• Title/Summary/Keyword: TN

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Isolation and Characterization of Tn5 Insertion Mutants of Pseudomonas fluorescens Antagonistic to Rhizoctonia solani (Rhizoctonia solani 길항세균 Pseudomonas fluorescens의 Tn5 삽입 돌연변이주 분리 및 특성)

  • 박서기;박기범;김기청
    • Korean Journal Plant Pathology
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    • v.10 no.1
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    • pp.39-46
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    • 1994
  • Pseudomonas fluorescens Biovar III strains S-2 antagonistic to Rhizoctonia solani was subjected to Tn5 mutagenesis by the transposon vector pGS9. Ampicillin and kanamycin resistant (Ampr, Kmr) transconjugants were recovered at a frequency of 1.3$\times$10-7 per initial recipient cell, when recipient cells were washed twice in TE buffer before conjugation. Of the ca. 3000 transconjugants, a frequency of noninhibitory (Inh-), nonfluorescent (Flu-) and auxotorphic (Pro-) mutants were 0.27%, 0.47% and 0.40%, respectively. In these mutants, all Inh- mutants showed the same colony morphology as wild type, whereas all Flu- and Pro- mutants inhibited the growth of R. solani. These mutants were also susceptible to chloramphenicol, indicating only the Tn5 element, except for parts of pGS9, was integrated into the recipient genome. In a Southern blot analysis, the Tn5 element inserted into one site on the chromosome for each of the chosen mutants. However, Tn5 insertion sites of Inh-, and Pro- mutants were differed in each other. These indicate that the genes essential for R. solani inhibition, fluorescent production and auxotrophic are chromosomally located, but not linked to each other.

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Neutron dosimetry with a pair of TLDs for the Elekta Precise medical linac and the evaluation of optimum moderator thickness for the conversion of fast to thermal neutrons

  • Marziyeh Behmadi;Sara Mohammadi;Mohammad Ehsan Ravari;Aghil Mohammadi;Mahdy Ebrahimi Loushab;Mohammad Taghi Bahreyni Toossi;Mitra Ghergherehchi
    • Nuclear Engineering and Technology
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    • v.56 no.2
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    • pp.753-761
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    • 2024
  • Introduction: In this study, TLD 600 and TLD 700 pairs were used to measure the neutron dose of Elekta Precise medical linac. To this end, the optimum moderate thickness for the conversion of fast to thermal neutrons were evaluated. Materials and methods: 241Am-Be and 252Cf sources were simulated to calculate the optimum thicknesses of the moderator for the conversion of maximum fast neutrons (FN) into thermal neutrons (TN). Pair TLDs were used to measure F&TN doses for three different field sizes at four depths of the medical linac. Results: The maximum thickness of the moderator was optimized at 6 cm. The measurement results demonstrated that the TN dose increased with the expansion of field size and depth. The FN dose, which was converted TN, exhibits behaviors comparable to the TN due to its nature. Conclusion: This study presents the optimum thickness for the moderator to convert FN into TN and measure F&TN using TLDs.

Design of a Single-Polarizer Reflective TN LCD (단일 편광판을 이용한 반사형 TN LCD의 설계)

  • 김희정;이기동;윤태훈;김재창
    • Proceedings of the Optical Society of Korea Conference
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    • 2000.08a
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    • pp.232-233
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    • 2000
  • 반사형 액정표시소자는 저전력 휴대용 표시장치 시장이 급격하게 성장함에 따라 큰 주목을 받고 있다. 현재 반사형 액정표시소자는 TN 액정 모드를 기본적으로 사용하고 있다. 본 논문에서는 편광판을 한 장 사용하여 빛의 흡수를 줄여 밝기를 높일수 있고, 광대역 λ/4 film을 사용하여 명암 대비를 높일 수 있는 단일 편광판 모드 반사형 TN LCD를 설계하고 제작하였다. (중략)

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A New Approach to Teaching Modern Chinese Words of Locality 'qian+Tn' (효율적인 중국어교학을 위한 '전(前)+Tn'의 시간의미 분석)

  • Park, Minsoo
    • Cross-Cultural Studies
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    • v.30
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    • pp.245-258
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    • 2013
  • This paper analyzes the current Chinese prepositions that represent space and time, which ultimately indicate the concept of Direction. having this in mind, the concept of time that the prepositional phrases 'qian+Tn' and 'Tn+qian' possess can be understood by analyzing the prepositions that 'qian' to make time expressions. also, this paper establishes a systemic framework that will help not only students but also professors to use the preposition 'qian' in order to make correct time expressions.

