• Journal of Applied Biological Chemistry
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• 제60권4호
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• pp.351-355
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• 2017

톨라신은 Pseudomonas tolaasii에 의해 생성되는 펩티드 독소이며, 인공재배 버섯에 갈반병을 일으킨다. 톨라신 펩티드는 막에 pore를 형성하고, 세포막 구조를 파괴한다. 톨라신의 분자 작용은 톨라신 분자들의 응집, 세포막 결합, 세포막에서의 pore 형성으로 이루어져 있다. 따라서, 이 작용의 억제는 갈반병을 억제할 수 있다. 식품첨가물로부터 몇몇의 톨라신 저해제(tolaasin inhibitory factors, TIF)를 얻었다. TIF는 버섯에 감염된 병원균에 의한 갈반 형성을 억제할 수 있었다. 본 연구에서는 TIF를 다양한 조건에서 보관하였으며, 톨라신에 의한 세포 독성의 저해에 대해 이들의 활성을 조사하였다. TIF는 불포화탄소 화합물이기 때문에 대기 노출과 빛 조사에 민감하다. 혐기적 조건에서 TIF는 3개월동안 안정하였고, 효과는 10% 정도 감소하였다. 그러나 빛과 공기와 접촉한 조건에서는 2주동안 90% 가까이 TIF 활성이 억제되었다. 5, 25, $45^{\circ}C$의 저장온도에서는 어떠한 차이도 보이지 않았기 때문에 온도는 TIF의 안정성에 유의적인 영향을 끼치지 않았다. 따라서, TIF 화합물의 안정적인 저장을 위해서는 용기는 밀폐되고, 빛은 차단되어야 한다.

• 토지주택연구
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• 제6권4호
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• pp.185-193
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• 2015

수도권 신도시가 도입된 이래 신도시 분양가는 지속적으로 상승하였는데, 그 주된 원인 중의 하나는 광역교통시설 설치비용의 과도한 부담이다. 광역교통시설은 신도시 외에도 그 인근 지역이 사용하는 시설임에도 불구하고 지자체의 부담 없이 사업자에게 전가되고 있는 실정이다. 이에 본 연구에서는 동탄신도시를 사례로 광역교통시설의 통행수요 분석을 통해 지자체와 사업자간 합리적인 비용분담구조를 도출하고 그 재원을 조달하기 위한 방안으로서 조세담보금융(TIF)의 적용방안을 제시하였다. 통행수요 분석결과, 신도시 기점 가중통행량 비율은 35.1%로 나타났으며, 이에 따라 지방정부가 부담해야할 사업비는 약 8000억원으로 도출되었다. 이와 같은 규모의 재원을 재산세만을 활용해 지자체가 조달하기 위한 TIF 운영은 적어도 20년의 조달기간과 부동산가치 증가율 2%가 필요한 것으로 분석되었다.

• 대한기관식도과학회지
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• 제18권2호
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• pp.56-59
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• 2012

Tracheo-innominate artery fistula (TIF) is a rare but catastrophic and almost always fatal complication of tracheostomy. TIF can occur anytime but is commonly present 3 to 24 days after tracheostomy. It can first manifest as massive bleeding around and through the tracheostomy tube, but it can also manifest as a small amount of blood with temporary spontaneous resolution. If TIF is suspicious, airway management and prompt surgical intervention are needed. In an 83-year-old man with CVA history 20 years earlier and who had recurrent aspiration pneumonia, tracheostomy was performed for respiratory management and ventilator support. On day 7 post-tracheostomy, the patient had bleeding from the tracheostoma. Immediate surgical exploration was performed to control the bleeding. A defect was seen at the post wall of the innominate artery. The erosive portion of the artery was sutured, but the patient died three weeks after the surgery due to rebleeding and respiratory failure. We present a patient who developed TIF after tracheostomy, with literature review.

• International Journal of Safety
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• 제9권2호
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• pp.16-21
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• 2010

In this study, the variations of transformed impact factors and load carrying capacity of highway bridges measured from the state of expansion joint are evaluated. the field loading tests were performed on the highway bridge with damaged expansion joint to investigate the variation of the load carrying capacity. From the field loading tests in case that damaged expansion joint exist or do not exist, the static displacements and dynamic displacements were measured, and TIF were calculated, respectively. dynamic test is performed in order to estimate dynamic displacement and TIF according to the level of damage of expansion joint. From the results of TIF, the load carrying capacity of highway bridges with damaged expansion joint were estimated.

