• The Korean Journal of Physiology
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• 제1권1호
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• pp.77-82
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• 1967

The normal lung volumes were determined and subdivided under sitting position in 48 middle school girls, 49 high school girls and 44 house wives. All subjects were free of pulmonary and vascular diseases. The vital capacity was measured by Mckessons spirometer and the residual volume was determined by Rahn's three breathing method. 1. The lung volumes (BTPS) of middle school girls determined were: $RV\;0.59{\pm}0.11l\;FRC\;1.45{\pm}2.22l\;VC\;2.68{\pm}0.29l$ 2. The lung volumes (BTPS) of high school girls determined were: $RV\;0.83{\pm}0.19l\;FRC\;1.9{\pm}0.25l\;VC\;3.15{\pm}0.24l$ 3. The lung volumes (BTPS) of house wives determind were: $RV\;0.95{\pm}0.61{\ell}\;FRC\;2.1{\pm}0.25{\ell}\;VC\;3.06{\pm}0.29l$ 4. The calculated residual ratio $(RV/TLC{\times}100)$ were: $17.7{\pm}2.57%$ in middle school girls and $20.6{\pm}3.65%$ in high school girls and $24.0{\pm}2.31%$ in house wives 5. The functional residual ratio $(FRC/TLC{\times}100)$ were: $43.7{\pm}5.98%$ in middle school girls and $48.8{\pm}4.41%4 in high school girls and $52.6{\pm}5.38%$ in house wives. 6. The correlation coefficients between vital capacity and total lung capacity were r=0.96 in middle school girls and r=0.986 in high school girls and r=0.856 in house wives. 7. The regression equations were obtained follows: $TLC(l) =1.105{\times}VC+0.304$ (in middle school girls) $TLC(l) =1.551{\times}VC-0.902$ (in high school girls) $TLC(l) =0.999{\times}VC+0.954$ (in house wives)

• 한국항행학회논문지
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• 제18권2호
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• pp.128-133
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• 2014

본 논문은 TLC(telephone line controller)를 기반으로 한 BNWAS(bridge navigational watch alarm system) 연구이다. BNWAS의 기능과 동작은 국제 표준을 통해 제안되었으나, 현재 배에 탑재되어 있는 BNWAS는 모니터링하는 것에 많은 어려움을 가지고 있다. NMEA(national marine electronic association)-0183, NMEA-2000과 같은 BNWAS내의 장비에서 생성되는 여러 데이터가 있다. 비록 이러한 데이터가 배의 항해에 주로 사용되어지나, 센서를 통한 BNWAS 장비를 제어한다면 그 유용성은 더욱 증가할 것이다. 본 시스템의 취지는 선교의 항해 중 항해 당직자의 졸음 등으로 인한 해양사고를 예방하기 위한 것이다. 야간 항해 시 항해 당직자는 선교 내의 여러 장비를 통해 인근 선교의 여러 항행정보를 수집하고 지속적인 감시를 통하여 선박의 안전항해 여부를 결정하게 된다.

• Applied Biological Chemistry
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• 제30권1호
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• pp.88-94
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• 1987

인삼 성분중 생리적 활성을 가지고 있는 oligopeptide를 검출하고 분리하기 위하여 물 추출액을 ultra-filtration 한 후 gel filtration, ion-exchange chromatography 및 thin layer chromatography 등을 행하였다. 인삼 물 추출액으로부터 고분자 물질을 제거한 ultra-filtrate는 epinephrine에 의해 유도된 fat cell의 lipolysis를 저해하는 antilipolytic activity를 보유하고 있었으며 Sephadex G-25 gel filtration에 의해 3개 fraction으로 나뉘어졌다. 이중 첫번째 fraction(S-FI)만이 peptide 성분을 함유하고 있었으며 saponin 및 당도 검출되었다. S-FI fraction은 $Dowex\;50{\times}2\;ion-exchange\;chromatography$에 의하여 6개의 $fraction(P-F1{\sim}P-F6)$으로 분리되었고 TLC검정 결과 P-F2 fraction이 peptide fraction으로 antilipolytic를 보유하고 있었으며 TLC로 분리한 6개 spot는 각각 가수 분해한 전후의 TLC pattern을 비교해 본 결과 모두 oligopeptide임이 밝혀졌다. S-FI fraction에 존재한 saponine과 당은 P-F1 fraction 에서 모두 용출되었다.

