• The Korean Journal of Physiology and Pharmacology
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• v.24 no.6
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• pp.555-561
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• 2020

TWIK-related two-pore domain K⁺ channel-2 (TREK-2) has voltage-independent activity and shows additional activation by acidic intracellular pH (pH_i) via neutralizing the E³³² in the cytoplasmic C terminal (Ct). We reported opposite regulations of TREK-2 by phosphatidylinositol 4,5-bisphosphate (PIP₂) via the alkaline K³³⁰ and triple Arg residues (R^355-357); inhibition and activation, respectively. The G³³⁴ between them appeared critical because its mutation (G³³⁴A) endowed hTREK-2 with tonic activity, similar to the mutation of the inhibitory K³³⁰ (K³³⁰A). To further elucidate the role of putative bent conformation at G³³⁴, we compared the dual mutation forms, K³³⁰A/G³³⁴A and G³³⁴A/R^355-7A, showing higher and lower basal activity, respectively. The results suggested that the tonic activity of G³³⁴A owes to a dominant influence from R^355-7. Since there are additional triple Arg residues (R^377-9) distal to R^355-7, we also examined the triple mutant (G³³⁴A/R^355-7A/R^377-9A) that showed tonic inhibition same with G³³⁴A/R^355-7A. Despite the state of tonic inhibition, the activation by acidic pH_i was preserved in both G³³⁴A/R^355-7A and G³³⁴A/R^355-7A/R^377-9A, similar to the R^355-7A. Also, the inhibitory effect of ATP could be commonly demonstrated under the activation by acidic pH_i in R^355-7A, G³³⁴A/R^355-7A, and G³³⁴A/R^355-7A/R^377-9A. These results suggest that the putative bent conformation at G³³⁴ is important to set the tug-of-war between K³³⁰ and R^355-7 in the PIP₂-dependent regulation of TREK-2.

• The Korean Journal of Physiology and Pharmacology
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• v.9 no.1
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• pp.63-68
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• 2005

Hydrogen peroxide ($H_2O_2$) causes oxidative stress and is considered as an inducer of cell death in various tissues. Two-pore domain $K^+$ ($K_{2p}$) channels may mediate $K^+$ efflux during apoptotic volume decreases (AVD) in zygotes and in mouse embryos. In the present study, we sought to elucidate linkage between $K_{2p}$ channels and cell death by $H_2O_2$. Thus $K_{2p}$ channels (TASK-1, TASK-3, TREK-1, TREK-2) were stably transfected in HEK-293 cells, and cytotoxicity assay was preformed using cell counting kit-8 (CCK-8). Cell survival rates were calculated using the cytotoxicity assay data and dose-response curve was fitted to the $H_2O_2$ concentration. Ionic currents were recorded in cell-attached mode. The bath solution was the normal Ringer solution and the pipette solution was high $K^+$ solution. In HEK-293 cells expressing TREK-1, TREK-2, TASK-3, $H_2O_2$ induced cell death did not change in comparison to non-transfected HEK-293. In HEK-293 cells expressing TASK-1, however, dose-response curve was significantly shifted to the left. It means that $H_2O_2$ induced cell death was increased. In cell attached-mode recording, application of $H_2O_2$ (300μM) increased activity of all $K_{2p}$ channels. However, a low concentration of $H_2O_2$ ($50{\mu}M$) increased only TASK-1 channel activity. These results indicate that TASK-1 might participate in $K^+$ efflux by $H_2O_2$ at low concentration, thereby inducing AVD.

• Proceedings of the IEEK Conference
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• 2008.06a
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• pp.335-336
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• 2008

By using DM(Diagnostic Monitoring) data measured at WiBro network, automatic optimization function of WiBro network is implemented in this paper. The optimization function mentioned is able to be run on PC with 2GHz CPU and 1 GB memory. Automatic optimization function is one module of CellTREK that is a wireless network planning and optimization software developed by Infra Lab., KT.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.35 no.1
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• pp.83-89
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• 2017

The Jeju Olle Trail Route No. 1 has opened in 2007, and over one million visitors per year trek total of 26 Olle trails throughout the island of Jeju as of 2017. The Olle trekking is one of eco-friendly tourism activities in the era of social issues with environmental collapse and threats. Up until now, studies on the Jeju Olle trail are focused on the visitors while the studies on residents who are the other major pillar of Jeju Olle trail have been limited. The study aims to compare the visit types, satisfaction level and pro-environmental behaviors between the visitors and residents on Jeju Olle trails. Total of 290 survey was collected in three month period from December, 2016 to February, 2017. The survey result indicates the Jeju residents trek the Jeju Olle in a larger group than the Jeju Olle visiting trekkers and show lower level in the visit satisfaction and pro-environmental behaviors. The noticeable lower level in the pro-environmental behaviors of residents can directly connect to the issue of Jeju environment as a whole, and the continuing environmental education program and promotion for residents to improve the pro-environmental behavior level is essential.

