• Title/Summary/Keyword: TRIM25

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Regulation of MDA5-MAVS Antiviral Signaling Axis by TRIM25 through TRAF6-Mediated NF-κB Activation

  • Lee, Na-Rae;Kim, Hye-In;Choi, Myung-Soo;Yi, Chae-Min;Inn, Kyung-Soo
    • Molecules and Cells
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    • v.38 no.9
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    • pp.759-764
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    • 2015
  • Tripartite motif protein 25 (TRIM25), mediates K63-linked polyubiquitination of Retinoic acid inducible gene I (RIG-I) that is crucial for downstream antiviral interferon signaling. Here, we demonstrate that TRIM25 is required for melanoma differentiation-associated gene 5 (MDA5) and MAVS mediated activation of NF-${\kappa}B$ and interferon production. TRIM25 is required for the full activation of NF-${\kappa}B$ at the downstream of MAVS, while it is not involved in IRF3 nuclear translocation. Mechanical studies showed that TRIM25 is involved in TRAF6-mediated NF-${\kappa}B$ activation. These collectively indicate that TRIM25 plays an additional role in RIG-I/MDA5 signaling other than RIG-I ubiquitination via activation of NF-${\kappa}B$.

Recovery of TRIM25-Mediated RIG-I Ubiquitination through Suppression of NS1 by RNA Aptamers

  • Woo, Hye-Min;Lee, Jin-Moo;Kim, Chul-Joong;Lee, Jong-Soo;Jeong, Yong-Joo
    • Molecules and Cells
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    • v.42 no.10
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    • pp.721-728
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    • 2019
  • Non-structural protein 1 (NS1) of influenza virus has been shown to inhibit the innate immune response by blocking the induction of interferon (IFN). In this study, we isolated two single-stranded RNA aptamers specific to NS1 with $K_d$ values of $1.62{\pm}0.30nM$ and $1.97{\pm}0.27nM$, respectively, using a systematic evolution of ligand by exponential enrichment (SELEX) procedure. The selected aptamers were able to inhibit the interaction of NS1 with tripartite motif-containing protein 25 (TRIM25), and suppression of NS1 enabled retinoic acid inducible gene I (RIG-I) to be ubiquitinated regularly by TRIM25. Additional luciferase reporter assay and quantitative real-time PCR (RT-PCR) experiments demonstrated that suppression of NS1 by the selected aptamers induced IFN production. It is noted that viral replication was also inhibited through IFN induction in the presence of the selected aptamers. These results suggest that the isolated aptamers are strongly expected to be new therapeutic agents against influenza infection.

A Study on the Performance Improvement in a V8 Type Turbocharged Intercooler D.I. Diesel Engine (V8형 터보차져 인터쿨러 직접분사식 디젤기관의 성능개설에 관한 연구)

  • 석동현;윤준규;차경옥
    • Journal of Energy Engineering
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    • v.13 no.2
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    • pp.118-127
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    • 2004
  • The purpose of this study is experimentally to analyze that intake port swirl, injection system and turbocharger have an effect on the engine performance and the emission characteristics in a V8 type turbocharged intercooler D.I. diesel engine of the displacement 16.7ι, and to suggest the improvement of engine performance. Generally to enhance engine power, TCI diesel engine is put to practically use turbo-charged intercoler in order to increase boost efficiency which is cooled boost air. As results of considering the factors of the intake port of swirl ratio 2.25, compression ratio 17.5, re-entrant 8.5$^{\circ}$ combustion bowl, nozzle hole diameter ${\Phi}$0.33*3+${\Phi}$0.35*2, nozzle protrusion 3.18mm, injection timing BTDC 12$^{\circ}$CA and turbo charger (compressor 0.6A/R+46Trim, turbine 1.0A/R+57Trim) is the best in the full range of operating in the engine performance and the exhaust characteristics of NO$\_$x/ concentration. Therefore their factors are appropriated as intake system, injection and turbocharger system.