• Biomolecules & Therapeutics
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• 제29권2호
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• pp.234-247
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• 2021

We used a heterozygous gene deletion library of fission yeasts comprising all essential and non-essential genes for a microarray screening of target genes of the antifungal terbinafine, which inhibits ergosterol synthesis via the Erg1 enzyme. We identified 14 heterozygous strains corresponding to 10 non-essential [7 ribosomal-protein (RP) coding genes, spt7, spt20, and elp2] and 4 essential genes (tif302, rpl2501, rpl31, and erg1). Expectedly, their erg1 mRNA and protein levels had decreased compared to the control strain SP286. When we studied the action mechanism of the non-essential target genes using cognate haploid deletion strains, knockout of SAGA-subunit genes caused a down-regulation in erg1 transcription compared to the control strain ED668. However, knockout of RP genes conferred no susceptibility to ergosterol-targeting antifungals. Surprisingly, the RP genes participated in the erg1 transcription as components of repressor complexes as observed in a comparison analysis of the experimental ratio of erg1 mRNA. To understand the action mechanism of the interaction between the drug and the novel essential target genes, we performed isobologram assays with terbinafine and econazole (or cycloheximide). Terbinafine susceptibility of the tif302 heterozygous strain was attributed to both decreased erg1 mRNA levels and inhibition of translation. Moreover, Tif302 was required for efficacy of both terbinafine and cycloheximide. Based on a molecular modeling analysis, terbinafine could directly bind to Tif302 in yeasts, suggesting Tif302 as a potential off-target of terbinafine. In conclusion, this genome-wide screening system can be harnessed for the identification and characterization of target genes under any condition of interest.

• 만화애니메이션 연구
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• 통권14호
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• pp.37-51
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• 2008

애니메이션 작업에 있어 컴퓨터는 꼭 필요한 도구가 되었다. 그러나 컴퓨터와 그에 따른 소프트웨어들의 특성을 잘 파악하지 못할 경우 노력에 상응하는 결과물을 만들지 못할 수도 있다. 이미지 포맷의 부정확한 이해에서 이러한 경우가 발생하기도 한다. 대다수 작업자들은 제작공정 속에서 이미지 포맷을 습관적으로 선택하는 경우가 많으나, 이미지 포맷은 분명한 기능적 차이를 가지고 있으며 그 효율적 사용법도 다르다. 그러므로 보다 효율적인 작업을 위해 애니메이션 작업에 주로 사용되는 여러 종류의 이미지 포맷들을 특성을 파악하는 것이 필요하다. 먼저 오늘날 통신, 데이터저장 등 컴퓨터 전반에서 널리 쓰이는 데이터 압축의 두 종류인 손실 압축과 비손실 압축의 원리와 이미지표현의 서로 다른 구조인 비트맵 방식과 벡터 방식의 차이점, 24비트 트루 컬러와 8피트 알파채빌 등을 살펴보았다. 이러한 특성들을 토대로 2D, 3D, 합성, 편집 등 여러 형태의 애니메이션 작업 간에 사용되는 이미지 포맷들의 기능적 차이점과 그에 따른 장단점을 분석하였으며 실험을 통하여 JPG 파일이 컴퓨터 작업 중에 적은 용량을 차지할 것이라는 생각이 잘못된 것임을 증명하였다. 결론적으로 용량, 기능, 화질을 고려할 때, 편집, 합성, 3D, 2D를 막론하고 가장 효율적인 포맷으로는 TIF 포맷을 제안하며 학교 교육에서는 어도비사의 교육용 프로그램을 많이 사용하므로 호환성과 기능이 우수한 PSD 포맷을 제안하였다. 본 논문이 학교 교육에서나 실무 작업에서 이미지 포맷을 선택함에 있어 기준이 되기를 기대한다.

• Journal of Oral Medicine and Pain
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• 제21권1호
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• pp.7-14
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• 1996

This study was performed to compare the anticarious effects of different fluoride agents(NaF, $NH_4F, TIF_4,$ APF gel, Elmex gel ) on artificial carious lesion. Enamel samples treated with 5 kinds of fluoride agents by pH cycling method were evaluated for fluorine uptake, enamel solubility, and microhardness. The results were as follows : 1. Greater fluoride uptakes were obtained in carious lesion treated with APF gel and $TiF_4$ solution than in that treated with other fluoride solutions. 2. TiF4 group was more resistant to acid than otter groups, but it was not significantly different with APF gel group. 3. Surface of enamel specimen was harkened by fluoride application and pH cycling. APF gel was more effective on enhancing surface hardness than control group. 4. APF gel, $NH_4F, TIF_4,$ solution were effective on fluoride uptake, enamel solubility, and microhardness.