• Korean Journal of Applied Biomechanics
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• v.26 no.3
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• pp.315-321
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• 2016

Objective: The aim of this study was to analyze effects of the toe-spring angle of bobsleigh shoes on start speed lap time to develop Korean-specific bobsled shoes suitable for winter environments and for domestic players on the basis of sports science and optimized biomechanical performance. Method: Seven Korean bobsleigh athletes participated in this study, with three pairs of sprint spikes from three companies (Type A, Type B, Type C). To analyze sprint lap time and forefoot bending angle for each shoe, participants were instructed to drag a sled 15 meters from the start line at a maximum sprint. forefoot bending angle was collected by a high speed camera, and lap time speed was measured. Results: Lap time for type B shoes was $3.52{\pm}0.17sec$, type A was $3.55{\pm}0.19sec$, and type C was $3.56{\pm}0.18sec$. Forefoot bending angles were: angle 1, $6.88{\pm}5.55^{\circ}$; angle 2, $9.23{\pm}6.38^{\circ}$; angle 3, $15.56{\pm}5.39^{\circ}$; angle 4, $9.54{\pm}3.85^{\circ}$; angle 5, $9.22{\pm}5.08^{\circ}$; angle 6, $7.66{\pm}6.44^{\circ}$; and angle 7, $4.30{\pm}6.24^{\circ}$ (p<.001). Forefoot bending in angle 3 was as follows: type A, $16.47{\pm}6.01^{\circ}$; type B, $14.30{\pm}4.96^{\circ}$; and type C, $15.90{\pm}5.17^{\circ}$. Conclusion: Hard outsoles and midsoles are better than soft type for reduced start lap time when developing a prototype Korean bobsled shoe.

• Archives of Plastic Surgery
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• v.49 no.2
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• pp.240-252
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• 2022

Rather than just another "review," this is intended to be an "overview" of the entire subject of the medial sural artery perforator (MSAP) flap as has been presented in the reconstructive literature from its inception in 2001 until the present, with any exceptions not purposefully overlooked. Unfortunately, the pertinent anatomy of the MSAP flap is always anomalous like most other perforator flaps, and perhaps even more variable. No schematic exists to facilitate the identification of a dominant musculocutaneous perforator about which to design the flap, so some adjunctive technology may be highly valuable for this task. However, if a relatively thin free flap is desirable for a small or moderate sized defect that requires a long pedicle with larger caliber vessels, the MSAP flap deserves consideration. Indeed, for many, this has replaced the radial forearm flap such as for partial tongue reconstruction. Most consider the donor site deformity, even if only a conspicuous scar on the calf, to be a contraindication. Yet certainly if used as a local flap for the knee, popliteal fossa, or proximal leg, or as a free flap for the ipsilateral lower extremity where a significant recipient site deformity already exists, can anyone really object that this is not a legitimate indication? As with any perforator flap, advantages and disadvantages exist, which must be carefully perused before a decision to use the MSAP flap is made. Perhaps not a "workhorse" flap for general use throughout the body, the MSAP flap in general may often be a valuable alternative.

• Journal of the Institute of Convergence Signal Processing
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• v.12 no.3
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• pp.222-227
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• 2011

2-axis tracking solar PV system applying a fixed-type unit than in the same area as the panel's power output to more than 140% can be obtained that have been identified. However, this approach compared with fixed or 1-axis control system and control the complexity of trekking equipment power the cursor comes to a relatively small output PV unit is called. In this paper, a small PV power units as a way to improve the economics of the small output of multiple PV units in the central control unit in enclosed places an intermittent manner by a remote control for each unit of the trek at the same time to simplify the control mechanism to reduce power that will be introduced. also the construction of large-scale PV development plans in difficult environments can be utilized in a manner appropriate to introduce.