Tissue Engineering and Regenerative Medicine

Korean Tissue Engineering and Regenerative Medicine Society (한국조직공학·재생의학회)
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The Expression of Immunomodulation-Related Cytokines and Genes of Adipose- and Bone Marrow-Derived Human Mesenchymal Stromal Cells from Early to Late Passages

  • Mun, Chin Hee (Division of Rheumatology, Department of Internal Medicine, and Department of Medical Sciences, Institute for Immunology and Immunological Disease, Yonsei University College of Medicine) ;
  • Kang, Mi-Il (Division of Rheumatology, Department of Internal Medicine, Dankook University College of Medicine) ;
  • Shin, Yong Dae (Division of Rheumatology, Department of Internal Medicine, and Department of Medical Sciences, Institute for Immunology and Immunological Disease, Yonsei University College of Medicine) ;
  • Kim, Yeseul (Division of Rheumatology, Department of Internal Medicine, and Department of Medical Sciences, Institute for Immunology and Immunological Disease, Yonsei University College of Medicine) ;
  • Park, Yong-Beom (Division of Rheumatology, Department of Internal Medicine, and Department of Medical Sciences, Institute for Immunology and Immunological Disease, Yonsei University College of Medicine)
  • Received : 2018.03.08
  • Accepted : 2018.07.20
  • Published : 2018.12.01
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Abstract

BACKGROUND: Mesenchymal stromal cells (MSCs) are multipotent stem cells that can differentiate into several cell types. In addition, many studies have shown that MSCs modulate the immune response. However, little information is currently available regarding the maintenance of immunomodulatory characteristics of MSCs through passages. Therefore, we investigated and compared cytokine and gene expression levels from adipose (AD) and bone marrow (BM)-derived MSCs relevant to immune modulation from early to late passages. METHODS: MSC immunophenotype, growth characteristics, cytokine expressions, and gene expressions were analyzed. RESULTS: AD-MSCs and BM-MSCs had similar cell morphologies and surface marker expressions from passage 4 to passage 10. Cytokines secreted by AD-MSCs and BM-MSCs were similar from early to late passages. AD-MSCs and BM-MSCs showed similar immunomodulatory properties in terms of cytokine secretion levels. However, the gene expressions of tumor necrosis factor-stimulated gene (TSG)-6 and human leukocyte antigen (HLA)-G were decreased and gene expressions of galectin-1 and -3 were increased in both AD- and BM-MSCs with repeated passages. CONCLUSION: Our study showed that the immunophenotype and expression of immunomodulation-related cytokines of AD-MSCs and BM-MSCs immunomodulation through the passages were not significantly different, even though the gene expressions of both MSCs were different.

Keywords

Acknowledgement

Supported by : National Research Foundation of Korea (NRF)

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