• 한국환경성돌연변이발암원학회지
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• 제22권3호
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• pp.175-182
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• 2002

Endocrine disruptors (EDs) are the chemicals that affect endocrine systems through activation or inhibition of steroid hormone response. It is necessary to have a good system to evaluate rapidly and accurately endocrine-disrupting activities of suspected chemicals and their degradation products. The key targets of EDs are nuclear hormone receptors, which bind to steroid hormones and regulate their gene transcription. We constructed a co-expression system of Gal4p DNA binding domain (DBD)- ligand binding domain of human estrogen receptor $\alpha$ or $\beta$, and Gal4p transactivation domain (TAD)-co-activator AIB-1, SRC-1 or TIF-2 in Saccharomyces cerevisiae with a chromosome-integrated lacZ reporter gene under the control of CYC1 promoter and Gal4p binding site (GAL4 upstream activating sequence, GAL4$_{UAS}$). Expression of this reporter gene was dependent on the presence of estrogen or EDs in the culture medium. We found that the two-hybrid system with combination of the hER$\beta$ LBD and co-activator SRC-1 was most effective in the xenoestrogen-dependent induction of reporter activity. The extent of transcriptional activation by those chemicals correlated with their estrogenic activities measured by other assay systems, indicating that this assay system is efficient and reliable for measuring estrogenic activity. The data in this research demonstrated that the yeast detection system using steroid hormone receptor and co-activator is a useful tool for identifying chemicals that interact with steroid receptors.s.

• Journal of Chest Surgery
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• 제20권1호
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• pp.218-222
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• 1987

Unroofed coronary sinus syndrome is an uncommon anomaly, Unroofed coronary sinus syndrome is caused by incomplete formation of the left atriovenous fold, and it usually is associated with Left SVC. If it is not diagnosed, a residual reversed or bidirectional shunt will result, and its complications will reduce life expectancy. We experienced 3 cases of unroofed coronary sinus syndrome which combines TOF with PLSVC, partial ECD, primum type ASD. In case of Unroofed coronary sinus syndrome which combines TIF with PLSVC, preoperative diagnosis was not made. In corrective operation for TOF of this case, pump weaning was failed due to hypoxia and cardiac arrest, and he expired at operation room. At autopsy of this case, complete unroofed coronary sinus was found. In the other 2 cases, partial unroofed coronary sinus syndrome was found in operation field and corrective operation was performed successfully. We report these 3 experiences with its review. ^u ++ Noninvasive Assessment of Pressure Gradients across Prosthetic Heart Valve by Doppler Ultrasound - A comparative study of the Duromedics Bileaflet Valves in mitral position and Normal Mitral Valves -with its review.

• 대한수의학회지
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• 제52권2호
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• pp.99-104
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• 2012

The present study was performed to evaluate the relationship between the neurotoxicity of acrylamide and the differential gene expression pattern in mice. Both locomotor test and rota-rod test showed that the group treated with higher than 30 mg/kg/day of acrylamide caused impaired motor activity in mice. Based on cDNA microarray analysis of mouse brain, myelin basic protein gene, kinesin family member 5B gene, and fibroblast growth factor (FGF) 1 and its receptor genes were down-regulated by acrylamide. The genes are known to be essential for neurofilament synthesis, axonal transport, and neuroprotection, respectively. Interestingly, both FGF 1 and its receptor genes were down-regulated. Genes involved in nucleic acid binding such as AU RNA binding protein/enoyl-coA hydratase, translation initiation factor (TIF) 2 alpha kinase 4, activating transcription factor 2, and U2AF 1 related sequence 1 genes were down-regulated. More interesting finding was that genes of both catalytic and regulatory subunit of protein phosphatases which are important for signal transduction pathways were down-regulated. Here, we propose that acrylamide induces neurotoxicity by regulation of genes associated with neurofilament synthesis, axonal transport, neuro-protection, and signal transduction pathways.