Browse > Article

Induction of Signal Transduction Pathway Genes in Dendritic Cells by Lipopolysaccharides from Porphyromonas gingivalis and Escherichia coli  

Jin, Ho-Kyeong (Department of Oral Microbiology, School of Dentistry, Pusan National University)
Lee, Young-Hwa (Department of Oral Microbiology, School of Dentistry, Pusan National University)
Jeong, So-Yeon (Department of Oral Microbiology, School of Dentistry, Pusan National University)
Na, Hee-Sam (Department of Oral Microbiology, School of Dentistry, Pusan National University)
Park, Hae-Ryoun (Department of Oral Pathology, School of Dentistry, Pusan National University)
Chung, Jin (Department of Oral Microbiology, School of Dentistry, Pusan National University)
Publication Information
International Journal of Oral Biology / v.35, no.3, 2010 , pp. 113-119 More about this Journal
Abstract
Porphyromonas (P.) gingivalis lipopolysaccharide (Pg LPS) is the major pathogenic component of periodontal disease. In this study, we have attempted to determine the expression profiles of the signal transduction pathway genes induced by Pg LPS in comparison with Escherichia (E.) coli LPS (Ec LPS). DC2.4 cells were treated for two hours with $1\;{\mu}g/ml$ of Pg LPS or $0.5\;{\mu}g/ml$ of Ec LPS. The total RNA from these cells was then isolated and reverse-transcribed. Gene expression profiles were then analyzed with a signal transduction pathway finder GEArray Q series kit and significant changes in expression were confirmed by real-time PCR. The microarray results indicated that several genes, including Tnfrsf10b, Vcam1, Scyb9, Trim25, Klk6, and Stra6 were upregulated in the DC2.4 cells in response to Pg LPS treatment, but were downregulated or unaffected by Ec LPS. Realtime PCR revealed that the expression of Trim25, Scyb9 and Tnfrsf10b was increased over the untreated control. Notably, Trim25 and Tnfrsf10b were more strongly induced by Pg LPS than by Ec LPS. These results provide greater insight into the signal transduction pathways that are altered by P. gingivalis LPS.
Keywords
signal transduction; Porphyromonas gingivalis; Escherichia coli; lipopolysaccharide; dendritic cell;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Banchereau J. Steinman RM. Dendritic cells and the control of immunity. Nature. 1998;392:245-52.   DOI   ScienceOn
2 Cutler CW, Jotwani R, Palucka KA, Davoust J, Bell D, Banchereau J. Evidence and a novel hypothesis for the role of dendritic cells and Porphyromonas gingivalis in adult periodontitis. J Periodont Res. 1999;34:406-12.   DOI   ScienceOn
3 Kinane DF, Lappin DF, Koulouria O, Buckly A. Humoralimmune responses in periodontal disease may have mucosal and systemic immune features. Clin Exp Immunol. 1999;115: 534-41.   DOI   ScienceOn
4 Lorenz E, Patel DD, Hartung T, Schwartz DA. Toll-like receptor 4 (TLR4)-deficient murine macrophage cell line as an in vitro assay system to show TLR4-independent signaling of Bacteroides fragilis lipopolysaccharide. Infect Immun. 2002;70:4892-6.   DOI   ScienceOn
5 Strieter RM, Polverini PJ, Kunkel SL, Arenberg DA, Burdick MD, Kasper J, Dzuiba J, Damme JV, Walz A, Marriott D, Chan SY, Roczniak S. The Functional Role of the ELR Motif in CXC Chemokine-mediated Angiogenesis. Biochem Mol Biol. 1995;270:27348-57.
6 Tanamoto K, Azumi S, Haishima Y, Kumada H, Umemoto T. The lipid A moiety of Porphyromonas gingivalis lipopolysaccharide specifically mediates the activation of C3H/HeJ mice. J Immunol. 1997;158:4430-6.
7 Vanguri P, Farber JM. Identification of CRG-2. An interferoninducible mRNA predicted to encode a murine monokine J. Biol. Chem. 1990;265:15049-57.
8 Van Winkelhoff AJ, Van Steenbergen TJ, De Graaff J. The role of black-pigmented Bacteroides in human oral infections. J Clin Periodontol. 1988;15:145-55.   DOI
9 Westphal O, Jann K. Bacterial lipopolysaccharides: extraction with phenol water and further applications of the procedure. Methods Carbohydr Chem. 1965;5:83.
10 Liao F, Rabin RL, Yannelli JR, Koniaris LG, Vanguri P. Human Mig chemokine: biochemical and functional characterization J Exp Med. 1995;182:1301-14.   DOI   ScienceOn
11 Lipshutz RJ, Fodor SP, Gingeras TR, Lockhart DJ. High density synthetic oligonucleotide arrays. Nat Genet. 1999; 21:20-4.   DOI   ScienceOn
12 Loetscher M, Gerber B, Loetscher P, Jones SA, Piali L, Clark- Lewis I, Baggiolini M, Moser B. Chemokine receptor specific for IP10 and mig: structure, function, and expression in activated T-lymphocytes. J Exp Med. 1996;184:963-9.   DOI   ScienceOn
13 Luster AD, Unkeless JC, Ravetch JV. Gamma-interferon transcriptionally regulates an early-response gene containing homology to platelet proteins. Nature. 1985;315:672-6.   DOI   ScienceOn
14 Narumi S, Tominaga Y, Tamaru M, Shimai S, Okumura H, Nishioji K, Itoh Y, Okanoue T. Expression of IFN-inducible protein-10 in chronic hepatitis. J Immunol. 1997;158:5536-44.