Induction of Signal Transduction Pathway Genes in Dendritic Cells by Lipopolysaccharides from Porphyromonas gingivalis and Escherichia coli

  • Jin, Ho-Kyeong;Lee, Young-Hwa;Jeong, So-Yeon;Na, Hee-Sam;Park, Hae-Ryoun;Chung, Jin
    • International Journal of Oral Biology
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    • v.35 no.3
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    • pp.113-119
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    • 2010
  • Porphyromonas (P.) gingivalis lipopolysaccharide (Pg LPS) is the major pathogenic component of periodontal disease. In this study, we have attempted to determine the expression profiles of the signal transduction pathway genes induced by Pg LPS in comparison with Escherichia (E.) coli LPS (Ec LPS). DC2.4 cells were treated for two hours with $1\;{\mu}g/ml$ of Pg LPS or $0.5\;{\mu}g/ml$ of Ec LPS. The total RNA from these cells was then isolated and reverse-transcribed. Gene expression profiles were then analyzed with a signal transduction pathway finder GEArray Q series kit and significant changes in expression were confirmed by real-time PCR. The microarray results indicated that several genes, including Tnfrsf10b, Vcam1, Scyb9, Trim25, Klk6, and Stra6 were upregulated in the DC2.4 cells in response to Pg LPS treatment, but were downregulated or unaffected by Ec LPS. Realtime PCR revealed that the expression of Trim25, Scyb9 and Tnfrsf10b was increased over the untreated control. Notably, Trim25 and Tnfrsf10b were more strongly induced by Pg LPS than by Ec LPS. These results provide greater insight into the signal transduction pathways that are altered by P. gingivalis LPS.

Application of Soft Computing Based Response Surface Techniques in Sizing of A-Pillar Trim with Rib Structures (승용차 A-Pillar Trim의 치수설계를 위한 소프트컴퓨팅기반 반응표면기법의 응용)

  • Kim, Seung-Jin;Kim, Hyeong-Gon;Lee, Jong-Su;Gang, Sin-Il
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.25 no.3
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    • pp.537-547
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    • 2001
  • The paper proposes the fuzzy logic global approximate optimization strategies in optimal sizing of automotive A-pillar trim with rib structures for occupant head protection. Two different strategies referred to as evolutionary fuzzy modeling (EFM) and neuro-fuzzy modeling (NFM) are implemented in the context of global approximate optimization. EFM and NFM are based on soft computing paradigms utilizing fuzzy systems, neural networks and evolutionary computing techniques. Such approximation methods may have their promising characteristics in a case where the inherent nonlinearity in analysis model should be accommodated over the entire design space and the training data is not sufficiently provided. The objective of structural design is to determine the dimensions of rib in A-pillar, minimizing the equivalent head injury criterion HIC(d). The paper describes the head-form modeling and head impact simulation using LS-DYNA3D, and the approximation procedures including fuzzy rule generation, membership function selection and inference process for EFM and NFM, and subsequently presents their generalization capabilities in terms of number of fuzzy rules and training data.

A Study on the Effect of Hull Appendages of High-Speed Catamarans with Modified-reverse bow on the Running Performance (반전형 선수부 형상을 갖는 고속 쌍동선의 부가물이 주행성능에 미치는 영향에 관한 연구)

  • Kim, Do-jung;Na, Hyun-ho;Kim, Jung-eun;Oh, Do-won;Choi, Hong-sik
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.25 no.5
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    • pp.601-608
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    • 2019
  • In this study, the effect of hull appendages on the high-speed catamarans with reverse bow shapes is compared and analyzed by numerical analysis and circulating water tank model test. The reverse bow shape showed an improved wave shape by shifting the generation position of forward divergent wave to the stern direction and was effective in resistance and stable running posture (Kim et al., 2019). In the model test results of the running performance as the wave patterns and the change of the running posture due to the fin fitted with the inner side of the inverted bow and the interceptor, 1) Trim characteristics of the inverted bow 2) Improvement of superposition of inner wave by Fin 3) The trim control by the fin and the interceptor is considered to be effective in reducing the impact of the two hull connection decks (wetdeck).

Hull form development of the high speed small fishing boat (고속 소형 어선의 기본선행 개발)

  • Lee, Kwi-Joo;Joa, Soon-Won
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.44 no.1
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    • pp.68-74
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    • 2008
  • This study is concerned with the development of the basic planing hull form of small fishing boat in 25 knots high speed. A series of model test to determine the optimum performance hull form of actual fishing boat with 10 gross tonnage was carried out for 5 models made available planing hull form in the circulation water channel. Model test was performed with the resistance test to study the hydrodynamic characteristics of model ships and the sinkage and trim measurement to investigate the stability of model ships and also the wave pattern observation to analyze the effectiveness of model ships. As the result, the planing hull form of P-4 with deep V type bow can be derived as the best hull form with good performance especially in ship's resistance efficiency showing less residual resistance and sinkage and trim and the spray effect, etc..