• 분석과학
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• 제30권2호
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• pp.102-111
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• 2017

Here we reported a methodology for identification of triacylglycerols (TAGs) and diacylglycerols (DAGs) in coix seed by preparative thin layer chromatography (prep-TLC) and non-aqueous reversed-phase liquid chromatography (NARP LC)-atmospheric pressure chemical ionization (APCI) tandem mass spectrometry (MS/MS). Lipid components were extracted from coix seed by reflux extraction using n-hexane for 3 hr. TAGs and DAGs in coix seed extract were effectively purified and isolated from matrix interferences by prep-TLC and then analyzed by LC-APCI-MS and MS/MS for identification. TAGs were effectively identified taking into consideration of their LC retention behavior, APCI-MS spectra patterns, and MS/MS spectra of $[DAG]^+$ ions. In MS/MS spectra of TAGs, diacylglycerol-like fragment $[DAG]^+$ ions were useful to identify TAGs with isobaric fragment ions. Based on an established method, 27 TAGs and 8 DAGs were identified in coix seed extract. Among them, 15 TAGs and 8 DAGs were for the first time observed in coix seed. Interestingly, some of TAGs isolated by prep-TLC were partly converted into DAGs through probably photolysis process during storing in room temperature. Thus, degradation phenomenon of TAGs should be considered in the quality evaluation and nutritional property of coix seed. LC-APCI-MS/MS combined with prep-TLC will be practical method for precise TAG and DAG analysis of other herbal plants.

• 대한기계학회논문집B
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• 제20권10호
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• pp.3334-3343
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• 1996

A quantitative flow visualization technique was developed to measure velocity and temperature fields simultaneously in a two-dimensional cross section of thermo-fluid flows. Thermochromic liquid crystal(TLC) particles are used as temperature sensor and velocity tracers. Illuminating a thermo-fluid flow with a thin sheet of white light, the reflected colors from the TLC particles in the flow were captured simultaneously by two CCD cameras; a 3-chip CCD color camera for temperature field measurement and a black and white CCD camera for velocity field measurement. Variations of temperature field were measured by using a HSI true color image processing system and TLC solution. The relationship between the hue values of TLC color image and real temperature was obtained and this calibration curve was used to measure the true temperature under the same camera and illumination condition. The velocity field was obtained by using a 2-frame PTV technique using the concept of match-probability to track true velocity vectors from two consecutive image frames. These two techniques were applied at the same time to the unsteady thermal-fluid flow in a Hele-Shaw cell to measure the temperature and velocity field simultaneously and some results are discussed.

• 한국해양바이오학회지
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• 제1권4호
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• pp.298-304
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• 2006

In this study, we establish a thin-layer chromatography (TLC) immunostaining method for detecting starfish gangliosides. A new monoclonal antibody (MAb) against AG-2, the major gangliosides molecular species of Acanthaster planci, was produced by fusing hybridoma with splenocytes immunized to liposomal AG-2. BALB/c male mice were injected with liposomal AG-2 antigen, and immunized. Their splenocytos were isolated and fused with hypoxanthine-aminopterine-thimidine (HAT)-sensitive mouse myeloma cells. Hybridomas producing MAb reactive to AG-2 were cloned using the limited dilution method. Established hybridomas were cultured in eRDF medium. Crude MAb produced from clone 8D4 was purified with a magnesium pyrophosphate column. Enzyme immunoassay and TLC immunostaining of AG-2 were performed using the purified MAb. Structurally related gangliosides did not cross-react with anti-AG-2 antibodies. The detection limit of TLC immunostaining was 50 ng of AG-2. The newly established immunostaining method was further developed for detecting AG-2 distribution and qualitative analysis in tissues and/or organs. Our results show that the majority of AG-2 is present in the stomach of male A. planci, while AG-2 is distributed not only in the stomach but also in the the pyloric caeca of female A. planci.