15 MacFarlane M, Ahmad M, Srinivasula SM. Identification and molecular cloning of two novel receptors for the cytotoxic ligand TRAIL. J Biol Chem 1997;272:25417-20.   DOI   ScienceOn
16 Nagai Y, Akashi S, Nagafuku M, Ogata M, Iwakura Y, Akira S, Kitamura T, Kosugi A, Kimoto M, Miyake K. Essential role of MD-2 in LPS responsiveness and TLR4 distribution. Nat Immunol. 2002;3:667-72.   DOI
17 Nair BC, Mayberry WR, Dziak R, Chen PB, Levine MJ, Hausmann E. Biological effects of a purified lipopolysaccharide from Bacteroides gingivalis. J Periodontal Res. 1983;18:40-9.   DOI
18 Ogawa T. Chemical structure of lipid A from Porphyromonas (Bacteroides) gingivalis lipopolysaccharide. FEBS Lett. 1993; 332:197-201.   DOI   ScienceOn
19 Ogawa T. Immunobiological properties of chemically defined lipid A from lipopolysaccharide of Porphyromonas (Bacteroides) gingivalis. Eur J Biochem. 1994;219:737-42.   DOI   ScienceOn
20 Sachiko IB, Ciric B, Christophi GP, Bernett MJ, Blaber M, Rodriguez M, Scarisbrick IA. Targeting kallikrein 6 proteolysis attenuates CNS inflammatory disease. FASEB J. 2004;18: 920-2.   DOI
21 Amichay D, Gazzinelli R, Karupiah G, Moench T, Sher A, Farber J. Genes for chemokines MuMig and Crg-2 are induced in protozoan and viral infections in response to IFN-gamma with patterns of tissue expression that suggest nonredundant roles in vivo. J Immunol. 1996;157:4511-20.
22 Asensio VC, Campbell IL. Chemokine gene expression in the brains of mice with lymphocytic choriomeningitis. J Virol. 1997;10:7832-40.
23 Barksby HE, Nile CJ, Jaedicke KM, Taylor JJ, Preshaw PM. Differential expression of immunoregulatory genes in monocytes in response to Porphyromonas gingivalis and Escherichia coli lipopolysaccharide. Clin Exp Immunol. 2009;156:479-87.   DOI   ScienceOn
24 Inoue S, Orimo A, Hosoi T, Kondo S, Toyoshima H, Kondo T, Ikegami A, Ouchi Y, Orimo H, Muramatsu M. Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein. Proc Natl Acad Sci U S A. 1993;90:11117-21.   DOI   ScienceOn
25 Fearon DT, Locksley RM. The instructive role of innate immunity in the acquired immune responses. Science. 1996; 272:50-4.   DOI   ScienceOn
26 Hart DN. Dendritic cells: unique leukocyte populations which control the primary immune responses. Blood. 1997;90: 3245-87.
27 Hirschfeld M, Weis JJ, Toshchakov V, Salkowski CA, Cody MJ, Ward DC, Qureshi N, Michalek SM, Vogel SN. Signaling by toll-like receptor 2 and 4 agonists results in differential gene expression in murine macrophages. Infect Immun. 2001;69:1477-82.   DOI   ScienceOn
28 Kan L, Okiji T, Kaneko T, Suda H. Localization and density of myeloid leukocytes in the periodontal ligament of normal rat molars. Arch Oral Biol. 2001;46:509-20.   DOI   ScienceOn
29 Kirikae T, Nitta T, Kirikae F, Suda Y, Kusumoto S, Qureshi N, Nakano M. Lipopolysaccharides (LPS) of oral blackpigmented bacteria induce tumor necrosis factor production by LPS-refractory C3H/HeJ macrophages in a way different from that of Salmonella LPS. Infect Immun. 1999;67:1736-42.
30 Sakurai K, Okiji T, Suda H. Co-increase of nerve fibre and HLS-DR- and/or factor-XIIIa-expressing dendritic cells in dental caries-affected regions of the human dental pulp: An immunohistochemical study. J Dent Res. 1999;78:1596-608.   DOI   ScienceOn
31 Sallusto F, Cella M, Danieli C, Lanzavecchia A. Dendritic cells use macropinocytosis and the mannose receptor to concentrate macromolecules in the major histocompatibility complex class II compartment: down-regulation by cytokines and bacterial products. J Exp Med. 1995;182:389-400.   DOI   ScienceOn
32 Saurin AJ, Borden KL, Boddy MN, Freemont PS. Does this have a familiar RING? Trends Biochem Sci. 1996;21:208-14.   DOI
33 Strieter RM, Polverini PJ, Arenberg DA, Kunkel SL. The role of CXC chemokines as regulators of angiogenesis. Shock. 1995;4:155-60.   DOI   ScienceOn