• Journal of Microbiology and Biotechnology
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• v.19 no.3
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• pp.217-228
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• 2009

Mobile genetic segments, or transposons, are also referred to as jumping genes as they can shift from one position in the genome to another, thus inducing a chromosomal mutation. According to the target site-specificity of the transposon during a transposition event, the result is either the insertion of a gene of interest at a specific chromosomal site, or the creation of knockout mutants. The former situation includes the integration of conjugative transposons via site-specific recombination, several transposons preferring a target site of a conserved AT-rich sequence, and Tn7 being site-specifically inserted at attTn7, the downstream of the essential glmS gene. The latter situation is exploited for random mutagenesis in many prokaryotes, including IS (insertion sequence) elements, mariner, Mu, Tn3 derivatives (Tn4430 and Tn917), Tn5, modified Tn7, Tn10, Tn552, and Ty1, enabling a variety of genetic manipulations. Randomly inserted transposons have been previously employed for a variety of applications such as genetic footprinting, gene transcriptional and translational fusion, signature-tagged mutagenesis (STM), DNA or cDNA sequencing, transposon site hybridization (TraSH), and scanning linker mutagenesis (SLM). Therefore, transposon-mediated genetic engineering is a valuable discipline for the study of bacterial physiology and pathogenesis in living hosts.

• Electric Engineers Magazine
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• v.228 no.8
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• pp.52-57
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• 2001

Presently the Korean grounding system uses TN system, multi-grounding method with IT independent grounding method. Nevertheless TN system can't exist with TT system in the technological terms. If they coexist, it causes ground-fault circuit not to operate, and brings about different electrical potential rise by customer system. It brings about serious problems for safety. This paper aims for improving method of grounding system based on the technical analysis on instances in foreign countries and Korea. Almost standards and construction manner were apt to be internationalized after WTO/TBT agreement was concluded. The internal grounding systems should meet the international criteria and reliability for safety, and be provided with technologically impeccable standards.

• BMB Reports
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• v.31 no.5
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• pp.453-458
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• 1998

Human thrombopoietin (hTPO) was expressed to high levels in insect cells using the baculovirus expression system. Full-length hTPO cDNA containing a native signal peptide sequence was amplified by PCR from a human fetal liver cDNA library and cloned into the Autographa californica nuclear polyhedrosis virus (AcNPV) expression vector. Immunoblot analysis with antiserum against hTPO indicated that an approximately 55 kDa protein was produced in recombinant AcNPV infected insect cells. Recombinant hTPO was produced 4-fold higher in Trichoplusia ni (Tn5) cells than in Spodoptera frugiperda (Sf9) cells. with most of the hTPO produced in Tn5 cells secreted into the culture medium. Addition of tunicamycin in the culture medium resulted in the reduction of the size of hTPO to 35-38 kDa, and most of the protein remained within the cell. These results suggest that N-glycosylation of hTPO is required for the secretion of the protein into the culture medium in insect cells. hTPO produced in insect cells induced proliferation and maturation of megakaryocyte progenitors, indicating that it is in a biologically active form.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.23 no.5
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• pp.36-41
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• 2009

In this paper an Investigation into the actual condition of electrical equipment installation for the inspection method development based on IEC 60364. The analysis objects are the difference between electrical equipments installation in korea & electrical equipments installation based on the IEC 60364. The measurement elements are loop impedance, operating characteristic of circuit breaker and protective conductor continuance. As a result, the korea electrical equipments installation was almost same the TT system but some different of protective conductor continuance. Electrical equipments installed TN-C-S has noting problem about installation but manager dose not understand about IEC 60364.

• Korean Journal of Ecology and Environment
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• v.56 no.4
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• pp.339-347
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• 2023

Dissolved organic matter (DOM) is a key component in the biogeochemical cycling in freshwater ecosystem. However, it has been rarely explored, particularly complex river watershed dominated by natural and anthropogenic sources, such as various effluent facility and livestock. The current research developed a new analytical method for TOC/TN (Total Organic Carbon/Total Nitrogen) stable isotope ratio, and distinguish DOM source using stable isotope value (δ¹³C-DOC) and spectroscopic indices (fluorescence index [FI] and biological index [BIX]). The TOC/TN-IR/MS analytical system was optimized and precision and accuracy were secured using two international standards (IAEA-600 Caffein, IAEA-CH-6 Sucrose). As a result of controlling the instrumental conditions to enable TOC stable isotope analysis even in low-concentration environmental samples (<1 mgC L^-1), the minimum detection limit was improved. The 12 potential DOM source were collected from watershed, which includes top-soils, groundwater, plant group (fallen leaves, riparian plants, suspended algae) and effluent group (pig and cow livestock, agricultural land, urban, industry facility, swine facility and wastewater treatment facilities). As a result of comparing characteristics between 12 sources using spectroscopic indices and δ¹³C-DOC values, it were divided into four groups according to their characteristics as a respective DOM sources. The current study established the TOC/TN stable isotope analyses system for the first time in Korea, and found that spectroscopic indices and δ¹³C-DOC are very useful tool to trace the origin of organic matter in the aquatic environments through library database.

