• Korean Journal of Fisheries and Aquatic Sciences
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• v.37 no.4
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• pp.345-353
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• 2004

Target strength (TS) of Japanese common squids (Todarodes pacificus) were measured using 38 and 120 kHz split beam scientific echosounders under the live condition. For the TS measurement of an individual, a total of 3 squids (mantle length (ML): 22.8, 25, and 27 cm) were used using small fishhook method, whereas for measurement of swimming angle, a total of 8 squids (ML: 21-27 cm) were used under live condition, confined with net cage with 2 m diameter At the same time, two underwater video cameras enabled continuous monitoring of squid behavior. Considering normal behavior, the mean TS at 38 and 120 kHz varied from -48.6 to -45.9 dB, and from -46.5 to -44.6 dB, respectively In both frequencies, mean TS at 120 kHz is relatively higher than that of 38 kHz, approximately 1.3-2.5 dB. From free living condition, the mean swimming angle of the squlds was $-24^{\circ}$. The results of the measurement will be provided basic information for conducting acoustic surveys of the squid.

• Molecules and Cells
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• v.19 no.2
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• pp.219-222
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• 2005

The a-subunit of Escherichia coli tryptophan synthase (${\alpha}TS$), a component of the tryptophan synthase ${\alpha}_2{\beta}_2$ complex, is a monomeric 268-residues protein (Mr = 28,600). ${\alpha}TS$ by itself catalyzes the cleavage of indole-3-glycerol phosphate to glyceraldehyde-3-phosphate and indole, which is converted to tryptophan in tryptophan biosynthesis. Wild-type and P28L/Y173F double mutant ${\alpha}$-subunits were overexpressed in E. coli and crystallized at 298 K by the hanging-drop vapor-diffusion method. X-ray diffraction data were collected to $2.5{\AA}$ resolution from the wild-type crystals and to $1.8{\AA}$ from the crystals of the double mutant, since the latter produced better quality diffraction data. The wild-type crystals belonged to the monoclinic space group C2 ($a=155.64{\AA}$, $b=44.54{\AA}$, $c=71.53{\AA}$ and ${\beta}=96.39^{\circ}$) and the P28L/Y173F crystals to the monoclinic space group $P2_1$ ($a=71.09{\AA}$, b=52.70, $c=71.52{\AA}$ and ${\beta}=91.49^{\circ}$). The asymmetric unit of both structures contained two molecules of ${\alpha}TS$. Crystal volume per protein mass ($V_m$) and solvent content were $2.15{\AA}^3\;Da^{-1}$ and 42.95% for the wild-type and $2.34{\AA}^3\;Da^{-1}$ and 47.52% for the double mutant.

• Journal of the Korean Society for Marine Environment & Energy
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• v.15 no.2
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• pp.133-141
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• 2012

Effects of temperature and salinity on the growth of the toxic dinoflagellate $Alexandrium$ $tamarense$ and $A.$ $catenella$ isolated from the southern coast of Korea were examined in the laboratory. Growth experiment was conducted under the following combinations of temperature and salinity; 10, 15, 20, 25 and $30^{\circ}C$, 10, 15, 20, 25, 30 and 35 psu at a constant irradiance of 300 ${\mu}mol$ photons $m^{-2}s^{-1}$. Temperature and salinity conditions for maximum growth rate were indicated as follows: temperature $15^{\circ}C$ and salinity 30 psu (0.31 $d^{-1}$) in $A.$ $tamarense$, temperature $25^{\circ}C$ and salinity 30 psu (0.36 $d^{-1}$) in A. catenella. Temperature and salinity ranges for optimum growth condition of two species were $10{\sim}20^{\circ}C$, 25~35 psu and $120{\sim}30^{\circ}C$, 25~35 psu, respectively. The result of two-factor ANOVA indicated significant effects (P<0.001) of temperature and salinity on the growth rate, and two species were more effected by a temperature than a salinity on the growth. In addition, prediction equations were obtained through the multiple regressions of the specific growth rates as ${\mu}=0.04+0.0193T-0.0339S- 0.0005T^2+0.0021S^2+0.00073TS-0.000022T^3-0.000038S^3+0.00000086TS^2-0.0000255T^2S$ in $A.$ $tamarense$ and ${\mu}=1.01-0.1288T-0.0778S+0.0067T^2+0.0038S^2+0.00204TS-0.0001T^3-0.000059S^3-0.0000131TS^2-0.0000392T^2S$ in $A.$ $catenella$. Correlation coefficient between experimental values and simulated values was highly indicated. These results seem to provide information for understanding the spreading mechanism of $A.$ $tamarense$ and $A.$ $catenella$.