A Study on the Flow Coefficient Test and Numerical Analysis about 1500lb High-Pressure Drop Control Valve for Boiler Feedwater Pump (보일러 급수펌프용 1500lb 고차압 제어밸브 유량시험 및 수치해석에 관한 연구)

  • Lee, Kwon-Il;Jang, Hoon;Lee, Chi-Woo
    • Journal of the Korean Society of Industry Convergence
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    • v.25 no.4_2
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    • pp.541-547
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    • 2022
  • Before making a prototype, we predicted the inlet/outlet differential pressure and flow coefficient, which are the most basic design data for the valve through the design and numerical analysis of the trim, which is the most important in the localization development of the 1500Ib high differential pressure control valve used for boiler feed water. As a result, the design value and the analysis value were found to be about 98% similar. The flow field within the fluid velocity of 23m/s to prevent cavitation was also found. The result of the numerical analysis on thermal stress due to the characteristics of valves exposed to high temperatures showed that it was found to be about 18% less than the allowable stress of the bolt fixing the trim. When all loads such as pressure, self-weight, and vibration are applied, however, it is judged to go beyond the currently calculated thermal stress, exceeding the allowable stress.

Effects of Intake Swirl and Combustion Parameters on the Performance and Emission in a V8 Type Turbocharged Intercooler Diesel Engine (흡기 선회유동 및 연소인자가 V8형 TCI 디젤엔진의 성능 및 배출가스특성에 미치는 영향)

  • Yoon Junkyu;Cha Kyungok
    • Transactions of the Korean Society of Automotive Engineers
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    • v.13 no.4
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    • pp.135-144
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    • 2005
  • The Effects of intake swirl and combustion parameters on the performance and emission characteristics in a V8 type turbocharged intercooler D.I. diesel engine of the displacement $16.7\iota$ were studied experimentally in this paper. Generally the swirl in the combustion process of diesel engine promotes mixing of the injection fuel and the intake air. Also, TCI diesel engine is put to practically use intercooler in order to increase boost efficiency which is cooled boost air. As a result of steady flow test, when the swirl ratio is increased, the mean flow coefficient is decreased, whereas the Gulf factor is increased. And through engine test, its can be effected to meet performance and emission by optimizing the main parameters; the swirl ratio is 2.25, compression ratio is 17.5, combustion bowl is re-entrant $8.5^{\circ}$, nozzle hole diameter is $\phi0.33^{\ast}3+\phi0.35^{\ast}2$, injection timing is BTDC $12^{\circ}CA$ and turbocharger is T02 model which are compressor 0.6A/R+46trim and turbine 1.0A/R+57trim.

A Study on the Emission Reduction and Performance Improvement in a V8 Type TCI D.I. Diesel Engine (V8형 TCI 디젤기관의 배출가스저감 및 성능개선에 관한 연구)

  • Yoon Jun-Kyu;Lim Jong-Han
    • Journal of Advanced Marine Engineering and Technology
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    • v.29 no.4
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    • pp.443-452
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    • 2005
  • The purpose of this study is experimentally to analyze the effects of intake port swirl, injection system and turbocharger on the engine performance and the emission characteristics in a V8 type turbocharger intercooler D.I. diesel engine of the displacement 16.7L, and to suggest the improvement of engine performance. Generally to enhance engine power, TCI diesel engine is put to practically use turbocharged intercooler in order to increase volume efficiency which is cooled boost air. As results of considering the factors of the intake port of swirl ratio 2.25, compression ratio 17.5. re-entrant $8.5^{\circ}$ combustion bowl, nozzle hole diameter ${\phi}0.33{\ast}3+{\phi}0.35{\ast}2$. nozzle protrusion 3.18mm, injection timing BTDC $12^{\circ}CA$ and turbocharger(compressor 0.6A/R+46Trim. turbine 1.0 A/R+57Trim) is the best in the full load in the engine performance and the exhaust characteristics of NOx concentration. Therefore. their factors are appropriated as intake system, injection and turbocharger system.