• Korean Journal of Veterinary Service
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• v.40 no.4
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• pp.287-292
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• 2017

A five-year-old, male, Great Dane weighing 107 kg was presented with anorexia, abdominal distension, and dyspnea for 5 days. Physical examination, blood works, radiography, electrocardiography (ECG), and echocardiography were performed. Based on severely low fractional shortening (FS) and marked four chamber enlargement in echocardiography, continuous atrial fibrillation and occasional ventricular premature complex (VPC) on ECG, the dog was diagnosed as dilated cardiomyopathy (DCM) concurrent with congestive heart failure. Pleural effusion and ascites were modified transudate. In accordance with DCM scoring system recommended by European Society of Veterinary Cardiology (ESVC), DCM score was 13/15 in this case. Concentrations of cTnI and NT-pro-BNP were 1.0 ng/mL and 693 pmol/L, respectively. Since the former and the latter were remarkably high values, it was certain that the patient had grave prognosis. Intensive care was performed for the dog and the clinical signs as well as the radiographic abnormalities were resolved. However, when he presented serious dyspnea again at 25 days post therapy, the dog was dead. In case of canine DCM, the scoring system for the diagnosis and cardiac biomarkers including NT-pro-BNP and cTnI could be useful to advise owners on the status and prognosis of their dog with DCM.

• Proceedings of the KSR Conference
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• 2011.10a
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• pp.1459-1467
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• 2011

This paper proposes the effective method to improve the protection from induced lightning Surge by making common grounding from individual grounding. Common grounding under equipotential principle is more effective than individual grounding for lightning Surge protection, and so common grounding is indicated as international technical standard under the AC power supply system with neutral line. So, this paper is to propose the effective way of induced lightning Surge protection method for currently installed power supply system which has no neutral grounded line and individual grounding which are weak for lightning Surge protection. This proposal can improve the power supply system as has neutral line, and improve the grounding system to common grounding system. And also this paper proposes to make effective equipotential system with voltage variable shunting devices for lightning Surge protection.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2013.10a
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• pp.215-218
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• 2013

This paper proposes the effective method to improve the protection from induced lightning surge by making common grounding from individual grounding. Common grounding under equipotential principle is more effective than individual grounding for lightning surge protection, and so common grounding is indicated as international technical standard under the AC power supply system with neutral line. So, this paper is to propose the effective way of induced lightning surge protection method for currently installed power supply system which has no neutral grounded line and individual grounding which are weak for lightning surge protection. This proposal can improve the power supply system as has neutral line, and improve the grounding system to common grounding system. And also this paper proposes to make effective equipotential system with voltage variable shunting devices for lightning surge protection.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 1997.11a
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• pp.497-500
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• 1997

Conducting polymer is new material in lithium secondary battery. conducting polymer has a lot of merit which is flexible and good handing so that this material is used battery system, solid polymer electrolytes airs used PEO(Polyethylene oxide) and PEO/PMMA branding material adding by liquid plasticizer or lithium salt polymer electrolyte which is added liquid plasticizer, lithium salt decreased the crystallity and thermal stability is over than 13$0^{\circ}C$. it is very useful tn apply lithium secondary battery system.

• Korean Journal of Environmental Biology
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• v.35 no.4
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• pp.654-661
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• 2017

Recently, a low-impact development (LID) technic such as a wetland has been proposed as a Nature-friendly process for reducing pollutants caused by livestock wastewater. Therefore, the Daphnia magna toxicity was analyzed for livestock wastewater samples, to determine if a wetland system would also be effective in reducing this ecotoxicity. In the present study, acute D. magna toxicity was not significantly dependent on the presence and type of reed, nor type of media. However, when treated with construction wetlands, ecotoxicities decreased as well as TN, TP and COD concentrations. Therefore, it is considered that a construction wetland system with bio reeds and bio-media as well as general reeds would be effective to reduce the ecotoxicity of livestock wastewater. To apply a wetland system as the subsequent treatment process to a livestock waste water treatment facility, it is necessary to perform an integrated evaluation such as treatment efficiency and the ecotoxicity test for various characteristics of livestock wastewater.