• 한국동물위생학회지
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• 제20권3호
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• pp.313-321
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• 1997

The antibiotic residues of the urine, the liver, the lung, the kidney and the spleen in slaughtered pigs at Kyongbuk province were detected by TLC(505 kit) and EEC-4 plate method. 1. The positive rate of residual sulfamethazine which was detected by 505 kit in the urine (n=200) was 0.0%. 2. The positive rate of residual sulfamethazine which was detected by EEC-4 plate in the urine (n=126), the liver(n=98), the kidney(n=72), the spleen (n=68) and the lung(n=48) were 63%, 49%, 36%, 34% and 24%, respectively. 3. By EEC-4 plate method, the positive detection rates of the urine were 53.0% in BS(pH 6.0), 29.0% in BS(pH 7.2), 11.5% in BS(pH 8.0) and 13.0% in ML(pH 8.0) medium, that of the liver 41.5% in BS(pH 6.0), 22.0% in BS(pH 7.2), 6.5% in BS(pH 8.0) and 5.0%, in ML (pH 8.0) medium, that of the lung 21.0% in BS(pH 6.0), 9.5%, in BS(pH 7.2) and 8.5% in BS(pH 8.0) medium, and that of the kidney 31.5% in BS(pH 6.0), 14.5% in BS(pH 7.2), 20.0% in BS(pH 8.0) and 3.0% in ML(pH 8.0) medium. In the spleen, only in BS(pH 6.0) medium the positive rate was detected as 33.5 %. 4. The positive rates of samples which shown TLC-positive detected by EEC-4 plate method were 53.9% in no band, 77.8% in one band, 80.9% in two bands, 66.7% in three bands, respectively. In conclusion, the EEC-4 plate method could be applied for the detection of residual antibiotics in samples which shown as out of standard Rf values by TLC-method (SOS kit).

• 디지털콘텐츠학회 논문지
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• 제10권2호
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• pp.233-238
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• 2009

디지털 통신 네트워크 기술의 발전에서 인터넷 기술과 스마트 홈 가전기기의 확산은 고속/고품질 홈 네트워크에 대한 필요성을 증가시키고 있다. 홈 네트워크에서 가전기기와 컴퓨팅 기기가 증가함에 따라 홈 네트워크의 데이터 트래픽은 증가할 것이 명백하다. 다양한 홈 네트워크 기기는 멀티미디어 콘텐츠를 얻기 위하여 인터넷 서버에 접속하길 원한다. 따라서 본 논문에서는 가정내에서 이더넷이나 유무선 기술을 사용하기 위해 네트워크에 연결된 디지털 가전기기를 위한 TLC(Telephone Line Carrier) 시스템을 소개한다. 미래의 홈 네트워크 환경에서 TLC 기반의 스마트 홈 네트워크의 주된 목적은 저가격, 쉬운 설치, 고성능, 넓은 영역을 만드는 것이다. 본 논문에서는 스마트 홈 네트워크를 위한 전화선로 통신시스템의 채널용량을 송신전력, OFDM 반송파의 수, 채널손실, 잡음손실에 따라서 정량적으로 비교, 분석하였다.

• Journal of Ginseng Research
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• 제14권3호
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• pp.442-446
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• 1990

As a part of studies on the quality control of crude drug-drink preparations, ginger components were identified by TLC and 6-gingerol was determined quantitatively by HPLC. Ginger components were identified by TLC with benzene/acetone (4:1, v/v, on silica gel plate by spraying a vanillinsulfuric acid reagent. 5-Gingerol contents were determined at 280 nm by HPLC on Lichro CART RP-18 column with acetonitrile/wate(38:62, v/v). Its transfer rate in the 3 types of crude drug extract drinks was 65.4-85.1% compared to the content in the ginger extract.

• 한국환경보건학회지
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• 제27권4호
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• pp.35-40
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• 2001

In order to evaluate the safety of agricultural products in Korea, we carried out work by screening of Fusarium species. which can produce deoxinivalenol(DON) from agricultural products in Western Gyeongnam, Korea. From 215 samples of soil agricultural products, 129 strains of Fusarium species were obtained. The production of DON was verified by thin layer chromatography(TLC) As the results of TLC, 25 strains were identified as DON producing strain. But only 10 strains were identified as DON producing strains by enzyme-linked immunosorbent assay (ELISA). The maximum DON producing strain No.41 was isolated from corn. In conclusion. the above results indicate that DON producing fungi contaminated agricultural products in Korea. Therefore further studies are required to accumulate more detailed data about the contamination of DON in various agricultural products.