• Molecules and Cells
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• 제38권9호
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• pp.759-764
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• 2015

Tripartite motif protein 25 (TRIM25), mediates K63-linked polyubiquitination of Retinoic acid inducible gene I (RIG-I) that is crucial for downstream antiviral interferon signaling. Here, we demonstrate that TRIM25 is required for melanoma differentiation-associated gene 5 (MDA5) and MAVS mediated activation of NF-${\kappa}B$ and interferon production. TRIM25 is required for the full activation of NF-${\kappa}B$ at the downstream of MAVS, while it is not involved in IRF3 nuclear translocation. Mechanical studies showed that TRIM25 is involved in TRAF6-mediated NF-${\kappa}B$ activation. These collectively indicate that TRIM25 plays an additional role in RIG-I/MDA5 signaling other than RIG-I ubiquitination via activation of NF-${\kappa}B$.

• Molecules and Cells
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• 제42권10호
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• pp.721-728
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• 2019

Non-structural protein 1 (NS1) of influenza virus has been shown to inhibit the innate immune response by blocking the induction of interferon (IFN). In this study, we isolated two single-stranded RNA aptamers specific to NS1 with $K_d$ values of $1.62{\pm}0.30nM$ and $1.97{\pm}0.27nM$, respectively, using a systematic evolution of ligand by exponential enrichment (SELEX) procedure. The selected aptamers were able to inhibit the interaction of NS1 with tripartite motif-containing protein 25 (TRIM25), and suppression of NS1 enabled retinoic acid inducible gene I (RIG-I) to be ubiquitinated regularly by TRIM25. Additional luciferase reporter assay and quantitative real-time PCR (RT-PCR) experiments demonstrated that suppression of NS1 by the selected aptamers induced IFN production. It is noted that viral replication was also inhibited through IFN induction in the presence of the selected aptamers. These results suggest that the isolated aptamers are strongly expected to be new therapeutic agents against influenza infection.

• 에너지공학
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• 제13권2호
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• pp.118-127
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• 2004

본 연구는 배기량이 16.7ι인 V8형 터보차져 인터쿨러 직접분사식 디젤기관에서 흡기포트의 선회유동과 연료분사계 및 과급기가 기관성능 및 배출가스특성에 미치는 영향을 실험적으로 고찰하며 성능을 개선하는데 있다. 일반적으로 기관의 출력을 높이기 위하여 과급기 및 인터쿨러를 장착하여 과급공기를 냉각시켜 과급효율을 더욱 높인 TCI디젤기관이 보편화되고 있다 본 연구의 결과로서 흡기포트의 선회비가 2.25인 경우에서 압축비 17.5, re-entrant 8.5$^{\circ}$ 형 연소실, 노즐분공경 $\Phi$0.33*3+$\Phi$0.35*2, 노즐돌출량 3.18mm, 분사시기 BTDC 12$^{\circ}$CA, 과급기 T042(압축기 0.6A/R+46Trim, 터빈 1.0A/R+57Trim)경우가 기관성능 및 NO$_{x}$ 농도의 배출특성을 고려할 때 운전영역에서 가장 우수하여 흡기포트, 분사계 및 과급기에 대한 각 인자를 적정화할 수 있었다.

• International Journal of Oral Biology
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• 제35권3호
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• pp.113-119
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• 2010

Porphyromonas (P.) gingivalis lipopolysaccharide (Pg LPS) is the major pathogenic component of periodontal disease. In this study, we have attempted to determine the expression profiles of the signal transduction pathway genes induced by Pg LPS in comparison with Escherichia (E.) coli LPS (Ec LPS). DC2.4 cells were treated for two hours with $1\;{\mu}g/ml$ of Pg LPS or $0.5\;{\mu}g/ml$ of Ec LPS. The total RNA from these cells was then isolated and reverse-transcribed. Gene expression profiles were then analyzed with a signal transduction pathway finder GEArray Q series kit and significant changes in expression were confirmed by real-time PCR. The microarray results indicated that several genes, including Tnfrsf10b, Vcam1, Scyb9, Trim25, Klk6, and Stra6 were upregulated in the DC2.4 cells in response to Pg LPS treatment, but were downregulated or unaffected by Ec LPS. Realtime PCR revealed that the expression of Trim25, Scyb9 and Tnfrsf10b was increased over the untreated control. Notably, Trim25 and Tnfrsf10b were more strongly induced by Pg LPS than by Ec LPS. These results provide greater insight into the signal transduction pathways that are altered by P. gingivalis LPS.

• 대한기계학회논문집A
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• 제25권3호
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• pp.537-547
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• 2001

The paper proposes the fuzzy logic global approximate optimization strategies in optimal sizing of automotive A-pillar trim with rib structures for occupant head protection. Two different strategies referred to as evolutionary fuzzy modeling (EFM) and neuro-fuzzy modeling (NFM) are implemented in the context of global approximate optimization. EFM and NFM are based on soft computing paradigms utilizing fuzzy systems, neural networks and evolutionary computing techniques. Such approximation methods may have their promising characteristics in a case where the inherent nonlinearity in analysis model should be accommodated over the entire design space and the training data is not sufficiently provided. The objective of structural design is to determine the dimensions of rib in A-pillar, minimizing the equivalent head injury criterion HIC(d). The paper describes the head-form modeling and head impact simulation using LS-DYNA3D, and the approximation procedures including fuzzy rule generation, membership function selection and inference process for EFM and NFM, and subsequently presents their generalization capabilities in terms of number of fuzzy rules and training data.