A Study of the Level of Cardiac Troponin I in Patients with Clinically Suspected Acute Myocarditis Treated with Intravenous Gammaglobulin (심근형 Troponin I의 증가로 급성 심근염의 진단을 받은 환아에서 정맥용 면역글로부린 치료 효과에 대한 연구)

  • Kim, Minshik;Lee, Youngok;Chun, Yoonae
    • Pediatric Infection and Vaccine
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    • v.5 no.2
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    • pp.267-275
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    • 1998
  • Purpose : We have studied the changes of cardiac troponin I(cTnI) level and left ventricular ejection fraction(LVEF) before and after treatment of IVGG to evaluate the efficacy of single high dose of intravenous gammaglobulin(IVGG)(2.0gm/kg) therapy for improving cardiac function and clinical symptoms and signs in patients with clinically suspected acute myocarditis. Methods : The patients consisted of 18 cases who admitted increased cTnI with clinically suspected acute myocarditis caused by viral infection, Kawasaki disease and fever unknown origin(FUO) from Jan. 1995 to Jun. 1998. The control group consisted of 20 cases suffered from hand-foot-mouth disease, herpangina and high fever with rash. The level of cTnI was measured by Chemiluminiscent immunoassay method(normal<0.1ng/ml) and cardiac function was evaluated by left ventricular ejection fraction(LVEF)(normal 64~83%) by echocardiogram. Results : The level of cTnI increased to $0.306{\pm}0.209ng/ml$ and LVEF decreased to $60.1{\pm}1.6%$ before treatment of IVGG significantly as compared with control group(P<0.05). All cases were returned to normal range of LVEF($71.4{\pm}3.7%$) and decreased cTnI significantly($0.089{\pm}0.082ng/ml$) after treated with IVGG within 1 week in patients group(P<0.05). Conclusion : The single high dose of IVGG(2.0gm/kg) therapy was rapid and effective improvement of cardiac function and clinical symptoms and signs of acute myocarditis, and the measurement of serum cTnI and LVEF may help to diagnose and evaluate efficacy of IVGG on it.

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Change Pattern Analysis of the COD and Nutrient Concentration in Jumunjin Harbour, Gangneung (강릉 주문진항 COD 및 영양염류의 농도변화 양상 분석)

  • Cho Hong-Yean;Kim Chang-Il;Lee Dal-Soo;Han Dong-Jun
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.18 no.3
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    • pp.211-224
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    • 2006
  • It was analysed that spatial and temporal change patterns of COD, TN and TP were measured monthly from 2002 to 2005 in Jumunjin Harbour. Cenerally, concentration of upper layer higher than lower layer, concentration difference of COD, TN and TP on upper lower layer at inner zone in Jumunjin Harbour higher than sea area front of breakwater. Seasonal change width of COD concentration different, but seasonal change pattern of COD, $NH_{4}-N$ and TN clearly showed. Water quality improvement effect of seawater exchange facilities and sewage treatment plants is analysed quantitatively using averaged spatial and temporal data set. Change of COD small at 2003, concentration reduced about $34{\sim}47%$ at all zones on 2004 and 2005. TN was influenced largely effect of seawater exchange facilities. Concentration reduction at zone 1, 2, 3 estimated about $60{\sim}70%,\;40{\sim}60%,\;40%$, respectively. As a consequence, concentration reduction of COD and TN that is, effect of water quality improvement influenced seawater exchange facilities appeared not only at inner zone, also sea area front of breakwater and entrance of Jumunjin Harbour.

The Effect of Breed, Parity, Litter Size, Lactating Period, and Estrus Interval on Sow Productivity Traits in Swine (모돈의 생산능력에 미치는 품종, 산차, 산자수, 포유기간 및 발정재귀일령의 효과)

  • 연정웅
    • Korean Journal of Animal Reproduction
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    • v.25 no.3
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    • pp.251-257
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    • 2001
  • This study was carried out to investigate the effect of breed, parity, litter size, lactating period, and estrus interval on sow productivity traits in swine. Data from 492 heads of Landrace, Yorkshire or crossbred sow which were raised at Yonam College of Agriculture from March, 1998 to July, 2000 were analyzed for this study. The total number of pigs born (TN), the number of pigs born alive (NB), and the number of pigs suckled (NS) were greater in the crossbred sows than in the purebred. In TN and NB, the 3rd to 7th parities were greater than other parities, and the 8th parity was the lowest. The number of pigs stillbirthed (NSB) and the number of runt per litter tended to increase with the increase of TN. In addition, statistical analysis showed that parity had significant effect on most of traits. The current TN had highly significant effect on TN, NB and NS of the next parity As current TN increased, TN, NB, and NS of the next parity increased. The current lactating period also affected significantly for TN, NB, and NS of the next parity The sows which had the lactating period of 20∼21 days produced the greatest TN and NB in the next parity. Weaning to estrus interval(WEI) had significant effect on TN, NB, and NSB. Among WEI groups, the WEI group of 7∼13 days was the lowest in TN, NB and NSB.

