3D-spheroids by the use of hanging drop culture method. Cells were isolated from synovial fluids of RA joints with longstanding active status over 13 years. For aggregation of RA-hMSCs into 3D-spheroids, cells were plated in hanging drops in 30 μL of advanced DMEM (ADMEM) containing 25,000-30,000 cells/drop and cultured for 48 h. To analyze the comparative immunomodulatory effects of 3D-spheroid and 2D monolayer cultured RA-hMSCs and then cells were cultured in ADMEM supplemented with 20% of synovial fluids of RA patients for 48 h and were evaluated by qRT-PCR for their expression of mRNA levels of inflammatory and anti-inflammatory markers. Cellular aggregation of RA-hMSCs was observed and cells were aggregate into a single sphere. Following treatment of RA patient's synovial fluids into the RA-hMSCs, spheroids formed RA-hMSCs showed significantly (p < 0.05) higher expression of TNFα stimulated gene/protein 6 (TSG-6) than the monolayer cultured RA-hMSCs. Therefore, the 3D-spheroid culture methods of RA-hMSCs were more effective than 2D monolayer cultures in suppressing inflammatory response treated with 20% of RA-synovial fluids by expression of TNFα (TSG-6) according to the immune response and enhanced secretion of inflammatory factors.

• Information and Communications Magazine
• /
• v.24 no.3
• /
• pp.87-96
• /
• 2007

HSPA는 HSDPA 및 HSUPA를 통합하여 일컫는 3GPP 기술로 하향 링크에서는 HSDPA를 상향 링크에서는 HSUPA를 사용하여 기존 DCH만을 사용하는 3GPP Release 99 시스템 대비 더 효율적인 고속 멀티미디어 서비스를 제공하는 기술이다. HSDPA는 3GPP Release 5 기술로서 2002년 3월 첫 표준이 승인되었으며 단말의 무선 환경에 따라 변조 및 코딩기법을 변화시키는 AMC, 물리 계층을 통해 빠른 재전송을 지원하는 HARQ,단축된 2ms TTI 그리고 Node-B 기반의 고속 스케줄링을 통해 무선망 성능을 획기적으로 향상시켜 하향 링크에서 최대 14.4Mbps를 제공한다. HSUPA는 3GPP Release 6 기술로서 2004년 6월 첫 표준이 승인되었으며 HSDPA에 도입한 기술들 중에서 AMC를 제외한 모든 기술을 적용하여 상향 링크에서 최대 5.76Mbps를 제공한다. HSPA Evolution(eHSPA 또는 HSPA+)은 HSPA의 성능 개선을 통해 3GPP Release 8 기술인 LTE로의 자연스러운 진화를 보장하기 위한 3GPP Release 7 기술로 2006년 3월 TSG RAN #31 회의에서 승인되었다. 본 고에서는 최근 표준화가 활발히 진행되고 있는 HSPA Evolution에서 최근까지 승인된 각 계층별 요소 기술에 대해 소개하고자 한다.

• Communications of Mathematical Education
• /
• v.10
• /
• pp.393-416
• /
• 2000

수학교육세계회의(數學敎育世界會議)(ICME)는 ICME수학교육국제위원회(數學敎育國際委員會)가 4년마다 한 번씩 개최하는 행사이다. 수학교육세계회의(數學敎育世界會議)(ICME-9)는 올해(2000년) 7월 31일(월)${\sim}$8월 6일(일)에 일본의 Tokyo동경(東京) 근교 Chiba의 Makuhari막장(幕張)서 개최된다. 전세계에서 4,000여명이 참가하는 큰 규모의 수학교육 관련 행사로써 아시아에서는 처음으로 열린다. 특히 이번 ICME에서는 우리 나라 수학교육자들이 많이 참여하게 되었다. 권오남, 박한식, 신현용 교수는 정규 강연(Regular Lecture)을 하게 되었고, 강완 교수 등 일곱 사람은 분과 모임(WGA, TSG)의 조직 위원(Organizer) 등을 맡아 이 행사의 준비 단계에서부터 중요한 역할을 하고 있다. 그 동안 수학교육에 나타난 떠들썩한 이론들은 거의 대부분 서양에서 시작하였고 우리는 이것을 받아들이기에 급급하였다. 그러나 우리의 주위를 살펴보면 우리에게도 전세계에 알릴 것들이 많이 있다. 이러한 것을 찾아서 더욱 갈고 다듬어서 소개할 때가 되었다. 불확실하거나 흩어진 자료들은 체계적으로 정리하는 한편 그 동안 축적된 경험과 정보들은 세밀히 분석하고 또 이론을 세워서 내어 놓아야한다. 우리 모두 ICME-9에 적극적으로 참여하여 새 천년의 우리 나라 수학교육을 한 단계 높여 보자.

• Food Science and Preservation
• /
• v.31 no.3
• /
• pp.452-461
• /
• 2024

This study evaluated the in vitro antioxidant activities of ethanolic Polygonum multiflorum (P. multiflorum) extracts and their cytoprotective effects on H₂O₂-induced HT22 and SK-N-MC cells. Among ethanolic extracts of P. multiflorum, the 40% ethanolic extract of P. multiflorum exhibited high total phenolics and flavonoid contents, with 105.68 mg of GAE/g and 28.92 mg of RE/g, respectively. The 40% ethanolic extract of P. multiflorum showed a high 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity and malondialdehyde (MDA) inhibitory effect. The 40% ethanolic extract of P. multiflorum also showed efficient inhibitory activity against α-glucosidase and acetylcholinesterase. Moreover, the 40% ethanolic extract of P. multiflorum reduced oxidative stress and increased cell viability in H₂O₂-induced HT22 and SK-N-MC cells as determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetra-zoliumbromide (MTT) and 2',7'-dichlorodihydrofluorescein diacetate (DCF-DA) assay. High-performance liquid chromatography (HPLC) identified 2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside (TSG) as the bioactive compound in the 40% ethanolic extract of P. multiflorum.