• 해양환경안전학회지
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• 제25권5호
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• pp.601-608
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• 2019

본 연구에서는 선수 돌출의 반전형 선수 형상을 가진 고속 쌍동선의 선체부착 부가물에 의한 주행성능 영향에 대하여 수치해석과 회류수조 모형시험을 통하여 비교분석하였다. 반전형 선수 형상은 재래식 선수 형상보다 선수 발산파 파정의 생성위치를 선미방향으로 이동시켜 개선된 조파형상을 보이며, 저항 및 안정된 항주자세에 효과적임을 보였다(Kim et al., 2019). 본 연구에서의 반전형 선수 내측에 부착된 핀과 선미단 인터셉터(Interceptor)에 의한 파형과 항주자세 변화 등 주행성능에 대한 모형시험 결과에서는, 1) 반전형 선수의 Trim 특성 2) Fin에 의한 내측 파의 중첩 개선 3) Fin과 Interceptor에 의한 자세제어는 두 선체 연결갑판(Wetdeck) 충격을 줄이는데 효과적인 것으로 판단된다.

• 수산해양기술연구
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• 제44권1호
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• pp.68-74
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• 2008

This study is concerned with the development of the basic planing hull form of small fishing boat in 25 knots high speed. A series of model test to determine the optimum performance hull form of actual fishing boat with 10 gross tonnage was carried out for 5 models made available planing hull form in the circulation water channel. Model test was performed with the resistance test to study the hydrodynamic characteristics of model ships and the sinkage and trim measurement to investigate the stability of model ships and also the wave pattern observation to analyze the effectiveness of model ships. As the result, the planing hull form of P-4 with deep V type bow can be derived as the best hull form with good performance especially in ship's resistance efficiency showing less residual resistance and sinkage and trim and the spray effect, etc..

• 한국산업융합학회 논문집
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• 제25권4_2호
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• pp.541-547
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• 2022

Before making a prototype, we predicted the inlet/outlet differential pressure and flow coefficient, which are the most basic design data for the valve through the design and numerical analysis of the trim, which is the most important in the localization development of the 1500Ib high differential pressure control valve used for boiler feed water. As a result, the design value and the analysis value were found to be about 98% similar. The flow field within the fluid velocity of 23m/s to prevent cavitation was also found. The result of the numerical analysis on thermal stress due to the characteristics of valves exposed to high temperatures showed that it was found to be about 18% less than the allowable stress of the bolt fixing the trim. When all loads such as pressure, self-weight, and vibration are applied, however, it is judged to go beyond the currently calculated thermal stress, exceeding the allowable stress.

• 한국자동차공학회논문집
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• 제13권4호
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• pp.135-144
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• 2005

The Effects of intake swirl and combustion parameters on the performance and emission characteristics in a V8 type turbocharged intercooler D.I. diesel engine of the displacement $16.7\iota$ were studied experimentally in this paper. Generally the swirl in the combustion process of diesel engine promotes mixing of the injection fuel and the intake air. Also, TCI diesel engine is put to practically use intercooler in order to increase boost efficiency which is cooled boost air. As a result of steady flow test, when the swirl ratio is increased, the mean flow coefficient is decreased, whereas the Gulf factor is increased. And through engine test, its can be effected to meet performance and emission by optimizing the main parameters; the swirl ratio is 2.25, compression ratio is 17.5, combustion bowl is re-entrant $8.5^{\circ}$, nozzle hole diameter is $\phi0.33^{\ast}3+\phi0.35^{\ast}2$, injection timing is BTDC $12^{\circ}CA$ and turbocharger is T02 model which are compressor 0.6A/R+46trim and turbine 1.0A/R+57trim.

• Journal of Advanced Marine Engineering and Technology
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• 제29권4호
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• pp.443-452
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• 2005

The purpose of this study is experimentally to analyze the effects of intake port swirl, injection system and turbocharger on the engine performance and the emission characteristics in a V8 type turbocharger intercooler D.I. diesel engine of the displacement 16.7L, and to suggest the improvement of engine performance. Generally to enhance engine power, TCI diesel engine is put to practically use turbocharged intercooler in order to increase volume efficiency which is cooled boost air. As results of considering the factors of the intake port of swirl ratio 2.25, compression ratio 17.5. re-entrant $8.5^{\circ}$ combustion bowl, nozzle hole diameter ${\phi}0.33{\ast}3+{\phi}0.35{\ast}2$. nozzle protrusion 3.18mm, injection timing BTDC $12^{\circ}CA$ and turbocharger(compressor 0.6A/R+46Trim. turbine 1.0 A/R+57Trim) is the best in the full load in the engine performance and the exhaust characteristics of NOx concentration. Therefore. their factors are appropriated as intake system, injection and turbocharger system.