• Progress in Superconductivity and Cryogenics
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• v.8 no.3
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• pp.37-40
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• 2006

Oxide buffer layers for YBCO coated conductors were fabricated using MOD processing and development of microstructure and texture were investigated. A $CeO_2$ buffer layers were formed on RABiTS tape. Acetate-based precursor solution was employed to synthesize the precursor solution. Subsequently, the precursor solution was stabilized and modified with triethanolamine. $CeO_2$ precursor gel film was coated and annealed in $Ar/H_2$ atmosphere at high temperature. An annealed $CeO_2$ film shows mixed orientation with high (001) texturing. It was shown that (111) texture of $CeO_2$ layers were enhanced by multiple coating. This degradation was attributed to development of microcracks in the multiply coated $CeO_2$ films. Also discussed are the synthesis and the characterization of $La_2Zr_2O_7$ (LZO) buffer layers on RABiTS tape. A biaxially textured LZO buffer layer was fabricated with MOD processing method using metal alkoxide based precursor solution. It was shown that the LZO film were epitaxially grown on RABiTS tape and crack-free & uniform surface was obtained after annealing in $Ar/H_2$ atmosphere.

• IMMUNE NETWORK
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• v.22 no.4
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• pp.35.1-35.16
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• 2022

Tobacco smoking (TS) has been known as one of the most potent risk factors for airway inflammatory diseases. However, there has been a paucity of information regarding the immunologic alteration mediated by TS in patients with chronic rhinosinusitis with nasal polyps (CRSwNP). To identify the effect of TS, we harvested human tissue samples (never smoker: n=41, current smoker: n=22, quitter: n=23) and analyzed the expression of epithelial-derived cytokines (EDCs) such as IL-25, IL-33, and thymic stromal lymphopoietin. The expressions of Th2 cytokines and total serum IgE showed a type-2 inflammatory alteration by TS. In addition, the epithelial marker E-cadherin and epithelial-mesenchymal transition (EMT)-associated markers (N-cadherin, α-SMA, and vimentin) were evaluated. Histological analysis showed that EDC expressions were upregulated in the current smoker group and downregulated in the quitter group. These expression patterns were consistent with mRNA and protein expression levels. We also found that the local Th2 cytokine expression and IgE class switching, as well as serum IgE levels, were elevated in the current smoker group and showed normal levels in the quitter group. Furthermore, the expressions of E-cadherin decreased while those of N-cadherin, α-SMA, and vimentin increased in the current smoker group compared those in the never smoker group. Taken together, these results indicate that TS contributes to the deterioration of pathogenesis by releasing local EDCs and Th2 cytokines, resulting in EMT in patients with CRSwNP. We verified that alterations of immunological response by TS in sinonasal epithelium can play a vital role in leading to CRSwNP.

• Proceedings of the Korea Information Processing Society Conference
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• 2009.04a
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• pp.462-465
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• 2009

모바일 응용 S/W GUI를 기존의 매뉴얼을 보고 테스트 하는 단계적이고 수동적인 방법을 탈피하여 사용자 중심의 시나리오를 통하여 자동으로 GUI를 테스트 하는 것을 목적으로 한다. 제시한 테스트 방법은 사용자 중심의 UI를 설계하여 간편하고, 정확하게 테스트 할 뿐만 아니라 테스트케이스를 재사용하는 환경을 지원한다. 제안한 자동화 도구는 시나리오 기반의 정확한 테스트와 재사용성을 높인 테스트 도구로서 기존 테스트 도구와 기능적인 비교를 통하여 성능을 확인하였다.

• Korean Journal of Environmental Agriculture
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• v.29 no.2
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• pp.197-205
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• 2010

This study was conducted to investigate the optimum conditions for efficient methane production through anaerobic digestion of pig waste slurry. The examined parameters were organic matter content of the pig slurry, the ratio of seed sludge to pig slurry, and stirring intensity of the digestion reactor. The effects of types of slurry produced from different purpose-based pigs fed with different feeds were also tested. The methane concentration in the produced biogas was 45% when the ratio of seed sludge to pig slurry was 50% and total solid (TS) concentration was 1%, and it increased in proportional to TS concentration increases from 3 to 7%. At 3 and 5% of TS concentration, increasing mixing velocity from 80 to 160 rpm resulted in higher biogas production amount. However, mixing amount of seed sludge did not cause any significant effect on biogas production. Overall, the most efficient biogas production was achieved at 3-5% TS concentration in combination with 50% seed sludge inoculation and mixing velocity at 120 rpm. Among pig slurry types, gestating sow waste slurry showed the highest biogas production probably due to higher the degradation rate than other types of pig waste slurry being affected by the feeds components.