• Tissue Engineering and Regenerative Medicine
• /
• v.15 no.6
• /
• pp.771-779
• /
• 2018

BACKGROUND: Mesenchymal stromal cells (MSCs) are multipotent stem cells that can differentiate into several cell types. In addition, many studies have shown that MSCs modulate the immune response. However, little information is currently available regarding the maintenance of immunomodulatory characteristics of MSCs through passages. Therefore, we investigated and compared cytokine and gene expression levels from adipose (AD) and bone marrow (BM)-derived MSCs relevant to immune modulation from early to late passages. METHODS: MSC immunophenotype, growth characteristics, cytokine expressions, and gene expressions were analyzed. RESULTS: AD-MSCs and BM-MSCs had similar cell morphologies and surface marker expressions from passage 4 to passage 10. Cytokines secreted by AD-MSCs and BM-MSCs were similar from early to late passages. AD-MSCs and BM-MSCs showed similar immunomodulatory properties in terms of cytokine secretion levels. However, the gene expressions of tumor necrosis factor-stimulated gene (TSG)-6 and human leukocyte antigen (HLA)-G were decreased and gene expressions of galectin-1 and -3 were increased in both AD- and BM-MSCs with repeated passages. CONCLUSION: Our study showed that the immunophenotype and expression of immunomodulation-related cytokines of AD-MSCs and BM-MSCs immunomodulation through the passages were not significantly different, even though the gene expressions of both MSCs were different.

• Tuberculosis and Respiratory Diseases
• /
• v.50 no.6
• /
• pp.668-675
• /
• 2001

Background : Non-smalll lung cancer(NSCLC) develops as a result of the accumulation of multiple genetic abnormalities. Loss of heterozygosity(LOH) is one of the most frequent genetic alterations that is found in NSCLC, and the chromosomal regions that display a high rate of LOH are thought to harbor tumor suppressor genes(TSGs). This study was done to determine the frequency of LOH in 21q with the aim of identifying potential TSG loci. Method : Thirty-nine surgically resected NSCLCs were analysed. Patients peripheral lymphocytes were used as the source of the normal DNA. Five microsatellite Inarkers of 21q were used to study LOH : 21q21.1(D21S1432, and D21S1994); 21q21.2-21.3(D21S1442) ; 21q22.1(21S1445) ; and 21q22.2-22.3(D21S266). The fractional allelic loss(FAL) in a tumor was calculated as the ratio of the number of markers showing LOH to the number of informative markers. Result : LOH for at least one locus was detected in 21 of 39 tumors(53.8%). Among the 21 tumors with LOH, 5(21.8%) showed LOH at almost all informative loci. Although statistically not significant, LOH was found more frequently in squamous cell carcinomas(15 of 23, 65.2%) than in adenocarcinomas(6 of 16, 37.5%). In the squamous cell carcinomas the frequency of LOH was higher in stage II-III (80.0%) than in stage I (53.8%). The FAL value in squamous cell carcinomas($0.431{\pm}0.375$) was significantly higher than that found in adenocarcinomas($0.l92{\pm}0.276$). Conclusion : These results suggest that LOH on 21q may be involved in the development of NSCLC, and that TSG(s) that contribute to the pathogenesis of NSCLC may exist on 21q.

• Molecules and Cells
• /
• v.44 no.3
• /
• pp.146-159
• /
• 2021

DNA methylation, and consequent down-regulation, of tumour suppressor genes occurs in response to epigenetic stimuli during cancer development. Similarly, human oncoviruses, including human papillomavirus (HPV), up-regulate and augment DNA methyltransferase (DNMT) and histone deacetylase (HDAC) activities, thereby decreasing tumour suppressor genes (TSGs) expression. Ubiquitin-like containing PHD and RING finger domain 1 (UHRF1), an epigenetic regulator of DNA methylation, is overexpressed in HPV-induced cervical cancers. Here, we investigated the role of UHRF1 in cervical cancer by knocking down its expression in HeLa cells using lentiviral-encoded short hairpin (sh)RNA and performing cDNA microarrays. We detected significantly elevated expression of thioredoxin-interacting protein (TXNIP), a known TSG, in UHRF1-knockdown cells, and this gene is hypermethylated in cervical cancer tissue and cell lines, as indicated by whole-genome methylation analysis. Up-regulation of UHRF1 and decreased TXNIP were further detected in cervical cancer by western blot and immunohistochemistry and confirmed by Oncomine database analysis. Using chromatin immunoprecipitation, we identified the inverted CCAAT domain-containing UHRF1-binding site in the TXNIP promoter and demonstrated UHRF1 knockdown decreases UHRF1 promoter binding and enhances TXNIP expression through demethylation of this region. TXNIP promoter CpG methylation was further confirmed in cervical cancer tissue by pyrosequencing and methylation-specific polymerase chain reaction. Critically, down-regulation of UHRF1 by siRNA or UHRF1 antagonist (thymoquinone) induces cell cycle arrest and apoptosis, and ubiquitin-specific protease 7 (USP7), which stabilises and promotes UHRF1 function, is increased by HPV viral protein E6/E7 overexpression. These results indicate HPV might induce carcinogenesis through UHRF1-mediated TXNIP promoter methylation, thus suggesting a possible link between CpG methylation and cervical cancer.