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CHANGES IN LIVE-WEIGHT GAIN, BLOOD CONSTITUENTS AND WORM EGG COUNTS IN THAI NATIVE AND CROSS-BRED GOATS RAISED IN VILLAGE ENVIRONMENTS IN SOUTHERN THAILAND

  • Kochapakdee, S.;Pralomkarn, W.;Choldumrongkul, S.;Saithanoo, S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.8 no.3
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    • pp.241-247
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    • 1995
  • The aim of the present study was to investigate the effect of internal parasites on growth rates of Thai Native (TN) and crossbred (75% TN $\times$ 25% Anglo-Nubian, AN and 50% TN $\times$ 50% AN) goats (undrenched, drenched every 3 weeks or at 9 weeks) in village environments in southern Thailand in a humid tropical climate. There was no significant (p > 0.05) difference in growth rate ($g/kg^{0.75}/d$) between the genotypes during unsupplemented grazing (0-64 days of the experimental period). However, during supplementary feeding (64-127 days) and throughout the period (0-127 days) TN goats had significantly (p < 0.01) lower growth rates compared with 75% TN $\times$ 25% AN and 50% TN $\times$ 50% AN goats. There was no (p > 0.05) significant difference in growth rates between 75% TN $\times$ 25% AN and 50% TN $\times$ 50 % AN goats. The growth rates of goats drenched every 3 weeks were significantly (p < 0.01) higher than those undrenched or drenched at 9 weeks. The results of this study also indicate that drenching alone did not result in increased weight gain except when the nutritional status was also improved. Parasitic infection affected some blood constituents, such as pack cell volume, haemoglobin, total protein and albumin. This resulted in lower growth rates for control groups and goats drenched at 9 weeks compared to those of goats drenched every 3 weeks.

Correlation Between Pulmonary Contusion and Myocardial Contusion in Patients with Multiple Injuries (다발성 외상환자에서 폐좌상과 심근좌상의 상관관계)

  • Ryu, Ji-Ho;Yeom, Seok-Ran;Jeong, Jin-Woo;Min, Mun-Ki;Park, Maeng-Real;Kim, Yong-In;Han, Sang-Kyun;Park, Sung-Wook
    • Journal of Trauma and Injury
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    • v.24 no.1
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    • pp.31-36
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    • 2011
  • Purpose: This study was conducted to evaluate the correlations among pulmonary contusion severity, trauma score and cardiac troponin I (cTnI) level. Methods: We prospectively evaluated patients with multiple injuries who had been admitted to the emergency department (ED) from July 2007 to July 2008. We first measured the total creatinine kinase (CK), the MB fraction of CK (CK-MB), TnI, and myoglobin within 2 hours after the injury. We then checked the electrocardiogram, x-ray, and computed tomography (CT) results. Finally, we assessed the injuries as variables and then compared the results for patients with elevated TnI levels (group A) and patients whose TnI levels fell within the normal range (group B). Results: Eighty-six of the 92 patients admitted to the ED were enrolled. The pulmonary contusion score (PCS) was well correlated with $PaO_2/FiO_2$. TnI levels were correlated with PCS. When TnI levels were above 0.86 ng/ml, the mortality was estimated with 100% sensitivity and 86.1% specificity. Conclusion: Pulmonary contusion severity is correlated with TnI level. When the PCS is high and the cTnI level is elevated in multiple-injury patients, we recommend continuous cardiac monitoring and further evaluation

Characteristics of Nutrient Concentrations in Groundwater under Paddy and Upland Fields (논과 밭 지하수의 영양물질 농도 특성)

  • Jang, Hoon;Kim, Jin-Soo;Kim, Young-Hyeon;Song, Chul-Min
    • Journal of The Korean Society of Agricultural Engineers
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    • v.53 no.6
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    • pp.67-74
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    • 2011
  • The objective of this study was to compare concentrations of nutrients such as total nitrogen (TN), nitrate nitrogen ($NO_3$-N) total phosphorous (TP), and phosphate phosphorous ($PO_4$-P) in groundwater under paddy and upland fields, and surface water recharging from a rural mixed land-use watershed. Chinese cabbage and hot pepper were cultivated on the upland field plot. The TN concentrations in upland groundwater showed double peaks (4.7, 4.3 mg/L, respectively) in April 2009 shortly after fertilizer application, indicating that TN concentrations are greatly influenced by fertilization. However, the TN concentrations in paddy groundwater were always lower than 2.0 mg/L irrespective of fertilization. Whereas the mean concentrations of TN and $NO_3$-N in upland groundwater significantly (p<0.05) higher than those in surface water, the mean concentrations of TP and $PO_4$-P in upland groundwater were significantly lower than those in surface water. On the other hand, the mean concentrations of TN, $NO_3$-N, TP and $PO_4$-P in paddy groudwater were significantly (p<0.05) lower than those in surface water. The TN concentrations in upland groundwater were generally higher than those in surface water during early April to early December due to the effect of fertilization, but vice versa in the other periods. In contrast, the TP concentrations in upland groundwater were always lower than those in surface water due to the sorption of inorganic phosphorous by soil. Moreover, the TN and TP concentrations in paddy groundwater were always lower than those in surface water, and therefore paddy groundwater may dilute nutrient concentrations in surface water when paddy groundwater and surface water mix.