• Journal of Science Education
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• v.36 no.2
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• pp.216-234
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• 2012

This study examined the characteristics of verbal interactions presented in TS activities with different tasks' openness levels by the cognitive levels of students through the implementation of TS program to 14 fifth graders in gifted class. Results of this study revealed that the open-type TS activities showed higher percentages of verbal interactions than the guiding-type TS activities showed and that the higher the open level of tasks was, the more high-level verbal interactions occurred. These results were showed in almost all subcomponents of verbal interactions. The results according to the students' cognitive levels showed that the higher the cognitive level of students was, higher frequency of interactions, high-level verbal interactions and a variety of verbal interactions occurred. The influence of both cognitive level of students and the task's openness on verbal interactions among students seemed to be interactive, however. In guiding-type activities, the percentage of high-level verbal interactions was not high although the cognitive level of students was high. And students in low level of cognition showed far lower frequency of interactions and their percentage of high-level verbal interactions was low even though the openness of the tasks was high. The results of this study meant that although open-type activities drew higher level verbal interactions by stimulating students' thought, the effects would be limited owing to their low cognitive level. Based on these findings, an implication was suggested that it is important to design instructional strategies and adjust openness level of TS activities to students' cognitive level so as to stimulate the thinking of students in lower cognitive level and to highten their engagement in activities.

• The Journal of Internal Korean Medicine
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• v.26 no.3
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• pp.551-562
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• 2005

Objectives : The aim of this study was to investigate the effect of Trichosanthis Semen Herbal-acupuncture(TS-HA) at Joksamni(ST36) on ovalbumin-induced asthma in mice. Methods : C57BL/6 mice were sensitized and challenged with ovalbumin(OVA) once a week for twelve weeks. The experimental group was treated with 1% concentrations of TS-HA at Joksamni(ST36) three times a week for the last eight weeks. Results : 1. The weight and total lung cells of the mice in the group treated with TS-HA decreased significantly compared with those of control group. 2. Total leukocytes and eosinophils in Bronchoalveoler-lavage fluid(BALF) of the mice in the group treated with TS-HA decreased significantly compared with those of control group. 3. Eosinophils in BALF of the mice in the group treated with TS-HA in photomicrographs decreased significantly compared with those of control group. 4. According to histological analysis of lung sections, adhension of collagen in TS-HA decreased significantly compared with that of control group. 5. The concentration of IgE, IL-4, IL-5 in BALF and IL-4, IL-5, IL-13 in serum of the mice in the group treated with TS-HA decreased significantly compared with that of control group. 6. The number of $Gr-^+/CD11b^+,\;CD11b^+,\;CD3e^-/CCR3^+,\;CD4^+,\;CD8^+\;CD3e^+/CD69^+,\;CD23^+/B220^+\;cells$ in the lungs of the mice in the group treated with TS-HA decreased significantly compared with those of control group. Conclusion : These results suggest that Trichosanthis Semen-herbal acupuncture at Joksamni(ST36) done on C57BL/6 mice is effective in part in relieving OVA-induced asthma in C57BL/6 mice.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.37 no.2
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• pp.133-139
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• 2001

In order to obtain fundamental data for estimation of fisheries resource by echo sounder, we carried out the measuring of dorsal aspect Target strengths for rock bream and dusky spinefoot fishes that were caught much around the Jeju Island and in South Sea of Korea. The appropriate equations share the common form. TS=A+20 log L, where TS is the average dorsal aspect target strength in decibels, L is the fish total length in centimeters, and the coefficient A is determined by a least mean squares regression analysis. For rock bream, the result is TS=-72.97+20 log L and, for dusky spinefoot it is TS=-63.16+20 log L And, we have investigated the bearing range of maximum dorsal aspect target strength for all of rock bream and dusky spinefoot by the echo sounder with transducer of which frequency is 200kHz. They are $-12^\circ$-$-21^\circ$and $-1^\circ$--8 espectively, when the fishes is swimming down to the bottom. The maximum dorsal target strengths are -41.50dB at -18 or rock bream and -30.69dB at $-6^\circ$for dusky spinefoot.